Dr Kylie Wagstaff - Researcher Profile

Biography

Kylie works in the Faculty of Medicine, Nursing & Health Sciences at Monash University as a Research Fellow.

Qualifications

DOCTOR OF PHILOSOPHY

Institution: Monash University

Year awarded: 2008

BACHELOR OF BIOMEDICAL SCIENCE

Institution: Monash University

Year awarded: 2003

BACHELOR OF BIOMEDICAL SCIENCE WITH HON

Institution: Monash University

Year awarded: 2003

Publications

Book Chapters

Wagstaff, K., Jans, D., 2010, Protein Transduction: Cell Penetrating Peptides and their Therapeutic Applications, in Frontiers in Medicinal Chemistry (5), eds Atta-ur-Rahman, AB Reitz, MI Choudhary, Bentham Science Publishers Ltd, The Netherlands, pp. 98-126.

Davies, R., Jans, D.A., Wagstaff, K.M., 2010, Use of fluorescence photobleaching techniques to measure the kinetics of intracellular transport, in Microscopy: Science, Technology, Applications and Education, eds A Mendez-Vilas and J Diaz, Formatex, Spain, pp. 756-763.

Journal Articles

Kuusisto, H.V., Wagstaff, K.M., Alvisi, G., Martino Roth, D., Jans, D.A., 2012, Global enhancement of nuclear localization-dependent nuclear transport in transformed cells, Faseb Journal [P], vol 26, issue 3, Federation of American Societies for Experimental Biology, USA, pp. 1181-1193.

Wagstaff, K.M., Sivakumaran, H., Heaton, S.M., Harrich, D., Jans, D.A., 2012, Ivermectin is a specific inhibitor of importin alpha/beta-mediated nuclear import able to inhibit replication of HIV-1 and dengue virus, Biochemical Journal [E], vol 443, issue 3, Portland Press Ltd, UK, pp. 851-856.

Caly, L., Wagstaff, K.M., Jans, D.A., 2012, Nuclear trafficking of proteins from RNA viruses: potential target for antivirals?, Antiviral Research [P], vol 95, issue 3, Elsevier BV, The Netherlands, pp. 202-206.

Wagstaff, K.M., Rawlinson, S.M., Hearps, A., Jans, D.A., 2011, An AlphaScreen®-based assay for high-throughput screening for specific inhibitors of nuclear import, Journal of Biomolecular Screening [P], vol 16, issue 2, Sage Publications, Inc., USA, pp. 192-200.

Newton, H.J., Pearson, J.S., Badea, L., Kelly, M., Lucas, M., Holloway, G., Wagstaff, K.M., Dunstone, M.A., Sloan, J., Whisstock, J.C., Kaper, J.B., Robins-Browne, R.M., Jans, D.A., Frankel, G., Phillips, A.D., Coulson, B.S., Hartland, E.L., 2010, The type III effectors NleE and NleB from enteropathogenic E. coli and OspZ from Shigella block nuclear translocation of NF-kappaB p65, Plos Pathogens [P], vol 6, issue 5 (e1000898), Public Library of Science, USA, pp. 1-16.

Wagstaff, K.M., Jans, D.A., 2009, Importins and beyond: Non-conventional nuclear transport mechanisms, Traffic [P], vol 10, issue 9, Wiley-Blackwell Munksgaard, Denmark, pp. 1188-1198.

Wagstaff, K.M., Jans, D.A., 2009, Nuclear drug delivery to target tumour cells, European Journal of Pharmacology [P], vol 625, issue 1-3, Elsevier BV, The Netherlands, pp. 174-180.

Wagstaff, K.M., Fan, J.Y., De Jesus, M., Tremethick, D.J., Jans, D.A., 2008, Efficient gene delivery using reconstituted chromatin enhanced for nuclear targeting, The FASEB Journal, vol 22, issue 7, Federation of American Societies for Experimental Biology, United States, pp. 2232-2242.

Kuusisto, H.V., Wagstaff, K.M., Alvisi, G., Jans, D.A., 2008, The C-terminus of apoptin represents a unique tumor cell-enhanced nuclear targeting module, International Journal of Cancer, vol 123, issue 12, John Wiley & Sons, Inc., United States, Switzerland, Germany, pp. 2965-2969.

Hearps, A.C., Wagstaff, K.M., Piller, S., Jans, D.A., 2008, The N-terminal basic domain of the HIV-1 matrix protein does not contain a conventional nuclear localization sequence but is required for DNA binding and protein self-association, Biochemistry, vol 47, issue 7, Americal Chemical Society, United States, pp. 2199-2210.

Jans, D.A., Wagstaff, K.M., 2007, Getting to the heart of a tumour cell, Pharma Focus Asia, vol 0, issue 5, Ochre Media, Hyderabad, India, pp. 21-24.

Wagstaff, K.M., Glover, D., Tremethick, D.J., Jans, D.A., 2007, Histone-mediated transduction as an efficient means for gene delivery, Molecular Therapy, vol 15, issue 4, Nature Publishing Group, New York NY USA, pp. 721-731.

Wagstaff, K.M., Jans, D.A., 2007, Nucleocytoplasmic transport of DNA: enhancing non-viral gene transfer, Biochemical Journal, vol 406, issue 2, Portland Press Ltd, London England UK, pp. 185-202.

Pouton, C.W., Wagstaff, K.M., Roth, D., Moseley, G.W., Jans, D.A., 2007, Targeted delivery to the nucleus, Advanced Drug Delivery Reviews, vol 59, issue 8, Elsevier Science BV, Amsterdam, pp. 698-717.

Wagstaff, K.M., Jans, D.A., 2006, Intramolecular masking of nuclear localization signals: analysis of importin binding using a novel AlphaScreen-based method, Analytical Biochemistry, vol 348, issue 1, Elsevier Science, San Diego USA, pp. 49-56.

Wagstaff, K.M., Jans, D.A., 2006, Protein transduction: cell penetrating peptides and their therapeutic applications, Current Medicinal Chemistry, vol 13, issue 12, Bentham Science Publishers Ltd, Sharjah UAE, pp. 1371-1387.

Hubner, S., Eam, J.E., Wagstaff, K.M., Jans, D.A., 2006, Quantitative analysis of localization and nuclear aggregate formation induced by GFP-lamin A mutant proteins in living HeLa cells, Journal of Cellular Biochemistry, vol 98, issue 4, Wiley-Liss, Div John Wiley & Sons Inc, Hoboken NJ USA, pp. 810-826.

Ghildyal, R., Ho, A., Wagstaff, K.M., Dias, M.M., Barton, C.L., Jans, P., Bardin, P., Jans, D.A., 2005, Nuclear import of the respiratory syncytial virus matrix protein is mediated by importin beta1 independent of importin alpha, Biochemistry, vol 44, issue 38, American Chemical Society, Washington USA, pp. 12887-12895.

Wagstaff, K.M., Dias, M.M., Alvisi, G., Jans, D.A., 2005, Quantitative analysis of protein-protein interactions by native page/fluorimaging, Journal of Fluorescence, vol 15, issue 4, Springer/Plenum Publishers, New York USA, pp. 469-473.

Postgraduate Research Supervisions

Current Supervision

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Analysis of negative regulators of nuclear import (NRNIs) in spermatogenesis.

Supervisors:

Jans, D (Joint-co), Wagstaff, K (Joint).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Investigation of the Bidirectional Nuclear Transport of Importin 13 and its role in disease.

Supervisors:

Jans, D (Joint-co), Wagstaff, K (Joint).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Non-viral gene delivery and implications for reverse transfection cell microarray technology.

Supervisors:

Jans, D (Joint), Wagstaff, K (Joint-co).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Structural resolution of the interaction between Dengue NS5 and NLS binding partners to determine a basis for chemical inhibition.

Supervisors:

Jans, D (Main), Wagstaff, K (Associate).