Dr Lakshmi Wijeyewickrema - Researcher Profile

Biography

Lakshmi works in the Faculty of Medicine, Nursing & Health Sciences at Monash University as a Research Fellow.

Qualifications

DOCTOR OF PHILOSOPHY-BIOCHEMISTRY & MOLECULAR BIOL

Institution: Monash University

Year awarded: 2007

MASTERS OF SCIENCE - BIOCHEMISTRY & MOLECULAR BIOL

Institution: Monash University

Year awarded: 2003

GRAD DIP OF EDUCATION (SECONDARY)

Institution: Monash University

Year awarded: 2001

BACHELOR OF SCIENCE WITH HONOURS

Institution: University of Greenwich- London

Year awarded: 1995

Publications

Book Chapters

Beinrohr, L., Murray-Rust, T., Dyksterhuis, L., Zavodszky, P., Gal, P., Pike, R., Wijeyewickrema, L., 2011, Serpins and the complement system, in Methods in Enzymology, Volume 499: Biology of Serpins, eds James C Whisstock and Phillip I Bird, Academic Press, UK, pp. 55-75.

Yongqing, T., Potempa, J., Pike, R., Wijeyewickrema, L., 2011, The lysine-specific gingipain of porphyromonas gingivalis : Importance to pathogenicity and potential strategies for inhibition, in Cysteine Proteases of Pathogenic Organisms, eds Mark W Robinson & John P Dalton, Springer Science+Business Media, USA, pp. 15-29.

Rowley, M.J., O'Connor, K., Wijeyewickrema, L.C., 2004, Phage display for epitope determination: a paradigm for identifying receptor-ligand interactions, in Biotechnology Annual Review Volume 10, eds M Raafat El-Gewely, Elsevier, Amsterdam The Netherlands, pp. 151-188.

Journal Articles

Dunstan, R.A., Heinz, E., Wijeyewickrema, L.C., Pike, R.N., Purcell, A.W., Evans, T.J., Praszkier, J., Robins-Browne, R.M., Strugnell, R.A., Korotkov, K.V., Lithgow, T.J., 2013, Assembly of the type II secretion system such as found in Vibrio cholerae depends on the novel Pilotin AspS, PLoS ONE [P], vol 9, issue 1, Public Library of Science, USA, pp. 1-14.

Hsieh, Y.S., Taleski, D., Wilkinson, B., Wijeyewickrema, L.C., Adams, T.E., Pike, R.N., Payne, R.J., 2012, Effect of O-glycosylation and tyrosine sulfation of leech-derived peptides on binding and inhibitory activity against thrombin, Chemical Communications [P], vol 48, issue 10, Royal Society of Chemistry Publications, UK, pp. 1547-1549.

Duncan, R.C., Mohlin, F.C., Taleski, D., Coetzer, T.H., Huntington, J.A., Payne, R.J., Blom, A.M., Pike, R.N., Wijeyewickrema, L.C., 2012, Identification of a catalytic exosite for complement component c4 on the serine protease domain of c1s, Journal Of Immunology [P], vol 189, issue 5, American Association of Immunologists, USA, pp. 2365-2373.

Yongqing, T., Drentin, N., Duncan, R.C., Wijeyewickrema, L.C., Pike, R.N., 2012, Mannose-binding lectin serine proteases and associated proteins of the lectin pathway of complement: two genes, five proteins and many functions?, Biochimica et Biophysica Acta - Proteins and Proteomics [P], vol 1824, issue 1, Elsevier Science BV, The Netherlands, pp. 253-262.

Mika, A., Reynolds, S.L., Mohlin, F.C., Willis, C., Swe, P.M., Pickering, D.A., Halilovic, V., Wijeyewickrema, L.C., Pike, R.N., Blom, A.M., Kemp, D.J., Fischer, K., 2012, Novel scabies mite serpins inhibit the three pathways of the human complement system, PLoS ONE [P], vol 7, issue 7 (Art. No.: e40489), Public Library of Science, USA, pp. 1-15.

Ng, N., Pierce, M.J., Webb, G., Ratnikov, B., Wijeyewickrema, L.C., Duncan, R., Robertson, A.L., Bottomley, S.P., Boyd, S.E., Pike, R.N., 2011, Discovery of amino acid motifs for thrombin cleavage and validation using a model substrate, Biochemistry [P], vol 50, issue 48, American Chemical Society, USA, pp. 10499-10507.

Duncan, R.C., Bergstrom, F.C., Coetzer, T.H., Blom, A.M., Wijeyewickrema, L.C., Pike, R.N., 2011, Multiple domains of MASP-2, an initiating complement protease, are required for interaction with its substrate C4, Molecular Immunology [P], vol E, Pergamon, UK, pp. 1-8.

Wong, W., Wijeyewickrema, L.C., Kennan, R.M., Reeve, S.B., Steer, D.L., Reboul, C., Smith, A.I., Pike, R.N., Rood, J.I., Whisstock, J.C., Porter, C.J., 2011, S1 pocket of a bacterially derived subtilisin-like protease underpins effective tissue destruction, Journal Of Biological Chemistry [P], vol 286, issue 49, American Society for Biochemistry and Molecular Biology, Inc., USA, pp. 42180-42187.

Wijeyewickrema, L.C., Gardiner, E.E., Gladigau, E.L., Berndt, M.C., Andrews, R.K., 2010, Nerve growth factor inhibits metalloproteinase-disintegrins and blocks ectodomain shedding of platelet glycoprotein VI, Journal Of Biological Chemistry [P], vol 285, issue 16, American Society for Biochemistry and Molecular Biology, Inc., USA, pp. 11793-11799.

Beckham, S.A., Piedrafita, D.M., Phillips, C.I., Samarawickrema, N., Law, R.H.P., Smooker, P.M., Quinsey, N.S., Irving, J.A., Greenwood, D.L., Verhelst, S.H.L., Bogyo, M., Turk, B., Coetzer, T.H., Wijeyewickrema, L.C., Spithill, T.W., Pike, R.N., 2009, A major cathepsin B protease from the liver fluke Fasciola hepatica has atypical active site features and a potential role in the digestive tract of newly excysted juvenile parasites, International Journal of Biochemistry and Cell Biolog..., vol 41, issue 7, Pergamon, UK, pp. 1601-1612.

Beckham, S.A., Boyd, S.E., Reynolds, S., Willis, C., Johnstone, M., Mika, A., Simerska, P., Wijeyewickrema, L.C., Smith, A.I., Kemp, D.J., Pike, R.N., Fischer, K., 2009, Characterization of a serine protease homologous to house dust mite group 3 allergens from the scabies mite sarcoptes scabiei, Journal Of Biological Chemistry [P], vol 284, issue 49, American Society for Biochemistry & Molecular Biology Inc, USA, pp. 34413-34422.

Ng, N.M., Quinsey, N.S., Matthews, A.Y., Kaiserman, D., Wijeyewickrema, L.C., Bird, P.I., Thompson, P.E., Pike, R.N., 2009, The effects of exosite occupancy on the substrate specificity of thrombin, Archives Of Biochemistry And Biophysics [P], vol 489, issue 1-2, Academic Press, USA, pp. 48-54.

Fitzpatrick, R.E., Wijeyewickrema, L.C., Pike, R.N., 2009, The gingipains: scissors and glue of the periodontal pathogen, Porphyromonas gingivalis, Future Microbiology [P], vol 4, issue 4, Future Medicine Ltd, UK, pp. 471-487.

Kerr, F.K., Thomas, A.R., Wijeyewickrema, L.C., Whisstock, J., Boyd, S.E., Kaiserman, D., Matthews, A.Y., Bird, P.I., Thielens, N.M., Rossi, V., Pike, R.N., 2008, Elucidation of the substrate specificity of the MASP-2 protease of the lectin complement pathway and identification of the enzyme as a major physiological target of the serpin, C1-inhibitor, Molecular Immunology, vol 45, Pergamon-Elsevier Science Ltd, Oxford England UK, pp. 670-677.

Duncan, R.C., Wijeyewickrema, L.C., Pike, R.N., 2008, The initiating proteases of the complement system: controlling the cleavage, Biochimie, vol 90, Elsevier France-Editions Scientifiquers Medicales Elsevier, Paris France, pp. 387-395.

Wijeyewickrema, L.C., Gardiner, E.E., Shen, Y., Berndt, M.C., Andrews, R.K., 2007, Fractionation of snake venom metalloproteinases by metal ion affinity: A purified cobra metalloproteinase, Nk, from Naja kaouthia binds Ni2+-agarose, Toxicon, vol 50, issue 8, Pergamon-Elsevier Science Ltd, Oxford England UK, pp. 1064-1072.

Wijeyewickrema, L.C., Gardiner, E.E., Moroi, M., Berndt, M.C., Andrews, R.K., 2007, Snake venom metalloproteinases, crotarhagin and alborhagin, induce ectodomain shedding of the platelet collagen receptor, glycoprotein VI, Thrombosis & Haemostasis, vol 98, issue 6, Schattauer GMBH, Stuttgart Germany, pp. 1285-1290.

Arthur, J.F., Gardiner, E.E., Matzaris, M., Taylor, S.G., Wijeyewickrema, L.C., Ozaki, Y., Kahn, M.L., Andrews, R.K., Berndt, M.C., 2005, Glycoprotein VI is associated with GPIb-IX-V on the membrane of resting and activated platelets, Thrombosis and Haemostasis, vol 93, issue 4, Schattauer GmbH-Verlag Medizin Naturwissenschaften, Stuttgart Germany, pp. 716-723.

O'Connor, K.H., Konigs, C., Rowley, M.J., Irving, J.A., Wijeyewickrema, L.C., Pustowka, A., Dietrich, U., Mackay, I.R., 2005, Requirement of multiple phage displayed peptide libraries for optimal mapping of a conformational antibody epitope on CCR5, Journal of Immunological Methods, vol 299, issue 1-2, Elsevier, Amsterdam The Netherlands, pp. 21-35.

Wijeyewickrema, L.C., Berndt, M.C., Andrews, R.K., 2005, Snake venom probes of platelet adhesion receptors and their ligands, Toxicon, vol 45, issue 8, Pergamon-Elsevier Science Ltd, Kidlington England, pp. 1051-1061.

Rowley, M.J., Scealy, M., Whisstock, J.C., Jois, J.A., Wijeyewickrema, L.C., Mackay, I.R., 2000, Prediction of the immunodominant epitope of the pyruvate dehydrogenase complex E2 in primary biliary cirrhosis using phage display, Journal of Immunology, vol 164 issue 1, American Association of Immunologists, Bethesda USA, pp. 3413-3419.

Postgraduate Research Supervisions

Current Supervision

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

The kinetic mechanism of interaction between the complement porteases C1s and MASP-2 and the substrate C4?.

Supervisors:

Pike, R (Main), Wijeyewickrema, L (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

The structure and function of the human conplement protease, MASP-3.

Supervisors:

Pike, R (Main), Wijeyewickrema, L (Associate).