Dr Lachlan Gray - Researcher Profile

Biography

Lachlan Gray completed a BSc(Hons) at Melbourne University in 2003. His PhD was completed in 2009 at the Burnet Institute, under the supervision of Prof Paul Gorry. He is a current NHMRC Postdoctoral Fellow at Monash University and the an Honourary Fellow at the Burnet Institute where he studies HIV infection of the CNS. His research interests focus on understanding HIV brain infection and its role in the development of HIV-associated neurocognitive disorders.

Keywords

AIDS--Mental Health, AIDS/HIV, Astrocytes, Brain, CNS, Cure, Eradication, HIV Dementia, Latency

Qualifications

DOCTOR OF PHILOSOPHY

Institution: University of Melbourne

Year awarded: 2009

BACHELOR OF SCIENCE (DEGREE WITH HONOURS)

Institution: University of Melbourne

Year awarded: 2003

BACHELOR OF SCIENCE

Institution: University of Melbourne

Year awarded: 2002

Publications

Journal Articles

Salimi, H., Roche, M., Webb, N., Gray, L.R., Chikere, K., Sterjovski, J., Ellett, A., Wesselingh, S.L., Ramsland, P.A., Lee, B., Churchill, M., Gorry, P.R., 2013, Macrophage-tropic HIV-1 variants from brain demonstrate alterations in the way gp120 engages both CD4 and CCR5, Journal of Leukocyte Biology [P], vol 93, issue 1, Federation of American Societies for Experimental Biology, USA, pp. 113-126.

Hooker, D.J., Mobarok, M., Anderson, J.L., Rajasuriar, R., Gray, L.R., Ellett, A.M., Lewin, S.R., Gorry, P.R., Cherry, C.L., 2012, A new way of measuring apoptosis by absolute quantitation of inter-nucleosomally fragmented genomic DNA, Nucleic Acids Research [P], vol 40, issue 15 (e113), Oxford University Press, UK, pp. 1-13.

Brew, B.J., Gray, L.R., Lewin, S.R., Churchill, M., 2012, Is specific HIV eradication from the brain possible or needed?, Expert Opinion on Biological Therapy [P], vol 13, issue 3, Informa Healthcare, United Kingdom, pp. 403-409.

Gray, L.R., Cowley, D., Crespan, E., Welsh, C., Mackenzie, C., Wesselingh, S.L., Gorry, P.R., Churchill, M.J., 2012, Reduced basal transcriptional activity of Central Nervous System-derived HIV type 1 long terminal repeats, AIDS Research and Human Retroviruses [P], vol 28, issue 00, Mary Ann Liebert Inc. Publishers, USA, pp. 1-6.

Cashin, K., Roche, M., Sterjovski, Ellett, A., Gray, L., Cunningham, A., Ramsland, P., Churchill, M., Gorry, P., 2011, Alternative coreceptor requirements for efficient CCR5- and CXCR4-mediated HIV-1 entry into macrophages, Journal Of Virology [P], vol 85, issue 20, American Society for Microbiology, USA, pp. 10699-10709.

Gray, L., Sterjovski, Ramsland, P., Churchill, M., Gorry, P., 2011, Conformational alterations in the CD4 binding cavity of HIV-1 gp120 influencing gp120-CD4 interactions and fusogenicity of HIV-1 envelopes derived from brain and other tissues, Retrovirology [P], vol 8, issue 42, BioMed Central Ltd, UK, pp. 1-9.

Harman, A., Lai, J., Turville, S., Samarajiwa, S., Gray, L., Marsden, V., Mercier, S., Jones, K., Nasr, N., Rustagi, A., Cumming, H., Donaghy, H., Mak, J., Gale Jr, M., Churchill, M., Hertzog, P., Cunningham, A., 2011, HIV infection of dendritic cells subverts the IFN induction pathway via IRF-1 and inhibits type 1 IFN production, Blood [P], vol 118, issue 2, American Society of Hematology, USA, pp. 298-308.

Sterjovski, J., Roche, M.J., Churchill, M., Ellett, A., Farrugia, W., Gray, L.R., Cowley, D., Poumbourios, P., Lee, B., Wesselingh, S.L., Cunningham, A.L., Ramsland, P.A., Gorry, P.R., 2010, An altered and more efficient mechanism of CCR5 engagement contributes to macrophage tropism of CCR5-using HIV-1 envelopes, Virology [P], vol 404, issue 2, Academic Press, USA, pp. 269-278.

Wightman, F., Solomon, A., Khoury, G., Green, J., Gray, L., Gorry, P.R., Ho, Y., Saksena, N., Hoy, J.F., Crowe, S., Cameron, P., Lewin, S., 2010, Both CD31+and CD31- naive CD4+ T cells are persistent HIV type 1-infected reservoirs in individuals receiving antiretroviral therapy, Journal of Infectious Diseases [E], vol 202, issue 11, Oxford University Press, United States, pp. 1738-1748.

Gray, L.R., Gabuzda, D., Cowley, D., Ellett, A., Chiavaroli, L., Wesselingh, S.L., Churchill, M.J., Gorry, P.R., 2010, CD4 and MHC class 1 down-modulation activities of nef alleles from brain-and lymphoid tissue-derived primary HIV-1 isolates, Journal Of Neurovirology [P], vol 17, issue 1, Springer New York LLC, USA, pp. 82-91.

Loftin, L.M., Kienzle, M.F., Yi, Y., Lee, B., Lee, F., Gray, L.R., Gorry, P.R., Collman, R.G., 2010, Constrained use of CCR5 on CD4+ lymphocytes by R5X4 HIV-1: Efficiency of Env-CCR5 interactions and low CCR5 expression determine a range of restricted CCR5-mediated entry, Virology [P], vol 402, issue 1, Academic Press, USA, pp. 135-148.

Wade, J., Sterjovski, J., Gray, L., Roche, M., Chiavaroli, L., Ellett, A., Jakobsen, M.R., Cowley, D., da Fonseca Pereira, C., Saksena, N., Wang, B., Purcell, D.F.J., Karlsson, I., Fenyo, E.-., Churchill, M., Gorry, P.R., 2010, Enhanced CD4+ cellular apoptosis by CCR5-restricted HIV-1 envelope glycoprotein variants from patients with progressive HIV-1 infection, Virology [P], vol 396, issue 2, Academic Press, USA, pp. 246-255.

Cowley, D., Gray, L.R., Wesselingh, S.L., Gorry, P.R., Churchill, M., 2010, Genetic and functional heterogeneity of CNS-derived tat alleles from patients with HIV-associated dementia, Journal Of Neurovirology [P], vol 17, Springer New York LLC, USA, pp. 70-81.

Gray, L., Roche, M.J., Churchill, M., Sterjovski, J., Ellett, A., Poumbourios, P., Sheffief, S., Wang, B., Saksena, N., Purcell, D., Wesselingh, S.L., Cunningham, A.L., Brew, B.J., Gabuzda, D., Gorry, P.R., 2009, Tissue-specific sequence alterations in the human immunodeficiency virus type 1 envelope favoring CCR5 usage contribute to persistence of dual-tropic virus in the brain, Journal Of Virology [P], vol 83, issue 11, American Society for Microbiology, United States, pp. 5430-5441.

Repits, J., Sterjovski, J., Badia-Martinez, D., Mild, M., Gray, L., Churchill, M.J., Purcell, D.F., Karlsson, A., Albert, J., Fenyo, E.M., Achour, A., Gorry, P.R., Jansson, M., 2008, Primary HIV-1 R5 isolates from end-stage disease display enhanced viral fitness in parallel with increased gp120 net charge, Virology [P], vol 379, issue 1, Academic Press, USA, pp. 125-134.

Sterjovski, J., Churchill, M., Ellett, A., Gray, L.R., Roche, M.J., Dunfee, R., Purcell, D.F., Saksena, N., Wang, B., Sonza, S., Wesselingh, S.L., Karlsson, I., Fenyo, E.M., Gabuzda, D., Cunningham, A.L., Gorry, P.R., 2007, Asn 362 in gp120 contributes to enhanced fusogenicity by CCR5-restricted HIV-1 envelope glycoprotein variants from patients with AIDS, Retrovirology [P], vol 4, BioMed Central Ltd, UK, USA & The Netherlands, pp. 1-21.

Gray, L.R., Churchill, M., Sterjovski, J., Witlox, K.J., Learmont J., Sullivan, J.S., Wesselingh, S.L., Gabuzda, D., Cunningham, A.L., McPhee, D.A., Gorry, P.R., 2007, Phenotype and envelope gene diversity of nef-deleted HIV-1 isolated from long-term survivors infected from a single source, Virology Journal [P], vol 4, BioMed Central Ltd, UK, USA & The Netherlands, pp. 1-12.

Price, P., Keane, N., Gray, L., Lee, S., Gorry, P.R., French, M., 2006, CXCR4 or CCR5 tropism of human immunodeficiency virus type 1 isolates does not determine the immunological milieu in patients responding to antiretroviral therapy, Viral Immunology, vol 19, issue 4, Mary Ann Liebert, Inc. Publishers, USA, pp. 734-740.

Gray, L., Churchill, M., Keane, N., Sterjovski J., Ellett, A., Purcell, D., Poumbourios, P., Kol, C., Wang, B., Saksena, N.K., Wesselingh, S.L., Price, P., French, M., Gabuzda, D., Gorry, P.R., 2006, Genetic and functional analysis of R5X4 human immunodeficiency virus type 1 envelope glycoproteins derived from two individuals homozygous for the CCRdelta532 allele, Journal of Virology, vol 80, issue 7, American Society for Microbiology, USA, pp. 3684-3691.

Churchill, M., Figueiredo, A.P., Cowley, D.J., Gray, L., Purcell, D., Sullivan, J.S., McPhee, D.A., Wesselingh, S.L., Brew, B.J., Gorry, P.R., 2006, Transcriptional activity of blood-and cerebrospinal fluid-derived nef/long-terminal repeat sequences isolated from a slow progressor infected with nef-deleted human immunodeficiency virus type 1 (HIV-1) who developed HIV-associated dementia, Journal of Neurovirology, vol 12, issue 3, Taylor & Francis Inc, PHILADELPHIA USA, pp. 219-228.

Gray, L., Sterjovski J., Churchill, M., Ellery, P., Nasr, N., Lewin, S.R., Crowe, S.M., Wesselingh, S.L., Cunningham, A.L., Gorry, P.R., 2005, Uncoupling coreceptor usage of human immunodeficiency virus type 1 (HIV-1) from macrophage tropism reveals biological properties of CCR5-restricted HIV-1 isolates from patients with acquired immunodeficiency syndrome, Virology, vol 337, issue 2, Academic Press, USA, pp. 384-398.

Churchill, M., Sterjovski J., Gray L., Cowley D., Chatfield C., Learmont J., Sullivan, J.S., Crowe, S.M., Mills, J., Brew, B., Wesselingh, S.L., McPhee, D., Gorry, P.R., 2004, Longitudinal Analysis of nef/Long Terminal Repeat-Deleted HIV-1 in Blood and Cerebrospinal Fluid of a Long-Term Survivor Who Developed HIV-Associated Dementia, The Journal of Infectious Disease, vol 190, Infectious Diseases Society of America, USA, pp. 2181-2186.

Gorry, P., Sterjovski, Churchill, M., Witlox, K., Gray, L., Cunningham, A., Wesselingh, S., 2004, The role of viral coreceptors and enhanced macrophage tropism in human immunodeficiency virus type 1 disease progression, Sexual Health [P], vol 1, issue 1, CSIRO Publishing, Melbourne, Australia, pp. 23-34.

Activities

Affiliations with institutes

From: 01/01/0003 To: 31/12/0013
Burnet Institute
Honorary Research Fellow

Grants

Title:

Mechanicsm involved in the development of HIV-associated dementia.

Investigators:

Gray, L

Funding:

(2010 - 2013). National Health & Medical Research Council (NHMRC). $265,000.00

Postgraduate Research Supervisions

Current Supervision

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

The functional characterisation of HIV-1 Envs derived from the CNS and their role in HIVassociated dementia.

Supervisors:

Churchill, M (Main), Gray, L (Associate).