Dr John Boyce - Researcher Profile

Biography

John works in the Faculty of Medicine, Nursing & Health Sciences at Monash University as a Senior Lecturer.

Qualifications

PHD (BIOCHEMISTRY)

Institution: University of Melbourne

Year awarded: 2005

BACHELOR OF SCIENCE (HONOURS)

Institution: Melbourne University

Year awarded: 1994

Publications

Book Chapters

Boyce, J.D., Harper, M., Wilkie, I., Adler, B., 2010, Pasteurella, in Pathogenesis of Bacterial Infections in Animals, eds C.L. Gyles, J.F. Prescott, J. Glenn Songer, C.O. Thoen, Blackwell Publishing (John Wiley & Sons), USA, pp. 325-346.

Boyce, J.D., Adler, B., 2008, Proteomic analyses in Pasteurellaceae, in Pasteurellaceae: Biology, Genomics and Molecular Aspects, eds H ChristensenP Kuhnert, Caister Academic Press, UK, pp. 99-112.

Boyce, J.D., Lo, R.Y.C., Wilkie, I.W., Adler, B., 2004, Pasteurella and Mannheimia, in Pathogenesis of Bacterial Infections in Animals, eds Carlton L Gyles, John F Prescott, J Glenn Songer and Charles O Thoen, Blackwell Publishing Ltd, Oxford England, pp. 273-294.

Journal Articles

Li, X., Prescott, M., Adler, B., Boyce, J.D., Devenish, R.J., 2013, Beclin 1 is required for starvation-enhanced, but not rapamycin-enhanced, LC3-associated phagocytosis of Burkholderia pseudomallei in RAW 264.7 cells, Infection And Immunity [P], vol 81, issue 1, American Society for Microbiology, USA, pp. 271-277.

Soon, R.L., Li, J., Boyce, J.D., Harper, M., Adler, B., Larson, I.C., Nation, R.L., 2012, Cell surface hydrophobicity of colistin-susceptible vs resistant Acinetobacter baumannii determined by contact angles: methodological considerations and implications, Journal Of Applied Microbiology [P], vol 113, issue 4, Wiley-Blackwell Publishing Ltd., United Kingdom, pp. 940-951.

Harper, M., St Michael, F., Vinogradov, E., John, M., Boyce, J.D., Adler, B., Cox, A.D., 2012, Characterization of the lipopolysaccharide from Pasteurella multocida Heddleston serovar 9: identification of a proposed bi-functional dTDP-3-acetamido-3,6-dideoxy-alpha-D-glucose biosynthesis enzyme, Glycobiology [P], vol 22, issue 3, Oxford University Press, USA, pp. 332-344.

Henry, R.M., Vithanage (Sulthanagoda), N.R., Harrison, P., Seemann, T., Coutts, S.A., Moffatt, J.H., Nation, R.L., Li, J., Harper, M., Adler, B., Boyce, J.D., 2012, Colistin-resistant, lipopolysaccharide-deficient Acinetobacter baumannii responds to lipopolysaccharide loss through increased expression of genes involved in the synthesis and transport of lipoproteins, phospholipids, and poly-, Antimicrobial Agents And Chemotherapy [P], vol 56, issue 1, American Society for Microbiology, USA, pp. 59-69.

Kristensen, B.M., Sinha, S., Boyce, J.D., Bojesen, A.M., Mell, J.C., Redfield, R.J., 2012, Natural transformation of gallibacterium anatis, Applied And Environmental Microbiology [P], vol 78, issue 14, American Society for Microbiology, USA, pp. 4914-4922.

Wilkie, I.W., Harper, M., Boyce, J.D., Adler, B., 2012, Pasteurella multocida: diseases and pathogenesis, Current Topics In Microbiology And Immunology [P], vol 361, Springer New York LLC, USA, pp. 1-22.

Boyce, J.D., Seemann, T., Adler, B., Harper, M., 2012, Pathogenomics of pasteurella multocida, Current Topics In Microbiology And Immunology [P], vol 361, Springer New York LLC, USA, pp. 23-38.

Hatfaludi, T.Z., Al-Hasani, K., Gong, L., Boyce, J.D., Ford, M., Wilkie, I.W., Quinsey, N.S., Dunstone, M.A., Hoke, D.E., Adler, B., 2012, Screening of 71 P. multocida proteins for protective efficacy in a fowl cholera infection model and characterization of the protective antigen PlpE, PLoS ONE [P], vol 7, issue 7 (Art. ID: e39973), Public Library of Science, USA, pp. 1-11.

Harper, M., Boyce, J.D., Adler, B., 2012, The key surface components of pasteurella multocida: capsule and lipopolysaccharide, Current Topics In Microbiology And Immunology [P], vol 361, Springer New York LLC, USA, pp. 39-51.

Soon, R., Nation, R., Cockram, S., Moffatt, J., Harper, M., Adler, B., Boyce, J., Larson, I., Li, J., 2011, Different surface charge of colistin-susceptible and -resistant acinetobacter baumannii cells measured with zeta potential as a function of growth phase and colistin treatment, Journal Of Antimicrobial Chemotherapy [P], vol 66, issue 1, Oxford University Press, United Kingdom, pp. 126-133.

Soon, R., Nation, R., Harper, M., Adler, B., Boyce, J., Tan, C., Li, J., Larson, I., 2011, Effect of colistin exposure and growth phase on the surface properties of live acinetobacter baumannii cells examined by atomic force microscopy, International Journal of Antimicrobial Agents [P], vol 38, issue 6, Elsevier, Netherlands, pp. 493-501.

Moffatt, J.H., Harper, M., Adler, B., Nation, R.L., Li, J., Boyce, J.D., 2011, Insertion sequence ISAba11 is involved in colistin resistance and loss of lipopolysaccharide in Acinetobacter baumannii, Antimicrobial Agents And Chemotherapy [P], vol 55, issue 6, American Society for Microbiology, USA, pp. 3022-3024.

Bannam, T.L., Yan, X., Harrison, P.F., Seemann, T., Keyburn, A.L., Stubenrauch, C., Weeramantri, L.H., Cheung, J.K., McClane, B.A., Boyce, J.D., Moore, R.J., Rood, J.I., 2011, Necrotic enteritis-derived Clostridium perfringens strain with three closely related independently conjugative toxin and antibiotic resistance plasmids, mBio [E], vol 2, issue 5 (Art. No: e00190), American Society for Microbiology, USA, pp. 1-9.

Harper, M., St Michael, F., John, M., Vinogradov, E., Adler, B., Boyce, J.D., Cox, A.D., 2011, Pasteurella multocida Heddleston serovars 1 and 14 express different lipopolysaccharide structures but share the same lipopolysaccharide biosynthesis outer core locus, Veterinary Microbiology [P], vol 150, issue 3-4, Elsevier BV, The Netherlands, pp. 289-296.

Harper, M., Cox, A., Adler, B., Boyce, J., 2011, Pasteurella multocida lipopolysaccharide: The long and the short of it, Veterinary Microbiology [P], vol 153, issue 1-2, Elsevier Science BV, The Netherlands, pp. 109-115.

D'Cruze, T., Gong, L., Treerat, P., Ramm, G., Boyce, J., Prescott, M., Adler, B., Devenish, R., 2011, Role for the Burkholderia pseudomallei Type Three Secretion System Cluster 1 bpscN gne in vrulence, Infection And Immunity [P], vol 79, issue 9, American Society for Microbiology, USA, pp. 3659-3664.

Allwood, E., Devenish, R., Prescott, M., Adler, B., Boyce, J., 2011, Strategies for Intracellular Survival of Burkholderia pseudomallei, Frontiers in Microbiology [E], vol 2, issue Art. No: 170, Frontiers in Research Foundation, Switzerland, pp. 1-19.

Gong, L., Cullinane, M., Treerat, P., Ramm, G., Prescott, M., Adler, B., Boyce, J.D., Devenish, R.J., 2011, The Burkholderia pseudomallei type III secretion system and BopA are required for evasion of LC3-associated phagocytosis, PLoS ONE [P], vol 6, issue 3 (Art. No: e17852), Public Library of Science, USA, pp. 1-11.

Moffatt, J.H., Harper, M., Harrison, P.F., Hale, J.D., Vinogradov, E., Seemann, T., Henry, R.M., Crane, B.A., St Michael, F., Cox, A.D., Adler, B., Nation, R.L., Li, J., Boyce, J.D., 2010, Colistin resistance in Acinetobacter baumannii is mediated by complete loss of lipopolysaccharide production, Antimicrobial Agents And Chemotherapy [P], vol 54, issue 12, American Society for Microbiology, USA, pp. 4971-4977.

Lo, A., Seers, C.A., Dashper, S.G., Butler, C., Walker, G., Walsh, K., Catmull, D., Hoffmann, B., Cleal, S., Lissel, P., Boyce, J.D., Reynolds, E.C., 2010, FimR and FimS: biofilm formation and gene expression in Porphyromonas gingivalis, Journal Of Bacteriology [P], vol 192, issue 5, American Society for Microbiology, USA, pp. 1332-1343.

Steen, J.A., Steen, J.A., Harrison, P.F., Seemann, T., Wilkie, I.W., Harper, M., Adler, B., Boyce, J.D., 2010, Fis is essential for capsule production in Pasteurella multocida and regulates expression of other important virulence factors, Plos Pathogens [P], vol 6, issue 2, Public Library of Science, USA, pp. e1000750-1-e1000750-14.

Harper, M., Cox, A.D., St Michael, F., Ford, M., Wilkie, I.W., Adler, B., Boyce, J.D., 2010, Natural selection in the chicken host identifies 3-deoxy-D-manno- octulosonic acid kinase residues essential for phosphorylation of Pasteurella multocida lipopolysaccharide, Infection And Immunity [P], vol 78, issue 9, American Society for Microbiology, USA, pp. 3669-3677.

Hatfaludi, T.Z., Al-Hasani, K., Boyce, J.D., Adler, B., 2010, Outer membrane proteins of Pasteurella multocida, Veterinary Microbiology [P], vol 114, issue 1-2, Elsevier BV, The Netherlands, pp. 1-17.

Lo, A.W., Seers, C.A., Boyce, J.D., Dashper, S.G., Slakeski, N., Lissel, J.P., Reynolds, E.C., 2009, Comparative transcriptomic analysis of Porphyromonas gingivalis biofilm and planktonic cells, BMC Microbiology [P], vol 9, issue 18, BioMed Central Ltd, UK, pp. 1-11.

Boyce, J.D., Harper, M., St Michael, F., John, M., Aubry, A., Parnas, H., Logan, S.M., Wilkie, I.W., Ford, M.E., Cox, A.D., Adler, B., 2009, Identification of novel glycosyltransferases required for assembly of the Pasteurella multocida A:1 lipopolysaccharide and their involvement in virulence, Infection and Immunity, vol 77, issue 4, American Society for Microbiology, USA, pp. 1532-1542.

St Michael, F., Harper, M., Parnas, H., John, M., Stupak, J., Vinogradov, E., Adler, B., Boyce, J.D., Cox, A.D., 2009, Structural and genetic basis for the serological differentiation of Pasteurella multocida Heddleston Serotypes 2 and 5, Journal Of Bacteriology [P], vol 191, issue 22, American Society for Microbiology, USA, pp. 6950-6959.

Lazar Adler, N.R., Govan, B., Cullinane, M., Harper, M., Adler, B., Boyce, J.D., 2009, The molecular and cellular basis of pathogenesis in melioidosis: How does Burkholderia pseudomallei cause disease?, FEMS Microbiology Reviews [P], vol 33, issue 6, Wiley-Blackwell Publishing Ltd, UK & The Netherlands, pp. 1079-1099.

Hatfaludi, T.Z., Al-Hasani, K., Dunstone, M.A., Boyce, J.D., Adler, B., 2008, Characterization of TolC efflux pump proteins from Pasteurella multocida, Antimicrobial Agents and Chemotherapy, vol 52, issue 11, American Society for Microbiology, United States, pp. 4166-4171.

Keyburn, A., Boyce, J.D., Vaz, P.K., Bannam, T.L., Ford, M.E., Parker, D., Di Rubbo, A., Rood, J.I., Moore, R.J., 2008, NetB, a new toxin that is associated with avian necrotic enteritis caused by Clostridium perfringens, PL o S Pathogens, vol 4, issue 2 (e26), Public Library of Science, United States, pp. 1-11.

Cullinane, M., Gong, L., Li, X., Lazar Adler, N., Tra, T.C., Wolvetang, E.J., Prescott, M., Boyce, J.D., Devenish, R.J., Adler, B., 2008, Stimulation of autophagy suppresses the intracellular survival of Burkholderia pseudomallei in mammalian cell lines, Autophagy, vol 4, issue 6, Landes Bioscience, United States, pp. 744-753.

Harper, M., Cox, A.D., St Michael, F., Parnas, H., Wilkie, I.W., Blackall, P.J., Adler, B., Boyce, J.D., 2007, Decoration of pasteurella multocida lipopolysaccharide with phosphocholine is important for virulence, Journal of Bacteriology, vol 189, issue 20, American Society of Microbiology, Washington, DC, USA, pp. 7384-7391.

Myers, G.S., Parker, D., Al-Hasani, K., Kennan, R.M., Seemann, T., Ren, Q., Badger, J.H., Selengut, J.D., DeBoy, R.T., Tettelin, H., Boyce, J.D., McCarl, V., Han, X.Y., Nelson, W.C., Madupu, R., Mohamoud, Y., Holley, T., Fedorova, N., Bottomley, S.P., Whittington, R.J., Adler, B., Songer, J., Rood, J.I., Paulsen, I.T., 2007, Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus, Nature Biotechnology, vol 25, issue 5, Nature Publishing Group, New York NY USA, pp. 569-575.

Al-Hasani, K., Boyce, J.D., McCarl, V., Bottomley, S.P., Wilkie, I.W., Adler, B., 2007, Identification of novel immunogens in Pasteurella multocida, Microbial Cell Factories, vol 6, issue 3, BioMed Central Ltd, London England UK, pp. 1-5.

Harper, M., Boyce, J.D., Cox, A.D., St Michael, F., Wilkie, I.W., Blackall, P.J., Adler, B., 2007, Pasteurella multocida expresses two lipopolysaccharide glycoforms simultaneously, but only a single form is required for virulence: Identification of two acceptor-specific heptosyl I transferases, Infection and Immunity, vol 75, issue 8, American Society of Microbiology, Washington DC USA, pp. 3885-3893.

Boyce, J.D., Cullen, P.A., Nguyen, V., Wilkie, I.W., Adler, B., 2006, Analysis of the Pasteurella multocida outer membrane sub-proteome and its response to the in vivo environment of the natural host, Proteomics, vol 6, issue 3, Wiley - VCH Verlag GmbH & Co. KGaA, Germany, pp. 870-880.

Boyce, J.D., Adler, B., 2006, How does Pasteurella multocida respond to the host environment?, Current Opinion in Microbiology, vol 9, issue 1, Current Biology Ltd, LONDON ENGLAND, pp. 117-122.

Harper, M., Boyce, J.D., Adler, B., 2006, Pasteurella multocida pathogenesis: 125 years after Pasteur, FEMS Microbiology Letters, vol 265, issue 1, Blackwell Publishing Ltd, OXON ENGLAND, pp. 1-10.

Boucher, D.J., Adler, B., Boyce, J.D., 2005, The Pasteurella multocida nrfE gene Is upregulated during infection and is essential for nitrite reduction but not for virulence, Journal of Bacteriology, vol 187, issue 7, American Society for Microbiology, USA, pp. 2278-2285.

Chung, J.Y., Wilkie, I.W., Boyce, J.D., Adler, B., 2005, Vaccination against fowl cholera with acapsular Pasteurella multocida A:1, Vaccine, vol 23, Elsevier Ltd, UK, pp. 2751-2755.

Harper, M., Cox, A.D., St Michael, F., Wilkie, I.W., Boyce, J.D., Adler, B., 2004, A heptosyltransferase mutant of Pasteurella multocida produces a truncated lipopolysaccharide structure and Is attenuated in virulence, Infection and Immunity, vol 72, issue 6, American Society for Microbiology, Washington USA, pp. 3436-3443.

Lo, M., Boyce, J.D., Wilkie, I.W., Adler, B., 2004, Characterization of two lipoproteins in Pasteurella multocida, Microbes and Infection, vol 6, issue 1, Elsevier, Amsterdam The Netherlands, pp. 58-67.

Boyce, J.D., Wilkie, I.W., Harper, M., Paustian, M.L., Kapur, V., Adler, B., 2004, Genomic-scale analysis of Pasteurella multocida gene expression during growth within liver tissue of chickens with fowl cholera, Microbes and Infection, vol 6, issue 3, Elsevier, Amsterdam The Netherlands, pp. 290-298.

Boyce, J.D., Cullen, P.A., Adler, B., 2004, Genomic-scale analysis of bacterial gene and protein expression in the host, Emerging Infectious Diseases, vol 10, issue 8, Center Disease Control, Atlanta USA, pp. 1357-1362.

Cox, A.J., Hunt, M.L., Boyce, J.D., Adler, B., 2003, Functional characterization of HgbB, a new hemoglobin binding protein of Pasteurella multocida, Microbial Pathogenesis, vol 34, issue 6, Academic Press Ltd, Elsevier Science Ltd, London UK, pp. 287-296.

Harper, M., Boyce, J.D., Wilkie, I.W., Adler, B., 2003, Signature-tagged mutagenesis of Pasteurella multocida identifies mutants displaying differential virulence characteristics in mice and chickens, Infection and Immunity, vol 71, issue 9, American Soceity for Microbiology, Washington USA, pp. 5440-5446.

Boyce, J.D., Wilkie, I., Harper, M., Paustian, M.L., Kapur, V., Adler, B., 2002, Genomic Scale Analysis of Pasteurella multocida Gene Expression during Growth within the Natural Chicken Host, Infection and Immunity, vol 70, issue 12, ASM, Washington DC USA, pp. 6871-6879.

Boyce, J.D., Adler, B., 2001, Acapsular pasteurella multocida B:2 can stimulate protective immunity against pasteurellosis, Infection and Immunity, vol 69, issue 3, American Society for Microbiology, Washington DC USA, pp. 1943-1946.

Townsend, K.M., Boyce, J.D., Chung, J.Y., Frost, A.J., Adler, B., 2001, Genetic organization of Pasteurella multocida cap Locia and development of a multiplex capsular PCR typing system, Journal of Clinical Microbiology, vol 39, issue 3, American Society for Microbiology, Washington DC USA, pp. 924-929.

Hunt, M.L., Boucher, D., Boyce, J.D., Adler, B., 2001, In vivo-expressed genes of Pasteurella multocida, Infection and Immunity, vol 69, issue 5, American Society for Microbiology, Washington DC USA, pp. 3004-3012.

Chung, J.Y., Wilkie, I., Boyce, J.D., Townsend, K.M., Frost, A.J., Ghoddusi, M., Adler, B., 2001, Role of capsule in the pathogenesis of fowl cholera caused by pasteurella multocida serogroup A, Infection and Immunity, vol 69, issue 4, American Society of Microbiology, Washington DC USA, pp. 2487-2492.

Mahanivong, C., Boyce, J.D., Davidson, B.E., Hillier, A.J., 2001, Sequence analysis and molecular characterization of the Lactococcus lactis temperate bacteriophage BK5-T, Applied and Environmental Microbiology, vol 67, issue 8, American Society for Microbiology, USA, pp. 3564-3576.

Boyce, J.D., Chung, J.Y., Adler, B., 2000, Genetic organisation of the capsule biosynthetic locus of Pasteurella multocida M1404 (B:2), Veterinary Microbiology, vol 72, Elsevier Science BV, Amsterdam Netherlands, pp. 121-134.

Boyce, J.D., Chung, J.Y., Adler, B., 2000, Pasteurella multocida capsule: composition, function and genetics, Journal of Biotechnology, vol 83, Elsevier Science BV, Amsterdam Netherlands, pp. 153-160.

Boyce, J.D., Adler, B., 2000, The capsule is a virulence determinant in the pathogenesis of Pasteurella multocida M1404 (B:2), Infection and Immunity, vol 68 issue 6, American Society for Microbiolgy, Washington USA, pp. 3463-3468.

Postgraduate Research Supervisions

Current Supervision

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Characterisation of a Type VI Secretion System in Acinetobacter baumannii.

Supervisors:

Boyce, J (Main), Harper, M (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Characterisation of two-component signal transfuction systems in Burkholderia pseudomallei.

Supervisors:

Boyce, J (Main), Allwood, E (Associate), Adler, B (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

The mechanism of colistin resistance in A.baumannii.

Supervisors:

Boyce, J (Main), Harper, M (Associate), Li, J (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Type III secretion system (TTSS) and pathogenicity of Burkholderia pseudomallei..

Supervisors:

Adler, B (Joint), Boyce, J (Joint-co).

Completed Supervision

Student:

Harper, M.

Program of Study:

VIRULENCE DETERMINANTS OF PASTEURALLA MULTOCIDA. (PHD) 2003.

Supervisors:

Adler, B (Joint-Co), Boyce, J (Joint).

Student:

Lazar Adler, N.

Program of Study:

Studies on the molecular basis of pathogenesis of melioidosis. (PHD) 2009.

Supervisors:

Boyce, J (Main), Adler, B (Associate), Harper, M (Associate).

Student:

Lo, M.

Program of Study:

CHARACTERISATION OF IN VIVO EXPRESSED PROTEINS OF PASTEURELLA MULTOCIDA. (PHD) 2003.

Supervisors:

Adler, B (Main), Boyce, J (Joint).

Student:

Steen, J.

Program of Study:

Analysis of Pasteurella multocida virulence factors. (PHD) 2010.

Supervisors:

Boyce, J (Joint-Co), Adler, B (Associate), Harper, M (Associate).

Student:

Sulthanagoda Vithanage, N.

Program of Study:

TBD. 2010.

Supervisors:

Boyce, J (Joint-Co), Harper, M (Joint).