Dr Tony Velkov - Researcher Profile

Biography

Research Fellow
Career development Fellow Industry Level 1
Faculty of Pharmacy and Pharmaceutical Sciences

Drug Delivery Disposition and Dynamics
Monash Institute of Pharmaceutical Sciences

 

 

 

 

 

 

Research & Supervision Interests

drug-protein interactions, isothermal titration calorimetry, Surface Plasmon Resonance, fluorescence structural-biology of viruses anti-infective drug discovery and lead optimisation to treat infections caused by medically important viruses and bacteria bioinformatics.

Qualifications

BACHELOR OF SCIENCE DEGREE

Institution: Monash University

Year awarded: 2006

PHD BIOCHEMISTRY

Institution: Monash University

Year awarded: 2006

RESEARCH SUPERVISOR TRAINING WORKSHOP

Institution: Monash University

Year awarded: 2006

Publications

Journal Articles

Baker, M.A., Hetherington, L., Weinberg, A.S., Naumovski, N., Velkov, T., Pelzing, M., Dolman, S., Condina, M., Aitken, R.J., 2012, Analysis of phosphopeptide changes as spermatozoa acquire functional competence in the epididymis demonstrates changes in the post-translational modification of izumo1, Journal Of Proteome Research [P], vol 11, issue 11, American Chemical Society, Washington DC, USA, pp. 5252-5264.

Huang, J.X., Cooper, M.A., Baker, M.A., Azad, M.A.K., Nation, R.L., Li, J., Velkov, T., 2012, Drug-binding energetics of human a-1-acid glycoprotein assessed by isothermal titration calorimetry and molecular docking simulations, Journal Of Molecular Recognition [P], vol 25, issue 12, Wiley-Blackwell, Malden, Massachusetts USA, pp. 642-656.

Huang, J.X., Azad, M.A.K., Yuriev, E., Baker, M.A., Nation, R.L., Li, J., Cooper, M.A., Velkov, T., 2012, Molecular characterization of lipopolysaccharide binding to human alpha-1-acid glycoprotein, Journal of Lipids [E], vol 2012, Hindawi Publishing Corporation, USA, pp. 1-15.

Azad, M.A.K., Huang, J.X., Cooper, M.A., Roberts, K.D., Thompson, P., Nation, R.L., Li, J., Velkov, T., 2012, Structure-activity relationships for the binding of polymyxins with human alpha-1-acid glycoprotein, Biochemical Pharmacology [P], vol 84, issue 3, Pergamon-Elsevier Science Ltd, Oxford, England, pp. 278-291.

Deris, Z.Z., Yu, H., Davis, K.E., Soon, R.L., Jacob, J., Ku, K.U.C., Poudyal, A., Bergen, P.J., Tsuji, B., Bulitta, J.B., Forrest, A., Paterson, D.L., Velkov, T., Li, J., Nation, R.L., 2012, The combination of colistin and doripenem is synergistic against Klebsiella pneumoniae at multiple inocula and suppresses colistin resistance in an in vitro pharmacokinetic/pharmacodynamic model, Antimicrobial Agents And Chemotherapy [P], vol 56, issue 10, American Society for Microbiology, Washington DC, USA, pp. 5103-5112.

Velkov, T., Thompson, P., El-Kabbani, O., Lindh, F., Stambas, J., Rockman, S., 2011, A gel-capture assay for characterizing the sialyl-glycan selectivity of influenza viruses, Acta Virologica [P], vol 55, issue 2, AEPRESS SRO, SLOVAKIA, pp. 131-137.

Velkov, T., Horne, J., Scanlon, M., Capuano, B., Yuriev, E., Lawen, A., 2011, Characterization of the N-methyltransferase activities of the multifunctional polypeptide cyclosporin synthetase, Chemistry and Biology [P], vol 18, issue 4, Cell Press, USA, pp. 464-475.

Soon, R., Velkov, T., Chiu, F., Thompson, P., Kancharla, R., Roberts, K., Larson, I., Nation, R., Li, J., 2011, Design, synthesis, and evaluation of a new fluorescent probe for measuring polymyxin-lipopolysaccharide binding interactions, Analytical Biochemistry [P], vol 409, issue 2, Academic Press, United States, pp. 273-283.

Henderson, L.C., Li, J., Nation, R.L., Velkov, T., Pfeffer, F.M., 2010, Developing an anion host for lipid A binding and antibacterial activity, Chemical Communications [P], vol 46, Royal Society of Chemistry, United Kingdom, pp. 3197-3199.

Velkov, T., Rimmer, K., Headey, S., 2010, Ligand-enhanced expression and in-cell assay of human peroxisome proliferator-activated receptor alpha ligand binding domain, Protein Expression And Purification [P], vol 70, issue 2, Academic Press, United States, pp. 260-269.

Wallace, S.J., Li, J., Nation, R.L., Prankerd, R.J., Velkov, T., Boyd, B.J., 2010, Self-assembly behavior of colistin and its prodrug colistin methanesulfonate: implications for solution stability and solubilization, Journal of Physical Chemistry B [P], vol 114, American Chemical Society, USA, pp. 4836-4840.

Velkov, T., Thompson, P., Nation, R.L., Li, J., 2010, Structure-Activity Relationships of Polymyxin Antibiotics, Journal of Medicinal Chemistry [P], vol 53, American Chemical Society, USA, pp. 1898-1916.

Velkov, T., Hughes, M.L.R., Horne, H.J., Simpson, J.S., Porter, C.J., Scanlon, M., 2009, Characterization of lipophilic drug binding to rat intestinal fatty acid binding protein, Molecular And Cellular Biochemistry [P], vol 326, issue 1-2, Springer New York LLC, USA, pp. 87-95.

Chuang, S., Velkov, T., Horne, H.J., Wielens, J., Chalmers, D.K., Porter, C.J., Scanlon, M., 2009, Probing the fibrate binding specificity of rat liver fatty acid binding protein, Journal of Medicinal Chemistry [P], vol 52, issue 17, American Chemical Society, USA, pp. 5344-5355.

velkov, T., Lim, M.L., Capuano, B., Prankerd, R.J., 2008, A protocol for the combined sub-fractionation and delipidation of lipid binding proteins using hydrophobic interaction chromatography, JOURNAL OF CHROMATOGRAPHY. B, ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, vol 867, issue 2, ELSEVIER SCIENCE BV, NETHERLANDS, pp. 238-246.

Chuang, S., Velkov, T., Horne, J., Porter, C.J., Scanlon, M., 2008, Characterization of the drug binding specificity of rat liver fatty acid binding protein, Journal of Medicinal Chemistry, vol 51, issue 13, American Chemical Society, USA, pp. 3755-3764.

Velkov, T., Jones, A., Lim, M.L., 2008, Ni2+-Based Immobilized Metal Ion Affinity Chromatography of Lactose Operon Repressor Protein from Escherichia Coli, Preparative Biochemistry & Biotechnology [P], vol 38, issue 4, Taylor and Francis, Inc., USA, pp. 422-441.

Vivian, J., Scoullar, J., Robertson, A.L., Bottomley, S.P., Horne, H.J., Chin, K.Y., Wielens, J., Thompson, P., Velkov, T., Piek, S., Byres, E., Beddoe, T.C., Wilce, M.C., Kahler, C.M., Rossjohn, J., Scanlon, M., 2008, Structural and biochemical characterization of the oxidoreductase NmDsbA3 from Neisseria meningitidis, The Journal of Biological Chemistry, vol 283, issue 47, American Society for Biochemistry and Molecular Biology Inc., United States, pp. 32452-32461.

Velkov, T., Horne, H.J., Laguerre, A.K., Jones, E.D., Scanlon, M., Porter, C.J., 2007, Examination of the role of intestinal fatty acid-binding protein in drug absorption using a parallel artificial membrane permeability assay, Chemistry & Biology, vol 14, issue 4, Cell Press, USA, pp. 453-465.

Jazayeri, J., De Weerd, N.A., Raye, W.S., Velkov, T., Santos, L.L., Taylor, D., Carroll, G.J., 2007, Generation of mutant leukaemia inhibitory factor (LIF)-IgG heavy chain fusion proteins as bivalent antagonists of LIF, Journal of Immunological Methods, vol 323, issue 1, Elsevier Science BV, Netherlands, pp. 1-10.

Horne, H.J., d'Auvergne, E.J., Coles, M., Velkov, T., Chin, Y., Charman, W.N., Prankerd, R.J., Gooley, P., Scanlon, M., 2007, Probing the Flexibility of the DsbA Oxidoreductase from Vibrio cholerae-a 15N - 1H Heteronuclear NMR Relaxation Analysis of Oxidized and Reduced Forms of DsbA, Journal of Molecular Biology, vol 371, issue 3, Academic Press, England, pp. 703-716.

Velkov, T., Singaretnam, L.G., Lawen, A., 2006, An improved purification procedure for cyclosporin synthetase, Protein Expression and Purification, vol 45, issue 2, Academic Press, USA, pp. 275-287.

Velkov, T., Singaretnam, L.G., Lawen, A., 2006, An improved purification procedure for cyclosporin synthetase, Protein Expression and Purification, vol 45, issue 2, Elsevier Science, San Diego USA, pp. 275-287.

Velkov, T., Chuang, S., Prankerd, R.J., Sakellaris, H.H., Porter, C.J.H., Scanlon, M.J., 2005, An improved method for the purification of rat liver-type fatty acid binding protein from Escherichia coli, Protein Expression & Purification, vol 44, issue 1, Academic Press, USA, pp. 23-31.

Velkov, T., Chuang, S., Wielens, J., Sakellaris, H.H., Charman, W.N., Porter, C.J., Scanlon, M., 2005, The interaction of lipophilic drugs with intestinal fatty acid-binding protein, The Journal of Biological Chemistry, vol 280, issue 18, The American Society for Biochemistry and Molecular Biology, Inc., USA, pp. 17769-17776.

Velkov, T., Lawen, A., 2003, Mapping and molecular modeling of S-adenosyl-L-methionine binding site in N-methyltransferase domains of the multifunctional polypeptide cyclosporin synthetase, Journal of Biological Chemistry, vol 278, issue 2, American Society for Biochemistry and Molecular Biology, Inc, USA, pp. 1137-1148.

Velkov, T., Lawen, A., 2003, Non-ribosomal peptide synthetases as technological platforms for the synthesis of highly modified peptide bioeffectors - cyclosporin synthetase as a complex example, Biotechnology Annual Review, vol 9, Elsevier BV, The Netherlands, pp. 151-197.

Velkov, T., Lawen, A., 2003, Photoaffinity labeling of the N-methyltransferase domains of cyclosporin synthetase, Photochemistry and Photobiology, vol 77, issue 2, Royal Society of Chemistry, Cambridge England, pp. 129-137.

Velkov, T., Lawen, A., 2002, Mapping and molecular modeling of S-adenosyl-L-methionine binding sites in N-methyltransferase domains of the multifunctional polypeptide cyclosporin synthetase, Journal of Biological Chemistry, vol 278, issue 2, American Society of Biochemistry and Molecular Biology Inc., Bethesda MD USA, pp. 1137-1148.

Teaching Commitment

• Lecture in advanced spectroscopy and biomolecules in the Bachelor of Pharmaceutical Sciences

Grants

Title:

Binocular Stereo Microscope.

Investigators:

Scanlon, M, Velkov, T

Funding:

(2007 - 2011). Collier Charitable Fund.

Title:

Combating bacterial 'superbugs' by innovative dosing strategies that combine available antibiotics to prevent resistance.

Investigators:

Bulitta, J, Oliver, A, Landersdorfer, C, Velkov, T, Nation, R, Boyce, J, Kirkpatrick, C

Funding:

(2013 - 2017). National Health & Medical Research Council (NHMRC).

Title:

Rescuing the last-line therapy colistin against Gram-negative ‘superbugs’: increasing the therapeutic index by attenuation of nephrotoxicity.

Investigators:

Li, J, Hill, P, Nation, R, Velkov, T

Funding:

(2012 - 2016). National Health & Medical Research Council (NHMRC).

Title:

Small molecule inhibitors of apical bile acid transporter in the ileum as therapeutic agents for hypercholesterolemia.

Investigators:

Velkov, T

Funding:

(2011 - 2015). Helen Macpherson Smith Trust.

Title:

Targeting Superbugs: discovery and development of new broad-spectrum lipopeptide.

Investigators:

Li, J, Velkov, T, Nation, R, Thompson, P, Roberts, K, Dudley, M, Hecker, S, Lomovskaya, O, Griffith, D

Funding:

(2012 - 2016). National Institute of Health, US.

Title:

Targeting polymyxin-resistant Gram-negative 'superbugs': development of novel antimicrobial peptides.

Investigators:

Li, J, Velkov, T, Nation, R, Thompson, P

Funding:

(2010 - 2013). National Health & Medical Research Council (NHMRC).

(2011 - 2015). National Health & Medical Research Council (NHMRC).

Title:

UV-visible spectrophotometer and software.

Investigators:

El-Kabbani, O, Capuano, B, Chung, R, Velkov, T

Funding:

(2009 - 2012). Collier Charitable Fund.

Title:

The structure and receptor binding properties of the 2009 swine influenza pandemic Hemagglutinin.

Investigators:

El-Kabbani, O, Velkov, T

Funding:

(2009 - 2013). National Health & Medical Research Council (NHMRC).

Title:

NHMRC Career Development Award - Level 1 - Industry.

Investigators:

Velkov, T

Funding:

(2011 - 2015). National Health & Medical Research Council (NHMRC).

Title:

Structural biology of mesothelin in the metastasis of asbestos-induced malignant pleural mesothelioma.

Investigators:

Velkov, T

Funding:

(2011 - 2015). Australian Lung Foundation.

Title:

An examination of the role of sterol carrier protein and ileal bile acid binding protein in drug absorption: ID 384300.

Investigators:

Velkov, T

Funding:

(2006 - 2009). National Health & Medical Research Council (NHMRC).