Prof Suzanne Crowe - Researcher Profile

Biography

Other Appointments (current)

1989 - now    Head, Pathogenesis and Clinical Research, Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne
1989 - now    Head, Flow Cytometry Laboratory, Macfarlane Burnet Institute for Medical Research and Public Health
1992 - now    Head, Clinical Research Laboratory, Macfarlane Burnet Institute for Medical Research and Public Health
1994 - now    Consultant Physician in Infectious Diseases and General Medicine, Alfred Hospital, Melbourne
1999 - now    Professor of Medicine and Infectious Diseases (Personal Chair), Monash University, Melbourne              
2002 - now    NHMRC Principal Research Fellow

 

Academic Background

1968-73    Medical Laboratory Scientist (Microbiology), Repatriation General Hospital, Heidelberg.
1980-83    Intern, Medical Resident, Medical Registrar, Senior Medical Registrar, Monash University Department of Medicine, Alfred Hospital, Melbourne
1984-85   Medical Registrar in Infectious Diseases, Fairfield Hospital, Melbourne
1986-88   Research Fellow in Infectious Diseases, University of California, San Francisco
1998        Sabbatical, Gladstone Institute, University of California, San Francisco

 

Previous Positions

1989-1995    Senior Lecturer, Monash University Department of Medicine
1989-1996    Sessional Senior Infectious Diseases Physician, Fairfield Hospital
1992-2003    Principal Investigator, National Centre in HIV Virology Research
1995-1999    Associate Professor, Monash University, Department of Medicine
1997-2003    Consultant Physician, Professorial General Medical Unit,Alfred Hospital

 

Patents and Commercial Development

Australian Provisional Patent Application No. 2006905393 “A method of diagnosis and kit therefor” filed 28 September 2006 in the name of the Burnet Institute. Inventors:  Anderson D, Crowe S, Landay, A and Lloyd, R.

 

Professional Membership

Fellow, Royal Australasian College of Physicians
Fellow, Infectious Diseases Society of America
Member, Australasian Society for Infectious Diseases
Member, Australasian Society for HIV Medicine
Member, American Association for the Advancement of Science
Member, American Society of Microbiology
Member, Forum for Collaborative HIV Research

 

Awards

1977 - Jean C Tolhurst Memorial Prize for Microbiology
1979 - Mead-Johnson Prize for Paediatrics
1986 - Merck, Sharp and Dohme Travelling Scholarship
1986-88 Harkness Foundation Fellowship
1987 - International Award for AIDS Research (World Federation of Contraception/Health)
1988 - Beecham Research Award (Royal Australasian College of Physicians
1988 - Fogarty Travelling Fellowship (to attend IV Int Conf on AIDS), Stockholm
1989-93  Wellcome Trust Senior Research Fellowship
1991 - Glaxo Award for Advanced Research in Infectious Diseases
1993 - Society for Leukocyte Biology Award for International Advancement of   the Science of AIDS
1994-96 Commonwealth AIDS Research Grants Career Award
1997 - Inaugural Award for Outstanding Past Pupil of Genazzano College
1997 - Royal Australian College of Physicians, The Macquarie Bank Travelling Fellowship.
2005 - Life time Achievement Award, 5th International Conference on AIDS, India
2006 - Inaugural Frank Fenner Award, Burnet Institute, Melbourne.

Research & Supervision Interests

Role of Macrophages in HIV Pathogenesis
We are interested in the role of the CD16+ monocyte subset in the development of viral reservoirs in HIV infected persons.  This subset is more susceptible to HIV infection than the majority of monocytes in peripheral blood.  We are examining the transendothelial migration of HIV infected and uninfected monocytes to understand the establishment of viral reservoirs.  We are also studying whether matrix metalloproteinase production is altered as a result of HIV infection and whether this contributes to altered permeability of tissue-endothelial barriers. Having demonstrated that macrophages are more susceptible to HIV infection than monocytes, we have been actively studying the effects of HIV on phagocytic capacity of macrophages, and mechanisms underlying the HIV-induced reduction in their function.

Low Cost Assays for Monitoring HIV Infection in Resource Constrained Settings
We are evaluating low cost CD4 (Dynal Dyna beads) and low cost viral load (Cavidi ExaVir) methods, as well as transferring these technologies into laboratories in resource constrained countries.  We are developing a low cost, rapid, semi-quantitative CD4 test to be used in remote settings without laboratory access, for estimation of CD4 counts.  We are studying dried blood spot and dried plasma spot assays for viral load and resistance measurement.

Qualifications

DOCTOR OF PHILOSOPHY

Institution: Monash University

Year awarded: 2005

Publications

Book Chapters

Ryan, C.E., Oelrichs, R., Crowe, S.M., 2009, Virological diagnostics, in HIV Management in Australasia: A Guide for Clinical Care, eds Jennifer Hoy, Sharon Lewin, Jeffrey J Post and Alan Street, Australian Society for HIV Management, Australia, pp. 49-57.

Jaworowski, A., Crowe, S.M., 2007, Mechanisms of HIV impairment of phagocytosis by monocytes and macrophages, in HIV and the Macrophage, eds Georges Herbein, Transworld Research Network, India, pp. 159-182.

Priya, R., Kumarasamy, N., Qadeer, I., Ganesh, A.K., Solomon, S.S., Crowe, S.M., 2006, India, in The HIV Pandemic - Local and Global Implications, eds Eduard J. Beck, Nicholas Mays, Alan W Whiteside and José M Zuniga, Oxford University Press, USA, pp. 300-316.

Gorry, P.R., Sonza, S., Kedzierska, K., Crowe, S.M., 2005, Isolation of human immunodeficiency virus Type 1 from peripheral blood monocytes, in Methods in Molecular Biology - Human Retrovirus Protocols: Virology and Molecular Biology, eds Tuofu Zhu, Humana Press Inc., USA, pp. 25-33.

Ellery, P., Crowe, S.M., 2005, Phenotypic characterization of blood monocytes from HIV-infected individuals, in Methods in Molecular Biology - Human Retrovirus Protocols: Virology and Molecular Biology, eds Tuofu Zhu, Humana Press Inc, USA, pp. 343-353.

Crowe, S.M., Dwyer, D.E., Cunningham, A.L., Mills, J.F., 2004, Antiviral agents, in Infectious Diseases: A Clinical Approach, eds Allen Yung, Malcolm McDonald, Denis Spelman, Alan Street, Paul Johnson, Tania Sorrell and Joe McCormack, IP Communications, Australia, pp. 45-65.

Crowe, S.M., Maslin, C.L., Ellery, P., Kedzierska, K., 2004, Culture of HIV in monocytes and macrophages, in Current Protocols in Immunology, eds John E. Coligan, Barbara Bierer, David H.Margulies, Ethan M. Shevach, Warren Strober and Richard Coico, John Wiley & Sons, Inc., USA, pp. 12.4.1-12.4.13.

Journal Articles

Lichtfuss, G., Hoy, J., Rajasuriar, R., Kramski, M., Crowe, S., Lewin, S., 2011, Biomarkers of immune dysfunction following combination antiretroviral therapy for HIV infection, Biomarkers In Medicine [P], vol 5, issue 2, Future Medicine Ltd., United Kingdom, pp. 171-186.

Tippett, E., Cheng, W., Westhorpe, C., Cameron, P., Brew, B., Lewin, S., Jaworowski, A., Crowe, S., 2011, Differential expression of CD163 on monocyte subsets in healthy and HIV-1 infected individuals, PLoS ONE [P], vol 6, issue 5 (Art. No: e19968), Public Library of Science, United States, pp. 1-11.

Hearps, A., Angelovich, T.A., Jaworowski, A., Mills, J., Landay, A.L., Crowe, S.M., 2011, HIV infection and aging of the innate immune system, Sexual Health [P], vol 8, issue 4, C S I R O Publishing, Australia, pp. 453-464.

Kramski, M., Gaeguta, A.J., Lichtfuss, G.F., Rajasuriar, R., Crowe, S., French, M.A., Lewin, S.R., Center, R.J., Purcell, D.F.J., 2011, Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation, Journal Of Clinical Microbiology [P], vol 49, issue 10, American Society for Microbiology, United States, pp. 3691-3693.

Chang, C., Leslie, D.E., Spelman, D.W., Chua, K., Fairley, C.K., Street, A., Crowe, S., Hoy, J.F., 2011, Symptomatic and asymptomatic early neurosyphilis in HIV-infected men who have sex with men: a retrospective case series from 2000 to 2007, Sexual Health [P], vol 8, issue 2, C S I R O Publishing, Australia, pp. 207-213.

Cornall, A., Sharma, L., Solomon, A., Gorry, P., Crowe, S., Cameron, P.U., Lewin, S.R., 2010, A novel, rapid method to detect infectious HIV-1 from plasma of persons infected with HIV-1, Journal of Virological Methods [P], vol 165, issue 1, Elsevier BV, The Netherlands, pp. 90-96.

Rajasuriar, R., Booth, D., Solomon, A., Chua, K., Spelman, T., Gouillou, M., Schlub, T., Davenport, M., Crowe, S., Elliott, J., Hoy, J., Fairley, C., Stewart, G., Cameron, P., Lewin, S., 2010, Biological determinants of immune reconstitution in HIV-infected patients receiving antiretroviral therapy: The role of interleukin 7 and interleukin 7 receptor alpha and microbial translocation, Journal Of Infectious Diseases [P], vol 202, issue 8, Oxford University Press, USA, pp. 1254-1264.

Wightman, F., Solomon, A., Khoury, G., Green, J., Gray, L., Gorry, P.R., Ho, Y., Saksena, N., Hoy, J.F., Crowe, S., Cameron, P., Lewin, S., 2010, Both CD31+and CD31- naive CD4+ T cells are persistent HIV type 1-infected reservoirs in individuals receiving antiretroviral therapy, Journal of Infectious Diseases [E], vol 202, issue 11, Oxford University Press, United States, pp. 1738-1748.

Leeansyah, E., Zhou, J., Paukovics, G., Lewin, S.R., Crowe, S.M., Jaworowski, A., 2010, Decreased NK cell FcRgamma in HIV-1 infected individuals receiving combination antiretroviral therapy: A cross sectional study, PLoS ONE [P], vol 5, issue 3 (Art. No: e9643), Public Library of Science, USA, pp. 1-8.

Lichtfuss, G., Meehan, A., Cheng, W., Cameron, P., Lewin, S., Crowe, S., Jaworowski, A., 2010, HIV inhibits early signal transduction events triggered by CD16 cross-linking on NK cells, which are important for antibody-dependent cellular cytotoxicity, Journal of Leukocyte Biology [P], vol 89, Federation of American Societies for Experimental Biology, USA, pp. 149-158.

Anderson, D.A., Crowe, S.M., Garcia, M., 2010, Point-of-Care Testing, Current HIV/AIDS Reports [E], vol 8, issue 1, Current Medicine Group LLC, USA, pp. 31-37.

Maisa, A., Westhorpe, C., Elliott, J., Jaworowski, A., Hearps, A., Dart, A., Hoy, J., Crowe, S., 2010, Premature onset of cardiovascular disease in HIV-infected individuals: the drugs and the virus, Future HIV Therapy [P], vol 4, issue 6, Future Medicine Ltd, United Kingdom2010, pp. 675-692.

Crowe, S., Westhorpe, C., Mukhamedova, N., Jaworowski, A., Sviridov, D., Bukrinsky, M., 2010, The macrophage: the intersection between HIV infection and atherosclerosis, Journal of Leukocyte Biology [P], vol 87, Federation of American Societies for Experimental Biology, United States, pp. 589-598.

Hearps, A., Greengrass, V., Hoy, J.F., Crowe, S.M., 2009, An HIV-1 integrase genotype assay for the detection of drug resistance mutations, Sexual Health [P], vol 6, issue 4, CSIRO Publishing, Australia, pp. 305-309.

Greengrass, V., Lohman, B., Morris, L., Plate, M., Steele, P.M., Walson, J., Crowe, S.M., 2009, Assessment of the low-cost cavidi exa vir load assay for monitoring HIV viral load in pediatric and adult patients, Jaids-Journal Of Acquired Immune Deficiency Synd..., vol 52, issue 3, Lippincott Williams & Wilkins, United States, pp. 387-390.

van Rooijen, L., Greengrass, V., Morris, L.M., Plate, M., Gouillou, M., Tachedjian, G., Sluis-Cremer, N., Hearps, A., Crowe, S.M., 2009, Effect of reverse transcriptase inhibitors and mutations on the low-cost Cavidi reverse transcriptase viral load assay, Jaids-Journal Of Acquired Immune Deficiency Synd..., vol 52, issue 4, Lippincott Williams & Wilkins, USA, pp. 527-529.

Westhorpe, C.L., Zhou, J., Webster, N., Kalionis, B., Lewin, S.R., Jaworowski, A., Muller, W., Crowe, S.M., 2009, Effects of HIV-1 infection in vitro on transendothelial migration by monocytes and monocyte-derived macrophages, Journal of Leukocyte Biology [P], vol 85, issue 6, Federation of American Societies for Experimental Biology, United States, pp. 1027-1035.

Jaworowski, A., Cheng, W., Westhorpe, C.L., Abendroth, A., Crowe, S.M., Slobedman, B., 2009, Enhanced monocyte Fc phagocytosis by a homologue of interleukin-10 encoded by human cytomegalovirus, Virology [P], vol 391, issue 1, Academic Press, United States, pp. 20-24.

Greengrass, V., Plate, M., Steele, P.M., Denholm, J., Cherry, C., Morris, L.M., Hearps, A., Crowe, S.M., 2009, Evaluation of the Cavidi ExaVir Load assay (version 3) for plasma human immunodeficiency virus type 1 load monitoring, Journal Of Clinical Microbiology [P], vol 47, issue 9, American Society of Microbiology, United States, pp. 3011-3013.

Plate, M., Louzao, R., Steele, P.M., Greengrass, V., Morris, L.M., Lewis, J., Barnett, D., Warrino, D., Hearps, A., Denny, T., Crowe, S.M., 2009, Evaluation of the blood stabilizers TransFixTM and Cyto-Chex(R) BCT for low-cost CD4 T-cell methodologies, Viral Immunology [P], vol 22, issue 5, Mary Ann Liebert, Inc. Publishers, United States, pp. 329-332.

Ryan, C.E., Kama, M., Darcy, A., Aleksic, E., Mirza, T., Chaudhary, A., Oelrichs, R., Rogers, G.D., Crowe, S.M., 2009, HIV type 1 in Fiji is caused by subtypes C and B, AIDS Research and Human Retroviruses [P], vol 25, issue 12, Mary Ann Liebert, Inc. Publishers, United States, pp. 1355-1358.

Brew, B.J., Crowe, S.M., Landay, A., Cysique, L.A., Guillemin, G.A., 2009, Neurodegeneration and Ageing in the HAART Era, Journal Of Neuroimmune Pharmacology [P], vol 4, issue 2, Springer New York LLC, United States, pp. 163-174.

Audsley, J.M., Arrifin, N., Yuen, L., Ayres, A., Crowe, S.M., Bartholomeusz, A., Locarnini, S.A., Mijch, A.M., Lewin, S.R., Sasadeusz, J., 2009, Prolonged use of tenofovir in HIV/hepatitis B virus (HBV)-coinfected individuals does not lead to HBV polymerase mutations and is associated with persistence of lamivudine HBV polymerase mutations, HIV Medicine [E], vol 10, issue 4, Wiley-Blackwell Publishing Ltd, United Kingdom, pp. 229-235.

Stevens, W., Gelman, R., Glencross, D.K., Scott, L.E., Crowe, S.M., Spira, T., 2008, Evaluating new CD4 enumeration technologies for resource-constrained countries, Nature Reviews Microbiology, vol 6, issue 11s, Macmillian Publishing Ltd, UK, pp. s29-s37.

Owe-Young, R., Webster, N.L., Mukhtar, M., Pomerantz, R.J., Smythe, G., Walker, D.W., Armati, P.J., Crowe, S.M., Brew, B.J., 2008, Kynurenine pathway metabolism in human blood-brain-barrier cells: implications for immune tolerance & neurotoxicity, Journal of Neurochemistry, vol 105, issue 4, Wiley-Blackwell Publishing Ltd, UK, pp. 1346-1357.

Jaworowski, A., Kamwendo, D.D., Ellery, P., Sonza, S., Mwapasa, V., Tadesse, E., Molyneux, M.E., Rogerson, S.J., Meshnick, S.R., Crowe, S.M., 2007, CD16+ monocyte subset preferentially harbors HIV-1 and is expanded in pregnant Malawian women with Plasmodium falciparum malaria and HIV-1 infection, Journal Of Infectious Diseases [P], vol 196, issue 1, Oxford University Press Inc., USA, pp. 38-42.

Gorry, P.R., Dunfee, R., Mefford, M.E., Kunstman, K., Morgan, T., Moore, J.P., Mascola, J.R., Agopian, K., Holm, G.H., Mehle, A., Taylor, J., Farzan, M., Wang, H., Ellery, P., Willey, S., Clapham, P.R., Wolinsky, S.M., Crowe, S.M., Gabuzda, D., 2007, Changes in the V3 region of gp120 contribute to unusually broad coreceptor usage of an HIV-1 isolate from a CCR5 delta32 heterozygote, Virology [P], vol 362, issue 1, Academic Press, USA, pp. 163-178.

Gorry, P.R., McPhee, D.A., Verity, E.E., Dyer, W.B., Wesselingh, S.L., Learmont J., Sullivan, J.S., Roche, M., Zaunders, J.J., Gabuzda, D., Crowe, S.M., Mills, J., Lewin, S.R., Brew, B.J., Cunningham, A.L., Churchill, M., 2007, Pathogenicity and immunogenicity of attenuated, nef-deleted HIV-1 strains in vivo, Retrovirology, vol 4, Biomed Central Ltd, England, pp. 1-13.

Berzsenyi, M.D., Bowden, D.S., Kelly, H.A., Watson, K.M., Mijch, A.M., Hammond, R.A., Crowe, S.M., Roberts, S.K., 2007, Reduction in hepatitis C-related liver disease associated with GB virus C in human immunodeficiency virus coinfection, Gastroenterology [P], vol 133, issue 6, WB Saunders Co, USA, pp. 1821-1830.

Ellery, P., Tippett, E.D., Chiu, Y., Paukovics, G., Cameron, P.U., Solomon, A., Lewin, S.R., Gorry, P.R., Jaworowski, A., Greene, W.C., Sonza, S., Crowe, S.M., 2007, The CD16+ monocyte subset is more permissive to infection and preferentially harbors HIV-1 in vivo, The Journal of Immunology, vol 178, issue 10, American Association of Immunologists, USA, pp. 6581-6589.

Ryan, C.E., Gare, J., Crowe, S.M., Wilson, K., Reeder, J.C., Oelrichs, R.B., 2007, The heterosexual HIV type 1 epidemic in Papua New Guinea is dominated by subtype C, AIDS Research and Human Retroviruses [P], vol 23, issue 7, Mary Ann Liebert, Inc. Publishers, USA, pp. 941-944.

Leeansyah, E., Wines, B.D., Crowe, S.M., Jaworowski, A., 2007, The mechanism underlying defective Fcgamma receptor-mediated phagocytosis by HIV-1-infected human monocyte-derived macrophages, Journal of Immunology, vol 178, issue 2, American Association of Immunologists, USA, pp. 1096-1104.

Hales, G., Birch, C.J., Crowe, S.M., Workman, C., Hoy, J.F., Law, M., Kelleher, A.D., Lincoln, D., Emery, S., 2006, A randomised trial comparing genotypic and virtual phenotypic interpretation of HIV drug resistance: the CREST study, PLoS Clinical Trials, vol 1, issue 3 (e18), Public Library of Science, USA, pp. 1-9.

Balakrishnan, P., Solomon, S., Mohanankrishnan, J., Cecelia, A.J., Kumarasamy, N., Murugavel, K.G., Venkatakrishnan, B., Solomon, S.S., Crowe, S.M., Ganesh, A.K., Thyagarajan, S.P., Flanigan, T., Mayer, K.H., 2006, A reliable and inexpensive EasyCD4 assay for monitoring HIV-infected individuals in resource-limited settings, Jaids-Journal Of Acquired Immune Deficiency Synd..., vol 43, issue 1, Lippincott Williams & Wilkins, USA, pp. 23-26.

Azzam, R., Lal, L., Goh, S., Kedzierska, K., Jaworowski, A., Naim, E., Cherry, C., Wesselingh, S.L., Mills, J., Crowe, S.M., 2006, Adverse effects of antiretroviral drugs on HIV-1-infected and -uninfected human monocyte-derived macrophages, Journal of Acquired Immune Deficiency Syndrome, vol 42, issue 1, Lippincott Williams & Wilkins, Philadelphia USA, pp. 19-28.

Truett, A.A., Crum, N.F., Crowe, S.M., 2006, Cyto-Chex BCT, not Cyto-Chex, should be used for preservation of CD4 cell counts, Jaids-Journal Of Acquired Immune Deficiency Syndromes [P], vol 43, issue 4, Lipponcott Williams & Wilkins, USA, pp. 503-504.

Truett, A.A., Letizia, A., Malyangu, E., Sinyangwe, F., Morales, B.N., Crum, N.F., Crowe, S.M., 2006, Efficacy of Cyto-Chex blood preservative for delayed manual CD4 testing using Dynal T4 Quant CD4 test among HIV-infected persons in Zambia, Jaids-Journal Of Acquired Immune Deficiency Syndromes [P], vol 41, issue 2, Lippincott Williams & Wilkins, USA, pp. 168-174.

Azzam, R., Kedzierska, K., Leeansyah, E., Chan, H., Doischer, D., Gorry, P.R., Cunningham, A.L., Crowe, S.M., Jaworowski, A., 2006, Impaired complement-mediated phagocytosis by HIV type-1-infected human monocyte-derived macrophages involves a cAMP-dependent mechanism, AIDS Research and Human Retroviruses, vol 22, issue 7, Mary Ann Liebert, Inc. Publishers, USA, pp. 619-629.

Webster, N.L., Crowe, S.M., 2006, Matrix metalloproteinases, their production by monocytes and macrophages and their potential role in HIV-related diseases, Journal of Leukocyte Biology, vol 80, issue 5, Federation of American Societies for Experimental Biology, USA, pp. 1052-1066.

Jaworowski, A., Ellery, P., Maslin, C.L.V., Naim, E., Heinlein, A.C., Ryan, C.E., Paukovics, G., Hocking, J., Sonza, S., Crowe, S.M., 2006, Normal CD16 expression and phagocytosis of Mycobacterium avium complex by monocytes from a current cohort of HIV-1-infected patients, The Journal of Infectious Diseases, vol 193, issue 5, University of Chicago Press, USA, pp. 693-697.

Webster, N.L., Kedzierska, K., Azzam, R., Paukovics, G., Wilson, J.W., Crowe, S.M., Jaworowski, A., 2006, Phagocytosis stimulates mobilization and shedding of intracellular CD16A in human monocytes and macrophages: inhibition by HIV-1 infection, Journal of Leukocyte Biology, vol 79, issue 2, Federation of American Societies for Experimental Biology, USA, pp. 294-302.

Shehu-Xhilaga, M., Tachedjian, G., Crowe, S.M., Kedzierska, K., 2005, Antiretroviral compounds: mechanisms underlying failure of HAART to eradicate HIV-1, Current Medicinal Chemistry, vol 12, issue 15, Bentham Science Publishers Ltd., The Netherlands, pp. 1705-1719.

Jennings, C., Fiscus, S.A., Crowe, S.M., Danilovic, A.D., Morack, R.J., Scianna, S., Cachafeiro, A., Brambilla, D.J., Schupbach, J., Stevens, W., Respess, R., Varnier, O.E., Corrigan, G.E., Gronowitz, J.S., Ussery, M.A., Bremer, J.W., 2005, Comparison of two human immunodeficiency virus (HIV) RNA surrogate assays to the standard HIV RNA assay, Journal Of Clinical Microbiology [P], vol 43, issue 12, American Society for Microbiology, USA, pp. 5950-5956.

Crowe, S.M., 2005, Effect of HIV-1 infection of monocytes and monocyte-derived macrophages on transenclothelial migration in vitro, Tissue Antigens, vol 66, issue 5, Blackwell Publishing, Oxford England, pp. 489-490.

Greengrass, V.L., Turnbull, S.P., Hocking, J., Dunne, A.L., Tachedjian, G., Corrigan, G.E., Crowe, S.M., 2005, Evaluation of a low cost reverse transcriptase assay for plasma HIV-1 viral load monitoring, Current HIV Research, vol 3, issue 2, Bentham Science Publishers Ltd, The Netherlands, pp. 183-190.

Crowe, S.M., Ho, D.D., Marriott, D., Gorry, P.R., Sullivan, J.S., Learmont J., Mills, J., 2005, In vivo replication kinetics of a nef-deleted strain of HIV-1 [2], AIDS, vol 19, issue 8, Lippincott Williams and Wilkins, USA, pp. 842-843.

Gorry, P.R., Churchill, M., Crowe, S.M., Cunningham, A.L., Gabuzda, D., 2005, Pathogenesis of macrophage tropic HIV-1, Current HIV Research, vol 3, issue 1, Bentham Science Publishers Ltd, The Netherlands, pp. 53-60.

Duncan, A.J., Crowe, S.M., 2005, Providing HIV education for health professionals in India, Journal of Pharmacy Practice and Research, vol 35, issue 4, Society of Hospital Pharmacists of Australia, Australia, pp. 291-292.

Emery, S., Workman, C., Puls, R.L., Bloch, M., Baker, D., Bodsworth, N., Anderson, J., Crowe, S.M., French, M.A.H., Hoy, J., Aichelburg, A., Ward, L.D., Boyle, D.B., Law, M.G., Kelleher, A.D., Cooper, D.A., 2005, Randomized, placebo-controlled, phase I/IIa evaluation of the safety and immunogenicity of fowlpox virus expressing HIV gag-pol and interferon-gamma in HIV-1 infected subjects., Human Vaccines, vol 1, issue 6, Landes Bioscience, USA, pp. 232-238.

Maslin, C.L.V., Kedzierska, K., Webster, N.L., Muller, W.A., Crowe, S.M., 2005, Transendothelial migration of monocytes: the underlying molecular mechanisms and consequences of HIV-1 infection, Current HIV Research, vol 3, issue 4, Bentham Science Publishers Ltd, The Netherlands, pp. 303-317.

Gray, L., Sterjovski J., Churchill, M., Ellery, P., Nasr, N., Lewin, S.R., Crowe, S.M., Wesselingh, S.L., Cunningham, A.L., Gorry, P.R., 2005, Uncoupling coreceptor usage of human immunodeficiency virus type 1 (HIV-1) from macrophage tropism reveals biological properties of CCR5-restricted HIV-1 isolates from patients with acquired immunodeficiency syndrome, Virology, vol 337, issue 2, Academic Press, USA, pp. 384-398.

Kedzierska, K., Maslin, C.L.V., Crowe, S.M., 2004, Cells of the Macrophage Lineage and their Role in the Pathogenesis of HIV-1 Infection: An Update, Medicinal Chemistry Reviews Online, vol 1, issue 3, Bantham Science Publishers Ltd, Netherland, pp. 351-361.

Kedzierska, K., Paukovics, G., Handley, A., Hewish, M., Hocking, J., Cameron, P.U., Crowe, S.M., 2004, Interferon-gamma Therapy Activates Human Monocytes for Enhanced Phagocytosis of Mycobacterium avium Complex in HIV-Infected Individuals, HIV Clinical Trials, vol 5, issue 2, Thomas Land Publishers, Inc, USA, pp. 80-85.

Churchill, M., Sterjovski J., Gray L., Cowley D., Chatfield C., Learmont J., Sullivan, J.S., Crowe, S.M., Mills, J., Brew, B., Wesselingh, S.L., McPhee, D., Gorry, P.R., 2004, Longitudinal Analysis of nef/Long Terminal Repeat-Deleted HIV-1 in Blood and Cerebrospinal Fluid of a Long-Term Survivor Who Developed HIV-Associated Dementia, The Journal of Infectious Disease, vol 190, Infectious Diseases Society of America, USA, pp. 2181-2186.

Campbell, S., Gaus, K., Bittman, R., Jessup, W., Crowe, S.M., Mak, J., 2004, The Raft-Promoting Property of Virion-Associated Cholesterol, but Not the Presence of Virion-Associated Brij 98 Rafts, Is a Determinant of Human Immunodeficiency Virus Type 1 Infectivity, Journal of Virology, vol 78, issue 19, American Society of Microbiology, USA, pp. 10556-10565.

Ryan, C.E., Elliott, J.H., Middleton, T., Mijch, A.M., Street, A.C., Hellard, M., Crofts, N., Crowe, S.M., Oelrichs, R.B., 2004, The molecular epidemiology of HIV type 1 among Vietnamese Australian injecting drug users in Melbourne, Australia, AIDS Research and Human Retroviruses [P], vol 20, issue 12, Mary Ann Liebert, Inc. Publishers, USA, pp. 1364-1367.

Dunne, A.L., Mitchell, F.M., Allen, K.M., Baker, H., Garland, S.M., Clarke, G., Mijch, A.M., Crowe, S.M., 2003, Analysis of HIV-1 viral load in seminal plasma samples, Journal of Clinical Virology [P], vol 26, issue 2, Elsevier BV, The Netherlands, pp. 239-245.

Kedzierska, K., Azzam, R., Ellery, P., Mak, J., Jaworowski, A., Crowe, S.M., 2003, Defective phagocytosis by human monocyte/macrophages following HIV-1 infection: underlying mechanisms and modulation by adjunctive cytokine therapy, Journal of Clinical Virology, vol 26, issue 2, Elsevier Science BV, Amsterdam The Netherlands, pp. 247-263.

Warby, T., Crowe, S.M., Jaworowski, A., 2003, Human immunodeficiency virus type 1 infection inhibits granulocyte-macrophage colony-stimulating factor-induced activation of STAT5A in human monocyte-derived macrophages, Journal Of Virology [P], vol 77, issue 23, American Society for Microbiology, USA, pp. 12630-12638.

Solomon, A., Cameron, P.U., Bailey, M.J., Dunne, A.L., Crowe, S.M., Hoy, J.F., Lewin, S.R., 2003, Immunological and virological failure after antiretroviral therapy is associated with enhanced peripheral and thymic pathogenicity, The Journal of Infectious Diseases, vol 187, issue 12, University of Chicago Press, Chicago USA, pp. 1915-1923.

Birch, C., Middleton, T., Hales, G., Cooper, D.A., Law, M., Crowe, S.M., Hoy, J.F., Emery, S., 2003, Limited evolution of HIV antiretroviral drug resistance-associated mutations during the performance of drug resistance testing, JAIDS Journal of Acquired Immune Deficiency Syndromes, vol 32, issue 1, Lippincott Williams & Wilkins, Philadelphia USA, pp. 57-61.

Crowe, S.M., Turnbull, S., Oelrichs, R., Dunne, A.L., 2003, Monitoring of human immunodeficiency virus infection in resource-constrained countries, Clinical Infectious Diseases, vol 37, issue Supp 1, University of Chicago Press, Chicago USA, pp. 25-35.

Kedzierska, K., Churchill, M., Maslin, C.L.V., Azzam, R., Ellery, P., Chan, H., Wilson, J.W., Deacon, N.J., Jaworowski, A., Crowe, S.M., 2003, Phagocytic efficiency of monocytes and macrophages obtained from Sydney Blood Bank Cohort members infected with an attenuated strain of HIV-1, Journal of Acquired Immune Deficiency Syndromes, vol 34, issue 5, Lippincott Williams & Wilkins, Philadelphia USA, pp. 445-453.

Cooley, L., Ayres, A., Bartholomeusz, A., Lewin, S.R., Crowe, S.M., Mijch, A.M., Locarnini, S., Sasadeusz, J., 2003, Prevalence and characterization of lamivudine-resistant hepatitis B virus mutations in HIV-HBV co-infected individuals, AIDS [P], vol 17, issue 11, Lippincott Williams & Wilkins, USA, pp. 1649-1657.

Crowe, S.M., Zhu, T., Muller, W.A., 2003, The contribution of monocyte infection and trafficking to viral persistence, and maintenance of the viral reservoir in HIV infection, Journal of Leukocyte Biology [P], vol 74, issue 5, Federation of American Societies for Experimental Biology, USA, pp. 635-641.

Hill, M.K., Shehu-Xhilaga, M., Campbell, S.M., Poumbourios, P., Crowe, S.M., Mak, J., 2003, The dimer initiation sequence stem-loop of human immunodeficiency virus type 1 is dispensable for viral replication in peripheral blood mononuclear cells, Journal of Virology, vol 77, issue 15, American Society for Microbiology, Washington USA, pp. 8329-8335.

Baker, H.W., Mijch, A.M., Garland, S.M., Crowe, S.M., Dunne, M., Edgar, D., Clarke, G., Foster, P., Blood, J., 2003, Use of assisted reproductive technology to reduce the risk of transmission of HIV in discordant couples wishing to have their own children where the male partner is seropositive with an undetectable viral load, Journal Of Medical Ethics [P], vol 29, issue 6, BMJ Group, UK, pp. 315-320.

Kedzierska, K., Ellery, P., Mak, J., Lewin, S.R., Crowe, S., Jaworowski, A., 2002, HIV-1Down-Modulates Signaling Chain of Fcin Human Macrophages: A Possible Mechanism for Inhibition of Phagocytosis, Journal of Immunology, vol 168, issue 6, American Association of Immunologists, Bethesda MD USA, pp. 2895-2903.

Campbell, S.M., Crowe, S.M., Mak, J., 2002, Virion-associated cholesterol is critical for the maintenance of HIV-1 structure and infectivity, AIDS, vol 16, issue 17, Lippincott Williams & Wilkins, Philadelphia USA, pp. 2253-2261.

Dunne, A.L., Mitchell, F.M., Coberly, S.K., Hellmann, N.S., Hoy, J., Mijch, A.M., Petropoulos, C.J., Mills, J., Crowe, S., 2001, Comparison of genotyping and phenotyping methods for determining susceptibility of HIV-1 to antiretroviral drugs, AIDS, vol 15, issue 12, Lippincott Willimas & Wilkins, Philadelphia Pennsylvania USA, pp. 1471-1475.

Mijch, A.M., Hoy, J., Watson, K., Dunne, A.L., Crowe, S., Wesselingh, S.L., 2001, Does plasma HIV RNA predict outcome in a cohort of treated HIV-infected inidividuals followed over 3 years?, Journal of Clinical Virology, vol 22, issue 3, Elsevier Science BV, Amsterdam Netherlands, pp. 271-278.

Kedzierska, K., Vardaxis, N.J., Jaworowski, A., Crowe, S., 2001, FcgR-mediated phagocytosis by human macrophages involves Hck, Syk, and Pyk2 and is augmented by GM-CSF, Journal of Leukocyte Biology, vol 70, Federation of American Society of Experimental Biology, Bethesda MD USA, pp. 322-328.

Hill, M., Hooker, C., Harrich, D., Crowe, S., Mak, J., 2001, Gag-pol supplied in trans is efficiently packaged and supports viral function in human immunodeficiency virus type 1, Journal of Virology, vol 75, issue 15, American Society for Microbiology, Washington DC USA, pp. 6835-6840.

Campbell, S., Crowe, S., Mak, J., 2001, Lipid rafts and HIV-1: from viral entry to assembly of progeny virions, Journal of Clinical Virology, vol 22, Elsevier Science BV, Amsterdam Netherlands, pp. 217-227.

Shehu-Xhilaga, M., Crowe, S., Mak, J., 2001, Maintenance of the Gag/Gag-Pol ratio is important for human immunodeficiency virus type 1 RNA dimerization and viral infectivity, Journal of Virology, vol 75, issue 4, American Society of Microbiology, Washington DC USA, pp. 1834-1841.

Kedzierska, K., Mak, J., Jaworowski, A., Greenway, A., Violo, A., Chan, H., Hocking, J., Purcell, D., Sullivan, J.S., Mills, J., Crowe, S., 2001, Nef-deleted HIV-1 inhibits phagocytosis by monocyte-derived macrophages in vitro but not by peripheral blood monocytes in vivo, AIDS, vol 15, issue 8, Lippincott Williams & Wilkins, Philadelphia USA, pp. 945-955.

Shehu-Xhilaga, M., Kraeusslich, H.G., Pettit, S., Swanstrom, R., Lee, J.Y., Marshall, J.A., Crowe, S., Mak, J., 2001, Proteolytic processing of the P2/Nucleocapsid cleavage site is critical for human immunodeficiency virus type 1 RNA Dimer maturation, Journal of Virology, vol 75, issue 19, American Society for Microbiology, Washington DC USA, pp. 9156-9164.

Chan, H., Kedzierska, K., O'Mullane, J., Crowe, S., Jaworowski, A., 2001, Quantifying complement-mediated phagocytosis by human monocyte-derived macrophages, Immunology and Cell Biology, vol 79, Blackwell Science Asia, Carlton Vic Australia, pp. 429-435.

Grants

Title:

BD Biosciences FACSAria flow cytometer.

Investigators:

Rolland, J, Lewin, S, O'Hehir, R, Wesselingh, S, Jackson, S, Toh, B, Crowe, S

Funding:

(2005 - 2009). National Health & Medical Research Council (NHMRC).

Title:

Pathogenesis of persistent human virus infections of global significance: ID 299907.

Investigators:

Cunningham, A, Brew, B, Crowe, S, Lewin, S, Slobedman, B, Wesselingh, S

Funding:

(2005 - 2009). National Health & Medical Research Council (NHMRC).

Postgraduate Research Supervisions

Current Supervision

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Antiretroviral therapy in Papua New Guinea: biological and social factors contributing to treatment failure and drug resistance.

Supervisors:

Crowe, S (Main), Hearps, A (Associate).

Completed Supervision

Student:

Azzam, R.

Program of Study:

Mechanisms underlying defective complement-mediated phagocytosis by HIV-1 infected human monocyte-derived macrophages. (PHD) 2007.

Supervisors:

Crowe, S (Joint-Co), Jaworowski, A (Joint).

Student:

Ellery, P.

Program of Study:

The role of the minor CD16+ monocyte subset in the pathogenesis of human immunodeficiency virus type 1 infection. (PHD) 2006.

Supervisors:

Crowe, S (Joint-Co), Sonza, S (Joint).

Student:

Kedzierska, K.

Program of Study:

MECHANISMS UNDERLYING DEFECTIVE PHAGOCYTOSIS BY HUMAN MONOCYTES AND MACROPHAGES FOLLOWING HIV-1 INFECTION.. (PHD) 2001.

Supervisors:

Crowe, S (Main), Jaworowski, A (Associate).

Student:

Leeansyah, E.

Program of Study:

The effect of HIV-1 infection on Fc^gamma receptor signalling and function. (PHD) 2008.

Supervisors:

Jaworowski, A (Main), Crowe, S (Associate).

Student:

Lichtfuss, G.

Program of Study:

HiACT - HIV immune activation and signals through ITAM. (PHD) 2011.

Supervisors:

Crowe, S (Main), Jaworowski, A (Associate), Lewin, S (Associate).

Student:

Ryan, C.

Program of Study:

Monitoring the HIV-1 epidemic in Indonesia and the Pacific region. (PHD) 2009.

Supervisors:

Crowe, S (Main).

Student:

Tippett, E.

Program of Study:

The role of monocyte subsets in HIV-1 pathogenesis and their contribution to the viral reservoir. (PHD) 2011.

Supervisors:

Crowe, S (Main), Cameron, P (Associate), Lewin, S (Associate).

Student:

Westhorpe, C.

Program of Study:

Effects of HIV-1 infection in vitro on transendothelial migration by cells of the macrophage lineage. (PHD) 2008.

Supervisors:

Crowe, S (Main), Webster, N (Associate).

Student:

Xhilaga, M.

Program of Study:

PROTEOLYTIC PROCESSING OF GAG AND GAGPROPOL PRECURSOR PROTEINSOF HIV-1, GENOMIC RNA REARRANGEMENTS AND VIRION CORE FORMATION ARE INTERRELATED. (PHD) 2001.

Supervisors:

Crowe, S (Joint-Co), Mak ((Double Id)), J (Joint).