Dr Anita Horvath - Researcher Profile

Anita Horvath

Address

Australian Centre for Blood Diseases
6th Floor, Burnet Tower, The Alfred Hospital

Biography

Anita works in the Department of Clinical Haematology and Australian Centre for Blood Diseases within the Faculty of Medicine, Nursing & Health Sciences at Monash University as a Research Fellow.

 Dr Anita Horvath is a postdoctoral research fellow at the Australian Centre for Blood Diseases and is investigating the structural and functional interactions of proteases and their inhibitors, serpins. Her work is focused on examining how the key fibrinolytic protease, plasmin interacts with its natural inhibitor antiplasmin. She is applying her extensive knowledge in fibrinolysis regulation to develop new therapeutics to dissolve pathological blood clots. Dr Horvath is also involved in collaborations with structural biologists at Department of Biochemistry and Molecular Biology, Monash University. Her work has contributed to the elucidation of the X‑ray crystal structures of antichymotrypsin, anitplasmin, plasminogen and has been published in top ranking journals including Journal of Experimental Medicine, Cell Reports, Blood and the Journal of Biological Chemistry. Dr Horvath is also actively involved in the undergraduate teaching of BSc and BMS as unit co-ordinator of the Human Pathology units and has supervised and mentored Honours and PhD students.  

Qualifications

MEDICINE
Institution: Monash University
Year awarded: 2005

Publications

Book Chapters

Horvath, A.J., Lu, B.G., Pike, R.N., Bottomley, S.P., 2011, Methods to measure the kinetics of protease inhibition by serpins, in Methods in Enzymology, Volume 501: Serpin Structure and Evolution, eds James C Whisstock and Phillip I Bird, Academic Press, USA, pp. 223-235.

Journal Articles

Law, R.H., Caradoc-Davies, T.T., Cowieson, N.P., Horvath, A.J., Quek, A.J., Amarante Da Encarnacao, J., Steer, D.L., Cowan, A.D., Zhang, Q., Lu, B.G., Pike, R.N., Smith, A.I., Coughlin, P.B., Whisstock, J.C., 2012, The x-ray crystal structure of full-length human plasminogen, Cell Reports [E], vol 1, issue 3, Cell Press, USA, pp. 185-190.

Young, L., Birch, N., Browett, P.J., Coughlin, P.B., Horvath, A.J., van de Water, N.S., Ockelford, P.A., Harper, P.L., 2012, Two missense mutations identified in venous thrombosis patients impair the inhibitory function of the protein Z dependent protease inhibitor, Thrombosis and Haemostasis [P], vol 107, issue 5, Schattauer GmbH, Germany, pp. 854-863.

Lu, B.G.C., Sofian, T., Law, R.H., Coughlin, P.B., Horvath, A.J., 2011, Contribution of conserved lysine residues in the alpha{2}-antiplasmin C terminus to plasmin binding and inhibition, Journal Of Biological Chemistry [P], vol 286, issue 28, American Society for Biochemistry & Molecular Biology, Inc., USA, pp. 24544-24552.

Niego, B., Horvath, A.J., Coughlin, P.B., Pugsley, M.K., Medcalf, R.L., 2008, Desmoteplase-mediated plasminogen activation and clot lysis are inhibited by the lysine analogue tranexamic acid, Blood Coagulation and Fibrinolysis, vol 19, issue 4, Lippincott Williams & Wilkins, United States, pp. 322-324.

Law, R.H.P., Sofian, T., Kan, W., Horvath, A.J., Hitchen, C.R., Langendorf, C., Buckle, A.M., Whisstock, J., Coughlin, P.B., 2008, X-ray crystal structure of the fibrinolysis inhibitor alpha2-antiplasmin, Blood, vol 111, American Society of Hematology, Washington, DC, USA, pp. 2049-2052.

Paterson, M.A., Horvath, A.J., Pike, R.N., Coughlin, P.B., 2007, Molecular characterization of centerin, a germinal centre cell serpin, Biochemical Journal, vol 405, issue 3, Portland Press Ltd, UK, pp. 489-494.

Winkler, I.G., Hendy, J.A., Coughlin, P.B., Horvath, A.J., Levesque, J., 2005, Serine protease inhibitors serpina1 and serpina3 are down-regulated in bone marrow during hematopoietic progenitor mobilization, The Journal of Experimental Medicine, vol 201, issue 7, The Rockefeller University Press, USA, pp. 1077-1088.

Horvath, A.J., Irving, J.A., Rossjohn, J., Law, R.H.P., Bottomley, S.P., Quinsey, N.S., Pike, R.N., Coughlin, P.B., Whisstock, J., 2005, The murine orthologue of human antichymotrypsin: a structural paradigm for CLADE A3 serpins, Journal of Biological Chemistry, vol 280, issue 52, American Society for Biochemistry and Molecular Biology, Inc., Bethesda USA, pp. 43168-43178.

Horvath, A.J., Forsyth, S.L., Coughlin, P.B., 2004, Expression Patterns of Murine Antichymotrypsin-like Genes Reflect Evolutionary Divergence at the Serpina3 Locus, Journal of Molecular Evolution, vol 59, issue 4, Springer-Verlag, USA, pp. 488-497.

Forsyth, S.L., Horvath, A.J., Coughlin, P.B., 2003, A review and comparison of the murine a1-antitrypsin and a1 -antichymotrypsin multigene clusters with the human clade A serpins, Genomics, vol 81, Academic Press Inc. Elsevier Science, San Diego USA, pp. 336-345.

Morris, E.C., Dafforn, T.R., Forsyth, S.L., Missen, M.A., Horvath, A.J., Hampson, L., Hampson, I.N., Currie, G., Carrell, R.W., Coughlin, P.B., 2003, Murine serpin 2A is a redox-sensitive intracellular protein, Biochemical Journal, vol 371, issue 1, Portland Press, London UK, pp. 165-173.

Other

Sofian, T., Horvath, A.J., Law, R.H.P., Whisstock, J., 2007, Monash Researchers Fight Deep Vein Thrombosis, Media Release 13 December 2007, Monash University, Australia.

Teaching Commitment

Unit Co-ordinator for HUP3011 and HUP3022.

Grants

Title:
Development of a new therapeutic agent to dissolve pathological blood clots.
Investigators:
Horvath, A
Funding:
(2013 - 2016). CASS Foundation Limited.

Postgraduate Research Supervisions

Current Supervision

Program of Study:
(DOCTORATE BY RESEARCH).
Thesis Title:
Structural and functional analysis of the interaction of plasmin lysine binding sites with antiplasmin C-terminus.
Supervisors:
Coughlin, P (Main), Horvath, A (Associate).

Completed Supervision

Student:
Sofian, T.
Program of Study:
Structural and functional characterisation of the serpin; alpha2- antiplasmin. (PHD) 2009.
Supervisors:
Coughlin, P (Main), Horvath, A (Associate), Medcalf, R (Associate).