Prof Trevor Lithgow - Researcher Profile

Biography

Evolutionary link provides clue to new disease control strategies

A series of ground-breaking findings about the nature of bacteria and their surface architecture have earned international recognition for Professor Trevor Lithgow. He has discovered ways to probe and analyze the molecular machines that bacteria use to build the outer surface. This knowledge lays the foundation for helping scientists to develop new ways to fight disease.

 

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Keywords

bacteria, bacterial evolution, membrane biochemistry, mitochondrial biology, mitochondrial evolution, protein transport

Qualifications

PHD

Institution: La Trobe University

Year awarded: 1992

BACHELOR OF SCIENCE WITH HONOURS

Institution: La Trobe University

Year awarded: 1985

Publications

Book Chapters

Likic, V.A., Dolezal, P., Celik, N., Dagley, M.J., Lithgow, T.J., 2010, Using hidden Markov models to discover new protein transport machines, in Protein Secretion - Methods and Protocols, eds Anastassios Economou, Humana Press, USA, pp. 271-284.

Journal Articles

Dunstan, R.A., Heinz, E., Wijeyewickrema, L.C., Pike, R.N., Purcell, A.W., Evans, T.J., Praszkier, J., Robins-Browne, R.M., Strugnell, R.A., Korotkov, K.V., Lithgow, T.J., 2013, Assembly of the type II secretion system such as found in Vibrio cholerae depends on the novel Pilotin AspS, PLoS ONE [P], vol 9, issue 1, Public Library of Science, USA, pp. 1-14.

Heinz, E., Lithgow, T., 2013, Back to basics: A revealing secondary reduction of the mitochondrial protein import pathway in diverse intracellular parasites, Biochimica et Biophysica Acta [P], vol 1833, issue 2, Elsevier BV, The Netherlands, pp. 295-303.

Shen, H., Lithgow, T.J., Martin, L.L., 2013, Reconstitution of membrane proteins into model membranes: seeking better ways to retain protein activities, International Journal of Molecular Sciences [P], vol 14, issue 1, M D P I AG, Switzerland, pp. 1589-1607.

Celik, N., Webb, C.T., Leyton, D.L., Holt, K.E., Heinz, E., Gorrell, R.J., Kwok-Scheulein, T., Naderer, T., Strugnell, R.A., Speed, T.P., Teasdale, R.D., Likic, V.A., Lithgow, T.J., 2012, A bioinformatic strategy for the detection, classification and analysis of bacterial autotransporters, PLoS ONE [P], vol 7, issue 8 (Art. ID: e43245), Public Library of Science, USA, pp. 1-15.

Hewitt, V.L., Heinz, E., Shingu-Vazquez, M.A., Qu, Y., Jelicic, B., Lo, T.L., Beilharz, T.H., Dumsday, G., Gabriel, K., Traven, A., Lithgow, T.J., 2012, A model system for mitochondrial biogenesis reveals evolutionary rewiring of protein import and membrane assembly pathways, Proceedings Of The National Academy Of Sciences Of The United States Of America [P], vol 109, issue 49, National Academy of Sciences, USA, pp. E3358-E3366.

Pusnik, M., Mani, J., Schmidt, O.I., Niemann, M., Oeljeklaus, S., Schnarwiler, F., Warscheid, B., Lithgow, T.J., Meisinger, C., Schneider, A., 2012, An essential novel component of the noncanonical mitochondrial outer membrane protein import system of trypanosomatids, Molecular Biology of the Cell [E], vol 23, issue 17, American Society for Cell Biology, USA, pp. 3420-3428.

Selkrig, J., Mosbahi, K., Webb, C.T., Belousoff, M.J., Perry, A.J., Wells, T.J., Morris, F.C., Leyton, D.L., Totsika, M., Phan, M., Celik, N., Kelly, M., Oates, C., Hartland, E.L., Robins-Browne, R.M., Ramarathinam, S.H., Purcell, A.W., Schembri, M.A., Strugnell, R.A., Henderson, I.R., Walker, D., Lithgow, T.J., 2012, Discovery of an archetypal protein transport system in bacterial outer membranes, Nature Structural and Molecular Biology [E], vol 19, issue 5 (S 1), Nature Publishing Group, USA, pp. 506-510.

Webb, C.T., Selkrig, J.P., Perry, A.J., Noinaj, N., Buchanan, S.K., Lithgow, T.J., 2012, Dynamic association of BAM complex modules includes surface exposure of the lipoprotein BamC, Journal of Molecular Biology [P], vol 422, issue 4, Academic Press, UK, pp. 545-555.

Webb, C.T., Heinz, E., Lithgow, T.J., 2012, Evolution of the beta-barrel assembly machinery, Trends in Microbiology [P], vol 20, issue 12, Elsevier Ltd * Trends Journals, UK, pp. 612-620.

Dolezal, P., Aili, M., Tong, J., Jiang, J., Marobbio, C.M., Lee, S., Schuelein, R., Belluzzo, S., Binova, E., Mousnier, A., Frankel, G.M., Giannuzzi, G., Palmieri, F., Gabriel, K., Naderer, T., Hartland, E.L., Lithgow, T.J., 2012, Legionella pneumophila secretes a mitochondrial carrier protein during infection, Plos Pathogens [P], vol 8, issue 1 (Art. ID: e1002459), Public Library of Science, USA, pp. 1-13.

Qu, Y., Jelicic, B., Pettolino, F.A., Perry, A.J., Lo, T., Hewitt, V.L., Bantun, F., Beilharz, T.H., Peleg, A.y., Lithgow, T.j., Djordjevic, J., Traven, A., 2012, Mitochondrial sorting and assembly machinery subunit Sam37 in Candida albicans: insight into the roles of mitochondria in fitness, cell wall integrity, and virulence, Eukaryotic Cell [P], vol 11, issue 4, American Society for Microbiology, USA, pp. 532-544.

Pusnik, M., Schmidt, O.I., Perry, A.J., Oeljeklaus, S., Niemann, M., Warscheid, B., Meisinger, C., Lithgow, T.J., Schneider, A., 2012, Response to Zarsky et al. [Letter to the Editor], Current Biology [P], vol 22, issue 12, Cell Press, USA, pp. R481-R482.

Anwari, K., Webb, C.T., Poggio, S., Perry, A.J., Belousoff, M.j., Celik, N., Ramm, G., Lovering, A., Sockett, R.E., Smit, J., Jacobs-Wagner, C., Lithgow, T.J., 2012, The evolution of new lipoprotein subunits of the bacterial outer membrane BAM complex, Molecular Microbiology [P], vol 84, issue 5, Wiley-Blackwell Publishing Ltd, UK, pp. 832-844.

Baldi, D.L., Higginson, E.E., Hocking, D.M., Praszkier, J., Cavaliere, R., James, C.E., Bennett-Wood, V., Azzopardi, K.I., Turnbull, L., Lithgow, T.J., Robins-Browne, R.M., Whitchurch, C.B., Tauschek, M., 2012, The type II secretion system and its ubiquitous lipoprotein substrate, SslE, are required for biofilm formation and virulence of enteropathogenic Escherichia coli, Infection And Immunity [P], vol 80, issue 6, American Society for Microbiology, USA, pp. 2042-2052.

Alcock, F., Webb, C., Dolezal, P., Hewitt, V.L., Shingu-Vazquez, M.A., Likic, V., Traven, A., Lithgow, T.J., 2011, A small Tim homohexamer in the relict mitochondrion of Cryptosporidium, Molecular Biology And Evolution [P], vol E, Oxford University Press, UK, pp. 113-122.

Tong, J.H.S., Dolezal, P., Selkrig, J., Crawford, S.A., Simpson, A.G., Noinaj, N., Buchanan, S.K., Gabriel, K., Lithgow, T.J., 2011, Ancestral and derived protein import pathways in the mitochondrion of Reclinomonas americana, Molecular Biology And Evolution [P], vol 28, issue 5, Oxford University Press, USA, pp. 1581-1591.

Dagley, M.J., Gentle, I.E., Beilharz, T.H., Pettolino, F.A., Djordjevic, J., Lo, T., Uwamahoro, N., Rupasinghe, T.W.T., Tull, D., McConville, M.J., Beaurepaire, C., Nantel, A., Lithgow, T.J., Mitchell, A.P., Traven, A., 2011, Cell wall integrity is linked to mitochondria and phospholipid homeostasis in Candida albicans through the activity of the post-transcriptional regulator Ccr4-Pop2, Molecular Microbiology [P], vol 79, issue 4, Wiley-Blackwell Publishing Ltd, UK, pp. 968-989.

Wilksch, J., Yang, J., Clements, A., Gabbe, J., Short, K., Cao, H., Cavaliere, R., James, C., Whitchurch, C., Schembri, M., Chuah, M., Liang, Z., Wijburg, O., Jenney, A., Lithgow, T., Strugnell, R., 2011, MrkH, a novel c-di-GMP-dependent transcriptional activator, controls Klebsiella pneumoniae biofilm formation by regulating type 3 fimbriae expression, Plos Pathogens [P], vol 7, issue 8 (Art. No: e1002204), Public Library of Science, USA, pp. 1-22.

Quenault, T.L., Lithgow, T.J., Traven, A., 2011, PUF proteins: repression, activation and mRNAlocalization, Trends in Cell Biology [P], vol 21, issue 2, Elsevier Ltd, UK, pp. 104-112.

Rimmer, K.A., Foo, J.H., Ng, A., Petrie, E.J., Shilling, P.J., Perry, A.J., Mertens, H.D., Lithgow, T.J., Mulhern, T.D., Gooley, P.R., 2011, Recognition of mitochondrial targeting sequences by the import receptors Tom20 and Tom22, Journal of Molecular Biology [P], vol 405, issue 3, Academic Press, UK, pp. 804-818.

Jiang, J., Davies, J.K., Lithgow, T.J., Strugnell, R.A., Gabriel, K., 2011, Targeting of Neisserial PorB to the mitochondrial outer membrane: An insight on the evolution of beta-barrel protein assembly machines, Molecular Microbiology [P], vol 82, issue 4, Wiley-Blackwell Publishing Ltd, UK, pp. 976-987.

Rada, P., Dolezal, P., Jedelsky, P.L., Bursac, D., Perry, A.J., Sedinova, M., Smiskova, K., Novotny, M., Campo Beltran, N., Hrdy, I., Lithgow, T.J., Tachezy, J., 2011, The core components of organelle biogenesis and membrane transport in the hydrogenosomes of Trichomonas vaginalis, PLoS ONE [P], vol 6, issue 9 (Art. No: e24428), Public Library of Science, USA, pp. 1-16.

Jedelsky, P.L., Dolezal, P., Rada, P., Pyrih, J., Smid, O., Hrdy, I., Sedinova, M., Marcincikova, M., Voleman, L., Perry, A.J., Campo Beltran, N., Lithgow, T.J., Tachezy, J., 2011, The minimal proteome in the reduced mitochondrion of the parasitic protist Giardia intestinalis, PLoS ONE [P], vol 6, issue 2 (Art. No: e17285), Public Library of Science, USA, pp. 1-17.

Becker, T., Wenz, L., Kruger, V., Lehmann, W., Muller, J., Goroncy, L., Zufall, N., Lithgow, T., Guiard, B., Chacinska, A., Wagner, R., Meisinger, C., Pfanner, N., 2011, The mitochondrial import protein Mim1 promotes biogenesis of multispanning outer membrane proteins, Journal of Cell Biology [P], vol 194, issue 3, Rockefeller University Press, USA, pp. 387-395.

Tsaousis, A.D., Gaston, D., Stechmann, A., Walker, P.B., Lithgow, T.J., Roger, A.J., 2010, A functional Tom70 in the human parasite Blastocystis sp.: Implications for the evolution of the mitochondrial import apparatus, Molecular Biology And Evolution [P], vol 28, issue 1, Oxford University Press, USA, pp. 781-791.

Anwari, K., Poggio, S., Perry, A., Gatsos, X., Ramarathinam, S.H., Williamson, N.A., Noinaj, N., Buchanan, S., Gabriel, K., Purcell, A.W., Jacobs-Wagner, C., Lithgow, T., 2010, A modular BAM complex in the outer membrane of the alpha-proteobacterium caulobacter crescentus, PLoS ONE [P], vol 5, issue 1 (e8619), Public Library of Science, USA, pp. 1-8.

Foo, J.H., Culvenor, J., Ferrero, R.L., Kwok-Schuelein, T., Lithgow, T.J., Gabriel, K., 2010, Both the p33 and p55 subunits of the Helicobacter pylori VacA toxin are targeted to mammalian mitochondria, Journal of Molecular Biology [P], vol 401, issue 5, Academic Press, UK, pp. 792-798.

Lithgow, T.J., Schneider, A., 2010, Evolution of macromolecular import pathways in mitochondria, hydrogenosomes and mitosomes, Philosophical Transactions of the Royal Society of London Series B Biological Sciences [P], vol 365, issue 1541, Royal Society, UK, pp. 799-817.

Alcock, F.H., Clements, A., Webb, C., Lithgow, T.J., 2010, Evolution: Tinkering inside the organelle, Science [P], vol 327, issue 5966, American Association for the Advancement of Science, USA, pp. 649-650.

Hewitt, V., Alcock, F., Lithgow, T., 2010, Minor modifications and major adaptations: The evolution of molecular machines driving mitochondrial protein import, Biochimica et Biophysica Acta - Biomembranes [P], vol 1808, issue 3, Elsevier BV, The Netherlands, pp. 947-954.

Webb, C., Lithgow, T.J., 2010, Mitochondrial biogenesis: Sorting mechanisms cooperate in ABC transporter assembly, Current Biology [P], vol 20, issue 13, Cell Press, USA, pp. R564-R567.

Likic, V.A., McConville, M.J., Lithgow, T.J., Bacic, A., 2010, Systems biology: the next frontier for bioinformatics, Advances in Bioinformatics [P], vol 2010, Hindawi Publishing Corporation, USA, pp. 1-10.

Dolezal, P., Dagley, M.J., Kono, M., Wolynec, P., Likic, V.A., Foo, J.H., Sedinova, M., Tachezy, J., Bachmann, A., Bruchhaus, I., Lithgow, T.J., 2010, The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica, Plos Pathogens [P], vol 6, issue 3 (e1000812), Public Library of Science, USA, pp. 1-13.

Traven, A., Lo, T.L., Lithgow, T.J., Heierhorst, J., 2010, The yeast PUF protein Puf5 has Pop2-independent roles in response to DNA replication stress, PLoS ONE [P], vol 5, issue 5 (e10651), Public Library of Science, USA, pp. 1-10.

Mills, R.D., Trewhella, J., Qiu, T.W., Welte, T., Ryan, T.M., Hanley, T., Knott, R.R., Lithgow, T.J., Mulhern, T.D., 2009, Domain organization of the monomeric form of the Tom70 mitochondrial import receptor, Journal of Molecular Biology [P], vol 388, issue 5, Academic Press, UK, pp. 1043-1058.

Waller, R.F., Jabbour, C., Chan, N.C., Celik, N., Likic, V.A., Mulhern, T.D., Lithgow, T.J., 2009, Evidence of a reduced and modified mitochondrial protein import apparatus in microsporidian mitosomes, Eukaryotic Cell [P], vol 8, issue 1, American Society for Microbiology, USA, pp. 19-26.

Chacinska, A., Koehler, C.M., Milenkovic, D., Lithgow, T.J., Pfanner, N., 2009, Importing mitochondrial proteins: Machineries and mechanisms, Cell [P], vol 138, issue 4, Cell Press, USA, pp. 628-644.

Bursac, D., Lithgow, T.J., 2009, Jid1 is a J-protein functioning in the mitochondrial matrix, unable to directly participate in endoplasmic reticulum associated protein degradation, FEBS Letters [P], vol 583, issue 17, Elsevier BV, The Netherlands, Denmark, pp. 2954-2958.

Dagley, M.J., Dolezal, P., Likic, V.A., Smid, O., Purcell, A.W., Buchanan, S.K., Tachezy, J., Lithgow, T.J., 2009, The protein import channel in the outer mitosomal membrane of Giardia intestinalis, Molecular Biology And Evolution [P], vol 26, issue 9, Oxford University Press, USA, pp. 1941-1947.

Clements, A., Bursac, D., Gatsos, X., Perry, A.J., Civciristov, S., Celik, N., Likic, V.A., Poggio, S., Jacobs-Wagner, C., Strugnell, R.A., Lithgow, T.J., 2009, The reducible complexity of a mitochondrial molecular machine, Proceedings Of The National Academy Of Sciences O..., vol 106, issue 37, National Academy of Sciences, USA, pp. 15791-15795.

Lueder, F.B., Lithgow, T.J., 2009, The three domains of the mitochondrial outer membrane protein Mim1 have discrete functions in assembly of the TOM complex, FEBS Letters [P], vol 583, issue 9, Elsevier BV, The Netherlands, Denmark, pp. 1475-1480.

Hartland, E.L., Lithgow, T.J., 2008, Bacterial protein secretion and trafficking down under, Future Microbiology [P], vol 3, issue 5, Future Medicine Ltd., UK, pp. 489-491.

Alcock, F.H., Grossmann, J.G., Gentle, I.E., Likic, V.A., Lithgow, T.J., Tokatlidis, K., 2008, Conserved substrate binding by chaperones in the bacterial periplasm and the mitochondrial intermembrane space, Biochemical Journal, vol 409, issue 2, Portland Press Ltd, UK, pp. 377-387.

Gatsos, X., Perry, A.J., Anwari, K., Dolezal, P., Wolynec, P.P., Likic, V.A., Purcell, A.W., Buchanan, S.K., Lithgow, T.J., 2008, Protein secretion and outer membrane assembly in Alphaproteobacteria, FEMS Microbiology Reviews, vol 32, issue 6, Wiley-Blackwell Publishing Ltd, UK, The Netherlands, pp. 995-1009.

Perry, A.J., Rimmer, K.A., Mertens, H.D., Waller, R., Mulhern, T.D., Lithgow, T.J., Gooley, P.R., 2008, Structure, topology and function of the translocase of the outer membrane of mitochondria, Plant Physiology and Biochemistry, vol 46, issue 3, Elsevier Masson, France, pp. 265-274.

Schneider, A., Bursac, D., Lithgow, T.J., 2008, The direct route: a simplified pathway for protein import into the mitochondrion of trypanosomes, Trends in Cell Biology, vol 18, issue 1, Elsevier Ltd, UK, pp. 12-18.

Chan, N.C., Lithgow, T.J., 2008, The peripheral membrane subunits of the SAM complex function codependently in mitochondrial outer membrane biogenesis, Molecular Biology of the Cell, vol 19, issue 1, American Society for Cell Biology, USA, pp. 126-136.

Hulett, J.M., Lueder, F.B., Chan, N.C., Perry, A.J., Wolynec, P., Likic, V.A., Gooley, P.R., Lithgow, T.J., 2008, The transmembrane segment of Tom20 is recognized by Mim1 for docking to the mitochondrial TOM complex, Journal of Molecular Biology, vol 376, issue 3, Academic Press, UK, pp. 694-704.

Tilley, L., Sougrat, R., Lithgow, T.J., Hanssen, E., 2008, The twists and turns of Maurer's cleft trafficking in P. falciparum-infected erythrocytes, Traffic, vol 9, issue 2, Wiley-Blackwell Munksgaard, Denmark, pp. 187-197.

Gentle, I.E., Perry, A.J., Alcock, F.H., Likic, V.A., Dolezal, P., Ng, E.T., Purcell, A.W., McConnville, M., Naderer, T., Chanez, A., Charriere, F., Aschinger, C., Schneider, A., Tokatlidis, K., Lithgow, T.J., 2007, Conserved motifs reveal details of ancestry and structure in the small TIM chaperones of the mitochondrial intermembrane space, Molecular Biology and Evolution, vol 24, issue 5, Oxford University Press, USA, pp. 1149-1160.

Hulett, J.M., Walsh, P., Lithgow, T.J., 2007, Domain stealing by receptors in a protein transport complex, Molecular Biology and Evolution, vol 24, issue 9, Oxford University Press, USA & Australia, pp. 1909-1911.

Adisa, A., Frankland, S., Rug, M., Jackson, K., Maier, A.G., Walsh, P., Lithgow, T.J., Klonis, N., Gilson, P.R., Cowman, A.F., Tilley, L., 2007, Re-assessing the locations of components of the classical vesicle-mediated trafficking machinery in transfected Plasmodium falciparum, International Journal for Parasitology, vol 37, issue 10, Elsevier Ltd, UK, Australia, pp. 1127-1141.

Uboldi, A.D., Lueder, F.B., Walsh, P., Spurck, T., McFadden, G.I., Curtis, J., Likic, V.A., Perugini, M.A., Barson, M., Lithgow, T.J., Handman, E., 2006, A mitochondrial protein affects cell morphology, mitochondrial segregation and virulence in Leishmania, International Journal for Parasitology, vol 36, issue 14, Oxford University Press, USA, pp. 1499-1514.

Perry, A.J., Hulett, J.M., Likic, V.A., Lithgow, T.J., Gooley, P.R., 2006, Convergent evolution of receptors for protein import into mitochondria, Current Biology, vol 16, issue 3, Cell Press, USA, pp. 221-229.

Dolezal, P., Likic, V.A., Tachezy, J., Lithgow, T.J., 2006, Evolution of the molecular machines for protein import into mitochondria, Science, vol 313, issue 5785, American Association for the Advancement of Science, USA, pp. 314-318.

Jabbour, A.M., Puryer, M.A., Yu, J.Y., Lithgow, T.J., Riffkin, C.D., Ashley, D.M., Vaux, D.L., Ekert, P.G., Hawkins, C.J., 2006, Human Bcl-2 cannot directly inhibit the Caenorhabditis elegans Apaf-1 homologue CED-4, but can interact with EGL-1, Journal of Cell Science, vol 119, issue Pt. 12, The Company of Biologists Ltd, UK, pp. 2572-2582.

Burri, L., Vascotto, K., Gentle, I.E., Chan, N.C., Beilharz, T., Stapleton, D.I., Ramage, L., Lithgow, T.J., 2006, Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae, The FEBS Journal, vol 273, issue 7, Wiley-Blackwell Publishing Ltd, UK , Denmark, pp. 1507-1515.

Burri, L., Williams, B.A.P., Bursac, D., Lithgow, T.J., Keeling, P.J., 2006, Microsporidian mitosomes retain elements of the general mitochondrial targeting system, Proceedings of the National Academy of Sciences of the United States of America, vol 103, issue 43, National Academy of Sciences, USA, pp. 15916-15920.

Chan, N.C., Likic, V.A., Waller, R.F., Mulhern, T.D., Lithgow, T.J., 2006, The C-terminal TPR domain of Tom70 defines a family of mitochondrial protein import receptors found only in animals and fungi, Journal of Molecular Biology, vol 358, issue 4, Academic Press, UK, pp. 1010-1022.

Perry, A.J., Hulett, J.M., Lithgow, T.J., Gooley, P.R., 2005, 1H, 13C and 15N resonance assignments of the cytosolic domain of Tom20 from Arabidopsis thaliana, Journal of Biomolecular NMR, vol 33, issue 3, Springer Netherlands, The Netherlands, p. 198.

Dolezal, P., Smid, O., Rada, P., Zubacova, Z., Bursac, D., Sutak, R., Nebesarova, J., Lithgow, T.J., Tachezy, J., 2005, Giardia mitosomes and trichomonad hydrogenosomes share a common mode of protein targeting, Proceedings of the National Academy of Sciences of the United States of America, vol 102, issue 31, National Academy of Sciences, USA, pp. 10924-10929.

Burri, L., Strahm, Y., Hawkins, C.J., Gentle, I., Puryer, M.A., Verhagen, A.M., Callus, B., Vaux, D.L., Lithgow, T.J., 2005, Mature DIABLO/Smac is produced by the IMP protease complex on the mitochondrial inner membrane, Molecular Biology of the Cell, vol 16, issue 6, American Society for Cell Biology, USA, pp. 2926-2933.

Uren, R.T., Dewson, G., Bonzon, C., Lithgow, T.J., Newmeyer, D.D., Kluck, R.M., 2005, Mitochondrial release of pro-apoptotic proteins: Electrostatic interactions can hold cytochrome c but not Smac/DIABLO to mitochondrial membranes, The Journal of Biological Chemistry, vol 280, issue 3, American Society for Biochemistry and Molecular Biology, Inc., USA, pp. 2266-2274.

Gentle, I.E., Burri, L., Lithgow, T.J., 2005, Molecular architecture and function of the Omp85 family of proteins, Molecular Microbiology, vol 58, issue 5, Wiley-Blackwell Publishing Ltd, UK, pp. 1216-1225.

Likic, V.A., Perry, A.E., Hulett, J.M., Derby, M.C., Traven, A., Waller, R.F., Keeling, P.J., Koehler, C.M., Curran, S.P., Gooley, P.R., Lithgow, T.J., 2005, Patterns that define the four domains conserved in known and novel isoforms of the protein import receptor Tom20, Journal of Molecular Biology, vol 347, issue 1, Academic Press, UK, pp. 81-93.

Lister, R., Hulett, J.M., Lithgow, T.J., Whelan, J., 2005, Protein import into mitochondria: Origins and functions today, Molecular Membrane Biology, vol 22, issue 1-2, Informa Healthcare, UK, pp. 87-100.

Perry, A.E., Lithgow, T.J., 2005, Protein targeting: Entropy, energetics and modular machines, Current Biology, vol 15, issue 11, Cell Press, USA, pp. R423-R425.

Beddoe, T.C., Bushell, S.R., Perugini, M.A., Lithgow, T., Mulhern, T.D., Bottomley, S.P., Rossjohn, J., 2004, A biophysical analysis of the tetratricopeptide repeat-rich mitochondrial import receptor, Tom70, reveals an elongated monomer that is inherently flexible, unstable, and unfolds via a multistate pathway, Journal of Biological Chemistry, vol 279, issue 45, The American Society for Biochemistry and Molecular Biology, Inc., Bethesda USA, pp. 46448-46454.

Burri, L., Lithgow, T.J., 2004, A complete set of SNAREs in yeast, Traffic, vol 5, issue 1, Wiley-Blackwell Munksgaard, Denmark, pp. 45-52.

Pfanner, N., Wiedemann, N., Meisinger, C., Lithgow, T.J., 2004, Assembling the mitochondrial outer membrane, Nature Structural and Molecular Biology, vol 11, issue 11, Nature Publishing Group, USA, pp. 1044-1048.

Lucattini, R., Likic, V.A., Lithgow, T.J., 2004, Bacterial proteins predisposed for targeting to mitochondria, Molecular Biology and Evolution, vol 21, issue 4, Oxford University Press, USA, pp. 652-658.

Youker, R.T., Walsh, P., Beilharz, T., Lithgow, T.J., Brodsky, J.L., 2004, Distinct roles for the Hsp40 and Hsp90 molecular chaperones during cystic fibrosis transmembrane conductance regulator degradation in yeast, Molecular Biology of the Cell, vol 15, issue 11, American Society for Cell Biology, USA, pp. 4787-4797.

Traven, A., Huang, D.C.S., Lithgow, T.J., 2004, Protein hijacking: Key proteins held captive against their will, Cancer Cell, vol 5, issue 2, Cell Press, USA, pp. 107-108.

Walsh, P., Bursac, D., Law, Y.C., Cyr, D., Lithgow, T.J., 2004, The J-protein family: modulating protein assembly, disassembly and translocation, EMBO Reports, vol 5, issue 6, Nature Publishing Group, UK, Germany, pp. 567-571.

Gentle, I., Gabriel, K., Beech, P.L., Waller, R., Lithgow, T.J., 2004, The Omp85 family of proteins is essential for outer membrane biogenesis in mitochondria and bacteria, The Journal of Cell Biology, vol 164, issue 1, Rockefeller University Press, USA, pp. 19-24.

Macasev, D., Whelan, J., Newbigin, E.J., Silva-Filho, M.C., Mulhern, T.D., Lithgow, T.J., 2004, Tom22', an 8 kDa trans-site receptor in plants and protozoans, is a conserved feature of the TOM complex that appeared early in the evolution of eukaryotes, Molecular Biology and Evolution, vol 21, issue 8, Oxford University Press, USA & Australia, pp. 1557-1564.

Burri, L., Vascotto, K., Fredersdorf, S., Tiedt, R., Hall, M.N., Lithgow, T.J., 2004, Zim17, a novel zinc-finger protein essential for protein import into mitochondria, The Journal of Biological Chemistry, vol 279, issue 48, American Society for Biochemistry and Molecular Biology, Inc., USA, pp. 50243-50249.

Burri, L., Varlamov, O., Doege, C.A., Hofmann, K., Beilharz, T., Rothman, J.E., Sollner, T.H., Lithgow, T.J., 2003, A SNARE required for retrograde transport to the endoplasmic reticulum, Proceedings of the National Academy of Sciences of the United States of America, vol 100, issue 17, National Academy of Sciences, USA, pp. 9873-9877.

Beilharz, T., Egan, B., Silver, P.A., Hofmann, K., Lithgow, T.J., 2003, Bipartite signals mediate subcellular targeting of tail-anchored membrane proteins in Saccharomyces cerevisiae, Journal of Biological Chemistry, vol 278, issue 10, American Society for Biochemistry and Molecular Biology, Inc., USA, pp. 8219-8223.

Taylor, N.L., Rudhe, C., Hulett, J.M., Lithgow, T.J., Glaser, E., Day, D.A., Millar, A.H., Whelan, J., 2003, Environmental stresses inhibit and stimulate different protein import pathways in plant mitochondria, FEBS Letters, vol 547, issue 1, Elsevier BV, Netherlands, Denmark, pp. 125-130.

Wilson-Annan, J., O'Reilly, L.A., Crawford, S.A., Hausmann, G., Beaumont, J.G., Parma, L.P., Chen, L., Lackmann, M., Lithgow, T.J., Hinds, M.G., Day, C.L., Adams, J.M., Huang, D.C.S., 2003, Proapoptotic BH3-only proteins trigger membrane integration of prosurvival Bcl-w and neutralize its activity, The Journal of Cell Biology, vol 162, issue 5, Rockefeller University Press, USA, pp. 877-887.

Gabriel, K., Egan, B., Lithgow, T.J., 2003, Tom40, the import channel of the mitochondrial outer membrane, plays an active role in sorting imported proteins, EMBO Journal, vol 22, issue 10, Nature Publishing Group, UK & Germany, pp. 2380-2386.

Allen, R., Egan, B., Gabriel, K., Beilharz, T., Lithgow, T.J., 2002, A conserved proline residue is present in the transmembrane-spanning domain of Tom7 and other tail-anchored protein subunits of the TOM translocase, FEBS Letters, vol 514, issue 2-3, Elsevier BV, The Netherlands & Denmark, pp. 347-350.

Beddoe, T.C., Lithgow, T.J., 2002, REVIEW: Delivery of nascent polypeptides to the mitochondrial surface, Biochimica et Biophysica Acta, vol 1592, issue 1, Elsevier BV, The Netherlands, pp. 35-39.

George, R., Walsh, P., Beddoe, T.C., Lithgow, T.J., 2002, The nascent polypeptide-associated complex (NAC) promotes interaction of ribosomes with the mitochondrial surface in vivo, FEBS Letters, vol 516, issue 1-3, Elsevier BV, The Netherlands & Denmark, pp. 213-216.

Gautschi, M., Lilie, H., Funfschilling, U., Mun, A., Ross, S., Lithgow, T.J., Rucknagel, P., Rospert, S., 2001, RAC, a stable ribosome-associated complex in yeast formed by the DnaK-DnaJ homologs Ssz1p and zuotin, Proceedings of the National Academy of Sciences of the United States of America, vol 98, issue 7, National Academy of Sciences, USA, pp. 3762-3767.

Wattenberg, B., Lithgow, T.J., 2001, Targeting of C-terminal (tail)-anchored proteins: understanding how cytoplasmic activities are anchored to intracellular membranes, Traffic, vol 2, issue 1, Wiley-Blackwell Munksgaard, Denmark, pp. 66-71.

Gabriel, K., Buchanan, S.K., Lithgow, T.J., 2001, The alpha and the beta: protein translocation across mitochondrial and plastid outer membranes, Trends in Biochemical Sciences, vol 26, issue 1, Elsevier Ltd, UK & Swizterland, pp. 36-40.

Chavez, S., Beilharz, T., Rondon, A.G., Erdjument-Bromage, H., Tempst, P., Svejstrup, J.Q., Lithgow, T.J., Aguilera, A., 2000, A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae, The EMBO Journal, vol 19, issue 21, Nature Publishing Group, UK & Germany, pp. 5824-5834.

Macasev, D., Newbigin, E.J., Whelan, J., Lithgow, T.J., 2000, How do plant mitochondria avoid importing chloroplast proteins? Components of the import apparatus Tom20 and Tom22 from Arabidopsis differ from their fungal counterparts, Plant Physiology, vol 123, issue 3, American Society of Plant Biologists, USA, pp. 811-816.

Beech, P.L., Nheu, T., Schultz, T., Herbert, S., Lithgow, T.J., Gilson, P.R., McFadden, G.I., 2000, Mitochondrial FtsZ in a chromophyte alga, Science, vol 287, issue 5456, American Association for the Advancement of Science, USA, pp. 1276-1279.

Lithgow, T.J., 2000, Targeting of proteins to mitochondria, FEBS Letters, vol 476, issue 1-2, Elsevier BV, The Netherlands & Denmark, pp. 22-26.

Gratzer, S., Beilharz, T., Beddoe, T.C., Henry, M.F., Lithgow, T.J., 2000, The mitochondrial protein targeting suppressor (mts1) mutation maps to the mRNA-binding domain of Npl3p and affects translation on cytoplasmic polysomes, Molecular Microbiology, vol 35, issue 6, Wiley-Blackwell Publishing Ltd, UK, pp. 1277-1285.

Postgraduate Research Supervisions

Current Supervision

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Analysis of the regulation of plasmids from Clostridium perfringens.

Supervisors:

Lithgow, T (Main), Belousoff, M (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Assembly and Regulation of bacterial autotransporters: a molecular and biophysical investigation.

Supervisors:

Martin, L (Main), Leyton, D (Associate), Lithgow, T (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Assembly of the secretin protein super-family..

Supervisors:

Lithgow, T (Main).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Evolution of post-transcriptional regulatory networks in yeast.

Supervisors:

Traven, A (Main), Lithgow, T (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Helicobacter pylori Vacuolating cytotoxin (VacA) - examining its role at mitochondria.

Supervisors:

Gabriel, K (Joint-co), Kwok-Schuelein, T (Joint), Lithgow, T (Associate).

Program of Study:

(MASTER'S BY RESEARCH).

Thesis Title:

Investigating the role of Helicobacter pylori in E-cadherin regulation and epithelial to mesenchymal transition events .

Supervisors:

Kwok-Schuelein, T (Main), Lithgow, T (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Protein Secretion systems in Gram-Negative Bacteria..

Supervisors:

Lithgow, T (Main).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

SidF targeting and regulation of pro-apoptotic host molecules after Legionella infection.

Supervisors:

Lithgow, T (Main), Naderer, T (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Structural and functional characterisation of the Helicobacter pylori type IV secretion system.

Supervisors:

Kwok-Schuelein, T (Main), Lithgow, T (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

The Mediator of transcription in Candida albicans: roles in cell wall biogenesis, morphogenesis and virulence..

Supervisors:

Traven, A (Main), Lithgow, T (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

The architecture of the SAM complex: a molecular machine that drives membrane assembly..

Supervisors:

Lithgow, T (Main), Gabriel, K (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

The role of Neisserial "blebs" on host cell biology during infection.

Supervisors:

Gabriel, K (Main), Lithgow, T (Associate), Naderer, T (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Understanding the toxic activity of Neisserial outer membrane vesicles on host cells.

Supervisors:

Gabriel, K (Joint-co), Lithgow, T (Joint).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Understanding the trafficing of pathogenic proteins to mitochondria and their effects on mitochondrial function.

Supervisors:

Gabriel, K (Joint-co), Lithgow, T (Joint).

Completed Supervision

Student:

Anwari, K.

Program of Study:

Omp85 family proteins and the BAM complex in Caulobacter crescentus. (PHD) 2011.

Supervisors:

Lithgow, T (Main).