Dr John Haynes - Researcher Profile

Biography

Position

Senior Lecturer
Faculty of Pharmacy and Pharmaceutical Sciences

Stem Cell Biology group leader
Medicinal Chemistry and Drug Action
Monash Institute of Pharmaceutical Sciences

Qualifications

GRADUATE CERTIFICATE IN HIGHER EDUCATION

Institution: Monash University

Year awarded: 2005

PHARMACOLOGY

Institution: Monash University

Year awarded: 1992

BSC (HONS)

Institution: Monash University

Year awarded: 1987

Publications

Journal Articles

Watmuff, B., Pouton, C.W., Haynes, J.M., 2012, In vitro maturation of dopaminergic neurons derived from mouse embryonic stem cells: Implications for transplantation, PLoS ONE [P], vol 7, issue 2, Public Library of Science, USA, pp. 1-11.

Nefzger, C., Su, C.T.E., Fabb, S.A., Hartley, B.J., Zeng, W., Haynes, J.M., Pouton, C.W., 2012, Lmx1a allows context-specific isolation of progenitors of GABAergic or dopaminergic neurons during neural differentiation of embryonic stem cells, Stem Cells [P], vol 30, issue 7, Wiley-Blackwell, USA, pp. 1349-1361.

Gosling, J.I., Baker, S.P., Haynes, J.M., Kassiou, M., Pouton, C.W., Warfe, L., White, P.J., Scammells, P.J., 2012, Synthesis and biological evaluation of adenosines with heterobicyclic and polycyclic N6-substituents as adenosine A1 receptor agonists, Chemmedchem [P], vol 7, issue 7, Wiley-V C H Verlag GMBH, Germany, pp. 1191-1201.

Hausler, N., Devine, S., McRobb, F.M., Warfe, L., Pouton, C.W., Haynes, J.M., Bottle S.E., White, P.J., Scammells, P.J., 2012, Synthesis and pharmacological evaluation of dual acting antioxidant A2A adenosine receptor agonists, Journal of Medicinal Chemistry [P], vol 55, issue 7, American Chemical Society, USA, pp. 3521-3534.

Goulburn, A., Alden, D., Davis, R.P., Micallef, S.J., Ng, E.S., Yu, Q.C., Lim, S.M., Soh, C., Elliott, D., Hatzistavrou, T., Bourke, J., Watmuff, B., Lang, R.J., Haynes, J.M., Pouton, C.W., Giudice, A., Trounson, A.O., Anderson, S.A., Stanley, E., Elefanty, A., 2011, A targeted NKX2.1 hesc reporter line enables identification of human basal forebrain derivatives, Stem Cells [P], vol 29, issue 3, AlphaMed Press, Inc., USA, pp. 462-473.

White, C., Short, J., Haynes, J., Matsui, M., Ventura, S., 2011, Contractions of the Mouse Prostate Elicited by Acetylcholine Are Mediated by M(3) Muscarinic Receptors, Journal Of Pharmacology And Experimental Therapeutics [P], vol 339, issue 3, AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS, USA, pp. 870-877.

Nefzger, C., Haynes, J., Pouton, C., 2011, Directed expression of gata2, mash1, and foxa2 synergize to induce the serotonergic neuron phenotype during in vitro differentiation of embryonic stem cells, Stem Cells [P], vol 29, issue 6, WILEY-BLACKWELL, USA, pp. 928-939.

Zeng, W., Fabb, S., Haynes, J., Pouton, C., 2011, Extended periods of neural induction and propagation of embryonic stem cell-derived neural progenitors with EGF and FGF2 enhances Lmx1 a expression and neurogenic potential, Neurochemistry International [P], vol 59, issue 3, PERGAMON-ELSEVIER SCIENCE LTD, ENGLAND, pp. 394-403.

Khaira, S., Nefzger, C., Beh, S., Pouton, C., Haynes, J., 2011, Midbrain and forebrain patterning delivers immunocytochemically and functionally similar populations of neuropeptide Y containing GABAergic neurons, Neurochemistry International [P], vol 59, issue 3, PERGAMON-ELSEVIER SCIENCE LTD, ENGLAND, pp. 413-420.

Elliott, D.A., Braam, S.R., Koutsis, K., S Ng, E., Jenny, R., Lagerqvist, E.L., Biben, C., Hatzistavrou, T., Hirst, C.E., Yu, Q.C., Skelton, R.J., Ward-van Oostwaard, D., Lim, S.M., Khammy, O., Li, X., Hawes, S.M., Davis, R.P., Goulburn, A.L., Passier, R., Prall, O.W., Haynes, J.M., Pouton, C.W., Kaye, D.M., Mummery, C.L., Elefanty, A.G., Stanley, E., 2011, NKX2-5eGFP/w hESCs for isolation of human cardiac progenitors and cardiomyocytes, Nature Methods [P], vol 8, issue 12, Nature Publishing Group, UK, pp. 1037-1040.

Ventura, S., Oliver, V., White, C., Xie, A., Haynes, J., Exintaris, B., 2011, Novel drug targets for the pharmacotherapy of benign prostatic hyperplasia (BPH), British Journal of Pharmacology [P], vol 163, issue 5, John Wiley & Sons Ltd., United Kingdom, pp. 891-907.

Urmaliya, V., Pouton, C., Devine, S., Haynes, J., Warfe, L., Scammells, P., White, P., 2010, A novel highly selective adenosine A1 receptor agonist VCP28 reduces ischemia injury in a cardiac cell line and ischemia-reperfusion injury in isolated rat hearts at concentrations that do not affect heart rate, Journal Of Cardiovascular Pharmacology [P], vol 56, issue 3, Lippincott Williams & Wilkins, United States, pp. 282-292.

Oliver, V.L., Anderson, C.J., Ventura, S., Haynes, J.M., 2010, Androgens regulate adenylate cyclase activity and intracellular calciumin stromal cells derived from human prostate, Prostate [P], vol 70, John Wiley & Sons, Inc., USA, pp. 1222-1232.

Chen, Q., Jin, L., Cook, W., Mohn, D., Lagerqvist, E., Elliott, D., Haynes, J., Boyd, N., Stark, W., Pouton, C., Stanley, E., Elefanty, A., 2010, Elastomeric nanocomposites as cell delivery vehicles and cardiac support devices, Soft Matter [P], vol 6, issue 19, Royal Society of Chemistry, UK, pp. 4715-4726.

Lagerqvist, E.L., Finnin, B.A.L., Pouton, C.W., Haynes, J.M., 2010, Endothelin-1 and angiotensin II modulate rate and contraction amplitude in a subpopulation of mouse embryonic stem cell-derived cardiomyocyte-containing bodies, Stem Cell Research [P], vol 6, issue 1, Elsevier BV, Netherlands, pp. 23-33.

White, C., Short, J.L., Haynes, J.M., Evans, R.J., Ventura, S., 2010, The residual nonadrenergic contractile response to nerve stimulation of the mouse prostate is mediated by acetylcholine but not ATP in a comparison with the mouse vas deferens, Journal Of Pharmacology And Experimental Therapeutics [P], vol 335, issue 2, The American Society for Pharmacology and Experimental Therapeutics, USA, pp. 489-496.

Xie, J.H., Hollande, F., Haynes, J.M., Pouton, C.W., Ventura, S., 2010, Troubleshooting immunohistochemical labelling of proliferating cell nuclear antigen (PCNA) in cryocut tissue sections of mouse prostate, Journal of Pharmacological and Toxicological Methods [P], vol 61, Elsevier Inc, United States, pp. 98-101.

Khaira, S.K., Pouton, C.W., Haynes, J.M., 2009, P2X2, P2X4 and P2Y1 receptors elevate intracellular Ca2+ in mouse embryonic stem cell-derived GABAergic neurons, British Journal of Pharmacology [P], vol 158, issue 8, John Wiley and Sons Ltd, UK, pp. 1922-1931.

Haynes, J.M., 2008, Systemic stem cell improvement in cardiac function after ischaemic injury: leaping forward slowly, Clinical and Experimental Pharmacology and Physiology, vol 35, issue 2, Wiley-Blackwell Publishing Asia, Australia, pp. 105-106.

Pouton, C.W., Haynes, J.M., 2007, Embryonic stem cells as a source of models for drug discovery, Nature Reviews Drug Discovery, vol 6, issue 8, Nature Publishing Group, London, England, pp. 605-616.

Raye, W.S., Tochon-Danguy, N., Pouton, C.W., Haynes, J.M., 2007, Heterogeneous population of dopaminergic neurons derived from mouse embryonic stem cells: preliminary phenotyping based on receptor expression and function, European Journal of Neuroscience, vol 25, issue 7, Blackwell Publishing Ltd, UK, pp. 1961-1970.

Cook, A.M., Haynes, J.M., 2007, Phosphorylation of the PKG substrate, vasodilator-stimulated phosphoprotein (VASP), in human cultured prostatic stromal cells, Nitric Oxide-Biology and Chemistry, vol 16, issue 1, Academic Press Inc. Elsevier Science, San Diego USA, pp. 10-17.

Nguyen, S.T., Prakash, R., Anderson, C.J., Frydenberg, M., Haynes, J.M., 2007, Sex steroids modulate alpha1-adrenoceptor-stimulated Ca2+ elevation in human cultured prostatic stromal cells, The Prostate, vol 67, issue 1, John Wiley & Sons, Inc., Hoboken USA, pp. 74-82.

Haynes, J.M., 2007, beta2 and beta3-adrenoceptor inhibition of alpha1-adrenoceptor-stimulated Ca2+ elevation in human cultured prostatic stromal cells, European Journal of Pharmacology, vol 570, issue 1-3, Elsevier BV, Netherlands, pp. 18-26.

Haynes, J.M., Cook, A.M., 2006, Protein kinase G-induced activation of KATP channels reduces contractility of human prostate tissue, The Prostate, vol 66, issue 4, John Wiley & Sons, Inc., Hoboken USA, pp. 377-385.

Cao, N., Haynes, J.M., Ventura, S., 2006, Saw palmetto is an indirectly acting sympathomimetic in the rat-isolated prostate gland, The Prostate, vol 66, issue 2, John Wiley & Sons, Inc., Hoboken USA, pp. 115-123.

Haynes, J.M., Ventura, S., 2005, Current models of human prostate contractility, Clinical and Experimental Pharmacology and Physiology, vol 32, issue 10, Blackwell Publishing Asia, Australia, pp. 797-804.

Pouton, C.W., Haynes, J.M., 2005, Pharmaceutical applications of embryonic stem cells, Advanced Drug Delivery Reviews, vol 57, issue 13, Elsevier BV, Netherlands, pp. 1918-1934.

Preston, A., Frydenberg, M., Haynes, J.M., 2004, A1 and A2A adenosine receptor modulation of 1-adrenoceptor-mediated contractility in human cultured prostatic stromal cells, British Journal of Pharmacology, vol 141, issue 2, Nature Publishing Group, UK, pp. 302-310.

Lang, R.J., Haynes, J.M., Kelly, J., Johnson, J., Geenhalgh, J., O'Brien, C., Mulholland, E.L., Baker, L., Munsie, M., Pouton, C.W., 2004, Electrical and neurotransmitter activity of mature neurons derived from mouse embryonic stem cells by Sox-1 lineage selection and directed differentiation, European Journal of Neuroscience, vol 20, issue 12, Blackwell Publishing Ltd, UK, pp. 3209-3221.

Cook, A.M., Haynes, J.M., 2004, Protein kinase G II-mediated proliferative effects in human cultured prostatic stromal cells, Cellular Signalling, vol 16, issue 2, Elsevier Inc., USA, pp. 253-261.

Preston, A., Haynes, J.M., 2003, 1- Adrenoceptor effects mediated by protein kinase C in human cultured prostatic stromal cells, British Journal of Pharmacology, vol 138, Nature Publishing Group, UK, pp. 218-224.

Haynes, J.M., Pennefather, J.N., Sikorski, B., 2003, Purinoceptor-mediated contractility of the perfused uterine vasculature of the guinea-pig: influence of oestradiol and pregnancy, Clinical and Experimental Pharmacology and Physiology, vol 30, issue 5-Jun, Blackwell Publishing Asia, Australia, pp. 329-335.

Haynes, J.M., Iannazzo, L., Majewski, H., 2002, Phorbol ester-induced contractility and Ca2+ influx in human cultured prostatic stromal cells., Biochemical Pharmacology [P], vol 64, issue 3, Elsevier Inc., USA, pp. 385-392.

Cook, A., Frydenberg, M., Haynes, J.M., 2002, Protein kinase G activation of KATP channels in human-cultured prostatic stromal cells, Cellular Signalling, vol 14, issue 12, Elsevier Science Inc., USA, pp. 1023-1029.

Haynes, J.M., Wainer, J., Hodgson, W.H., Strasser, R., Cooper, M.E., 1997, Rat amylin mediates a pressor response in the anaesthetised rat: implications for the association between hypertension and diabetes mellitus, Diabetologia, vol 40, Springer-Verlag, Germany, pp. 256-261.

Conference Proceedings

Khaira, S.K., Pouton, C.W., Haynes, J.M., 2006, Basic pharmacological investigation of embryonic mouse stem cell derived GABAergic neurons, Proceedings of the 3rd Australian Health and Medical Research Congress, 26 Nov - 1 Dec 2006, Australian Health and Medical Research Congress, Melbourne Vic Australia, p. 427.

Khaira, S.K., Tochon-Danguy, N., Pouton, C.W., Haynes, J.M., 2006, Characterisation of GABAergic neurons derived from mouse embryonic stem cells, Cytometry Part A, John Wiley & Sons, Inc, USA, p. 419.

Khaira, S.K., Tochon-Danguy, N., Pouton, C.W., Haynes, J.M., 2006, Characterisation of GABAergic neurons drived from mouse embryonic stem cells, Proceedings of the 4th ISSCR Meeting, 29 June - 1 July 2006, p. 129.

Ventura, S., Cao, N., Haynes, J.M., 2006, Extracts of saw palmetto have indirectly acting sympatomimetic effects in the rat prostrate gland, XVth World Congress of Pharmacology: Meeting Abstracts, 2-7 July 2006, Blackwell Publishing, Beijing China, p. 336.

Nefzger, C., Haynes, J.M., Pouton, C.W., 2006, GABA and dopamine release from differentiated neurons derived from mouse ES cells in adherent monoculture or obtained via the formation of embryoid bodies, Proceedings of the 4th ISSCR meeting, 29 June - 1 July 2006, p. 211.

Haynes, J.M., Khaira, S.K., Tochon-Danguy, N., Pouton, C.W., 2006, GABAergic neurons derived from mouse embryonic stem cells: a brief characterization, Acta Pharmacologica Sinica, 2-7 July 2006, Blackwell Publishing, Beijing China, p. 238.

Haynes, J.M., Pennefather, J.N., Sikorski, B., 1998, Effects of purine nucleotides on the uterine vasculature of the guinea-pig, Pharmacology and Toxicology, Munchen, Germany, Aug.1998, Munksgaard, Copenhagen, p. 101 (P39).

Haynes, J.M., Wainer, J., Hodgson, W.H., Strasser, R., Cooper, M.E., 1997, Health Service Delivery in Small Rural Communities: The Process of Change, 4th National Rural Conference, Western Australia 9-12 February 1997, Promoco Conventions P/L, WA Australia, pp. 255-264.

Teaching Commitment

Lecture in:

• Bachelor of Pharmaceutical Sciences: renal physiology, pharmacology and second messenger signalling, Neurodegeneration (Parkinson’s disease)
• Bachelor of Pharmacy: renal physiology and diuretic action

Activities

Affiliations with institutes

Medicinal Chemistry and Drug Action
Monash Institute of Pharmaceutical Sciences

Postgraduate Research Supervisions

Current Supervision

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Effect of stem cell based therapy in murine models of Alzheimer's disease..

Supervisors:

Short, J (Main), Haynes, J (Associate), Nicolazzo, J (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Identification and isolation of prostate cancer stem cells from prostate cancer cell lines..

Supervisors:

Ventura, S (Main), Haynes, J (Associate), Hollande, F (Associate), Pouton, C (Associate), Sloan, E (Associate).

Completed Supervision

Student:

Cook, A.

Program of Study:

PROSTATIC STROMAL CELL FUNCTION: MODULATION OF CONTRACTILITY AND VIABILITY BY CGMP-DEPENDENT PROTEIN KINASE.. (PHD) 2004.

Supervisors:

Haynes, J (Main), Pouton, C (Associate).

Student:

Khaira, S.

Program of Study:

Characterisation of GABAergic neurons derived from mouse embryonic stem cells. (PHD) 2009.

Supervisors:

Haynes, J (Main), Hollande, F (Associate), Pouton, C (Associate).

Student:

Lagerqvist, E.

Program of Study:

Functional characterisation of cardiomyocytes derived from mouse and human embryonic stem cells. (PHD) 2011.

Supervisors:

Haynes, J (Joint-Co), Pouton, C (Joint).

Student:

Nefzger, C.

Program of Study:

Neural differentiation of murine embryonic stem cells with a focus on serotonergic neuron differentiation. (PHD) 2009.

Supervisors:

Haynes, J (Joint), Pouton, C (Joint-Co).

Student:

Oliver, V.

Program of Study:

Regulation of intracellular calcium by androgens in stromal cells cultured from the human prostate. (PHD) 2011.

Supervisors:

Haynes, J (Main).

Student:

Su, C.

Program of Study:

Investigating the development of midbrain dopaminergic neurons using mouse embryonic stem cell reporter lines. (PHD) 2012.

Supervisors:

Haynes, J (Main), Khaira, S (Associate), Nefzger, C (Associate).