Dr Bronwyn Evans - Researcher Profile

Biography

Position

Senior Research Fellow
Faculty of Pharmacy and Pharmaceutical Sciences

Drug Discovery Biology
Monash Institute of Pharmaceutical Sciences

Research & Supervision Interests

• Biased signalling displayed by alpha and beta-adrenoceptors
• Adrenoceptor-mediated activation of cell growth and survival pathways
• Receptor interactions with scaffolding proteins and membrane rafts
• Contribution of molecular biology and bioinformatics to pharmacology research, including knockout mice, PCR arrays, high-throughput assays, receptor evolution

Qualifications

SCIENCE

Institution: Australian National University

Year awarded: 1982

Publications

Journal Articles

Sato, M., Hutchinson, D.S., Halls, M.L., Furness, S.G.B., Bengtsson, T., Evans, B.A., Summers, R.J., 2012, Interaction with caveolin-1 modulates G protein coupling of the mouse beta3-adrenoceptor, Journal Of Biological Chemistry [P], vol 287, issue 24, American Society for Biochemistry and Molecular Biology Inc., USA, pp. 20674-20688.

Dehvari, N., Hutchinson, D., Nevzorova, J., Dallner, O., Sato, M., Kocan, M., Merlin, J., Evans, B., Summers, R., Bengtsson, T., 2011, Beta(2)-adrenoceptors increase translocation of GLUT4 via GPCR kinase sites in the receptor C-terminal tail, British Journal of Pharmacology [P], vol 165, issue 5, John Wiley & Sons Ltd, UK, pp. 1442-1456.

Evans, B.A., Broxton, N., Merlin, J., Sato, M., Hutchinson, D.S., Christopoulos, A., Summers, R.J., 2011, Quantification of functional selectivity at the human {alpha}1A-adrenoceptor, Molecular Pharmacology [P], vol 79, issue 2, American Society for Pharmacology & Experimental Therapeutics, USA, pp. 298-307.

Evans, B., Sato, M., Sarwar, M., Hutchinson, D., Summers, R., 2010, Ligand-directed signalling at beta-adrenoceptors, British Journal of Pharmacology [P], vol 159, issue 5, John Wiley & Sons Ltd., United Kingdom, pp. 1022-1038.

Merlin, J., Evans, B.A., Csikasz, R.I., Bengtsson, T., Summers, R.J., Hutchinson, D.S., 2010, The M3-muscarinic acetylcholine receptor stimulates glucose uptake in L6 skeletal muscle cells by a CaMKK-AMPK-dependent mechanism, Cellular Signalling [P], vol 22, issue 7, Elsevier Inc., USA, pp. 1104-1113.

Halls, M.L., Papaioannou, M., Wade, J.D., Evans, B.A., Bathgate, R.A.D., Summers, R.J., 2009, RXFP1 couples to the Galphai3-Gbetagamma-PI3K-PKCzeta pathway via the final 10 amino acids of the receptor C-terminal tail, Annals of the New York Academy of Sciences [P], vol 1160, Wiley-Blackwell Publishing, Inc., USA, pp. 117-120.

Halls, M.L., van der Westhuizen, E.T., Wade, J.D., Evans, B.A., Bathgate, R.A.D., Summers, R.J., 2009, Relaxin family peptide receptor (RXFP1) coupling to Galphai3, involves the C-terminal Arg752 and localization within membrane raft microdomains, Molecular Pharmacology [P], vol 75, issue 2, American Society for Pharmacology and Experimental Therapeutics, USA, pp. 415-428.

Sato, M., Hutchinson, D.S., Evans, B.A., Summers, R.J., 2008, The beta3-adrenoceptor agonist 4-[[(hexylamino)carbonyl]amino]-N-[4-[2-[[(2S)-2-hydroxy-3-(4-hydroxyphenoxy)propyl]amino]ethyl]-phenyl]-benzenesulfonamide (L755507) and antagonist [...], Molecular Pharmacology, vol 74, issue 5, American Society for Pharmacology and Experimental Therapeutics, United States, pp. 1417-1428.

Sato, M., Hutchinson, D.S., Evans, B.A., Summers, R.J., 2007, Functional domains of the mouse beta3-adrenoceptor associated with differential G-protein coupling, Biochemical Society Transactions, vol 35, issue Pt 5, Portland Press Ltd, London, England, UK, pp. 1035-1037.

Sato, M., Horinouchi, T., Hutchinson, D.S., Evans, B.A., Summers, R.J., 2007, Ligand-directed signaling at the beta3-adrenoceptor produced by 3-(2-ethylphenoxy)-1-[(1,S)-1,2,3,4-tetrahydronapth-1-ylamino]-2S-2-propanol oxalate (SR59230A) relative to receptor agonists, Molecular Pharmacology, vol 72, issue 5, American Society of Pharmacology & Experimental Therapeutics, Bethesda MD USA, pp. 1359-1368.

Nevzorova, J., Evans, B.A., Bengtsson, T., Summers, R.J., 2006, Multiple signalling pathways involved in beta2-adrenoceptor-mediated glucose uptake in rat skeletal muscle cells, British Journal of Pharmacology, vol 147, issue 4, Nature Publishing Group, London UK, pp. 446-454.

Hutchinson, D.S., Sato, M., Evans, B.A., Christopoulos, A., Summers, R.J., 2005, Evidence for pleiotropic signaling at the mouse beta3-adrenoceptor revealed by SR59230A [3-(2-ethylphenoxy)-1-[(1,S)-1,2,3,4-tetrahydronapth-1-ylamino]-2S-2-propanol oxalate], The Journal of Pharmacology and Experimental Therapeutics, vol 312, issue 3, American Society for Pharmacology and Experimental Therapeutics, Bethesda USA, pp. 1064-1074.

Sato, M., Hutchinson, D.S., Bengtsson, T., Floren, A., Langel, U., Horinouchi, T., Evans, B.A., Summers, R.J., 2005, Functional domains of the mouse beta3-adrenoceptor associated with differential G-protein coupling, The Journal of Pharmacology and Experimental Therapeutics, vol 315, issue 3, American Society for Pharmacology and Experimental Therapeutics, USA, pp. 1354-1361.

Popp, B.D., Hutchinson, D.S., Evans, B.A., Summers, R.J., 2004, Stereoselectivity for interactions of agonists and antagonists at mouse, rat and human 3-adrenoceptors, European Journal of Pharmacology, vol 484, issue 2-3, Elsevier, Amsterdam The Netherlands, pp. 323-331.

Summers, R.J., Broxton, N., Hutchinson, D.S., Evans, B.A., 2004, The Janus faces of adrenoceptors: factors controlling the coupling of adrenoceptors to multiple signal transduction pathways, Clinical and Experimental Pharmacology and Physiology, vol 31, issue 11, Blackwell Publishing, Carlton Vic Australia, pp. 822-827.

Nevzorova, J., Bengtsson, T., Evans, B.A., Summers, R.J., 2002, Characterization of the -adrenoceptor subtype involved in mediation of glucose transport in L6 cells, British Journal of Pharmacology, vol 137, Nature Publishing Group, London England, pp. 9-18.

Hutchinson, D.S., Bengtsson, T., Evans, B.A., Summers, R.J., 2002, Mouse 3a- and 3b- adrenoceptors expressed in Chinese hamster ovary cells display identical pharmacology but utilize distinct signalling pathways, British Journal of Pharmacology, vol 135, Nature Publishing Group, London England, pp. 1903-1914.

Hutchinson, D.S., Evans, B.A., Summers, R.J., 2001, b1-Adrenoceptors compensate for b3-adrenoceptors in ileum from b3-adrenoceptor knock-out mice, British Journal of Pharmacology, vol 132, issue 2, Nature Publishing Group, Basingstoke England, pp. 433-442.

Hutchinson, D.S., Evans, B.A., Summers, R.J., 2000, Beta3-adrenoceptor regulation and relaxation responses in mouse ileum, British Journal of Pharmacology, vol 129, Stockton Press, Basingstoke England, pp. 1251-1259.

Evans, B.A., Papaioannou, M., Hamilton, S., Summers, R.J., 1999, Alternative splicing generates two isoforms of the beta3-adrenoceptor which are differentially expressed in mouse tissues, British Journal of Pharmacology, vol 127, Stockton Press, Basingstoke England, pp. 1525-1531.

Roberts, S.J., Papaioannou, M., Evans, B.A., Summers, R.J., 1999, Characterization of beta-adrenoceptor mediated smooth muscle relaxation and the detection of mRNA for beta1- beta2- and beta3- adrenoceptors in rat ileum, British Journal of Pharmacology, vol 127, Stockton Press, Basingstoke England, pp. 949-961.

Kompa, A.R., Gu, X., Evans, B.A., Summers, R.J., 1999, Desensitisation of cardiac beta-adrenoceptor signaling with heart failure produced by myocardial infarction in the rat. Evidence for the role of Gi but not Gs or phosphorylating proteins, Journal of Molecular and Cellular Cardiology, vol 31, Academic Press, London England, pp. 1185-1201.

Evans, B.A., Agar, L., Summers, R.J., 1999, The role of the sympathetic nervous system in the regulation of leptin synthesis in C57BL/6 mice, Federation of European Biochemical Societies Letters, vol 444, Elsevier, Amsterdam Netherlands, pp. 149-154.

Conference Proceedings

Sato, M., Horinouchi, T., Hutchinson, D.S., Evans, B.A., 2006, Multiple signalling pathways of the mouse beta(3)-adrenoceptor stably expressed in CHO-K1 cells, Acta Pharmacologica Sinica, 2-7/7/2006, Blackwell Publishing, Oxford London UK, p. 410.

Horinouchi, T., Sato, M., Hutchinson, D.S., Evans, B.A., Summers, R.J., 2006, The role of the C-terminal domain in mouse beta(3)-adrenoceptor signalling, Journal of Pharmacological Sciences, 26-30/6/2006, Japanese Pharmacogical Society, Kyoto Japan, p. 126.

Nevzorova, J., Evans, B.A., Summers, R.J., 2006, The role of the C-terminal tail of the human beta(2)-adrenoceptor in stimulation of glucose uptake in CHO cells, Acta Pharmacologica Sinica, 2-7/7/2006, Blackwell Publishing, Oxford England, p. 394.

Evans, B.A., Summers, R.J., 1999, The search for a fourth beta-adrenoceptor, Proceedings of the Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists, Sydney, NSW, Dec 1999, ASCEPT, Sydney NSW Australia, pp. 27-Oral 2-6.

Summers, R.J., Papaioannou, M., Hamilton, S., Evans, B.A., 1998, Alternative splicing generates two isoforms of the b3 - adrenoceptor which are differentially expressed in mouse tissues, British Pharmacology Society Meeting, Harrogate, UK, December, Stockton Press, Basingstoke Hampshire UK, p. 52P.

Summers, R.J., Papaioannou, M., Hamilton, S., Evans, B.A., 1998, Alternative splicing generates two isoforms of the mouse b-adrenoceptor, Pharmacology and Toxicology, Munchen, Germany, Aug.1998, Munksgaard, Copenhagen, p. 94 A11.

Summers, R.J., Papaioannou, M., Hamilton, S., Evans, B.A., 1998, Alternative splicing generates two isoforms of the mouse b3 -adrenoceptor, Naunyn-Schmiedeberg's Archives of Pharmacology, Munchen, Germany, July 1998, Springer, Berlin, p. R612.

Summers, R.J., Hutchinson, D.S., Evans, B.A., 1998, B3 -adrenorecoptors: differential regulations and novel subtypes, Pharmacology and Toxicology, Nordic Pharmacological Society, Copenhagan Denmark, pp. 37-39.

Evans, B.A., Hutchinson, D.S., Papaioannou, M., Hamilton, S., Summers, R.J., 1998, Cloing and expression of two isoforms of the b3 -adrenoceptor which are differentially expressed in mouse tissues, Proceedings of the Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists, Hobart, TAS, December 1998, ASCEPT, Sydney NSW Australia, p. 130.

Hutchinson, D.S., Evans, B.A., Summers, R.J., 1998, Differential regulation of b3-adrenoceptors (ARs) in mouse ileum and adipose tissues by the b3-AR agonist CL 316243 and the b3-AR antagonist SR 59230A, Naunyn-Schmiedeberg's Archives of Pharmacology, Munchen, Germany, July 1998, Springer, Berlin, p. R613.

Summers, R.J., Papaioannou, M., Hamilton, S., Evans, B.A., 1998, Generation of two isoforms of the mouse b3-adrenoceptor by alternative splicing, Proceedings of the Australian Neuroscience Society, Canberra, ACT, Jan 1998, Australian Neuroscience Society, Canberra ACT Australia, p. 150 P111.

Kompa, A.R., Evans, B.A., Summers, R.J., 1998, Reduced b-adrenoceptor(b-AR)function in a rat model of myocardial infarction(MI) is not associated with changes in expression of receptor phosphorylating enzymes, Proceedings of the Australian Neuroscience Society, Canberra, ACT, Jan 1998, Australian Neuroscience Society, Canberra ACT Australia, p. 145 P102.

Hutchinson, D.S., Evans, B.A., Summers, R.J., 1998, The pharmacological properties of the mouse b3a and b3b - adrenoceptor expressed in CHO - KI cells studied using the cytosensor R microphysiometer, Proceedings of the Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists, Hobart, TAS, December 1998, ASCEPT, Sydney NSW Australia, p. 174.

Teaching Commitment

• Applications of molecular pharmacology, including receptor expression, mutagenesis and characterisation, mRNA quantitation (PCR, in situ hybridisation, micro arrays), knockout and transgenic mice, siRNA
• Identification of endogenous ligands for novel G protein-coupled receptors

Activities

Affiliations with institutes

Drug Discovery Biology
Monash Institute of Pharmaceutical Sciences

Postgraduate Research Supervisions

Current Supervision

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

What makes the alpha1A-adrenoceptor gene express the alpha1L-adrenoceptor functional phenotype?.

Supervisors:

Evans, B (Joint), Ventura, S (Joint-co), May, L (Associate), Summers, R (Associate).

Completed Supervision

Student:

Nevzorova, Y.

Program of Study:

PHARMACOLOGICAL CHARACTERISATION AND SIGNALLING PATHWAYS OF G-PROTEIN COUPLED RECEPTOR-MEDIATED GLUCOSE TRANSPORT. (PHD) 2004.

Supervisors:

Summers, R (Joint-Co), Evans, B (Joint).

Student:

Phiri, N.

Program of Study:

MOLECULAR PHARMACOLOGY OF a1A-ADRENOCEPTOR ISOFORMS. (PHD) 2005.

Supervisors:

Evans, B (Joint), Summers, R (Joint-Co).

Student:

Popp, B.

Program of Study:

Investigation of the atypical pharmacology of the beta1-adrenoceptor. (PHD) 2009.

Supervisors:

Evans, B (Joint), Summers, R (Joint-Co).

Student:

Sato, M.

Program of Study:

Signal transduction pathways utilised by mouse and human B3-adrenoceptors. (PHD) 2007.

Supervisors:

Evans, B (Joint), Summers, R (Joint-Co).