Dr Martin Scanlon - Researcher Profile

Biography

Position

Group Leader
Structure-based drug design

Medicinal Chemistry and Drug Action
Monash Institute of Pharmaceutical Sciences

Research & Supervision Interests

Fragment-based drug design
Structure-based design of novel anti-microbial agents
Analysis of protein-ligand interactions
Protein structure determination and structural biology

Qualifications

DOCTOR OF PHILOSOPHY

Institution: University of Nottingham

Year awarded: 1993

CHEMISTRY

Institution: The University of Leeds

Year awarded: 1987

Publications

Journal Articles

White, P.J., Hickey, P.L., Tu, L.S., Headey, S.J., Scanlon, M., Boyd, B.J., Pouton, C.W., 2012, Colloidal characteristics and formulation of pure protein particulate vaccines, Journal of Pharmacy and Pharmacology [P], vol 64, issue 10, John Wiley & Sons Ltd, United Kingdom, pp. 1386-1393.

Walden, P.M., Heras, B., Chen, K., Halili, M.A., Rimmer, K., Sharma, P., Scanlon, M., Martin, J.L., 2012, The 1.2 A resolution crystal structure of TcpG, the Vibrio cholerae DsbA disulfide-forming protein required for pilus and cholera-toxin production, Acta Crystallographica Section D-Biological Crystallography [P], vol 68, issue 10, Wiley-Blackwell Publishing, Inc., USA, pp. 1290-1302.

Velkov, T., Horne, J., Scanlon, M., Capuano, B., Yuriev, E., Lawen, A., 2011, Characterization of the N-methyltransferase activities of the multifunctional polypeptide cyclosporin synthetase, Chemistry and Biology [P], vol 18, issue 4, Cell Press, USA, pp. 464-475.

Trevaskis, N., Nguyen, G., Scanlon, M., Porter, C., 2011, Fatty acid binding proteins: potential chaperones of cytosolic drug transport in the enterocyte?, Pharmaceutical Research [P], vol 28, issue 9, Kluwer Academic/Plenum Publishers, USA, pp. 2176-2190.

Wielens, J., Headey, S., Deadman, J., Rhodes, D., Le, G., Parker, M., Chalmers, D., Scanlon, M., 2011, Fragment-based design of ligands targeting a novel site on the integrase enzyme of human immunodeficiency virus 1, Chemmedchem [P], vol 6, issue 4, WILEY-BLACKWELL, USA, pp. 258-261.

Laguerre, A., Wielens, J., Parker, M., Porter, C., Scanlon, M., 2011, Preparation, crystallization and preliminary X-ray diffraction analysis of two intestinal fatty-acid binding proteins in the presence of 11-(dansylamino)undecanoic acid, Acta Crystallographica Section F: Structural Biology and Crystallization Communications [P], vol 67, issue 2, International Union of Crystallography, United Kingdom, pp. 291-295.

Doak, B., Scanlon, M., Simpson, J., 2011, Synthesis of unsymmetrical 1,1 '-disubstituted Bis(1,2,3-triazole)s using monosilylbutadiynes, Organic Letters [P], vol 13, issue 3, AMER CHEMICAL SOC, USA, pp. 537-539.

Chalmers, D., Martin, J., Scanlon, M., 2010, Backbone and side chain 1H, 15N and 13C assignments for the oxidised and reduced forms of the oxidoreductase protein DsbA from Staphylococcus aureus, Biomolecular NMR Assignments [P], vol 4, issue 1, Springer Netherlands, Netherlands, pp. 25-28.

Shouldice, S., Heras, B., Jarrott, R., Sharma, P., Scanlon, M., Martin, J., 2010, Characterization of the DsbA oxidative folding catalyst from Pseudomonas aeruginosa reveals a highly oxidizing protein that binds small molecules, Antioxidants and Redox Signaling [P], vol 12, issue 8, Mary Ann Liebert, Inc. Publishers, United States, pp. 921-931.

Wielens, J., Headey, S., Jeevarajah, D., Rhodes, D., Deadman, J., Chalmers, D., Scanlon, M., Parker, M., 2010, Crystal structure of the HIV-1 integrase core domain in complex with sucrose reveals details of an allosteric inhibitory binding site, FEBS Letters [P], vol 584, issue 8, Elsevier BV, Netherlands, pp. 1455-1462.

Robertson, A.L., Headey, S.J., Saunders, H.M., Ecroyd, H., Scanlon, M., Carver, J.A., Bottomley, S.P., 2010, Small heat-shock proteins interact with a flanking domain to suppress polyglutamine aggregation, Proceedings Of The National Academy Of Sciences Of The United States Of America [P], vol 107, issue 23, National Academy of Sciences, USA, pp. 10424-10429.

Velkov, T., Hughes, M.L.R., Horne, H.J., Simpson, J.S., Porter, C.J., Scanlon, M., 2009, Characterization of lipophilic drug binding to rat intestinal fatty acid binding protein, Molecular And Cellular Biochemistry [P], vol 326, issue 1-2, Springer New York LLC, USA, pp. 87-95.

Heras, B., Shouldice, S.R., Totsika, M., Scanlon, M., Schembri, M.A., Martin, J.L., 2009, DSB proteins and bacterial pathogenicity, Nature Reviews Microbiology [P], vol 7, issue 13, Nature Publishing Group, UK, pp. 215-225.

Roberts, B.P., Scanlon, M., Krippner, G.Y., Chalmers, D.K., 2009, Molecular Dynamics of Poly(L-lysine) Dendrimers with Naphthalene Disulfonate Caps, Macromolecules [P], vol 42, issue 7, American Chemical Society, USA, pp. 2775-2783.

Roberts, B.P., Krippner, G.Y., Scanlon, M., Chalmers, D.K., 2009, Molecular dynamics of variegated polyamide dendrimers, Macromolecules [P], vol 42, issue 7, American Chemical Society, USA, pp. 2784-2794.

Chuang, S., Velkov, T., Horne, H.J., Wielens, J., Chalmers, D.K., Porter, C.J., Scanlon, M., 2009, Probing the fibrate binding specificity of rat liver fatty acid binding protein, Journal of Medicinal Chemistry [P], vol 52, issue 17, American Chemical Society, USA, pp. 5344-5355.

Vivian, J., Scoullar, J., Rimmer, K., Bushell, S.R., Beddoe, T.C., Wilce, M.C.J., Byres, E., Boyle, T.P., Doak, B.C., Simpson, J.S., Graham, B., Heras, B., Kahler, C.M., Rossjohn, J., Scanlon, M., 2009, Structure and function of the oxidoreductase DsbA1 from Neisseria meningitidis, Journal of Molecular Biology [P], vol 394, issue 5, Academic Press, UK, pp. 931-943.

Paxman, J.J., Borg, N., Horne, H.J., Thompson, P., Chin, K.Y., Sharma, P., Simpson, J.S., Wielens, J., Piek, S., Kahler, C.M., Sakellaris, H., Pearce, M.C., Bottomley, S.P., Rossjohn, J., Scanlon, M., 2009, The Structure of the Bacterial Oxidoreductase Enzyme DsbA in Complex with a Peptide Reveals a Basis for Substrate Specificity in the Catalytic Cycle of DsbA Enzymes, Journal Of Biological Chemistry [P], vol 284, issue 26, American Society for Biochemistry and Molecular Biology, Inc., USA, pp. 17835-17845.

Headey, S.J., Vom, A., Simpson, J.S., Scanlon, M., 2008, Backbone assignments of the 34kDa ketopantoate reductase from E. coli, Biomolecular NMR Assignments [P], vol 2, issue 1, Springer Netherlands, Netherlands, pp. 93-96.

Chuang, S., Velkov, T., Horne, J., Porter, C.J., Scanlon, M., 2008, Characterization of the drug binding specificity of rat liver fatty acid binding protein, Journal of Medicinal Chemistry, vol 51, issue 13, American Chemical Society, USA, pp. 3755-3764.

Vivian, J., Scoullar, J., Robertson, A.L., Bottomley, S.P., Horne, H.J., Chin, K.Y., Wielens, J., Thompson, P., Velkov, T., Piek, S., Byres, E., Beddoe, T.C., Wilce, M.C., Kahler, C.M., Rossjohn, J., Scanlon, M., 2008, Structural and biochemical characterization of the oxidoreductase NmDsbA3 from Neisseria meningitidis, The Journal of Biological Chemistry, vol 283, issue 47, American Society for Biochemistry and Molecular Biology Inc., United States, pp. 32452-32461.

Roberts, B.P., Scanlon, M., Krippner, G.Y., Chalmers, D.K., 2008, The Dotted Cap Notation: A concise notation for describing variegated dendrimers, New Journal of Chemistry, vol 32, issue 9, Royal Society Chemistry, UK, pp. 1543-1554.

Robertson, A.L., Horne, H.J., Ellisdon, A.M., Thomas, B., Scanlon, M., Bottomley, S.P., 2008, The structural impact of a polyglutamine tract is location-dependent, Biophysical Journal, vol 95, issue 12, Biophysical Society, United States, pp. 5922-5930.

Horne, H.J., Scanlon, M., 2007, Backbone and side chain 1H, 15N and 13C assignments for the reduced form of the oxidoreductase protein DsbA from Vibrio cholerae, Biomolecular NMR Assignments, vol 1, Springer, Netherlands, pp. 75-76.

Velkov, T., Horne, H.J., Laguerre, A.K., Jones, E.D., Scanlon, M., Porter, C.J., 2007, Examination of the role of intestinal fatty acid-binding protein in drug absorption using a parallel artificial membrane permeability assay, Chemistry & Biology, vol 14, issue 4, Cell Press, USA, pp. 453-465.

Horne, H.J., d'Auvergne, E.J., Coles, M., Velkov, T., Chin, Y., Charman, W.N., Prankerd, R.J., Gooley, P., Scanlon, M., 2007, Probing the Flexibility of the DsbA Oxidoreductase from Vibrio cholerae-a 15N - 1H Heteronuclear NMR Relaxation Analysis of Oxidized and Reduced Forms of DsbA, Journal of Molecular Biology, vol 371, issue 3, Academic Press, England, pp. 703-716.

Perry, C.S., Charman, S.A., Prankerd, R.J., Chiu, F.C.K., Scanlon, M.J., Chalmers, D.K., Charman, W.N., 2006, The binding interaction of synthetic ozonide antimalarials with natural and modified beta-cyclodextrins, Journal of Pharmaceutical Sciences, vol 95, issue 1, John Wiley & Sons, Inc., USA, pp. 146-158.

Gibbs, G.M., Scanlon, M.J., Swarbrick, J.D., Curtis, S., Gallant, E., Dulhunty, A.F., O'Bryan, M.K., 2006, The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling, Journal of Biological Chemistry, vol 281, issue 7, American Society for Biochemistry and Molecular Biology, Inc., USA, pp. 4156-4163.

Velkov, T., Chuang, S., Prankerd, R.J., Sakellaris, H.H., Porter, C.J.H., Scanlon, M.J., 2005, An improved method for the purification of rat liver-type fatty acid binding protein from Escherichia coli, Protein Expression & Purification, vol 44, issue 1, Academic Press, USA, pp. 23-31.

Jennings, C.V., Rosengren, J., Daly, N.L., Plan, M., Stevens, J., Scanlon, M.J., Waine, C., Norman, D.G., Anderson, M.A., Craik, D.J., 2005, Isolation, solution structure, and insecticidal activity of kalata B2, a circular protein with a twist: Do Mobius strips exist in nature?, Biochemistry, vol 44, issue 3, American Chemical Society, Washington, USA, pp. 851-860.

Velkov, T., Chuang, S., Wielens, J., Sakellaris, H.H., Charman, W.N., Porter, C.J., Scanlon, M., 2005, The interaction of lipophilic drugs with intestinal fatty acid-binding protein, The Journal of Biological Chemistry, vol 280, issue 18, The American Society for Biochemistry and Molecular Biology, Inc., USA, pp. 17769-17776.

Chow, M.K., Mackay, J.P., Whisstock, J.C., Scanlon, M.J., Bottomley, S.P., 2004, Structural and functional analysis of the Josephin domain of the polyglutamine protein ataxin-3, Biochemical and Biophysical Research Communications, vol 322, issue 3, Academic Press Inc. Elsevier Science, San Diego USA, pp. 387-394.

Meutermans, W.D., Bourne, G.T., Golding, S.W., Horton, D.A., Campitelli, M.R., Craik, D.J., Scanlon, M., Smythe, M.L., 2003, Difficult macrocyclizations: New strategies for synthesizing highly strained cyclic tetrapeptides, Organic Letters [P], vol 5, issue 15, American Chemical Society, USA, pp. 2711-2714.

Shackleford, D.M., Prankerd, R.J., Scanlon, M.J., Charman, W.N., 2003, Self-micellization of gemfibrozil 1-0- acyl glucuronide in aqueous solution, Pharmaceutical Research, vol 20, issue 3, Kluwer Academic/Plenum Publishers, USA, pp. 465-470.

Lay, F.T., Schirra, H.J., Scanlon, M.J., Anderson, M.A., Craik, D.J., 2003, The three-dimensional solution structure of NaD1, a new floral defensin from Nicotiana alata and its application to a homology model of crop defense protein alfAFP, Journal of Molecular Biology, vol 325, Academic Press, UK, pp. 175-188.

Reid, R.C., Kelso, M.J., Scanlon, M.J., Fairlie, D.P., 2002, Conformationally constrained macrocycles that mimic tripeptide beta-strands in water and aprotic solvents, Journal of the American Chemical Society, vol 124, pp. 5673-5683.

Hayman, W.A., Toth, I., Flinn, N., Scanlon, M., Good, M.F., 2002, Enhancing the immunogenicity and modulating the fine epitope recognition of antisera to a helical group A streptococcal peptide vaccine candidate from the M protein using lipid-core peptide technology, Immunology And Cell Biology [P], vol 80, issue 2, Nature Publishing Group, UK, pp. 178-187.

Rosengren, K.J., Daly, N.I., Scanlon, M.J., Craik, D.J., 2001, Solution structure of BSTI: a new trypsin inhibitor from skin secretions of Bombina bombina, Biochemistry, vol 40, issue 15, American Chemical Society, USA, pp. 4601-4609.

Schirra, H.J., Scanlon, M.J., Lee, M.C.S., Anderson, M.A., Craik, D.J., 2001, The solution structure of C1T1, a two-domain proteinase inhibitor derived from a circular precursor protein from Nicotiana alata, Journal of Molecular Biology, vol 306, Academic Press, UK, pp. 69-79.

Fairlie, D.P., Tyndall, J.D., Reid, R.C., Wong, A.K., Abbenante, G., Scanlon, M., March, D.R., Bergman, D.A., Chai, C.L., Burkett, B.A., 2000, Conformational selection of inhibitors and substrates by proteolytic enzymes: Implications for drug design and polypeptide processing, Journal of Medicinal Chemistry, vol 43, American Chemical Society, USA, pp. 1271-1281.

Teaching Commitment

• Spectrometric identification of small molecules
• Drug-protein interactions

Activities

Affiliations with institutes

Medicinal Chemistry and Drug Action
Monash Institute of Pharmaceutical Sciences

Postgraduate Research Supervisions

Current Supervision

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

?-Conopeptide Analogues that Target Sodium Channels for the Treatment of Pain.

Supervisors:

Norton, R (Main), Scanlon, M (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Decreased brain levels of essential fatty acids in Alzheimer's disease: the role of the BBB.

Supervisors:

Nicolazzo, J (Main), Porter, C (Associate), Scanlon, M (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Do intracellular binding proteins facilitate drug absorption and drug targeting to the nucleus?.

Supervisors:

Porter, C (Joint), Scanlon, M (Joint-co).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Fatty acid binding protein at BBB: a novel taget for enhancing DHA in Alzeimer's disease?.

Supervisors:

Nicolazzo, J (Main), Porter, C (Associate), Scanlon, M (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Inhibitors of integrin-collagen binding as potential anti-cancer agents..

Supervisors:

Scanlon, M (Joint-co), Simpson, J (Joint).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Molecular insights into the interaction between plasmodium apical membrane antigen 1 and an invasion-inhibitory peptide.

Supervisors:

Scanlon, M (Joint-co), Simpson, J (Joint).

Completed Supervision

Student:

Chuang, S.

Program of Study:

The interactions of rat liver fatty acid binding protein with drug molecules. (PHD) 2008.

Supervisors:

Porter, C (Joint), Scanlon, M (Joint-Co).

Student:

Doak, B.

Program of Study:

Fragment based discovery of DsbA inhibitors. (PHD) 2012.

Supervisors:

Scanlon, M (Joint-Co), Simpson, J (Joint).

Student:

Laguerre, A.

Program of Study:

Characterising the drug binding sites of human intestinal and liver fatty acid binding proteins. (PHD) 2009.

Supervisors:

Porter, C (Joint-Co), Scanlon, M (Joint).

Student:

Paxman, J.

Program of Study:

The autotransporter SigA and the disulfide oxidoreductase DsbA from Shigella flexneri. (PHD) 2009.

Supervisors:

Crosby, I (Main), Sakelaris, H (Associate), Scanlon, M (Associate).

Student:

Roberts, B.

Program of Study:

Molecular dynamics simulations of dendrimers. (PHD) 2009.

Supervisors:

Chalmers, D (Main), Scanlon, M (Associate).

Student:

Sharma, P.

Program of Study:

Inhibition of oxidative protein folding in gram-negative bacteria: fragment-based design of DsbA inhibitors. (PHD) 2011.

Supervisors:

Scanlon, M (Main), Simpson, J (Associate), Manallack, D (Associate).

Student:

Williams, M.

Program of Study:

Fragment-based discovery and design of bacterial DsbA inhibitors. (PHD) 2012.

Supervisors:

Chalmers, D (Main), Scanlon, M (Associate).