Dr Reinhard Boysen - Researcher Profile

Biography

Reinhard works in the Centre for Green Chemistry at Monash University as a Research Fellow.

 

Reinhard’s research areas of interest are:

·         Capillary based separation technologies

·         Mass spectrometry

·         Solid phase synthesis technologies encompassing ligand design

·         Biomedical diagnostics

 

 

 

 

 

 

Keywords

capillary based separation technologies, mass spectrometry, solid phase synthesis technologies

Qualifications

PHD IN NATURAL SCIENCES (DR. RER. NAT.)

Institution: Freie Universit?t Berlin, Germany

Year awarded: 1994

Publications

Book Chapters

Boysen, R.I., Hearn, M.T.W., 2012, High perfomance liquid chromatography of peptides and proteins, in Amino acids, peptides and proteins in organic chemistry, eds Andrew B.Hughes, Wiley-VCH, Weinheim Germany, pp. 167-210.

Boysen, R.I., Hearn, M.T.W., 2010, High performance liquid chromatographic separation methods, in Comprehensive Natural Products II: Chemistry and Biology, eds Hung-wen Liu and Lew Mander, Elsevier, Oxford England, pp. 5-49.

Hennsey, T.P., Huber, M.I., Unger, K.K., Boysen, R.I., Hearn, M.T.W., 2003, Separation of peptides and proteins by high performance capillary electrochromatography (HPCEC), in Proteins and Proteomics: A laboratory manual, Techniques section, Technique 4, eds Richard J. Simpson, Cold Spring Harbor Laboratory Press, USA, pp. 862-868.

Boysen, R.I., Hearn, M.T.W., 2001, HPLC of Peptides and Proteins, in Current Protocols in Protein Science, eds J E Coligan, B M Dunn, D W Speicher, P T Wingfield, John Wiley & Sons, USA, pp. 1-40.

Boysen, R.I., Hearn, M.T.W., 2001, HPLC of Peptides and Proteins: Standard Operating Conditions, in Current Protocols in Molecular Biology, eds F M Ausubel, R Brent, R E Kingston, D D Moore, J G Seidman, J A Smith, K Struhl, John Wiley & Sons, USA, pp. 1-32.

Boysen, R.I., Hearn, M.T.W., 2001, HPLC of peptides and proteins: standard operating conditions, in Current Protocols in Molecular Biology, eds Lisa Albright, Donald Coen, Ajit Varki, John Wiley & Sons, Inc, USA, pp. 10.13.1-10.13.32.

Boysen, R.I., Hearn, M.T.W., 2001, Purification of Proteins by High-Performance Liquid Chromatography, in Current Protocols in Molecular Biology, eds F M Ausubel, R Brent, R E Kingston, D D Moore, J G Seidman, J A Smith, K Struhl, John Wiley and Sons, USA, pp. 1-9.

Boysen, R.I., Hearn, M.T.W., 2001, Purification of proteins by high-performace liquid chromatography. HPLC of peptides and proteins: preparation and system set-up, in Current Protocols in Molecular Biology, eds Lisa Albright, Donald Coen, Ajit Varki, John Wiley & Sons, USA, pp. 10.12.1-10.12.9.

Journal Articles

Komaromy, A.z., Li, S., Zhang, H., Nicolau, D.V., Boysen, R.I., Hearn, M.T.W., 2012, Arrays of nano-structured surfaces to probe the adhesion and viability of bacteria, Microelectronic Engineering [P], vol 91, Elsevier Science BV, Amsterdam Netherlands, pp. 39-43.

Boysen, R.I., Li, S., Chowdhury, J., Schwarz, L., Hearn, M.T.W., 2012, Selectivity optimisation of biomimetic molecularly imprinted polymer thin films, Microelectronic Engineering [P], vol 97, Elsevier Science BV, Amsterdam Netherlands, pp. 81-84.

Schwarz, L., Danylec, B., Yang, Y., Harris, S., Boysen, R., Hearn, M., 2011, Enrichment of (E)-resveratrol from peanut byproduct with molecularly imprinted polymers, Journal of Agricultural and Food Chemistry [P], vol 59, issue 8, American Chemical Society, Washington Detroit USA, pp. 3539-3543.

Schwarz, L., Danylec, B., Harris, S., Boysen, R., Hearn, M., 2011, Preparation of molecularly imprinted polymers for the selective recognition of the bioactive polyphenol, (E)-resveratrol, Journal of Chromatography A [P], vol 1218, issue 16, Elsevier Science BV, Amsterdam Netherlands, pp. 2189-2195.

Boysen, R., Yang, Y., Chowdhury, J., Matyska, M., Pesek, J., Hearn, M., 2011, Simultaneous separation of hydrophobic and hydrophilic peptides with a silica hydride stationary phase using aqueous normal phase conditions, Journal of Chromatography A [P], vol 1218, issue 44, Elsevier Science BV, Amsterdam Netherlands, pp. 8021-8026.

Li, S., Komaromy, A.z., Nicolau, D.V., Boysen, R.I., Hearn, M.T.W., 2010, Bacterial adhesion to toroidal nano-structures from poly(styrene)-block-poly(tert-butyl acrylate) diblock copolymer thin films, Microelectronic Engineering [P], vol 87, issue 5-8, Elsevier Science BV, Amsterdam Netherlands, pp. 715-718.

Alam, A., Mataj, A., Yang, Y., Boysen, R., Bowden, D., Hearn, M., 2010, Rapid microwave-assisted chemical cleavage - mass spectrometric method for the identification of hemoglobin variants in blood, Analytical Chemistry [P], vol 82, issue 21, American Chemical Society, Washington DC USA, pp. 8922-8930.

Kazi, S., Kelso, G., Harris, S., Boysen, R., Chowdhury, J., Hearn, M., 2010, Synthesis of quinoline thioethers as novel small molecule enhancers of monoclonal antibody production in mammalian cell culture, Tetrahedron [P], vol 66, issue 48, Pergamon Elsevier Science Ltd, Oxford England, pp. 9461-9467.

Pesek, J.J., Matyska, M., Hearn, M.T.W., Boysen, R.I., 2009, Aqueous normal-phase retention of nucleotides on silica hydride columns, Journal of Chromatography A [P], vol 1216, issue 7, Elsevier BV, Netherlands, pp. 1140-1146.

Held, M., Komaromy, A.z., Fulga, F., Edwards, C., Boysen, R.I., Hearn, M.T.W., Nicolau, D.V., 2009, Dynamic behaviour of microorganisms on microstructures, Microelectronic Engineering [P], vol 86, Elsevier BV, Netherlands, pp. 1455-1458.

Yang, Y., Boysen, R.I., Hearn, M.T.W., 2009, Hydrophilic interaction chromatography coupled to electrospray mass spectrometry for the separation of peptides and protein digests, Journal of Chromatography A [P], vol 1216, issue 29, Elsevier BV, Netherlands, pp. 5518-5524.

Komaromy, A.z., Boysen, R.I., Zhang, H., McKinnon, I.R., Fulga, F., Hearn, M.T.W., Nicolau, D., 2009, Micro-structures modulate bacterial cell viability and attachment, Microelectronic Engineering [P], vol 86, issue 4, Elsevier BV, Netherlands, pp. 1431-1434.

Yang, Y., Boysen, R.I., Harris, S.J., Hearn, M.T.W., 2009, Peptide mapping with mobile phases of intermediate pH value using capillary reversed-phase high-performance liquid chromatography/electrospray ionisation tandem mass spectrometry, Journal of Chromatography A [P], vol 1216, issue 18, Elsevier BV, Netherlands, pp. 3767-3773.

Wang, Y., Boysen, R.I., Wood, B.R., Kansiz, M., McNaughton, D., Hearn, M.T.W., 2008, Determination of the secondary structure of proteins in different environments by FTIR-ATR spectroscopy and PLS regression, Biopolymers, vol 89, issue 11, Wiley Interscience, USA, pp. 895-905.

Foulds, L.M., Boysen, R.I., Crane, M., Yang, Y., Muir, J.A., Smith, A.I., de Kretser, D.M., Hearn, M.T.W., Hedger, M.P., 2008, Molecular Identification of lyso-glycerophosphocholines as endogenous immunosuppressives in bovine and rat gonadal fluids, Biology of Reproduction, vol 79, issue 3, Society for the Study of Reproduction, United States, pp. 525-536.

Yang, Y., Boysen, R.I., Matyska, M., Pesek, J.J., Hearn, M.T.W., 2007, Open-Tubular capillary electrochromatography coupled with electrospray ionization mass spectrometry for peptide analysis, Analytical Chemistry, vol 79, issue 13, American Chemical Society, USA, pp. 4942-4949.

Pesek, J.J., Matyska, M., Hearn, M.T.W., Boysen, R.I., 2007, Temperature effects on solute retention for hydride-based stationary phases, Journal of Separation Science, vol 30, Wiley - V C H Verlag GmbH & Co. KGaA, Germany, pp. 1150-1157.

Boysen, R., Hearn, M., 2006, Desalting of peptides and protein mixtures by RP-HPLC techniques, Cold Spring Harbor Protocols [E], vol 5, Cold Spring Harbor Laboratory, USA, pp. 1-6.

Yang, Y., Boysen, R.I., Hearn, M.T.W., 2006, Optimization of field-amplified sample injection for analysis of peptides by capillary electrophoresis-mass spectrometry, Analytical Chemistry, vol 78, issue 14, American Chemical Society, USA, pp. 4752-4758.

Boysen, R., Hearn, M., 2006, Purification of peptides from solid-phase peptide synthesis with RP-HPLC, Cold Spring Harbor Protocols [E], vol 5, Cold Spring Harbor Laboratory, USA, pp. 1-9.

Boysen, R.I., Jong, A.J.O., Hearn, M.T.W., 2005, Binding behaviour and conformational properties of globular proteins in the presence of immobilised non-polar ligands used in reversed-phase liquid chromatography, Journal of Chromatography A, vol 1079, Elsevier Science BV, Amsterdam The Netherlands, pp. 173-186.

Matyska, M., Pesek, J.J., Chen, J.I., Boysen, R.I., Hearn, M.T.W., 2005, Probing with synthetic peptides the interactive properties of N-butylphenyl and cholesterol-10-undecenaoate phases immobilised onto open tubular electrochromatographic capillaries, Chromatographia, vol 61, issue 7/8, Vieweg, Germany, pp. 351-357.

Yang, Y., Boysen, R.I., Hearn, M.T.W., 2005, Use of mixed-mode sorbents for the electrochromatographic separation of thrombin receptor antagonistic peptides, Journal of Chromatography A, vol 1079, issue 1-Feb, Elsevier Science BV, Amsterdam The Netherlands, pp. 328-334.

Yang, Y., Boysen, R.I., Hearn, M.T.W., 2004, Analysis of synthetic peptides by capillary electrophoresis: effect of organic solvent modifiers and variable electrical potentials on separation efficiencies, Journal of Chromatography A, vol 1043, Elsevier, Amsterdam The Netherlands, pp. 91-97.

Yang, Y., Boysen, R.I., Hearn, M.T.W., 2004, Impact of organic solvents on the resolution of synthetic peptides by capillary electrophoresis, Journal of Chromatography A, vol 1043, Elsevier, Amsterdam The Netherlands, pp. 81-89.

Pesek, J.J., Matyska, M., Dawson, G.B., Chen, J.I., Boysen, R.I., Hearn, M.T.W., 2004, Open tubular capillary electrochromatography of synthetic peptides on etched chemically modified columns, Analytical Chemistry, vol 76, issue 1, American Chemical Society, Washington USA, pp. 23-30.

Pesek, J.J., Matyska, M., Brent-Dawson, G., Chen, J.I., Boysen, R.I., Hearn, M.T.W., 2004, Open-tubular electrochromatographic characterization of synthetic peptides, Electrophoresis, vol 25, Wiley, Germany, pp. 1211-1218.

Pesek, J.J., Matyska, M., Dawson, G.B., Chen, J.I., Boysen, R.I., Hearn, M.T.W., 2003, Open tubular capillary electrochromatography of synthetic peptides on etched chemically modified columns, Analytical Chemistry, vol 76, issue 1, American Chemical Society, Washington USA, pp. 23-30.

Hennessy, T., Boysen, R.I., Huber, M.I., Unger, K.K., Hearn, M.T.W., 2003, Peptide mapping by reversed-phase high-performance liquid chromatography employing silica rod monoliths, Journal of Chromatography A, vol 1009, Elsevier, Amsterdam The Netherlands, pp. 15-28.

Yang, Y., Boysen, R.I., Chen, J.I., Keah, H.H., Hearn, M.T.W., 2003, Separation of structurally related synthetic peptides by capillary zone electrophoresis, Journal of Chromatography A, vol 1009, Elsevier, Amsterdam The Netherlands, pp. 3-14.

Walhagen, K., Boysen, R.I., Hearn, M.T.W., Unger, K.K., 2003, The CEC Behaviour of several synthetic peptides related to the activin beta A - beta D subunits, International Journal of Peptide Research and Therapeutics, vol 61, Springer, New York NY USA, pp. 109-121.

Chowdury, J., Boysen, R.I., Ihara, H., Hearn, M.T.W., 2002, Binding Behaviour of Crystalline and noncrystalline Phases: Evaluation of the Enthalpic and Entropic Contributions to the Separation Selectivity of Nonpolar Solutes with a Novel Chromatographic Sorbent, Journal of Physical Chemistry B [P], vol 106, American Chemical Society, Washington DC USA, pp. 11936-11950.

Boysen, R.I., Jong, A., Wilce, J.A., King, G.F., Hearn, M.T.W., 2002, Role of Interfacial Hydrophobic Residues in the Stabilization of the Leucine Zipper structures of the transcription Factors c-Fos and c-Jun, The Journal of Biological Chemistry, vol 277, issue 1, The American Society for Biochemistry and Molecular Biology I, Bethesda MD USA, pp. 23-31.

Boysen, R.I., Jong, A., Hearn, M.T.W., 2002, Thermodynamic Assessment of the Stability of Thromic Receptor Antagonistic Peptides in Hydrophobic Environments, Biophysical Journal, vol 82, Biophysical Society, Bethesda MD USA, pp. 2279-2292.

Matyska, M., Pesek, J.J., Boysen, R.I., Hearn, M.T., 2001, Characterization and applications of etched chemically modified capillaries for open-tubular capillary electrochromatography, Electrophoresis, vol 22, Wiley - VCH Verlag GMBH, Berlin Germany, pp. 2620-2628.

Matyska, M., Pesek, J.J., Boysen, R.I., Hearn, M.T., 2001, Characterization of open tubular capillary electrochromatography columns for the analysis of synthetic peptides using isocratic conditions, Analytical Chemistry, vol 73, issue 21, American Chemical Society, Washington DC USA, pp. 5116-5125.

Matyska, M., Pesek, J.J., Boysen, R.I., Hearn, M.T., 2001, Electrochromatographic characterization of etched chemically-modified capillaries with small synthetic peptides, Journal of Chromatography A, vol 924, issue 1-2, Elsevier Science BV, Amsterdam Netherlands, pp. 211-221.

Boysen, R.I., Hearn, M.T., 2001, The metal binding properties of the CCCH motif of the 50S ribosomal protein L36 from Thermus thermophilus, Journal of Peptide Research, vol 57, Munksgaard International Publishing Ltd, Copenhagen Denmark, pp. 19-28.

Keah, H., Allen, N.S., Clay, R.L., Boysen, R.I., Warner, T., Hearn, M.T., 2001, Total chemical synthesis of human activin bA [12-116] and related large-loop polypeptides, Biopolymers, vol 60, issue 4, John Wiley & Sons Inc, Chichester England, pp. 279-289.

Hearn, M.T.W., Keah, H., Boysen, R.I., Messana, I., Misiti, F., Rossetti, D.V., Giardina, B., Castagnola, M., 2000, Determination of biophysical parameters of polypeptide retro-inverso isomers and their analogues by capillary electrophoresis, Analytical Chemistry, vol 72 issue 9, American Chemical Society, Washington USA, pp. 1964-1972.

Boysen, R.I., Hearn, M.T., 2000, Direct characterisation by electrospray ionisation mass spectroscopy of mercuro-polypeptide complexes after deprotection of acetamidomethyl groups from protected cycteine residues of synthetic polypeptides, Journal of Biochemical and Biophysical Methods, vol 45, Elsevier Science BV, Amsterdam Netherlands, pp. 157-168.

Boysen, R.I., Wang, Y., Keah, H., Hearn, M.T., 1999, Observations on the origin of the non-linear Van't Hoff behaviour of polypeptides in hydrophobic environments, Biophysical Chemistry, vol 77 issue 2-3, Elsevier, Amsterdam Netherlands, pp. 79-97.

Aguilar, M., Clayton, D.J., Holt, P., Kronina, V., Boysen, R.I., Purcell, A.W., Hearn, M.T., 1998, RP-HPLC binding domains of proteins, Analytical Chemistry, vol 70 no 23, American Chemical Society, Boston USA, pp. 5010-5018.

Boysen, R.I., Erdmann, V.A., Hearn, M.T., 1998, Systematic, computer-assisted optimisation of the isolation of theremus thermophilus 50S ribosomal proteinsby reversed phase high performance liquid chromatography, Journal of Biochemical and Bioophysical Methods, vol 37, Elsevier Science B.V., Shannon Ireland, pp. 69-89.

Conference Proceedings

Nguyen, M., Chaffee, A.L., Held, M., Boysen, R.I., Nicolau, D., Hearn, M.T., 2008, A study on the atomic hydrophobicity of peptides in aqueous solutions using molecular dynamics modeling methods, Biomedical Applications of Micro- and Nanoengineering IV and Complex Systems, 10 December 2008 to 12 December 2008, SPIE - International Society for Optical Engineering, United States, pp. 727002-1-727002-10.

Komaromy, A., Boysen, R., Zhang, H., Hearn, M., Nicolau, D., 2008, Influence of surface nanostructure on the extent of colonization and cell viability of E. coli and S. aureus, Biomedical Applications of Micro- and Nanoengineering IV and Complex Systems, 09 December 2008 to 12 December 2008, SPIE The International Society Optical Engineering, USA, pp. 727006-1-727006-8.

Li, S., Komaromy, A., Boysen, R., Nicolau, D., Hearn, M., 2008, Self-assembled diblock copolymer thin films for the analysis of bacteria-surface interactions, Biomedical Applications of Micro- and Nanoengineering IV and Complex Systems, 09 December 2008 to 12 December 2008, SPIE The International Society Optical Engineering, USA, pp. 727008-1-727008-8.

Zhang, H., Boysen, R., Sune, G., Borrise, X., Perez-Murano, F., Hearn, M., Nicolau, D., 2008, The effect of hydrophobicity of micro/nanostructured-surfaces on behaviours of water spreading, Biomedical applications of micro- and nanoengineering IV and complex systems, 09 December 2008 to 12 December 2008, SPIE The International Society Optical Engineering, USA, pp. 727009-1-727009-8.

Zhang, H., Komaromy, A.z., Boysen, R.I., Rius, G., Borrise, X., Perez-Murano, F., Hearn, M.T.W., Nicolau, D.V., 2007, Atomic force microscopy study on the attachment of E. coli and S. aureus to a patterned surface of different materials, Proceedings of SPIE: BioMEMS and Nanotechnology III, 04/12/2007-07/12/2007, SPIE, USA, pp. 679903-679912.

Komaromy, A.z., Boysen, R.I., Zhang, H., Hearn, M.T.W., Nicolau, D.V., 2007, Effect of various artificial surfaces on the colonization and viability of E. coli and S. aureus, Proceedings of SPIE, BioMEMS and Nanotechnology III, 04/12/2007-07/12/2007, SPIE, USA, pp. 67990-67999.

Livingston, P., Tsuzuki, T., Boysen, R., Nicolau, D., 2007, Lateral force contrast for the detection of hydrophilic beads embedded within a PDMS surface, Proceedings of the Society of Photo-Optical Instrumentation Engineers (SPIE), 11 December 2006 to 13 December 2006, SPIE International Society Optical Engineering, USA, p. 41604.

Hearn, M.T., Boysen, R.I., Wang, Y., 1998, Entropy-enthalpy compensation effects in the molecular chromatography of polypeptide hormones in lipophilic environments, 18th International Symposium on Separation of Proteins, Peptides & Polynucleotides, Vienna Austria, Nov 1-4, 1998, http://www.boku.ac.at/iam/isppp98/, Vienna Austria, p. 37.

Hearn, M.T., Boysen, R.I., Wang, Y., 1998, Entropy-enthalpy compensation effects in the molecular chromatography of synthetic polypeptide hormones, 3rd Australian Peptide Conference "From Discovery to Therapeutics", Laguna Quays The Whitsundays QLD Oct. 4-9, 1998, Pink Panther Printing, Melbourne Vic Australia, p. 74.

Hearn, M.T., Boysen, R.I., Muraledaram, M., Jong, A., 1998, On the binding behaviour and conformational properties of Polypeptides and gobular proteins in hydrophobic environments, 18th International Symposium on Separation of Proteins, Peptides & Polynucleotides, Vienna Austria Nov 1-4, 1998, COME IN Congress-Meeting-Incentive Organisation, Vienna Austria.

Hearn, M.T., Boysen, R.I., Muralahajan, M., Jong, A., 1998, On the binding behaviour and conformational properties of polypeptides and globular proteins with non-polar ligates, 3rd Australian Peptide Conference "From Discovery to Therapeutics", Laguna Quays The Whitsundays QLD Oct 4-9, 1998, Pink Panther Printing, Melbourne Vic Australia, p. 74.

Hearn, M.T., Boysen, R.I., Wang, Y., 1998, On the binding behaviour and conformational properties of polypeptides and globular proteins with non-polar ligates, 3rd Australian Peptide Conference "From Discovery to Therapeutics", Laguna Quays Queensland, 4-9 October 1998, Pink Panther Printing, Melbourne Vic Australia, p. 74.

Hearn, M.T., Boysen, R.I., Wang, Y., Higgins, K., Keah, H., 1998, Polypeptide topomers: biophysical studies on their conformational and binding behaviour in hydrophobic environments, 3rd Australian Peptide Conference "From Discovery to Therapeutics", Laguna Quays The Whitsundays QLD Oct 4-9, 1998, Pink Panther Printing, Melbourne Vic Australia, p. 95.

Hearn, M.T., Boysen, R.I., Muraladaran, M., Wang, Y., 1998, Thermodynamic and extra-thermodynamic dependencies in the retention behaviors of polypeptides in reversed phase HPLC, 22nd International Symposium on High Performance Liquid Phase Separations and Related Techniques, St Louis U S A, May 2-8, 1998, Barr Enterprises, Walkersville Maryland USA, p. 43.

Hearn, M.T., Boysen, R.I., Muralahajan, M., 1997, Capillary electrophoresis as a tool to assess the conformational status and ligand binding behaviour of peptides and proteins: theoretical and practical considerations, 10th International Symposium on High Performance Capillary Electrophoresis, Kyoto, Japan, July 8-11, 1997, Elsevier Science B.V., Amsterdam Netherlands, pp. 53-54.

Wei, J., Graham, B., Wirth, H.-., Boysen, R.I., Spiccia, L., Hearn, M.T., 1997, Engineering ligands for the high resolution analysis and purification of recombinant proteins, 17th International Symposium on the Separation of Proteins, Peptides and Polynucleotides, Rockvile, Maryland USA, Oct. 26-29, 1997, Barr Enterprises, Rockville MD USA, p. 41.

Clay, R., Boysen, R.I., Hearn, M.T., 1997, Investigation of the a2 - macroglobulin binding regions of Activin bA, bB, bC and bD variants using synthetic peptides, Proceedings, 22nd Annual Lorne Conference on Protein Structure and Function, Lorne, Australia, Feb. 9-13, 1997, Lorne Protein Workshop Inc., Melbourne Vic Australia, p. B54.

Hearn, M.T., Boysen, R.I., Muralahajan, M., Wang, Y., 1997, Investigations into the origin of the deviations from ideal retention behavior of peptides with n-Alkysilica sorbents, 17th International Symposium on the Separation of Proteins, Peptides and Polynucleotides, Rockville, Maryland, USA, Oct. 26-29, 1997, Barr Enterprises, Rockville MD USA, p. 59.

Hearn, M.T., Boysen, R.I., Wang, Y., Muraledaran, M., 1997, Peptide conformational behaviour studied by high and low temperature HPLC and HPCE, 1st International Peptide Symposium, Kyoto, Japan, Nov. 30-Dec. 5, 1997, Kluwer Academic Publishers B.V., Amsterdam Netherlands, p. 67.

Boysen, R.I., Keah, H., Wang, Y., Hearn, M.T., 1997, Studies on the retention behaviour and conformational stability of all L-a- and their retro - inversion all D-a - peptide isomer pairs using reversed - phase high - performance liquid chromatography, Proceedings, 22nd Annual Lorne Conference on Protein Structure and Function, Lorne, Australia, Feb. 9-13, 1997, Lorne Protein Workshop Inc., Melbourne Vic Australia, p. A88.

Other

Hearn, M., Langford, S., Tuck, K., Harris, S., Boysen, R., Perchyonok, V., Danylec, B., Schwarz, L., Chowdhury, J., 2010, Moleculary Imprinted Polymers, Melbourne, Victoria, Australia.

Alam, M.A., Bowden, D.K., Boysen, R.I., Hearn, M.T.W., 2005, A method for the rapid analysis of polypeptides, Australia.

Boysen, R.I., Hearn, M.T.W., 2003, Desalting of peptides and protein mixtures by RP-HPLC techniques, Proteins and Proteomics: A laboratory manual, Simpson, R. (ed.), Chapter 5, Reversed-phase high-performance liquid chromatography, Protocol 5, Cold Spring Harbor Laboratory Press, USA.

Boysen, R.I., Hearn, M.T.W., 2003, Purification of peptides from solid phase peptide synthesis with reversed-phase high-performance liquid chromatography, Proteins and Proteomics: A laboratory manual, Simpson, R. (ed.), Chapter 5, Reversed-phase high-performance liquid chromatography, Protocol 4, Cold Spring Harbor Laboratory Press, USA.

Boysen, R.I., Hearn, M.T.W., 2003, Separation of proteins with reversed-phase high-performance liquid chromatography using a computer assisted method to optimise gradient conditions, Proteins and Proteomics: A laboratory manual, Simpson, R. (ed.), Chapter 5, Reversed-phase high-performance liquid chromatography, Protocol 6, Cold Spring Harbor Laboratory Press, USA.

Postgraduate Research Supervisions

Current Supervision

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Advanced analysis of polyphenols and phytosterols in grapes and grape by products.

Supervisors:

Boysen, R (Joint), Hearn, M (Joint-co).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Capillary electrophoresis separation and high-throughput proteomics analysis of recombinant proteins.

Supervisors:

Hearn, M (Main), Boysen, R (Associate), Harris, S (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Comparison of different smart polymers for natural product separation.

Supervisors:

Saito, K (Main), Boysen, R (Associate), Hearn, M (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Investigation of Butyrolactone like Small Molecules in Regulation of Antibiotic Production in Streptomyces.

Supervisors:

Boysen, R (Joint), Hearn, M (Joint-co).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Investigation of protein-ligand interactions in liquid chromatographic systems.

Supervisors:

Boysen, R (Joint-co), Hearn, M (Joint).

Completed Supervision

Student:

Alam, A.

Program of Study:

High resolution mass spectrometry based analysis of biological samples. (PHD) 2008.

Supervisors:

Bowden, D (Main), Hearn, M (Associate), Boysen, R (Associate).

Student:

Banti, Y.

Program of Study:

Novel molecularly imprinted polymers (MIPs) mimicking the catalytic activity of trypsin. (PHD) 2008.

Supervisors:

Hearn, M (Main), Boysen, R (Associate).

Student:

Chen, J.

Program of Study:

Analysis of glycosylated biomolecules. (PHD) 2006.

Supervisors:

Hearn, M (Main), Boysen, R (Associate).

Student:

Jong, A.

Program of Study:

THERMODYNAMIC ANALYSIS OF THE INTERACTION OF ALPHA-HELICAL POLYPEPTIDES AND PROTEINS WITH NONPOLAR LIGANDS IN REVERSED-PHASE HPLC. (PHD) 2003.

Supervisors:

Hearn, M (Main), Boysen, R (Associate).

Student:

Mataj, A.

Program of Study:

A new approach to synthesis and application of novel classes of ligands for the affinity capture and characterisation of phosphorylated peptides. (PHD) 2010.

Supervisors:

Hearn, M (Main), Boysen, R (Associate).

Student:

Wang, Y.

Program of Study:

INVESTIGATIONS INTO PROTEIN CONFORMATIONAL CHANGES IN HYDROPHOBIC INTERACTION ENVIRONMENTS. (PHD) 2003.

Supervisors:

Hearn, M (Main), Hearn, M (Main), Boysen, R (Associate).

Student:

Yin, Y.

Program of Study:

Experimental investigation into biomolecule-ligand interaction. (PHD) 2007.

Supervisors:

Hearn, M (Main), Boysen, R (Associate).