Dr Tzong-Hsien Lee - Researcher Profile

Biography

Tzong-Hsien works in the Faculty of Medicine, Nursing & Health Sciences at Monash University as a Research Fellow.

Qualifications

PH. D. (DEPT. BIOCHEMISTRY & MOLECULARY BIOLOGY)

Institution: Monash University

Year awarded: 1999

BACHELOR OF SCIENCE (HONS)- 1ST CLASS

Institution: Monash University

Year awarded: 1994

Publications

Book Chapters

Lee, T., Hall, K.N., Mechler, A.I., Martin, L.L., Popplewell, J., Ronan, G., Aguilar, M.I., 2009, Molecular imaging and orientational changes of antimicrobial peptides in membranes, in Peptides for Youth: The Proceedings of the 20th American Peptide Symposium, eds Susan Del Valle, Emanuel Escher and William D LubeSusan Del Valle, Emanuel Escher and William D Lubell, Springer, USA, pp. 313-315.

Lee, T., Aguilar, M.I., 2008, High performance liquid chromatography (HPLC) of peptides and proteins, in Molecular Biomethods Handbook, eds John M Walker and Ralph Rapley, Humana Press, USA, pp. 767-792.

Lee, T., Aguilar, M.I., 2008, Protein separation using immobilized phospholipid chromatography, in Affinity Chromatography - Methods and Protocols, eds M Zachariou, Humana Press, Totowa NY USA, pp. 295-302.

Lee, T., Aguilar, M., 2006, Trends in the development and application of functional biomembrane surfaces, in Biotechnology Annual Review, Volume 12, eds M Rafaat El-Gewely, Elsevier, Netherlands, pp. 85-136.

Lee, T., Aguilar, M., 2005, HPLC of peptides and proteins, in Encyclopedia of Molecular Cell Biology and Molecular Medicine, eds Robert A Meyers, Wiley-VCH, Weinheim Germany, pp. 245-295.

Lee, T., Aguilar, M.I., 2001, Biomembrane chromatography: Application to purification and biomolecule - membrane interactions, in Advances in Chromoatography Vol 41, eds Phyllis R. Brown and Eli Grushka, Marcel Dekker Inc, New York NY USA, pp. 175-201.

Journal Articles

Fernandez, D.I., Le Brun, A.P., Lee, T., Bansal, P.S., Aguilar, M., James, M., Separovic, F., 2013, Structural effects of the antimicrobial peptide maculatin 1.1 on supported lipid bilayers, European Biophysics Journal With Biophysics Letters [P], vol 42, issue 1, Springer, Germany, pp. 47-59.

Zhai, J., Lee, T., Small, D., Aguilar, M., 2012, Characterization of early stage intermediates in the nucleation phase of A {Beta} aggregation, Biochemistry [P], vol 51, issue 6, American Chemical Society, USA, pp. 1070-1078.

Zhai, J., Hoffmann, S.V., Day, L., Lee, T., Augustin, M., Aguilar, M., Wooster, T., 2012, Conformational changes of a-lactalbumin adsorbed at oil-water interfaces: interplay between protein structure and emulsion stability, Langmuir [P], vol 28, issue 5, American Chemical Society, USA, pp. 2357-2367.

Hall, K.n., Lee, T., Daly, N.L., Craik, D.J., Aguilar, M., 2012, Gly (6) of kalata B1 is critical for the selective binding to phosphatidylethanolamine membranes, Biochimica et Biophysica Acta - Biomembranes [P], vol 1818, issue 9, Elsevier BV, Netherlands, pp. 2354-2361.

Lee, T., Aguilar, M., 2011, Dual polarization interferometry: An optical biosensor which allows new insights into peptide-induced changes in biomembrane structure, Australian Journal of Chemistry [E], vol 64, issue 6, CSIRO Publishing, Australia, pp. 844-845.

Hirst, D., Lee, T., Swann, M.J., Unabia, S., Park, Y., Hahm, K., Aguilar, M.I., 2011, Effect of acyl chain structure and bilayer phase state on binding and penetration of a supported lipid bilayer by HPA3, European Biophysics Journal With Biophysics Letters [P], vol 40, issue 4, Springer, Germany, pp. 503-514.

Zhai, J., Wooster, T., Hoffmann, S., Lee, T., Augustin, M., Aguilar, M., 2011, Structural rearrangement of beta-lactoglobulin at different oil-water interfaces and its effect on emulsion stability, Langmuir [P], vol 27, issue 15, American Chemical Society, USA, pp. 9227-9236.

Zhai, J., Miles, A.J., Pattenden, L.K., Lee, T.-.(., Augustin, M.A., Wallace, B.A., Aguilar, M.-., Wooster, T.J., 2010, Changes in beta-lactoglobulin conformation at the oil/water interface of emulsions studied by synchrotron radiation circular dichroism spectroscopy, Biomacromolecules [P], vol 11, issue 8, American Chemical Society, USA, pp. 2136-2142.

Lee, T., Heng, C., Swann, M.J., Gehman, J.D., Separovic, F., Aguilar, M.I., 2010, Real-time quantitative analysis of lipid disordering by aurein 1.2 during membrane adsorption, destabilisation and lysis, Biochimica et Biophysica Acta - Biomembranes [P], vol 1798, issue 10, Elsevier BV, The Netherlands, pp. 1977-1986.

Lee, T., Hall, K.N., Swann, M.J., Popplewell, J., Unabia, S., Park, Y., Hahm, K., Aguilar, M.I., 2010, The membrane insertion of helical antimicrobial peptides from the N-terminus of Helicobacter pylori ribosomal protein L1, Biochimica et Biophysica Acta - Biomembranes [P], vol 1798, issue 3, Elsevier BV, The Netherlands, pp. 544-557.

Hall, K., Lee, T.-., Aguilar, M.-., 2010, The role of electrostatic interactions in the membrane binding of melittin, Journal Of Molecular Recognition [P], vol 24, issue 1, John Wiley & Sons Ltd, UK & USA, pp. 108-118.

Gehman, J.D., Luc, F., Hall, K.N., Lee, T., Boland, M., Pukala, T.L., Bowie, J.H., Aguilar, M.I., Separovic, F., 2008, Effect of Antimicrobial Peptides from Australian Tree Frogs on Anionic Phospholipid Membranes, Biochemistry, vol 47, issue 33, American Chemical Society, USA, pp. 8557-8565.

Cheng, H., Chang, Y., Chen, Y., Lee, T., Tai, D., Lin, D., 2007, AFP-L3 in chronic liver diseases with persistent elevation of alpha-fetoprotein, Journal of the Chinese Medical Association, vol 70, issue 8, Elsevier (Singapore) Pte Ltd, Hong Kong, pp. 310-317.

Davies, M., Lee, T., Apffel, A., Aguilar, M.I., 2007, Hydrophobic and electrostatic forces control the retention of membrane peptides and proteins with an immobilised phosphatidic acid column, Journal of Chromatography A, vol 1156, issue 1-2, Elsevier Science BV, Amsterdam Netherlands, pp. 167-173.

Lee, T., Tai, D., Cheng, C., Sun, C., Lin, C., Sheu, M., Lee, W., Peng, C., Wang, A.H., Tsai, S., 2006, Enhanced nuclear factor-kappa B-associated Wnt-1 expression in hepatitis B- and C-related hepatocarcinogenesis: identification by functional proteomics, Journal of Biomedical Science, vol 13, issue 1, Springer, Dordrecht The Netherlands, pp. 27-39.

Lin, C., Tsai, S., Lee, T., Chien, R., Liao, S., Liaw, Y., 2005, High frequency of functional anti-YMDD and -mutant cytotoxic T lymphocytes after in vitro expansion correlates with successful response to lamivudine therapy for chronic hepatitis B, GUT, vol 54, issue 1, BMJ Publishing Group, London England, pp. 152-161.

Tsai, S., Lee, T., Chien, R., Liao, S., Lin, C., Kuo, G.C., Liaw, Y., 2004, A method to increase tetramer staining efficiency of CD8+ T cells with MHC-peptide complexes: therapeutic applications in monitoring cytotoxic T lymphocyte activity during hepatitis B and C treatment, Journal of Immunological Methods, vol 285, issue 1, Elsevier Science BV, Amsterdam The Netherlands, pp. 71-87.

Mozsolits, H., Lee, T., Clayton, A.H.A., Sawyer, W.H., Aguilar, M., 2004, The membrane-binding properties of a class A amphipathic peptide, European Biophysics Journal with Biophysics Letters, vol 33, issue 2, Springer-Verlag, New York USA, pp. 98-108.

Mozsolits, H., Lee, T., Clayton, A.H.A., Sawyer, W.H., Aguilar, M., 2003, The membrane-binding properites of a class A amphipathic peptide, European Biophysics Journal with Biophysics Letters, vol 33, issue 2, Springer-Verlag, New York USA, pp. 98-108.

Lin, C., Lo, W., Lee, T., Ren, Y., Hwang, S., Cheng, Y., Chen, C., Chang, Y., Lee, S.P., Rickinson, A.B., Tam, P.K., 2002, Immunization with Epstein-Barr Virus (EBV) peptide-pulsed dendritic cells induces functional CD8+ T-cell immunity and may lead to tumor regression in patients with EBV-positive nasopharyngeal carcinoma, Cancer Research, vol 62, issue 23, American Association of Cancer Research, USA, pp. 6952-6958.

Lee, T., Mozsolits, H., Aguilar, M., 2001, Measurement of the affinity of melittin for switterionic and anionic membranes using immobilized lipid biosensors, Journal of Peptide Research, vol 58, Munksgaard Int Publ Ltd, Copenhagen Denmark, pp. 464-476.

Lee, T., Rivett, D.E., Werkmeister, J., Hewish, D., Aguilar, M., 1999, Interaction of amphipathic peptides with an immobilised model membrane, Letters in Peptide Science, vol 6, Kluwer/Escom, Dordrecht Nethrelands.

Mozsolits, H., Lee, T., Wirth, H.-., Perlmutter, P.P., Aguilar, M., 1999, The Interaction of Bioactive Peptides with an Immobilized Phosphatidylcholine Monolayer, Biophysical Journal, vol 77, Elsevier Science, Amsterdam Netherlands, pp. 1428-1444.

Mozsolits, H., Lee, T., Wirth, H.-., Perlmutter, P.P., Aguilar, M., 1999, The Interaction of Bioactive Peptides with an Immobilized Phosphatidylcholine Monolayer, Biophysical Journal, vol 77, Biophysical Society, Bethesda USA, pp. 1428-1444.

Lee, T., Thompson, P.E., Hearn, M.T., Aguilar, M., 1997, Conformational stability of a type IIb- turn motif in human growth hormone [6 - 13] peptide analogues at hydrophobic surfaces, Journal of Peptide Research, vol 49, Munksgaard International Publishers Ltd., Copenhagen Denmark, pp. 394-403.

Conference Proceedings

Lee, T., Mozsolits, H., Wirth, H.-., Aguilar, M., 1998, Immobilised lipid monolayers to study membrane-mediated peptide function, 3rd Australian Peptide Conference "From Discovery to Therapeutics", Laguna Quays The Whitsundays QLD Oct 4-9, 1998, Pink Panther Printing, Melbourne Vic Australia, p. 62.

Lee, T., Rivett, D.E., Clayton, A.H., Sawyer, W.H., Aguilar, M., 1998, Interaction of amphipathic peptides with an immobilised phospholipid monolayer, 3rd Australian Peptide Conference "From Discovery to Therapeutics", Laguna Quays The Whitsundays QLD Oct 4-9, 1998, Pink Panther Printing, Melbourne Vic Australia, p. 64.

Lee, T., Wirth, H.-., Perlmutter, P.P., Rivett, D.E., Aguilar, M., 1998, Interaction of melittin and truncated analogue with a phosphatidylcholine monolayer Immobilised slica support, Proceedings 23rd Lorne Annual Conference on Protein Structure and Function, Lorne VIC 8-12 February, 1998, Lorne Protein Workshop Inc, Melbourne Vic Australia, p. A8.

Lee, T., Wirth, H.-., Perlmutter, P.P., Rivett, D.E., Aguilar, M., 1998, The interaction of antimicrobial peptides with immobilised model membranes, Journal of Peptide Science Special Issue, Budapest Hungary 30 Aug - 4 Sept., 1998, European Peptide Symposium, Budapest Hungary, p. 394.

Lee, T., Wirth, H.-., Perlmutter, P.P., Aguilar, M., 1997, Dynamic behaviour of melittin in a phosphatidylcholine monolayer immobilised solid support, Australian Society for Biochemistry and Molecular Biology Incorporated and Australian Society of Plant Physiologists Incorporated, Melbourne, 29/9-2/10/97, ASBMB, Australia, p. B1-86.

Lee, T., Wirth, H.-., Perlmutter, P.P., Aguilar, M., 1997, Dynamic behaviour of melittin with a phospholipid monolayer immobilised silica support, Proceedings of the Australian Society for Biochemistry and Molecular Biology, Melbourne, Sept. 29-Oct. 2, 1997, Australian Soc. for Biochemistry & Molecular Biol., Kent Town SA, p. B1-86.

Wirth, H.-., Lee, T., Perlmutter, P.P., Aguilar, M., 1997, Immobilisation of model phospholipid membranes onto silica supports, Proceedings , 21st International Symposium on High Performance Liquid Chromatography, Birmingham, England, June 22-27, 1997, HPLC 97, Bradford UK, p. 1.

Lee, T., Wirth, H.-., Perlmutter, P.P., Aguilar, M., 1997, Interaction of melittin with phospholipid monolayer modified silica support, The 1997 American Peptide Symposium, Nashville, Tennessee, 14-19/6/97, APS, USA, p. P268.

Lee, T., Wirth, H.-., Perlmutter, P.P., Aguilar, M., 1997, Interaction of melittin with phospholipid monolayer modified silica support, Proceedings, 15th American Peptide Symposium, Nashville, TN, USA, June 14-19, 1997, American Peptide Society, Nashville TN USA, p. 2-136.

Other

Aguilar, M.I., Lee, T., Catimel, B., Nice, E., 2009, Miniaturization of high performance liquid chromatography separations and equipment in peptide and protein analysis, Encyclopedia of Analytical Chemistry, vol encyclopedia, John Wiley & Sons, Ltd, USA, pp. 1-21.

Lee, T., Perlmutter, P., Aguilar, M., 2003, Purification methods, Australia.

Lee, T., 1999, Design and synthesis of immobilised phospholipid monolayers for the study of peptide-biomembrane interactions.

Postgraduate Research Supervisions

Current Supervision

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

The mechanism of membrane disruption by antimicrobial peptides.

Supervisors:

Aguilar, M (Main), Lee, T (Associate).

Completed Supervision

Student:

Zhai, J.

Program of Study:

Protein adsorption at oil-water interfaces: interfacial structure and function of proteins in emulsions. (PHD) 2011.

Supervisors:

Aguilar, M (Main), Lee, T (Associate).