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Prof Milton Hearn - Researcher Profile

Address

Centre for Green Chemistry
PO Box 23, Clayton

Profile
Summary
Qualifications
Publications
Awards
Supervisions

Biography

Green infusion creates new future for chemistry

Professor Milton Hearn and his team at the Centre for Green Chemistry have developed a new process to extract natural resveratrol from grape waste, rather than manufacturing it from a petro-chemical base. Resveratrol is a natural phenol found in the skin of red grapes and in an increasing number of processed foods, pharmaceuticals and cosmetics. It is sought for its potential anti-ageing and health promoting effects.

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Milton says the new extraction process adds value to what is otherwise agricultural waste. Chemically, it uses a biological process to replace a less efficient synthetic process that produces significant waste of its own. The result of the research is more resveratrol to go around and better environmental outcomes.

The new process uses recyclable polymers to attract and capture the resveratrol from grape skin residues, and waste from the process itself is minimal. It has the potential to generate commercial quantities of resveratrol, making it more readily available for research, and for incorporation into new products. The process can also be adapted to extract other biological phenols, and this is the aim of ongoing research at the Centre.

Milton says all these elements are an important part of the philosophy underpinning the green chemistry approach, which aims to be “benign by design”.

When he first became director of the Centre for Green Chemistry in 2002 the term “green chemistry” related largely to synthetic organic chemistry. Since then he has been instrumental in expanding the concept at both a national and international level, to incorporate what he calls “bio-inspired chemistry”.

As a biochemist, Milton believes taking advantage of existing biological processes often proves a smarter and more cost-effective approach than synthetically reproducing naturally produced chemicals.

He says green chemistry will have an essential role in reducing the environmental and social impacts of the chemical industry. Of the 80,000 or so industrial chemicals used commercially, more than 90 per cent are produced from fossil fuels. In a carbon-aware economy this will have to change. The extraction of biologically produced resveratrol as an alternative to the synthesised product is a small beginning.

Numerous chemical manufacturing processes also generate significant quantities of waste, some of it toxic. In some cases up to 1000 times more waste is generated than usable product. Milton says this is also no longer socially acceptable and that many leading chemical manufacturers are undertaking whole life cycle assessments of their production processes and chemical use. Breaking down their production processes allows these businesses to identify areas where green chemistry can make a difference.

Projects are conducted using flexible, multi-disciplinary teams in much the same way that industry research teams operate. Milton says being able to understand chemistry within a context wider than just the chemistry itself is an essential part of the green approach.

The Centre’s Australian Research Council funding is soon to end, but Milton is confident its next incarnation will provide even stronger links with industry. Monash and the Federal Government are funding a new $78 million purpose-built research centre at Clayton as part of the Green Chemical Futures initiative Milton leads. The new facility will create new opportunities for research, training and collaboration and will improve the capacity of the Australian chemical industry to respond to the challenges of the future.

Milton insists that the Centre’s research is undertaken with practical outcomes in mind.

“Not all our projects are driven by meeting industry needs. But in my view progress in science is only achieved when you can identify the real benefits.”

Qualifications

DOCTOR OF PHILOSOPHY: Institution: University of Adelaide; Year awarded: 2005

Publications

Book Chapters

Boysen, R.I., Hearn, M.T.W., 2012, High perfomance liquid chromatography of peptides and proteins, in Amino acids, peptides and proteins in organic chemistry, eds Andrew B.Hughes, Wiley-VCH, Weinheim Germany, pp. 167-210.

Boysen, R.I., Hearn, M.T.W., 2010, High performance liquid chromatographic separation methods, in Comprehensive Natural Products II: Chemistry and Biology, eds Hung-wen Liu and Lew Mander, Elsevier, Oxford England, pp. 5-49.

Keah, H.H., Hearn, M.T.W., 2003, Purification of large 'troublesome" polypeptides, in In: Proteins and Proteomics, eds Richard J. Simpson, Cold Spring Harbour Laboratory Press, USA, pp. 249-257.

Hennsey, T.P., Huber, M.I., Unger, K.K., Boysen, R.I., Hearn, M.T.W., 2003, Separation of peptides and proteins by high performance capillary electrochromatography (HPCEC), in Proteins and Proteomics: A laboratory manual, Techniques section, Technique 4, eds Richard J. Simpson, Cold Spring Harbor Laboratory Press, USA, pp. 862-868.

Hearn, M.T.W., 2002, Conformational Behaviour of polypeptides and proteins in reversed phase and lipophilic environments, in Biochromatography Theory and Practice, eds M A Vijayalakshmi, Taylor & Francis, London UK, pp. 72-141.

Hearn, M.T.W., 2002, Purification of Synthetic Peptides, in Methods of Organic Chemistry, eds Buchel, Falbe, Hagemann, hanack, Klamann, Kreher, Kropf, Regitz, Schaumann, Thieme, USA, pp. 545-629.

Hearn, M.T.W., 2002, Reversed phase and hydrophobic interaction chromatography of peptides and proteins, in HPLC of Biological Macromolecules, eds Karen M Gooding, Fred E Regnier, Marcel Dekker, Inc, New York NY USA, pp. 99-245.

Hearn, M.T.W., 2001, Conformational behaviour of polypeptides and proteins in reversed phase environments, in Theory and Practice of Biochromatography, eds M.A.Vijayalakshmi, Taylor & Francis Ltd, UK, pp. 72-141.

Boysen, R.I., Hearn, M.T.W., 2001, HPLC of Peptides and Proteins, in Current Protocols in Protein Science, eds J E Coligan, B M Dunn, D W Speicher, P T Wingfield, John Wiley & Sons, USA, pp. 1-40.

Boysen, R.I., Hearn, M.T.W., 2001, HPLC of Peptides and Proteins: Standard Operating Conditions, in Current Protocols in Molecular Biology, eds F M Ausubel, R Brent, R E Kingston, D D Moore, J G Seidman, J A Smith, K Struhl, John Wiley & Sons, USA, pp. 1-32.

Boysen, R.I., Hearn, M.T.W., 2001, HPLC of peptides and proteins: standard operating conditions, in Current Protocols in Molecular Biology, eds Lisa Albright, Donald Coen, Ajit Varki, John Wiley & Sons, Inc, USA, pp. 10.13.1-10.13.32.

Boysen, R.I., Hearn, M.T.W., 2001, Purification of Proteins by High-Performance Liquid Chromatography, in Current Protocols in Molecular Biology, eds F M Ausubel, R Brent, R E Kingston, D D Moore, J G Seidman, J A Smith, K Struhl, John Wiley and Sons, USA, pp. 1-9.

Boysen, R.I., Hearn, M.T.W., 2001, Purification of proteins by high-performace liquid chromatography. HPLC of peptides and proteins: preparation and system set-up, in Current Protocols in Molecular Biology, eds Lisa Albright, Donald Coen, Ajit Varki, John Wiley & Sons, USA, pp. 10.12.1-10.12.9.

Hearn, M.T.W., 2000, Physicochemical factors in polypeptide and protein purification and analysis by high-performance liquid chromatographic techniques: Surrent status and challenges for the Future, in Handbook of Bioseparations, Academic Press, San Diego USA, pp. 71-235.

Hearn, M.T., 1998, High-Resolution Reversed-Phase Chromatography, in Protein Purification - Principles, High Resolution Methods, and Applications, John Wiley & Sons, Inc., New York NY USA, pp. 239-282.

Journal Articles

Komaromy, A.z., Li, S., Zhang, H., Nicolau, D.V., Boysen, R.I., Hearn, M.T.W., 2012, Arrays of nano-structured surfaces to probe the adhesion and viability of bacteria, Microelectronic Engineering [P], vol 91, Elsevier Science BV, Amsterdam Netherlands, pp. 39-43.

Bhagwat, A.M., Campi, E.M., Potdar, M.K., Jackson, W.R., Hearn, M.T.W., 2012, Efficient nucleophilic substitution of halopyridines using ethanol as solvent with microwave heating: synthesis of 2-aminoethylsulfanylpyridines, Green Chemistry Letters and Reviews [P], vol 5, issue 4, Taylor & Francis, United Kingdom, pp. 595-601.

Do Pham, D.D., Kelso, G., Yang, Y., Hearn, M.T.W., 2012, One-pot oxidative N-demethylation of tropane alkaloids with hydrogen peroxide and a Fe-III-TAML catalyst, Green Chemistry [P], vol 14, issue 4, Royal Society of Chemistry, Cambs England, pp. 1189-1195.

Boysen, R.I., Li, S., Chowdhury, J., Schwarz, L., Hearn, M.T.W., 2012, Selectivity optimisation of biomimetic molecularly imprinted polymer thin films, Microelectronic Engineering [P], vol 97, Elsevier Science BV, Amsterdam Netherlands, pp. 81-84.

Ciayadi, R., Potdar, M., Walton, K., Harrison, G., Kelso, G., Harris, S., Hearn, M., 2011, 2-Phenyl and 2-heterocyclic-4-(3-(pyridin-2-yl)-1H-pyrazol-4-yl)pyridines as inhibitors of TGF-B1 and activin A signalling, Bioorganic & Medicinal Chemistry Letters [P], vol 21, issue 18, Pergamon-Elsevier Science Ltd, Oxford England, pp. 5642-5645.

Kaur, G., Chang, L., Bell, T., Hearn, M., Saito, K., 2011, Bioinspired core-crosslinked micelles from thymine-functionalized amphiphilic block copolymers: hydrogen bonding and photo-crosslinking study, Journal Of Polymer Science Part A-Polymer Chemistry [P], vol 49, issue 19, Wiley-Blackwell, Malden Massachusetts USA, pp. 4121-4128.

Graham, B., Spiccia, L., Hearn, M., 2011, Comparison of the binding behavior of several histidine-containing proteins with immobilized copper(II) complexes of 1,4,7-triazacyclononane and 1,4-bis(1,4,7-triazacyclononan-1-yl)butane, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences [P], vol 879, issue 13-14, Elsevier Science BV, Netherlands, pp. 844-852.

Widakowich, G., Zhang, C., Harris, S., Mitri, K., Powers, G., Troung, K., Hearn, M., 2011, Effects of IMAC specific peptide tags on the stability of recombinant green fluorescent protein, Biotechnology Progress [P], vol 27, issue 4, Wiley-Blackwell, USA, pp. 1048-1053.

Schwarz, L., Danylec, B., Yang, Y., Harris, S., Boysen, R., Hearn, M., 2011, Enrichment of (E)-resveratrol from peanut byproduct with molecularly imprinted polymers, Journal of Agricultural and Food Chemistry [P], vol 59, issue 8, American Chemical Society, Washington Detroit USA, pp. 3539-3543.

Saito, K., Pant, S., Hearn, M., 2011, Oxidative polymerization of 2,6-dimethylphenol in water using bis-triazacyclononane copper catalyst, Journal Of Applied Polymer Science [P], vol 122, issue 4, Wiley-Blackwell, Malden Massachusetts USA, pp. 2174-2180.

Schwarz, L., Danylec, B., Harris, S., Boysen, R., Hearn, M., 2011, Preparation of molecularly imprinted polymers for the selective recognition of the bioactive polyphenol, (E)-resveratrol, Journal of Chromatography A [P], vol 1218, issue 16, Elsevier Science BV, Amsterdam Netherlands, pp. 2189-2195.

Boysen, R., Yang, Y., Chowdhury, J., Matyska, M., Pesek, J., Hearn, M., 2011, Simultaneous separation of hydrophobic and hydrophilic peptides with a silica hydride stationary phase using aqueous normal phase conditions, Journal of Chromatography A [P], vol 1218, issue 44, Elsevier Science BV, Amsterdam Netherlands, pp. 8021-8026.

Young, J., Thienthong, N., Hearn, M., Robinson, A., Jackson, W., 2011, Synthesis and evaluation of potentially transdermal morphine derivatives, Australian Journal of Chemistry [E], vol 64, issue 10, CSIRO Publishing, Collingwood Vic Australia, pp. 1339-1345.

Mountford, S., Campi, E., Robinson, A., Hearn, M., 2011, Synthesis of N-heterocyclic ligands for use in affinity and mixed mode chromatography, Tetrahedron [P], vol 67, issue 2, Pergamon Elsevier Science Ltd, Oxford England, pp. 471-485.

Young, J., Thienthong, N., Hearn, M., Jackson, W., 2011, Unexpected formation of tetramethylguanidines by reaction of dimethylformamide with Fmoc-protected aminoamides, Synlett [P], vol 6, Georg Thieme Verlag KG, Germany, pp. 831-833.

Mooney, J., Fredericks, D., Hearn, M., 2011, Use of phage display methods to identify heptapeptide sequences for use as affinity purification 'tags' with novel chelating ligands in immobilized metal ion affinity chromatography, Journal of Chromatography A [P], vol 1218, issue 1, Elsevier BV, Netherlands, pp. 92-99.

Pant, S., Hearn, M.T.W., Saito, K., 2010, 1,4,7-tricarboxymethyl-1,4,7-triazacyclononane copper catalyzed oxidative polymerization to form poly(2,6-dimethyl-1,4-phenylene oxide) in water, Australian Journal of Chemistry [E], vol 63, issue 3, CSIRO Publishing, Collingwood Vic Australia, pp. 502-506.

Li, S., Komaromy, A.z., Nicolau, D.V., Boysen, R.I., Hearn, M.T.W., 2010, Bacterial adhesion to toroidal nano-structures from poly(styrene)-block-poly(tert-butyl acrylate) diblock copolymer thin films, Microelectronic Engineering [P], vol 87, issue 5-8, Elsevier Science BV, Amsterdam Netherlands, pp. 715-718.

Gibbs, G., Lo, C.Y.J., Nixon, B., Jamsai, D., O'Connor, A., Rijal, S., Sanchez-Partida, L.G., Hearn, M.T.W., Bianco, D.M., O'Bryan, M.K., 2010, Glioma pathogenesis-related 1-like 1 is testis enriched, dynamically modified, and redistributed during male germ cell maturation and has a potential role in sperm-oocyte binding, Endocrinology [P], vol 151, issue 5, The Endocrine Society, USA, pp. 2331-2342.

Fredericks, D., Clay, R., Warner, T., O'Connor, A., de Kretser, D., Hearn, M., 2010, Optimization of the expression of recombinant human activin A in the yeast Pichia pastoris, Biotechnology Progress [P], vol 26, issue 2, John Wiley & Sons LTD, Sussex England, pp. 372-383.

Okayasu, T., Saito, K., Nishide, H., Hearn, M., 2010, Poly(vinylsulfonic acid)-grafted solid catalysts: new materials for acid-catalysed organic synthetic reactions, Green Chemistry [P], vol 12, issue 11, Royal Society of Chemistry, Cambridge England, pp. 1981-1989.

Alam, A., Mataj, A., Yang, Y., Boysen, R., Bowden, D., Hearn, M., 2010, Rapid microwave-assisted chemical cleavage - mass spectrometric method for the identification of hemoglobin variants in blood, Analytical Chemistry [P], vol 82, issue 21, American Chemical Society, Washington DC USA, pp. 8922-8930.

Mountford, S.J., Campi, E.M., Robinson, A.J., Hearn, M.T.W., 2010, S -> N type Smiles rearrangement of the aminoethanethiol moiety attached to heterocyclic compounds, Tetrahedron [P], vol 66, issue 19, Pergamon Elsevier Science LTD, Oxford England, pp. 3452-3456.

Johnston, P., Hearn, M.T.W., Saito, K., 2010, Solid-state photoreversible polymerization of n-alkyl-linked bis-thymines using non-covalent polymer-templating, Australian Journal of Chemistry [E], vol 63, issue 4, CSIRO Publishing, Collingwood Vic Australia, pp. 631-639.

Kazi, S., Kelso, G., Harris, S., Boysen, R., Chowdhury, J., Hearn, M., 2010, Synthesis of quinoline thioethers as novel small molecule enhancers of monoclonal antibody production in mammalian cell culture, Tetrahedron [P], vol 66, issue 48, Pergamon Elsevier Science Ltd, Oxford England, pp. 9461-9467.

Pesek, J.J., Matyska, M., Hearn, M.T.W., Boysen, R.I., 2009, Aqueous normal-phase retention of nucleotides on silica hydride columns, Journal of Chromatography A [P], vol 1216, issue 7, Elsevier BV, Netherlands, pp. 1140-1146.

Tanaka, M., Jokubaitis, V., Wood, C., Wang, Y., Brouard, N., Pera, M.F., Hearn, M.T.W., Simmons, P.J., Nakayama, N., 2009, BMP inhibition stimulates WNT-dependent generation of chondrogenic mesoderm from embryonic stem cells, Stem Cell Research [P], vol 3, issue 2-3, Elsevier BV, The Netherlands, pp. 126-141.

Fadhel, B.A., Hearn, M.T.W., Chaffee, A.L., 2009, CO2 adsorption by PAMAM dendrimers: Significant effect of impregnation into SBA-15, Microporous and Mesoporous Materials [P], vol 123, Elsevier, Netherlands, pp. 140-149.

Maharjan, P., Hearn, M.T.W., Jackson, W.R., DeSilva, K., Woonton, B., 2009, Development of a temperature-responsive agarose-based ion-exchange chromatographic resin, Journal of Chromatography A [P], vol 1216, Elsevier, Netherlands, pp. 8722-8729.

Held, M., Komaromy, A.z., Fulga, F., Edwards, C., Boysen, R.I., Hearn, M.T.W., Nicolau, D.V., 2009, Dynamic behaviour of microorganisms on microstructures, Microelectronic Engineering [P], vol 86, Elsevier BV, Netherlands, pp. 1455-1458.

Papakonstantinou, T., Harris, S.J., Hearn, M.T.W., 2009, Expression of GFP using pichia pastoris vectors with zeocin or g-418 sulphate as the primary selectable marker, Yeast [P], vol 26, Wiley Interscience, United Kingdom, pp. 311-321.

Yang, Y., Boysen, R.I., Hearn, M.T.W., 2009, Hydrophilic interaction chromatography coupled to electrospray mass spectrometry for the separation of peptides and protein digests, Journal of Chromatography A [P], vol 1216, issue 29, Elsevier BV, Netherlands, pp. 5518-5524.

Komaromy, A.z., Boysen, R.I., Zhang, H., McKinnon, I.R., Fulga, F., Hearn, M.T.W., Nicolau, D., 2009, Micro-structures modulate bacterial cell viability and attachment, Microelectronic Engineering [P], vol 86, issue 4, Elsevier BV, Netherlands, pp. 1431-1434.

Yang, Y., Boysen, R.I., Harris, S.J., Hearn, M.T.W., 2009, Peptide mapping with mobile phases of intermediate pH value using capillary reversed-phase high-performance liquid chromatography/electrospray ionisation tandem mass spectrometry, Journal of Chromatography A [P], vol 1216, issue 18, Elsevier BV, Netherlands, pp. 3767-3773.

Okayasu, T., Saito, K., Nishide, H., Hearn, M.T.W., 2009, Preparation of a novel poly(vinylsulfonic acid)-grafted solid phase acid catalyst and its use in esterification reactions, Chemical Communications [P], Royal Chemical Society, UK, pp. 4708-4710.

Jiang, W., Prescott, M., Devenish, R.J., Spiccia, L., Hearn, M.T.W., 2009, Separation of hexahistidine fusion proteins with immobilized metal ion affinity chromatographic (IMAC) sorbents derived from MN+ -tacn and its derivatives, Biotechnology And Bioengineering [P], vol 103, issue 4, Wiley Periodicals Inc, Germany, pp. 747-756.

Papakonstantinou, T., Harris, S.J., Fredericks, D.P., Harrison, C.A., Wallace, E.M., Hearn, M.T.W., 2009, Synthesis, purification and bioactivity of recombinant human activin A expressed in the yeast pichia pastoris, Protein Expression and Purification, vol 64, Elsevier, Netherlands, pp. 131-138.

Kreher, U., Hearn, M.T.W., Spiccia, L., 2009, X-ray crystal structure, acidbase properties and complexation characteristics of a methylenephosphonate derivative of 1,4,7,10- tetraazacyclododecane, Australian Journal of Chemistry [E], vol 62, CSIRO, Melbourne, Australia, pp. 1583-1592.

Fadhel, B.A., Hearn, M.T.W., Chaffee, A.L., 2008, CO2 adsorption by neat versus impregnated pamam dendrimers, Fuel Chemistry Division, Preprints, vol 53, issue 2, American Chemical Society, USA, pp. 731-732.

Wang, Y., Boysen, R.I., Wood, B.R., Kansiz, M., McNaughton, D., Hearn, M.T.W., 2008, Determination of the secondary structure of proteins in different environments by FTIR-ATR spectroscopy and PLS regression, Biopolymers, vol 89, issue 11, Wiley Interscience, USA, pp. 895-905.

Graham, B., Spiccia, L., Hearn, M.T.W., 2008, Examination of the binding behaviour of several proteins with the immobilized copper(II) complexes of o-, m- and p-xylylene bridged bis(1,4,7-triazacyclononane) macrocycles, Journal of Chromatography A, vol 1194, Elsevier, The Netherlands, pp. 30-37.

Perchyonok, V.T., Ryan, S.J., Langford, S.J., Hearn, M.T.W., Tuck, K.L., 2008, Facile and selective deallylations of esters under 'aqueous' free-radical conditions, Synlett, Georg Thieme Verlag, Germany, pp. 1233-1235.

Foulds, L.M., Boysen, R.I., Crane, M., Yang, Y., Muir, J.A., Smith, A.I., de Kretser, D.M., Hearn, M.T.W., Hedger, M.P., 2008, Molecular Identification of lyso-glycerophosphocholines as endogenous immunosuppressives in bovine and rat gonadal fluids, Biology of Reproduction, vol 79, issue 3, Society for the Study of Reproduction, United States, pp. 525-536.

Maharjan, P., Woonton, B., Bennett, L.E., Smithers, G.W., DeSilva, K., Hearn, M.T.W., 2008, Novel chromatographic separation - The potential of smart polymers, Innovative Food Science & Emerging Technologies, vol 9, Elsevier, The Netherlands, pp. 232-242.

Perchyonok, V.T., Tuck, K.L., Langford, S.J., Hearn, M.T.W., 2008, On the scope of radical reactions in aqueous media utilizing quaternary ammonium salts of phosphinic acids as chiral and achiral hydrogen donors, Tetrahedron Letters, vol 49, issue 32, Elsevier, UK, pp. 4777-4779.

Graham, B., Comba, P., Hearn, M.T.W., Spiccia, L., 2007, An examination of the binding behavior of histidine-containing peptides with immobilized metal complexes derived from the macrocyclic ligand, 1,4,7-triazacyclononane, Journal of Biological Inorganic Chemistry, vol 12, issue 1, Springer, Germany, pp. 11-21.

Kreher, U., Hearn, M.T.W., Moubaraki, B., Murray, K.S., Spiccia, L., 2007, Coordination chemistry of 1,4-bis(carboxymethyl)-1,4,7-triazacyclononane: Synthesis and characterization of mononuclear and binuclear mu-oxo-bridged iron(III) complexes, and a 1D-helical copper(II) chain, Polyhedron, vol 26, Elsevier, Netherlands, pp. 3205-3216.

Yang, Y., Boysen, R.I., Matyska, M., Pesek, J.J., Hearn, M.T.W., 2007, Open-Tubular capillary electrochromatography coupled with electrospray ionization mass spectrometry for peptide analysis, Analytical Chemistry, vol 79, issue 13, American Chemical Society, USA, pp. 4942-4949.

Pesek, J.J., Matyska, M., Hearn, M.T.W., Boysen, R.I., 2007, Temperature effects on solute retention for hydride-based stationary phases, Journal of Separation Science, vol 30, Wiley - V C H Verlag GmbH & Co. KGaA, Germany, pp. 1150-1157.

Davidson, K.C., Jamshidi, P., Daly, R., Hearn, M.T.W., Pera, M.F., Dottori, M., 2007, Wnt3a regulates survival, expansion, and maintenance of neural progenitors derived from human embryonic stem cells, Molecular and Cellular Neuroscience, vol 36, issue 3, Academic Press Inc Elsevier Science, San Diego United States, pp. 408-415.

Boysen, R., Hearn, M., 2006, Desalting of peptides and protein mixtures by RP-HPLC techniques, Cold Spring Harbor Protocols [E], vol 5, Cold Spring Harbor Laboratory, USA, pp. 1-6.

Daly, R., Hearn, M.T.W., 2006, Expression of the human activin type I and II receptor extracellular domains in Pichia pastoris, Protein Expression and Purification, vol 46, issue 2, Academic Press, USA, pp. 456-467.

Yang, Y., Boysen, R.I., Hearn, M.T.W., 2006, Optimization of field-amplified sample injection for analysis of peptides by capillary electrophoresis-mass spectrometry, Analytical Chemistry, vol 78, issue 14, American Chemical Society, USA, pp. 4752-4758.

Boysen, R., Hearn, M., 2006, Purification of peptides from solid-phase peptide synthesis with RP-HPLC, Cold Spring Harbor Protocols [E], vol 5, Cold Spring Harbor Laboratory, USA, pp. 1-9.

Keah, H.H., Hearn, M.T.W., 2005, A molecular recognition paradigm: Promiscuity associated with the ligand-receptor interactions of the activin members of the TGF-beta superfamily, Journal of Molecular Recognition, vol 18, John Wiley & Sons Ltd, Chichester Uk, pp. 385-403.

Warden, A.C., Warren, M., Hearn, M.T.W., Spiccia, L., 2005, Adduct formation between organic oxoanions and hexaazamacrocycles, Crystal Growth & Design, vol 5, issue 2, American Chemical Society, Washington USA, pp. 713-720.

Boysen, R.I., Jong, A.J.O., Hearn, M.T.W., 2005, Binding behaviour and conformational properties of globular proteins in the presence of immobilised non-polar ligands used in reversed-phase liquid chromatography, Journal of Chromatography A, vol 1079, Elsevier Science BV, Amsterdam The Netherlands, pp. 173-186.

Daly, R., Hearn, M.T.W., 2005, Expression of heterologous proteins in Pichia pastoris: A useful experimental tool in protein engineenring and production, Journal of Molecular Recognition, vol 18, issue 2, John Wiley & Sons Ltd, Chichester England, pp. 119-138.

Matyska, M., Pesek, J.J., Chen, J.I., Boysen, R.I., Hearn, M.T.W., 2005, Probing with synthetic peptides the interactive properties of N-butylphenyl and cholesterol-10-undecenaoate phases immobilised onto open tubular electrochromatographic capillaries, Chromatographia, vol 61, issue 7/8, Vieweg, Germany, pp. 351-357.

Warden, A.C., Spiccia, L., Hearn, M.T.W., Boas, J.F., Pilbrow, J.R., 2005, The synthesis, structure and properties of copper(II) complexes of asymmetrically functionalized derivatives of 1,4,7-triazacyclononane, Dalton Transactions, Royal Society of Chemistry, Cambridge England, pp. 1804-1813.

Yang, Y., Boysen, R.I., Hearn, M.T.W., 2005, Use of mixed-mode sorbents for the electrochromatographic separation of thrombin receptor antagonistic peptides, Journal of Chromatography A, vol 1079, issue 1-Feb, Elsevier Science BV, Amsterdam The Netherlands, pp. 328-334.

Warden, A.C., Warren, M., Battle, A.R., Hearn, M.T.W., Spiccia, L., 2004, Adducts formed by tetrahedral anions and protonated forms of 1,4,7-triazacyclononane: competition with chloride anions, CrystEngComm, vol 6, issue 84, Royal Society of Chemistry, Cambridge England, pp. 522-530.

Yang, Y., Boysen, R.I., Hearn, M.T.W., 2004, Analysis of synthetic peptides by capillary electrophoresis: effect of organic solvent modifiers and variable electrical potentials on separation efficiencies, Journal of Chromatography A, vol 1043, Elsevier, Amsterdam The Netherlands, pp. 91-97.

Warden, A.C., Warren, M., Hearn, M.T.W., Spiccia, L., 2004, Anion binding to azamacrocycles: synthesis and X-ray crystal structures of halide adducts of [12]aneN4 and [18]aneN6, New Journal of Chemistry, vol 28, Royal Society of Chemistry, Cambridge England, pp. 1160-1167.

Warden, A.C., Warren, M., Hearn, M.T.W., Spiccia, L., 2004, Binding of inorganic oxoanions to macrocyclic ligands: effect of the degree of protonation on supramolecular assemblies formed by phosphate and [18]aneN6, Inorganic Chemistry, vol 43, issue 22, American Chemical Society, Washington USA, pp. 6936-6943.

Warden, A.C., Warren, M., Hearn, M.T.W., Spiccia, L., 2004, Binding of inorganic oxoanions to macrocyclic ligands: interactions of sulfate and dithionate with protonated forms of [18]aneN6, New Journal of Chemistry, vol 28, Royal Society of Chemistry, Cambridge England, pp. 1301-1308.

O'Bryan, M.K., Foulds, L.M., Cannon, J.F., Winnall, W.R., Muir, J.A., Sebire, K.L., Smith, A.I., Keah, H.H., Hearn, M.T.W., de Kretser, D.M., Hedger, M.P., 2004, Identification of a Novel Apolipoprotein, ApoN, in Ovarian Follicular Fluid, Endocrinology, vol 145, issue 11, The Endocrine Society, USA, pp. 5231-5242.

Yang, Y., Boysen, R.I., Hearn, M.T.W., 2004, Impact of organic solvents on the resolution of synthetic peptides by capillary electrophoresis, Journal of Chromatography A, vol 1043, Elsevier, Amsterdam The Netherlands, pp. 81-89.

Pesek, J.J., Matyska, M., Dawson, G.B., Chen, J.I., Boysen, R.I., Hearn, M.T.W., 2004, Open tubular capillary electrochromatography of synthetic peptides on etched chemically modified columns, Analytical Chemistry, vol 76, issue 1, American Chemical Society, Washington USA, pp. 23-30.

Pesek, J.J., Matyska, M., Brent-Dawson, G., Chen, J.I., Boysen, R.I., Hearn, M.T.W., 2004, Open-tubular electrochromatographic characterization of synthetic peptides, Electrophoresis, vol 25, Wiley, Germany, pp. 1211-1218.

Graham, B., Hearn, M.T.W., Spiccia, L., Skelton, B.W., White, A.H., 2003, Amino acid binding to copper(II) in (1,4,7-triazacyclononane) (L-histidine)copper(II) hexafluorophosphate monohydrate, Australian Journal of Chemistry, vol 56, CSIRO Publishing, Collingwood Vic Australia, pp. 1259-1261.

Warden, A.C., Hearn, M.T.W., Spiccia, L., 2003, Novel acetate binding modes in [Na2Cu(CH3COO)4(H2O)]H2O, Inorganic Chemistry, vol 42, American Chemical Society, Washington USA, pp. 7037-7040.

Walhagen, K., Huber, M.I., Hennessy, T., Hearn, M.T.W., 2003, On the nature of the forces controlling selectivity in the high performance capillary electrochromatographic (HP-CEC) separation of peptides, Biopolymers, vol 1, John Wiley & Sons, Inc., USA, pp. 429-453.

Pesek, J.J., Matyska, M., Dawson, G.B., Chen, J.I., Boysen, R.I., Hearn, M.T.W., 2003, Open tubular capillary electrochromatography of synthetic peptides on etched chemically modified columns, Analytical Chemistry, vol 76, issue 1, American Chemical Society, Washington USA, pp. 23-30.

Hennessy, T., Boysen, R.I., Huber, M.I., Unger, K.K., Hearn, M.T.W., 2003, Peptide mapping by reversed-phase high-performance liquid chromatography employing silica rod monoliths, Journal of Chromatography A, vol 1009, Elsevier, Amsterdam The Netherlands, pp. 15-28.

Yang, Y., Boysen, R.I., Chen, J.I., Keah, H.H., Hearn, M.T.W., 2003, Separation of structurally related synthetic peptides by capillary zone electrophoresis, Journal of Chromatography A, vol 1009, Elsevier, Amsterdam The Netherlands, pp. 3-14.

Walhagen, K., Boysen, R.I., Hearn, M.T.W., Unger, K.K., 2003, The CEC Behaviour of several synthetic peptides related to the activin beta A - beta D subunits, International Journal of Peptide Research and Therapeutics, vol 61, Springer, New York NY USA, pp. 109-121.

Unger, K.K., Huber, M., Walhagen, K., Hennessy, T., Hearn, M.T.W., 2002, A Critical Appraisal of Capillary Electrochromatography, Analytical Chemistry, vol 74, issue 7, American Chemical Society, Washington DC USA, pp. 200A-207A.

Walhagen, K., Unger, K.K., Keah, H., Hearn, M.T.W., 2002, Application of CEC procedures for the analysis of synthetic peptides: characterization of linear immunogenic peptides that mimic a HIV-1 gp120 epitope, Journal of Peptide Research, vol 59, issue 4, Blackwell Munksgaard, Copenhagen Denmark, pp. 159-173.

Chowdury, J., Boysen, R.I., Ihara, H., Hearn, M.T.W., 2002, Binding Behaviour of Crystalline and noncrystalline Phases: Evaluation of the Enthalpic and Entropic Contributions to the Separation Selectivity of Nonpolar Solutes with a Novel Chromatographic Sorbent, Journal of Physical Chemistry B [P], vol 106, American Chemical Society, Washington DC USA, pp. 11936-11950.

Hickox, D., Gibbs, G., Morrison, J.R., Sebire, K.L., Edgar, K., Keah, H., Alter, K., Loveland, K.L., Hearn, M.T.W., de Kretser, D.M., O'Bryan, M.K., 2002, Identification of a novel testis-specific member of the phosphatidykethanolamine binding protein family, pebp-2, Biology of Reproduction, vol 67, Society for the study of Reproduction, Madison WI USA, pp. 917-927.

I, T., Smith, R., Guhan, S., Taksen, K., Vavra, M., Myer, D., Hearn, M.T.W., 2002, Intelligent automation of high-performance liquid chromatogrpahy method development by means of a real-time knowledge-based approach, Journal of Chromatography A, vol 972, Elsevier, Amsterdam Netherlands, pp. 27-43.

Boysen, R.I., Jong, A., Wilce, J.A., King, G.F., Hearn, M.T.W., 2002, Role of Interfacial Hydrophobic Residues in the Stabilization of the Leucine Zipper structures of the transcription Factors c-Fos and c-Jun, The Journal of Biological Chemistry, vol 277, issue 1, The American Society for Biochemistry and Molecular Biology I, Bethesda MD USA, pp. 23-31.

Graham, B., Spiccia, L., Fallon, G.D., Hearn, M.T.W., Mabbs, F.E., Moubaraki, B., Murray, K.S., 2002, Structure and magnetic properties of tri- and hexa-nuclear hydroxo-bridged copper(II) complexes formed by a trimacrocyclic derivative of 1,4,7-triazacyclononane, Journal of the Chemical Society - Dalton Transactions, vol 6, Royal Society of Chemistry, Cambridge England, pp. 1226-1232.

Hearn, M.T.W., Quirino, J.P., Whisstock, J.C., Terabe, S., 2002, Thermal Unfolding of Proteins Studied by Coupled Reversed-Phase HPLC-Electrospray Ionizatin Mass Spectronometry Techniques Based on Isotope Exchange Effects, Analytical Chemistry, vol 74, issue 6, American Chemical Society, Washington DC USA, pp. 1467-1475.

Boysen, R.I., Jong, A., Hearn, M.T.W., 2002, Thermodynamic Assessment of the Stability of Thromic Receptor Antagonistic Peptides in Hydrophobic Environments, Biophysical Journal, vol 82, Biophysical Society, Bethesda MD USA, pp. 2279-2292.

Lin, F., Chen, W., Hearn, M.T.W., 2002, Thermodynamic analysis of the interaction between proteins and solid surfaces; application to liquid chromatography, Journal of Molecular Recognition, vol 15, John Wiley & Sons Ltd, Chichester England, pp. 55-93.

Hearn, M.T., Acosta, D.B., 2001, Applications of novel affinity cassette methods: use of peptide fusion handles for the purification of recombinant proteins, Journal of Molecular Recognition, vol 14, issue 6, John Wiley & Sons Ltd, West Sussex England, pp. 323-369.

Walhagen, K., Unger, K., Hearn, M.T., 2001, Capillary electrochromatrography analysis of hormonal cyclic and linear peptides, Analytical Chemistry, vol 73, issue 20, American Chemical Society, Washington DC USA, pp. 4924-4936.

Matyska, M., Pesek, J.J., Boysen, R.I., Hearn, M.T., 2001, Characterization and applications of etched chemically modified capillaries for open-tubular capillary electrochromatography, Electrophoresis, vol 22, Wiley - VCH Verlag GMBH, Berlin Germany, pp. 2620-2628.

Matyska, M., Pesek, J.J., Boysen, R.I., Hearn, M.T., 2001, Characterization of open tubular capillary electrochromatography columns for the analysis of synthetic peptides using isocratic conditions, Analytical Chemistry, vol 73, issue 21, American Chemical Society, Washington DC USA, pp. 5116-5125.

Hearn, M.T.W., Anspach, B., 2001, Chemical, physical and biochemical concepts in isolation and purification of proteins, , vol 30, pp. 221-263.

Matyska, M., Pesek, J.J., Boysen, R.I., Hearn, M.T., 2001, Electrochromatographic characterization of etched chemically-modified capillaries with small synthetic peptides, Journal of Chromatography A, vol 924, issue 1-2, Elsevier Science BV, Amsterdam Netherlands, pp. 211-221.

Lin, F., Chen, W., Hearn, M.T., 2001, Microcalorimetric studies on the interaction mechanism between proteins and hydrophobic solid surfaces in hydrophobic interaction chromatography: Effects of salts, hydrophobicity of the sorbent, and structure of the protein, Analytical Chemistry, vol 73, issue 16, American Chemical Society, Washington DC USA, pp. 3875-3883.

Hearn, M.T., 2001, Peptide analysis by rapid, orthogonal technologies with high separation selectivities and sensitivities, Biologicals, vol 29, Academic Press Ltd, London England, pp. 159-178.

Guo, M.L., Hearn, M.T.W., Sitaram, B.R., 2001, Relationship between the reversed phase high performance liquid chromatographic retention behavior of the prion 113-120 peptide and stability of its conformation, Fenxi Huaxue, vol 29, Zhongguo Kexueyuan, Changchun Yingyong Huaxue Yanjiusuo, China, pp. 633-636.

Cavallaro, V., Thompson, P.E., Hearn, M.T.W., 2001, Solid-phase synthesis of a dedritic peptide related to a retinoblastoma protein fragment utilizing a combined boc- and fmoc- chemistry approach, Journal of Peptide Science, vol 7, issue 5, John Wiley & Sons Ltd, West Sussex England, pp. 262-269.

Cavallaro, V., Thompson, P.E., Hearn, M.T., 2001, Solid-phase synthesis of cyclic analogues related to the hypoglycaemic peptide hGH(6-13): Comparison of two i i +4 lactam cyclization procedures, Journal of Peptide Science, vol 7, issue 10, John Wiley & Sons Ltd, West Sussex England, pp. 529-536.

Graham, B., Spiccia, L., Bond, A.M., Hearn, M.T., Kepert, C.M., 2001, Structural, spectroscopic and electrochemical studies of nickel(II) "sandwich" complexes with ligands featuring tethered 1,4,7-triazacyclononane macrocycles, Dalton Transactions: an international journal of inorganic chemistry, vol 15, issue 15, Royal Society of Chemistry, UK, pp. 2232-2238.

Graham, B., Hearn, M.W., Junk, P.C., Kepert, C.M., Mabbs, F.E., Moubaraki, B., Murray, K.S., Spiccia, L., 2001, Syntheses, crystal structures, magnetic properties, and EPR spectra of tetranuclear copper(II) complexes featuring pairs of "roof-shaped" Cu2X2 dimers with hydroxide, methoxide, and azide bridges, Inorganic Chemistry, vol 40, issue 7, American Chemical Society, USA, pp. 1536-1543.

Keah, H., O'Bryan, M., de Kretser, D.M., Hearn, M.T., 2001, Synthesis and application of peptide immunogens related to the sperm tail protein tpx-1, a member of the CRISP superfamily of proteins, Journal of Peptide Science, vol 57, John Wiley & Sons Ltd, West Sussex England, pp. 1-10.

Warden, A.C., Graham, B., Hearn, M.T.W., Spiccia, L., 2001, Synthesis of novel derivatives of 1,4,7-triazacyclononane, Organic Letters, vol 3, issue 18, American Chemical Society, USA, pp. 2855-2858.

Guo, M.L., Hearn, M.T.W., Keah, H.H., 2001, The RP-HPLC retention behaviour of three peptides from MAP2 and their relations to the structure properties of the peptides, Journal of Biochemistyr, Molecular Biology and Biophysics, vol 5, Taylor & Francis Ltd, UK, pp. 383-391.

Boysen, R.I., Hearn, M.T., 2001, The metal binding properties of the CCCH motif of the 50S ribosomal protein L36 from Thermus thermophilus, Journal of Peptide Research, vol 57, Munksgaard International Publishing Ltd, Copenhagen Denmark, pp. 19-28.

Keah, H., Allen, N.S., Clay, R.L., Boysen, R.I., Warner, T., Hearn, M.T., 2001, Total chemical synthesis of human activin bA [12-116] and related large-loop polypeptides, Biopolymers, vol 60, issue 4, John Wiley & Sons Inc, Chichester England, pp. 279-289.

O'Bryan, M., Sebire, K.L., Meinhardt, A., Edgar, K., Keah, H., Hearn, M.T., de Kretser, D.M., 2001, Tpx-1 is a component of the outer dense fibers and acrosome of rat spermatozoa, Molecular Reproduction and Development, vol 58, Wiley-Liss, New York NY USA, pp. 116-125.

Zachariou, M., Hearn, M.T.W., 2000, Adsorption and selectivity characteristics of several human serum proteins with immobilised hard Lewis metal ion-chelate adsorbents, Journal of Chromatography A, vol 890, Elsevier Science BV, Amsterdam Netherlands, pp. 95-116.

Walhagen, K., Unger, K., Hearn, M.T.W., 2000, Capillary electroendoosmotic chromatography of peptides, Journal of Chromatography A, vol 887, Elsevier Science BV, Amsterdam Netherlands, pp. 165-185.

O'Bryan, M.K., Sebire, K.L., Gerdprasert, O., Hedger, M., Hearn, M.T.W., de Kretser, D.M., 2000, Cloning and regulation of the rat activin betaE subunit, Journal of Molecular Endocrinology, vol 24, Society for Endocrinology, Bristol UK, pp. 409-418.

Graham, B., Grannas, M.J., Hearn, M.T.W., Kepert, C., Spiccia, L., Skelton, B.W., White, A.H., 2000, Coordination Chemistry of a Novel Tetramacrocyclic Ligand Derived from 1,4,7-Triazacyclononane: Synthesis, Structure, and Properties of Nickel(II) and Copper(II) Complexes, Inorganic Chemistry, vol 39, American Chemical Society, Washington DC USA, pp. 1092-1099.

Hearn, M.T.W., Keah, H., Boysen, R.I., Messana, I., Misiti, F., Rossetti, D.V., Giardina, B., Castagnola, M., 2000, Determination of biophysical parameters of polypeptide retro-inverso isomers and their analogues by capillary electrophoresis, Analytical Chemistry, vol 72 issue 9, American Chemical Society, Washington USA, pp. 1964-1972.

Boysen, R.I., Hearn, M.T., 2000, Direct characterisation by electrospray ionisation mass spectroscopy of mercuro-polypeptide complexes after deprotection of acetamidomethyl groups from protected cycteine residues of synthetic polypeptides, Journal of Biochemical and Biophysical Methods, vol 45, Elsevier Science BV, Amsterdam Netherlands, pp. 157-168.

Walhagen, K., Unger, K., Hearn, M.T.W., 2000, Influence of temperature on the behaviour of small linear peptides in capillary electrochromatography, Journal of Chromatography A, vol 893, Elsevier Science BV, Amsterdam Netherlands, pp. 401-409.

Li, S.K.B., Hearn, M.T.W., 2000, Isolation of thecal cells: and assessment of purity and steroidogenic potential, Journal of Biochemical and Biophysical Methods, vol 45, Elsevier Science BV, Amsterdam Netherlands, pp. 169-181.

Walhagen, K., Unger, K., Hearn, M.T.W., 2000, Life-time studies with capillary electrochromatography columns operated under different conditions, Journal of Chromatography A, vol 894, Elsevier Science BV, Amsterdam Netherlands, pp. 35-43.

Hearn, M.T.W., Gomme, P.T., 2000, Molecular architecture and biorecognition processes of the cystine knot protein superfamily: part I. The glycoprotein hormones, Journal of Molecular Recognition, vol 13, John Wiley & Sons, Chichester UK, pp. 223-278.

Kronina, V., Wirth, H.-., Hearn, M.T., 1999, Characterisation by immobilised metal ion affinity chromatographic procedures of the binding behaviour of several synthetic peptides designed to have high affinity for Cu(II) ions, Journal of Chromatography A, vol 852, Elsevier, Amsterdam Netherlands, pp. 261-272.

Purcell, A.W., Zhao, G., Aguilar, M., Hearn, M.T., 1999, Comparison between the isocratic gradient retention behaviour of polypeptides in reversed-phase liquid chromatographic environments, Journal of Chromatography A, vol 852, Elsevier, Amsterdam Netherlands, pp. 43-57.

Gomme, P.T., Stanton, P., Hearn, M.T., 1999, Evaluation of a pepscan approach to identify epitopes recognised by anti-hTSH monoclonal antibodies, J Biochemical & Biophysical Methods, vol . 38, Elsevier, Shannon Ireland, pp. 53-70.

Chaouk, H., Hearn, M.T., 1999, Examination of the protein behaviour of immobilised copper (II)-2,6-diaminomethylpyridine and its application in the immobilised metal ion affinity chromatographic separation of several human serum proteins, Biochemical & Biophysical Methods, vol 39, Elsevier, Shannon Ireland, pp. 161-177.

Gomme, P.T., Thompson, P.E., Whisstock, J.C., Stanton, P., Hearn, M.T., 1999, Identification of functional regions of the thyrotropin beta-subunit recognised by two monoclonal antibodies mAb<279 and mAb<299: a chimetic peptide approach, J Peptide Research, vol 54, Munksgaard International Publishers Ltd, Copenhagen Denmark, pp. 218-229.

Hearn, M.T., Zhao, G., 1999, Investigations into the thermodynamics of polypeptide interaction with nonpolar ligands, Analytical Chemistry, vol 71, no 21, American Chemical Society, Washington DC USA, pp. 4874-4885.

Chaouk, H., Hearn, M.T., 1999, New ligand N-(2-pyridylmethyl)aminoacetate, for use in the immobilised metal ion affinity chromatographic separation of proteins, Journal of Chromatography A, vol 852, Elseveir, Amsterdam Netherlands, pp. 105-115.

Boysen, R.I., Wang, Y., Keah, H., Hearn, M.T., 1999, Observations on the origin of the non-linear Van't Hoff behaviour of polypeptides in hydrophobic environments, Biophysical Chemistry, vol 77 issue 2-3, Elsevier, Amsterdam Netherlands, pp. 79-97.

Purcell, A.W., Aguilar, M., Hearn, M.T., 1999, Probing the binding behavior and conformational states of globular proteins in reversed-phase high-performance liquid chromatography, Analytical Chemistry, vol 71 no 3, American Chemical Society, Washington DC USA, pp. 2440-2451.

Walhagen, K., Unger, K., Olsen, A., Hearn, M.T., 1999, Separation of selected peptides by capillary electroendoosmotic chromatography using 3 um reversed-phase bonded silica and mixed-mode phases, Journal of Chromatography A, vol 853, Elsevier, Amsterdam Netherlands, pp. 263-275.

Sitaram, B.R., Keah, H., Hearn, M.T., 1999, Studies on the relationship between structure and electrophoretic mobility of alpha-helical and beta-sheet peptides using capillary zone electrophoresis, Journal of Chromatography A, vol 857, Elsevier Science BV, Amsterdam Netherlands, pp. 263-273.

Gomme, P.T., Thompson, P.E., Whisstock, J.C., Stanton, P., Hearn, M.T., 1999, Use of synthetic peptides to delineate discontinuous sequence regions involved in epitope sites of the thyrotropin beta-subunit, Letters in Peptide Science, vol 6, no 2-3, Kluwer Academic Publishers, Dordrecht Netherland, pp. 185-192.

McFarlane, J.R., Foulds, L.M., O'Connor, A.E., Phillips, D.J., Jenkin, G., Hearn, M.T., de Kretser, D.M., 1999, Uterine milk production, a novel activin-binding protein, is present in ovine allantoic fluid, Endocrinology, vol 140 issue 10, The Endocrine Society, Bethesda MD USA, pp. 4745-4752.

McFarlane, J.R., Foulds, L.M., O'Conner, A., Phillips, D.J., Jenkin, G., Hearn, M.T., de Kretser, D.M., 1999, Uterine milk protein, a novel Activin Binding Protein, is present in ovine allantoic fluid, Endocrinology, vol 140, The Endocrine Society, Bethesda USA, pp. 4745-4752.

McFarlane, J.R., Foulds, L.M., O'Connor, A.E., Phillips, D.J., Jenkin, G., Hearn, M.T., de Kretser, D.M., 1999, Uterine milk protein, a novel activin-binding protein, is present in ovine allantoic fluid, Endocrinology, vol 140, issue 10, Endocrine Society, Bethesda USA, pp. 4745-4752.

Keah, H., Kecorius, E., Hearn, M.T., 1998, Direct synthesis and characterisation of multi-dendritic peptides for use as immunogens, Journal of Peptide Research, vol 51 no 1, Munksgaard, Copenhagen Denmark, pp. 2-8.

Finette, G.M., Mao, Q., Hearn, M.T., 1998, Examination of Protein Adsorption in Fluidized Bed and Packed Bed Columns at Different Temperatures Using Frontal Chromatographic Methods, Biotechnology and Bioengineering, vol 58 no 1, John Wiley & Sons, UK, pp. 35-46.

O'Bryan, M.K., Loveland, K.L., Herszfeld, D., McFarlane, J.R., Hearn, M.T., de Kretser, D.M., 1998, Identification of a rat testis-specific gene encoding a potential rat outer dense fibre protein, MOLECULAR REPRODUCTION AND DEVELOPMENT, vol 50, WILEY-LISS, INC, New York NY USA, pp. 313-322.

O'Bryan, M.K., Loveland, K.L., Herszfeld, D., McFarlane, J.R., Hearn, M.T., de Kretser, D.M., 1998, Identification of a rat testis-specific gene encoding a potential rat outer dense fibre protein, Molecular Reproduction and Development, vol 50, Wiley-Liss Inc, West Sussex UK, pp. 313-322.

Jiang, W., Graham, B., Spiccia, L., Hearn, M.T., 1998, Protein selectivity with immobilized metal ion-tacn sorbents: Chromatographic studies with human serum proteins and several other globular proteins, Analytical Biochemistry, vol 255, Academic Press, USA, pp. 47-58.

Jiang, W., Graham, B., Spiccia, L., Hearn, M.T., 1998, Protein selectivity with immobilized metal ion-tacn sorbents: chromatographic studies with human serum proteins and several other globular proteins, Analytical Biochemistry, vol 225 no 1, Academic Press, San Diego USA, pp. 47-58.

Aguilar, M., Clayton, D.J., Holt, P., Kronina, V., Boysen, R.I., Purcell, A.W., Hearn, M.T., 1998, RP-HPLC binding domains of proteins, Analytical Chemistry, vol 70 no 23, American Chemical Society, Boston USA, pp. 5010-5018.

Cavallaro, V., Thompson, P.E., Hearn, M.T., 1998, Solid Phase Synthesis of Cyclic Peptides: Model Studies Involving 1 - (i+4) Side-Chain to Side-Chain Cyclisation, Journal of Peptide Science, vol 4, John Wiley & Sons, West Sussex UK, pp. 335-343.

Boysen, R.I., Erdmann, V.A., Hearn, M.T., 1998, Systematic, computer-assisted optimisation of the isolation of theremus thermophilus 50S ribosomal proteinsby reversed phase high performance liquid chromatography, Journal of Biochemical and Bioophysical Methods, vol 37, Elsevier Science B.V., Shannon Ireland, pp. 69-89.

Thompson, P.E., Steer, D., Aguilar, M., Hearn, M.T., 1998, Tropane-based amino acids for peptide structure-function studies: inhibitors of platelet aggregation, Bioorganic and Medicinal Chemistry Letters, vol 8 no 19, Elsevier Science Ltd, Oxford UK, pp. 2699-2704.

Finette, G.M., Baharin, B., Mao, Q., Hearn, M.T., 1997, Adsorption behaviour of multicomponent protein mixtures containing a1 - proteinase inhibitor with the anion exchanger, 2 -(Diethylamino)ethyl- Spherodex, Biotechnology Progress, vol 13, Amer. Chem. Soc. & the Amer. Inst. of Chem. Eng., Columbus OH USA, pp. 265-275.

Cokugras, A., Stanton, P., Hearn, M.T., 1997, Analysis of 9-fluorenylmethyl chloroformate derivatives of amino sugars by capillary electrophoresis with UV detection, Turkish Journal of Biochemistry, vol 22, Turkish Biochemical Society, Ankara Turkey, pp. 9-12.

Bjorklund, M., Hearn, M.T., 1997, Characterisation of silica-based heparin affinity sorbents from equilibrium binding studies on plasma fractions containing thrombin, Journal of Chromatography A, vol 762, Elsevier Science B.V., Amsterdam Netherlands, pp. 113-134.

Zachariou, M., Traverso, I., Spiccia, L., Hearn, M.T., 1997, Characterization by potentiometric procedures of the acid-base and metal binding properties of two new classes of immobilized metal ion affinity adsorbents developed for protein purification, Analytical Chemistry, vol 69, American Chemical Society, Columbus OH USA, pp. 813-822.

Finette, G.M., Mao, Q., Hearn, M.T., 1997, Comparative studies on the isothermal characteristics of proteins adsorbed under batch equilibrium conditions to ion-exchange, immobilised metal ion affinity and dye affinity matrices with different ionic strength and temperature conditions, Journal of Chromatography A, vol 763, Elsevier Science B.V., Amsterdam Netherlands, pp. 71-90.

Higgins, K., Bicknell, W., Keah, H., Hearn, M.T., 1997, Comparison of the solution conformations of a human immunodeficiency virus peptidomimetic and its retro-inverso isomer using 'H NMR spectroscopy, Journal of Peptide Research, vol 50, Munksgaard International Publishers, Copenhagen Denmark, pp. 421-435.

Higgins, K.A., Bicknell, W., Keah, H., Hearn, M.T.W., 1997, Comparison of the solution conformations of a human immunodeficiency virus peptidomimetic and its retro-inversoisomer 1H NMR spectroscopy, Journal of Peptide Research, vol 50, Munksgaard, Denmark, pp. 421-435.

Lazoura, E., Maidonis, I., Bayer, E., Hearn, M.T., Aguilar, M., 1997, Conformational analysis of neuropeptide Y-[18-36] analogs in hydrophobic environments, Biophysical Journal, vol 72, Biophysical Society, Bethesda USA, pp. 238-246.

Lee, T., Thompson, P.E., Hearn, M.T., Aguilar, M., 1997, Conformational stability of a type IIb- turn motif in human growth hormone [6 - 13] peptide analogues at hydrophobic surfaces, Journal of Peptide Research, vol 49, Munksgaard International Publishers Ltd., Copenhagen Denmark, pp. 394-403.

Chaouk, H., Middleton, S., Jackson, R., Hearn, M.T., 1997, Constrained immobilised metal ion chelate affinity adsorbents for protein purification, International Journal of Bio-Chromatography, vol 2, Harwood Academic Publishers, Amsterdam Netherlands, pp. 153-190.

Graham, B., Fallon, G.D., Hearn, M.T., Hockless, D.C.R., Lazarev, G., Spiccia, L., 1997, Coordination modes of a series of xylylene-bridged bis(1,4,7-triazacyclonon-1-yl) ligands: Synthesis, structure and properties of nickel(II) and copper(II) complexes, Inorganic Chemistry, vol 36, American Chemical Society, USA, pp. 6366-6373.

Graham, B., Fallon, G.D., Hearn, M.T., Hockless, D.C.R., Lazarev, G., Spiccia, L., 1997, Coordination modes of a series of xylylene-bridged bis(1,4,7-triazacyclonon-1-yl) ligands: synthesis, structure, and properties of nickel(II) and copper(II) complexes, Journal of Inorganic Chemistry, vol 36, The American Chemical Society, Washington DC USA, pp. 6366-6373.

Thompson, P.E., Hearn, M.T., 1997, Fmoc-protected tropane-based amino acids for peptide structure-function studies, Tetrahedron Letts, vol 38, Elsevier Science Ltd., Oxford UK, pp. 2907-2910.

Phillips, D.J., McFarlane, J.R., Hearn, M.T., de Kretser, D.M., 1997, Inhibin, activin and follistatin bind preferentially to the transformed species of a2 - macroglobulin, Journal of Endocrinology, vol 155, Journal of Endocrinology Ltd., Bristol UK, pp. 65-71.

Phillips, D.J., McFarlane, J.R., Hearn, M.T., de Kretser, D.M., 1997, Inhibin, activin and follistatin bind preferentially to the transformed sprecies of alpha two - macroglobulin, Journal of Endocrinology, vol 155, Society for Endocrinology, Colchester UK, pp. 65-71.

Kim, Y.H., de Kretser, D.M., Temple-Smith, P.D., Hearn, M.T., McFarlane, J.R., 1997, Isolation and characterization of human and rabbit sperm tail fibrous sheath, Molecular Human Reproduction, vol 3, Oxford University Press, Oxford UK, pp. 307-313.

Yarovsky, I., Hearn, M.T., Aguilar, M., 1997, Molecular simulation of peptide interactions with an RP-HPLC sorbent, The Journal of Physical Chemistry B, vol 101, American Chemical Society, Columbus OH USA, pp. 10962-10970.

Unger, K., Myers, P., Hearn, M.T., 1997, Standardized reversed-phase materials: myth or reality?, American Laboratory, vol 29, International Scientific Communications Inc., Shelton CT USA, pp. 22-28.

Jensen, W., Armstrong, J.M., Giorgio, J., Hearn, M.T., 1997, Thermodynamic analysis of the stabilisation of pig heart mitochondrial malate dehydrogenase and maize leaf phosphoenolpyruvate carboxylase by different salts, amino acids and polyols, Biochimica et Biophysica Acta, vol 1338, Elsevier Science B.V., Amsterdam Netherlands, pp. 186-198.

Spiccia, L., Graham, B., Hearn, M.T., Lazarev, G., Moubaraki, B., Murray, K.S., Tiekink, E.R.T., 1997, Towards synthetic models for trinuclear copper active sites of ascorbate oxidase and laccase: self-assembly, crystal structure and magnetic properties of the copper (II) complexes of 1,3,5-tris(1,4,7-triazacyclonon-1-ylmethyl)benzene, Journal of the Chemical Society-Dalton Transactions, vol -, Royal Society of Chemistry, Cambridge UK, pp. 4089-4097.

Spiccia, L., Graham, B., Hearn, M.T., Lazarev, G., Moubaraki, B., Murray, K.S., Tiekink, E.R.T., 1997, Towards synthetic models for trinuclear copper active sites of ascorbate oxidase and laccase: self-assembly, crystal structure and magnetic properties of the copper(II) complexes of 1,3,5-tris(1,4,7-triazacyclonon-1-ylmethyl)benzene, Journal of the Chemical Society, Dalton Transactions, vol no, Royal Society of Chemistry, UK, pp. 4089-4097.

Conference Proceedings

Do Pham, D., Kelso, G., Yang, Y., Hearn, M., 2011, Investigation of FeIII-tamil catalyst in oxidative organic synthesis, Green Chemistry 2011 Innovations, 4-7 December, Royal Australian Chemistry Institute, Australia.

Nguyen, M., Chaffee, A.L., Held, M., Boysen, R.I., Nicolau, D., Hearn, M.T., 2008, A study on the atomic hydrophobicity of peptides in aqueous solutions using molecular dynamics modeling methods, Biomedical Applications of Micro- and Nanoengineering IV and Complex Systems, 10 December 2008 to 12 December 2008, SPIE - International Society for Optical Engineering, United States, pp. 727002-1-727002-10.

Komaromy, A., Boysen, R., Zhang, H., Hearn, M., Nicolau, D., 2008, Influence of surface nanostructure on the extent of colonization and cell viability of E. coli and S. aureus, Biomedical Applications of Micro- and Nanoengineering IV and Complex Systems, 09 December 2008 to 12 December 2008, SPIE The International Society Optical Engineering, USA, pp. 727006-1-727006-8.

Li, S., Komaromy, A., Boysen, R., Nicolau, D., Hearn, M., 2008, Self-assembled diblock copolymer thin films for the analysis of bacteria-surface interactions, Biomedical Applications of Micro- and Nanoengineering IV and Complex Systems, 09 December 2008 to 12 December 2008, SPIE The International Society Optical Engineering, USA, pp. 727008-1-727008-8.

Zhang, H., Boysen, R., Sune, G., Borrise, X., Perez-Murano, F., Hearn, M., Nicolau, D., 2008, The effect of hydrophobicity of micro/nanostructured-surfaces on behaviours of water spreading, Biomedical applications of micro- and nanoengineering IV and complex systems, 09 December 2008 to 12 December 2008, SPIE The International Society Optical Engineering, USA, pp. 727009-1-727009-8.

Zhang, H., Komaromy, A.z., Boysen, R.I., Rius, G., Borrise, X., Perez-Murano, F., Hearn, M.T.W., Nicolau, D.V., 2007, Atomic force microscopy study on the attachment of E. coli and S. aureus to a patterned surface of different materials, Proceedings of SPIE: BioMEMS and Nanotechnology III, 04/12/2007-07/12/2007, SPIE, USA, pp. 679903-679912.

Komaromy, A.z., Boysen, R.I., Zhang, H., Hearn, M.T.W., Nicolau, D.V., 2007, Effect of various artificial surfaces on the colonization and viability of E. coli and S. aureus, Proceedings of SPIE, BioMEMS and Nanotechnology III, 04/12/2007-07/12/2007, SPIE, USA, pp. 67990-67999.

Keah, H., Kecorius, E., Hearn, M.T., 1998, Direct synthesis and characterisation of dendritic peptides, 3rd Australian Peptide Conference "From Discovery to Therapeutics", Laguna Quays The Whitsundays QLD Oct 4-9, 1998, Pink Panther Printing, Melbourne Vic Australia, p. 42.

Hearn, M.T., Boysen, R.I., Wang, Y., 1998, Entropy-enthalpy compensation effects in the molecular chromatography of polypeptide hormones in lipophilic environments, 18th International Symposium on Separation of Proteins, Peptides & Polynucleotides, Vienna Austria, Nov 1-4, 1998, http://www.boku.ac.at/iam/isppp98/, Vienna Austria, p. 37.

Hearn, M.T., Boysen, R.I., Wang, Y., 1998, Entropy-enthalpy compensation effects in the molecular chromatography of synthetic polypeptide hormones, 3rd Australian Peptide Conference "From Discovery to Therapeutics", Laguna Quays The Whitsundays QLD Oct. 4-9, 1998, Pink Panther Printing, Melbourne Vic Australia, p. 74.

Cheng, J.T., Hearn, M.T., Neale, A.D., 1998, Expression of novel HIV vaccine in plants, 6th Pacific Rim Biotechnology Conference and BioExpo '98, Hong Kong, 3-5 June, 1998, Hong Kong University of Science and Technology H K, Hong Kong, p. 233.

Johnson, A., Oddie, G., Mao, Q., Hearn, M.T., 1998, Modelling of the adsorption process of proteins with preparative ion exchange sorbents, 215th ACS National Meeting, Dallas U S A March 29 - April 2, 1998, American Chemical Society, Columbus USA, p. No.142.

Hearn, M.T., Boysen, R.I., Muraledaram, M., Jong, A., 1998, On the binding behaviour and conformational properties of Polypeptides and gobular proteins in hydrophobic environments, 18th International Symposium on Separation of Proteins, Peptides & Polynucleotides, Vienna Austria Nov 1-4, 1998, COME IN Congress-Meeting-Incentive Organisation, Vienna Austria.

Hearn, M.T., Boysen, R.I., Muralahajan, M., Jong, A., 1998, On the binding behaviour and conformational properties of polypeptides and globular proteins with non-polar ligates, 3rd Australian Peptide Conference "From Discovery to Therapeutics", Laguna Quays The Whitsundays QLD Oct 4-9, 1998, Pink Panther Printing, Melbourne Vic Australia, p. 74.

Hearn, M.T., Boysen, R.I., Wang, Y., 1998, On the binding behaviour and conformational properties of polypeptides and globular proteins with non-polar ligates, 3rd Australian Peptide Conference "From Discovery to Therapeutics", Laguna Quays Queensland, 4-9 October 1998, Pink Panther Printing, Melbourne Vic Australia, p. 74.

Hearn, M.T., Boysen, R.I., Wang, Y., Higgins, K., Keah, H., 1998, Polypeptide topomers: biophysical studies on their conformational and binding behaviour in hydrophobic environments, 3rd Australian Peptide Conference "From Discovery to Therapeutics", Laguna Quays The Whitsundays QLD Oct 4-9, 1998, Pink Panther Printing, Melbourne Vic Australia, p. 95.

Hearn, M.T., 1998, Pushing the Analytical Limits: New Biomedical and Pharmaceutical Outcomes from Recent Advances in the Analysis of Peptides and Proteins, Drug Analysis '98, Brussels Belgium, May 11-15, 1998, The Belgian Society of Pharmaceutical Sciences, Brussels Belgium, p. 115.

O'Bryan, M.K., Hearn, M.T., de Kretser, D.M., 1998, Sperm tail genes and infertility, The Australian Society For Medical Research, HOBART, 22.11.98, LILLY AUSTRALIA, HTTP://WWW.LILLY.COM.AU, p. 39.

Steer, D., Aguilar, M., Hearn, M.T., Thompson, P.E., 1998, Synthesis and evaluation of Fmoc protected tropane based amino acids and their Incorporation into peptides as fibrinogen peptidomimetics, Proceedings, 23rd Lorne Annual Conference on Protein Structure and Function, Lorne VIC, 8-12 February 1998, Lorne Protein Workshop Inc, Melbourne Vic Australia, p. B40.

Keah, H., O'Bryan, M.K., de Kretser, D.M., Hearn, M.T., 1998, The use of synthetic peptides in the study of sperm tail development function, 3rd Australian Peptide Conference "From Discovery to Therapeutics", Laguna Quays The Whitsundays QLD Oct 4-9, 1998, Pink Panther Printing, Melbourne Vic Australia, p. 43.

Hearn, M.T., Boysen, R.I., Muraladaran, M., Wang, Y., 1998, Thermodynamic and extra-thermodynamic dependencies in the retention behaviors of polypeptides in reversed phase HPLC, 22nd International Symposium on High Performance Liquid Phase Separations and Related Techniques, St Louis U S A, May 2-8, 1998, Barr Enterprises, Walkersville Maryland USA, p. 43.

O'Bryan, M.K., Keah, H., Hearn, M.T., de Kretser, D.M., 1998, Tpx-1 an outer dense fibre encoding gene in humans and rats, FSA'98 Program, Abstracts And Mini-Posters, HOBART, AUSTRALIA 28.10.98, The Fertility Society of Australia, Canberra ACT Australia, p. 25.

Thompson, P.E., Higgins, K., Hearn, M.T., 1997, Application of tropane-based peptidomimetics, Proceedings, 15th American Peptide Symposium, Nashville, TN, USA, June 14-19, 1997, American Peptide Society, Nashville TN USA, p. 2-74.

Hearn, M.T., Boysen, R.I., Muralahajan, M., 1997, Capillary electrophoresis as a tool to assess the conformational status and ligand binding behaviour of peptides and proteins: theoretical and practical considerations, 10th International Symposium on High Performance Capillary Electrophoresis, Kyoto, Japan, July 8-11, 1997, Elsevier Science B.V., Amsterdam Netherlands, pp. 53-54.

Round, A.J., Hearn, M.T., Aguilar, M., 1997, Capillary electrophoresis of peptides: effects of conformation and structural isomerism in micellar environments, Proceedings, 22nd Annual Lorne Conference on Protein Structure and Function, Lorne, Australia, Feb.10-14, 1997, Lorne Protein Workshop Inc., Melbourne Vic Australia, p. C25.

Hearn, M.T., 1997, Combinatorial ligands in high resolution bioseparation: the next generation of selectivity opportunities for the analysis & purification of biological molecules in pseudo-affinity interaction modes or a pandora's box?, 17th International Symposium on the Separation of Proteins, Peptides and Polynucleotides, Rockvile, Maryland USA, Oct. 26-29, 1997, Barr Enterprises, Rockville MD USA, p. 31.

Bjorklund, M., Finette, G.M., Oddie, G., Mao, Q., Hearn, M.T., 1997, Comparative performance of packed and expanded beds with ion-exchange and affinity adsorbents for the purification of proteins: assessment of several key operational parameters during scale-up, Proceedings , 21st International Symposium on High Performance Liquid Chromatography, Birmingham, England, June 22-27, 1997, HPLC 97, Bradford UK, p. 54.

Kronina, V., Wirth, H.-., Hearn, M.T., 1997, Design of peptide sequences with high affinity for immobilised Cu(II) ions, Proceedings , 21st International Symposium on High Performance Liquid Chromatography, Birmingham, England, June 22-27, 1997, HPLC 97, Bradford UK, p. 1.

Wei, J., Graham, B., Wirth, H.-., Boysen, R.I., Spiccia, L., Hearn, M.T., 1997, Engineering ligands for the high resolution analysis and purification of recombinant proteins, 17th International Symposium on the Separation of Proteins, Peptides and Polynucleotides, Rockvile, Maryland USA, Oct. 26-29, 1997, Barr Enterprises, Rockville MD USA, p. 41.

Gomme, P.T., Thompson, P.E., Whisstock, J.C., Hearn, M.T., 1997, Homology modelling of the glycoprotein hormones: receptor recognition characteristics, 1st International Peptide Symposium, Kyoto, Japan, Nov. 30-Dec. 5, 1997, Kluwer Academic Publishers B.V., Amsterdam Netherlands, p. 122.

Gomme, P.T., Fairlie, W., Thompson, P.E., Stanton, P., Hearn, M.T., 1997, Identification of the epitope site associated with receptor recognition on the thyrotropin b-subunit, Proceedings, 22nd Annual Lorne Conference on Protein Structure and Function, Lorne, Australia, Feb. 9-13, 1997, Lorne Protein Workshop Inc., Melbourne Vic Australia, p. B36.

Clay, R., Boysen, R.I., Hearn, M.T., 1997, Investigation of the a2 - macroglobulin binding regions of Activin bA, bB, bC and bD variants using synthetic peptides, Proceedings, 22nd Annual Lorne Conference on Protein Structure and Function, Lorne, Australia, Feb. 9-13, 1997, Lorne Protein Workshop Inc., Melbourne Vic Australia, p. B54.

Hearn, M.T., Boysen, R.I., Muralahajan, M., Wang, Y., 1997, Investigations into the origin of the deviations from ideal retention behavior of peptides with n-Alkysilica sorbents, 17th International Symposium on the Separation of Proteins, Peptides and Polynucleotides, Rockville, Maryland, USA, Oct. 26-29, 1997, Barr Enterprises, Rockville MD USA, p. 59.

Hearn, M.T., 1997, New opportunities for the structural characterization of peptides and proteins by RP-HPLC: from amino acid sequence determination to tertiary structure assessment, Biosep '97 XI Proficiency Course on the Chromatographic and Electrophoretic Techniques, Rome, Italy, April 8-11, 1997, Italian Chemical Society, Rome Italy, pp. 1H-23H.

Hearn, M.T., Boysen, R.I., Wang, Y., Muraledaran, M., 1997, Peptide conformational behaviour studied by high and low temperature HPLC and HPCE, 1st International Peptide Symposium, Kyoto, Japan, Nov. 30-Dec. 5, 1997, Kluwer Academic Publishers B.V., Amsterdam Netherlands, p. 67.

Cavallaro, V., Thompson, P.E., Stanton, P., Hearn, M.T., 1997, Peptides with affinity for insulin, Proceedings, 22nd Annual Lorne Conference on Protein Structure and Function, Lorne, Australia, Feb. 9-13, 1997, Lorne Protein Workshop Inc., Melbourne Vic Australia, p. A73.

Hearn, M.T., 1997, Recent advances in biomimetic affinity chromatography of proteins, The 3rd Symposium on Trends in Biotechnology & 7th Scientific Meeting of MSMBB, Serdang Selangor Malaysia, May 15-17, 1997, University Putra, Faculty of Food Science, Serdang Malaysia, p. 73.

Wei, J., Hearn, M.T., 1997, Separation of human serum protein with immobilised metal ion-tacn and its derivatives absorbents, 17th International Symposium on the Separation of Proteins, Peptides and Polynucleotides, Rockvile, Maryland USA, Oct. 26-29, 1997, Barr Enterprises, Rockville MD USA, p. 41.

Boysen, R.I., Keah, H., Wang, Y., Hearn, M.T., 1997, Studies on the retention behaviour and conformational stability of all L-a- and their retro - inversion all D-a - peptide isomer pairs using reversed - phase high - performance liquid chromatography, Proceedings, 22nd Annual Lorne Conference on Protein Structure and Function, Lorne, Australia, Feb. 9-13, 1997, Lorne Protein Workshop Inc., Melbourne Vic Australia, p. A88.

Steer, D., Aguilar, M., Hearn, M.T., Thompson, P.E., 1997, Synthesis of FMOC protected tropane based amino acids and their incorporation into fibrinogen related peptides, Proceedings of the Australian Society for Biochemistry and Molecular Biology, Melbourne, Sept. 29-Oct. 2, 1997, Australian Soc. for Biochemistry & Molecular Biol., Kent Town SA, p. B1-87.

Thompson, P.E., Steer, D., Higgins, K., Aguilar, M., Hearn, M.T., 1997, Synthesis, conformational analysis and application of tropane-based FMOC-amino acid derivatives: novel antagonists of fibrinogen receptor, GPIIb/IIIa, 1st International Peptide Symposium, Kyoto, Japan, Nov. 30-Dec. 5, 1997, Kluwer Academic Publishers B.V., Amsterdam Netherlands, p. 105.

O'Bryan, M.K., Loveland, K.L., Herszfeld, D., McFarlane, J.R., Hearn, M.T., de Kretser, D.M., 1997, Tpx-1: A Rat Outer Dense Fibre Protein Gene, Aust Society for Reproductive Biology program and miniposters of abstracts, 29/9-1/10/97 Canberra, Vertebrate Biocontrol Cooperative Research Centre, Canberra ACT Australia, p. 27.

Other

Hearn, M., Langford, S., Tuck, K., Harris, S., Boysen, R., Perchyonok, V., Danylec, B., Schwarz, L., Chowdhury, J., 2010, Moleculary Imprinted Polymers, Melbourne, Victoria, Australia.

Maharjan, P., De Silva, K., Woonton, B., Hearn, M., Jackson, W., 2010, Temperature-responsive polymer particles in protein separation applications, Australia.

Graham, B., Mooney, J., Jiang, W., Hearn, M., Christensen, T., Spiccia, L., 2009, Peptide purification by means of metal ion affinity chromatography, Japan.

Christensen, T., Hearn, M.T.W., Spiccia, L., Jiang, W., Mooney, J.T., Graham, B., 2008, Peptide purification by means of metal ion affinity chromatography.

Alam, M.A., Bowden, D.K., Boysen, R.I., Hearn, M.T.W., 2005, A method for the rapid analysis of polypeptides, Australia.

Boysen, R.I., Hearn, M.T.W., 2003, Desalting of peptides and protein mixtures by RP-HPLC techniques, Proteins and Proteomics: A laboratory manual, Simpson, R. (ed.), Chapter 5, Reversed-phase high-performance liquid chromatography, Protocol 5, Cold Spring Harbor Laboratory Press, USA.

Christensen, T., Hearn, M.T.W., Spiccia, L., Jiang, W., Mooney, J.T., 2003, Peptide Purification by Means of Metal Ion Affinity Chromatography, Worldwide.

Christensen, T., Hearn, M.T.W., Spiccia, L., Jiang, W., Mooney, J.T., Graham, B., 2003, Peptide Purification by Means of Metal Ion Affinity Chromatography, Europe.

Boysen, R.I., Hearn, M.T.W., 2003, Purification of peptides from solid phase peptide synthesis with reversed-phase high-performance liquid chromatography, Proteins and Proteomics: A laboratory manual, Simpson, R. (ed.), Chapter 5, Reversed-phase high-performance liquid chromatography, Protocol 4, Cold Spring Harbor Laboratory Press, USA.

Boysen, R.I., Hearn, M.T.W., 2003, Separation of proteins with reversed-phase high-performance liquid chromatography using a computer assisted method to optimise gradient conditions, Proteins and Proteomics: A laboratory manual, Simpson, R. (ed.), Chapter 5, Reversed-phase high-performance liquid chromatography, Protocol 6, Cold Spring Harbor Laboratory Press, USA.

Graham, B., Mooney, J., Jiang, W., Hearn, M., Christensen, T., Spiccia, L., 2002, Peptide purification by means of metal ion affinity chromatography, Sydney, NSW, Australia.

Krsmanovic, V., Cosic, I., Biquard, J., Hearn, M.T., 1998, Analgous peptides of the internal image of a viral protein, Patented in Australia (applied for Euro USA Jap).

Graham, B., Grannas, M.J., Spiccia, L., Fallon, G.D., Hearn, M.T., Tiekink, E.R.T., Hockless, D.C.R., Skelton, B.W., White, A.H., 1997, Synthesis and application of polynuclear metal complexes in immobilised metal affinity chromatography (IMAC), RACI 3rd Annual Postgraduate Symposium on Inorganic Chemistry, RACI, Australia.

Awards

Academic awards (national, international)

2012
Leighton Memorial Medal
Royal Australian Chemical Institute

Postgraduate Research Supervisions

Current Supervision

Program of Study:: (DOCTORATE BY RESEARCH).
Thesis Title:: Advanced analysis of polyphenols and phytosterols in grapes and grape by products.
Supervisors:: Boysen, R (Joint), Hearn, M (Joint-co).

Program of Study:: (DOCTORATE BY RESEARCH).
Thesis Title:: Bioinspired nano micelles: Their controlled chemical loading and release..
Supervisors:: Saito, K (Main), Hearn, M (Associate), Bell, T (Associate).

Program of Study:: (DOCTORATE BY RESEARCH).
Thesis Title:: Capillary electrochromatography (CEC) of polar compounds: Towards an improved understanding of the fundamentals of retention and separation efficiency..
Supervisors:: Hearn, M (Joint-co), Saito, K (Joint).

Program of Study:: (DOCTORATE BY RESEARCH).
Thesis Title:: Capillary electrophoresis separation and high-throughput proteomics analysis of recombinant proteins.
Supervisors:: Hearn, M (Main), Boysen, R (Associate), Harris, S (Associate).

Program of Study:: (DOCTORATE BY RESEARCH).
Thesis Title:: Comparison of different smart polymers for natural product separation.
Supervisors:: Saito, K (Main), Boysen, R (Associate), Hearn, M (Associate).

Program of Study:: (DOCTORATE BY RESEARCH).
Thesis Title:: Investigation of Butyrolactone like Small Molecules in Regulation of Antibiotic Production in Streptomyces.
Supervisors:: Boysen, R (Joint), Hearn, M (Joint-co).

Program of Study:: (DOCTORATE BY RESEARCH).
Thesis Title:: Investigation of protein-ligand interactions in liquid chromatographic systems.
Supervisors:: Boysen, R (Joint-co), Hearn, M (Joint).

Program of Study:: (DOCTORATE BY RESEARCH).
Thesis Title:: One-Pot Oxidative N-Demethylation of Tropane Alkaloids with Hydrogen Peroxide and Fe III - TAML Catalyst.
Supervisors:: Hearn, M (Joint-co), Kelso, G (Joint).

Program of Study:: (DOCTORATE BY RESEARCH).
Thesis Title:: Optimising enzymes and developing novel enzyme-catalysed reaction conditions for the hydrolysis of cellulose and xylan.
Supervisors:: Hearn, M (Joint-co), Zhang, C (Joint).

Program of Study:: (DOCTORATE BY RESEARCH).
Thesis Title:: Study on the flavonoid of wine.
Supervisors:: Marriott, P (Main), Hearn, M (Associate).

Program of Study:: (DOCTORATE BY RESEARCH).
Thesis Title:: Substituted terpyridines: Green synthetic strategies and application in antibody purification..
Supervisors:: Hearn, M (Joint), Saito, K (Joint-co).

Completed Supervision

Student:: Acosta, D.
Program of Study:: A novel TGF-B superfamily member: Expression of recombinant human activin C in yeast, Pichia pastoris.. (Masters) 2006.
Supervisors:: Hearn, M (Main).
Student:: Alam, A.
Program of Study:: High resolution mass spectrometry based analysis of biological samples. (PHD) 2008.
Supervisors:: Bowden, D (Main), Hearn, M (Associate), Boysen, R (Associate).
Student:: Allen, N.
Program of Study:: SYNTHESIS AND STUDIES ON THE THERMODYNAMIC, CONFORMATIONAL AND INTERACTIVE PROPERTIES OF ACTIVIN BA - BD LOOP PEPTIDES. (PHD) 2002.
Supervisors:: Hearn, M (Main).
Student:: Banti, Y.
Program of Study:: Novel molecularly imprinted polymers (MIPs) mimicking the catalytic activity of trypsin. (PHD) 2008.
Supervisors:: Hearn, M (Main), Boysen, R (Associate).
Student:: Bhagwat, A.
Program of Study:: Nitrogen heterocycles: novel tools in protein purification. (PHD) 2010.
Supervisors:: Jackson, W (Main), Hearn, M (Associate).
Student:: Chen, J.
Program of Study:: Analysis of glycosylated biomolecules. (PHD) 2006.
Supervisors:: Hearn, M (Main), Boysen, R (Associate).
Student:: Chen, J.
Program of Study:: Studies in protein interactions. 2001.
Supervisors:: Hearn, M (Main).
Student:: Ciayadi, R.
Program of Study:: The synthesis of inhibitors of TGF-beta cytokine signalling and identification of their protein binding partners. (PHD) 2012.
Supervisors:: Hearn, M (Main), Harris, S (Associate), Kelso, G (Associate).
Student:: Coghlan, C.
Program of Study:: Green chemical solutions for the separation of recombinant proteins, including biopharmaceuticals. (PHD) 2011.
Supervisors:: Hearn, M (Joint-Co), Jackson, W (Joint).
Student:: Daly, R.
Program of Study:: EXPRESSION AND CHARACTERISATION OF THE LIGAND-BINDING DOMAINS OF ACTIVIN RECEPTORS. (PHD) 2002.
Supervisors:: Hearn, M (Main), Spiccia, L (Associate).
Student:: Fadhel, B.
Program of Study:: CO2 adsorption by ordered mesoporous substrates modified with amine-terminated dendrimers. (PHD) 2009.
Supervisors:: Chaffee, A (Joint), Hearn, M (Joint-Co).
Student:: Huth, S.
Program of Study:: Amino acid derivatives of 1,4,7-triazacyclononane: synthesis, properties and their use in the preparation of peptides with metal-binding properties. (PHD) 2009.
Supervisors:: Hearn, M (Joint), Spiccia, L (Joint-Co).
Student:: Jiang, W.
Program of Study:: NOVEL IMMOBILISED METAL ION AFFINITY CHROMATOGRAPHIC ADSORBENTS FOR PROTEIN SEPARATION AND PURIFICATION . (PHD) 2000.
Supervisors:: Hearn, M (Main).
Student:: Jong, A.
Program of Study:: THERMODYNAMIC ANALYSIS OF THE INTERACTION OF ALPHA-HELICAL POLYPEPTIDES AND PROTEINS WITH NONPOLAR LIGANDS IN REVERSED-PHASE HPLC. (PHD) 2003.
Supervisors:: Hearn, M (Main), Boysen, R (Associate).
Student:: Kreher, U.
Program of Study:: Hard metal ion chelating complexes for the application in immobilised metal affinity chromatography (IMAC) of biomolecules. (PHD) 2006.
Supervisors:: Hearn, M (Joint), Spiccia, L (Joint-Co).
Student:: Longford, D.
Program of Study:: Studies with chimeric protein purification methods. (PHD) 2011.
Supervisors:: Hearn, M (Main), Daly, R (Associate).
Student:: Mataj, A.
Program of Study:: A new approach to synthesis and application of novel classes of ligands for the affinity capture and characterisation of phosphorylated peptides. (PHD) 2010.
Supervisors:: Hearn, M (Main), Boysen, R (Associate).
Student:: Mooney, J.
Program of Study:: Development of a novel IMAC system using the MCL tacn. (PHD) 2006.
Supervisors:: Hearn, M (Main).
Student:: Mountford, S.
Program of Study:: Preparation of novel ligands for antibody purification. (PHD) 2006.
Supervisors:: Hearn, M (Joint-Co), Robinson, A (Joint).
Student:: Oktavianawati, I.
Program of Study:: Xylanase-Hydrolysed Reaction on Xylan in Ionic Liquids. (Masters) 2008.
Supervisors:: Hearn, M (Main), Zhang, C (Associate).
Student:: Pang, A.
Program of Study:: . 2002.
Supervisors:: Hearn, M (Main).
Student:: Pang, A.
Program of Study:: EXPRESSION, PURIFICATION AND CHARACTERISATION OF HUMAN RECOMBINANT ACTIVIN A. (Masters) 2004.
Supervisors:: Hearn, M (Main).
Student:: Petzold, M.
Program of Study:: Novel small molecule ligand affinity chromatography systems: high-throughput screening and scale-up. (PHD) 2011.
Supervisors:: Hearn, M (Main).
Student:: Pinczewski, J.
Program of Study:: STUDIES WITH IMMUNOGENIC PEPTIDES RELATED TO HIV-1. (PHD) 2000.
Supervisors:: Hearn, M (Main).
Student:: Wang, G.
Program of Study:: "New approaches to purifying recombinant heterodimeric proteins: Activin AC expressed in Pichia pastoris". (Masters) 2006.
Supervisors:: Hearn, M (Main).
Student:: Wang, G.
Program of Study:: . 2004.
Supervisors:: Hearn, M (Main), Fredericks, D (Associate).
Student:: Wang, Y.
Program of Study:: INVESTIGATIONS INTO PROTEIN CONFORMATIONAL CHANGES IN HYDROPHOBIC INTERACTION ENVIRONMENTS. (PHD) 2003.
Supervisors:: Hearn, M (Main), Hearn, M (Main), Boysen, R (Associate).
Student:: Warden, A.
Program of Study:: COPPER(II) CO-ORDINATION CHEMISTRY AND ANION BINDING PROPERTIES OF POLYAZAMACROCYCLES. (PHD) 2004.
Supervisors:: Spiccia, L (Main), Hearn, M (Associate).
Student:: Widakowich, G.
Program of Study:: Studies in immobilized metal ion affinity chromatography of proteins. (PHD) 2010.
Supervisors:: Hearn, M (Main).
Student:: Yang, Y.
Program of Study:: A new strategy to achieve high resolution separation of peptides with novel capillary electrophoretic and capillary electrochromatographic systems. (PHD) 2005.
Supervisors:: Hearn, M (Main).
Student:: Yin, Y.
Program of Study:: Experimental investigation into biomolecule-ligand interaction. (PHD) 2007.
Supervisors:: Hearn, M (Main), Boysen, R (Associate).
Student:: Young, J.
Program of Study:: Synthesis of novel morphine analogues for medicinal applications. (PHD) 2006.
Supervisors:: Hearn, M (Joint-Co), Jackson, W (Joint).