Prof James Whisstock - Researcher Profile

Qualifications

PhD

Institution: Uni of Cambridge

Year awarded: 2005

Publications

Book Chapters

Dunstone, M.A., Whisstock, J.C., 2011, Crystallography of serpins and serpin complexes, in Methods in Enzymology, Volume 501: Serpin Structure and Evolution, eds James C Whisstock and Phillip I Bird, Academic Press, USA, pp. 63-87.

Song, J., Matthews, A., Reboul, C.F., Kaiserman, D., Pike, R.N., Bird, P.I., Whisstock, J.C., 2011, Predicting serpin/protease interactions, in Methods in Enzymology, Volume 501: Serpin Structure and Evolution, eds James C Whisstock and Philip I Bird, Academic Press, USA, pp. 237-273.

Whisstock, J.C., Bird, P.I., 2011, Preface, in Methods in Enzymology - Biology of Serpins, eds JN Abelson & MI Simon, Academic Press Elsevier Inc, UK, p. 19.

Irving, J.A., Cabrita, L., Kaiserman, D., Worrall, D.M., Whisstock, J., 2007, Evolution and classification of the serpin superfamily, in Molecular and Cellular Aspects of the Serpinopathies and Disorders in Serpin Activity, eds G.A. Silverman & D.A. Lomas, World Scientific, Singapore, pp. 1-33.

Grigoryev, S.A., Bulynko, Y.A., Pike, R.N., Whisstock, J., 2007, MENT a chromatin-associated serpin from avian blood cells: structure and function, in Molecular and Cellular Aspects of the Serpinopathies and Disorders in Serpin Activity, eds G.A. Silverman & D.A. Lomas, World Scientific, Singapore, pp. 243-259.

Zhang, Q., Law, R.H.P., Buckle, A.M., Cabrita, L., McGowan, S., Irving, J.A., Faux, N.G., Lesk, A.M., Bottomley, S.P., Whisstock, J., 2007, Serpins in Prokaryotes, in Molecular and Cellular Aspects of the Serpinopathies and Disorders in Serpin Activity, eds G.A. Silverman & D.A. Lomas, World Scientific, Singapore, pp. 131-162.

Journal Articles

Yan, X., Porter, C.J., Hardy, S.P., Steer, D.L., Smith, A.I., Quinsey, N.S., Hughes, V.A., Cheung, K.J., Keyburn, A., Kaldhusdal, M., Moore, R., Bannam, T.L., Whisstock, J., Rood, J.I., 2013, Structural and Functional Analysis of the Pore-Forming Toxin NetB from Clostridium perfringens, mBio [E], vol 4, issue 1, American Society for Microbiology, USA, pp. 1-9.

Langendorf, C.G., Tuck, K.L., Key, T., Fenalti, G., Pike, R.N., Rosado, C.J., Wong, A.S., Buckle, A.M., Law, R.H.P., Whisstock, J., 2013, Structural characterization of the mechanism through which human glutamic acid decarboxylase auto-activates, Bioscience Reports [P], vol 33, issue 1, Portland Press Ltd, UK, pp. 137-149.

Teoh, S.S., Wang, H., Risbridger, G.P., Whisstock, J.C., Bird, P.I., 2012, A versatile monoclonal antibody specific to human SERPINB5, Hybridoma [E], vol 31, issue 5, Mary Ann Liebert Inc. Publishers, USA, pp. 333-339.

Mahmood, K., Webb, G.I., Song, J., Whisstock, J.C., Konagurthu, A.S., 2012, Efficient large-scale protein sequence comparison and gene matching to identify orthologs and co-orthologs, Nucleic Acids Research [P], vol 40, issue 6 (Art. ID: e44), Oxford University Press, UK, pp. 1-11.

Poreba, M., McGowan, S., Skinner-Adams, T.S., Trenholme, K.R., Gardiner, D.L., Whisstock, J.C., To, J., Salvesen, G.S., Dalton, J.P., Drag, M., 2012, Fingerprinting the substrate specificity of M1 and M17 aminopeptidases of human malaria, Plasmodium falciparum, PLoS ONE [P], vol 7, issue 2 (Art. ID: e31938), Public Library of Science, USA, pp. 1-8.

Song, J., Tan, H., Perry, A.J., Akutsu, T., Webb, G.I., Whisstock, J.C., Pike, R.N., 2012, PROSPER: an integrated feature-based tool for predicting protease substrate cleavage sites, PLoS ONE [P], vol 7, issue 11 (Art. No.: e50300), Public Library of Science, USA, pp. 1-23.

D'Angelo, M., Dunstone, M.A., Whisstock, J.C., Trapani, J., Bird, P.I., 2012, Perforin evolved from a gene duplication of MPEG1, followed by a complex pattern of gene gain and loss within Euteleostomi, BMC Evolutionary Biology [P], vol 12, issue Art. ID: 59, BioMed Central Ltd, UK, pp. 1-12.

Reboul, C.F., Mahmood, K., Whisstock, J.C., Dunstone, M.A., 2012, Predicting giant transmembrane ß-barrel architecture, Bioinformatics [P], vol 28, issue 10, Oxford University Press, UK, pp. 1299-1302.

Lopez, J.A., Brennan, A.J., Whisstock, J.C., Voskoboinik, I., Trapani, J.A., 2012, Protecting a serial killer: pathways for perforin trafficking and self-defence ensure sequential target cell death, Trends In Immunology [P], vol 33, issue 8, Elsevier Ltd. * Trends Journals, UK, pp. 406-412.

Lo, C.Y.J., Jamsai, D., O'Connor, A.E., Borg, C.L., Clark, B., Whisstock, J.C., Field, M.C., Adams, V., Ishikawa, T., Aitken, R.J., Whittle, B., Goodnow, C.C., Ormandy, C.J., O'Bryan, M.K., 2012, RAB-like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly, PLoS Genetics [E], vol 8, issue 10 (Art. No.: e1002969), Public Library of Science, USA, pp. 1-11.

Orengo, C., Whisstock, J., 2012, Structural insights into multi-protein communication systems, Current Opinion in Structural Biology [P], vol 22, issue 3, Elsevier Ltd * Current Opinion Journals, UK, pp. 313-315.

Han, X.Y., Kennan, R.M., Steer, D.L., Smith, A.I., Whisstock, J.C., Rood, J.I., 2012, The AprV5 subtilase is required for the optimal processing of all three extracellular serine proteases from Dichelobacter nodosus, PLoS ONE [P], vol 7, issue 10 (Art. No.: e47932), Public Library of Science, USA, pp. 1-8.

Porter, C.J., Bantwal, R., Bannam, T.L., Rosado, C.J., Pearce, M.C., Adams, V.M., Lyras, D., Whisstock, J.C., Rood, J.I., 2012, The conjugation protein TcpC from Clostridium perfringens is structurally related to the type IV secretion system protein VirB8 from Gram-negative bacteria, Molecular Microbiology [P], vol 83, issue 2, Wiley-Blackwell Publishing Ltd, UK, pp. 275-288.

Linke, C., Siemens, N., Oehmcke, S., Radjainia, M., Law, R.H., Whisstock, J.C., Baker, E.N., Kreikemeyer, B., 2012, The extracellular protein factor Epf from Streptococcus pyogenes is a cell surface adhesin that binds to cells through an n-terminal domain containing a carbohydrate-binding module, Journal Of Biological Chemistry [P], vol 287, issue 45, American Society for Biochemistry and Molecular Biology, Inc., USA, pp. 38178-38189.

Law, R.H., Caradoc-Davies, T.T., Cowieson, N.P., Horvath, A.J., Quek, A.J., Amarante Da Encarnacao, J., Steer, D.L., Cowan, A.D., Zhang, Q., Lu, B.G., Pike, R.N., Smith, A.I., Coughlin, P.B., Whisstock, J.C., 2012, The x-ray crystal structure of full-length human plasminogen, Cell Reports [E], vol 1, issue 3, Cell Press, USA, pp. 185-190.

Sivaraman, K.K., Oellig, C.A., Huynh, K., Atkinson, S.C., Poreba, M., Perugini, M.A., Trenholme, K.R., Gardiner, D.L., Salvesen, G., Drag, M., Dalton, J.P., Whisstock, J.C., McGowan, S., 2012, X-ray crystal structure and specificity of the Plasmodium falciparum malaria aminopeptidase PfM18AAP, Journal of Molecular Biology [P], vol 422, issue 4, Academic Press, UK, pp. 495-507.

McGowan, S., Buckle, A.M., Mitchell, M.S., Hoopes, J.T., Gallagher, D.T., Heselpoth, R.D., Shen, Y., Reboul, C.F., Law, R.H., Fischetti, V.A., Whisstock, J.C., Nelson, D.C., 2012, X-ray crystal structure of the streptococcal specific phage lysin PlyC, Proceedings Of The National Academy Of Sciences Of The United States Of America [P], vol 109, issue 31, National Academy of Sciences, USA, pp. 12752-12757.

Stivala, A., Wybrow, M., Wirth, A., Whisstock, J., Stuckey, P., 2011, Automatic generation of protein structure cartoons with Pro-origami, Bioinformatics [P], vol 27, issue 23, Oxford University Press, Oxford England, pp. 3315-3316.

Harbut, M., Velmourougane, G., Dalal, S., Reiss, G., Whisstock, J., Onder, O., Brisson, D., McGowan, S., Klemba, M., Greenbaum, D., 2011, Bestatin-based chemical biology strategy reveals distinct roles for malaria M1- and M17-family aminopeptidases, Proceedings Of The National Academy Of Sciences Of The United States Of America [P], vol 108, issue 34, National Academy of Sciences, USA, pp. E526-E534.

Song, J., Tan, H., Boyd, S.E., Shen, H., Mahmood, K., Webb, G., Akutsu, T., Whisstock, J., Pike, R., 2011, Bioinformatic approaches for predicting substrates of proteases, Journal of Bioinformatics and Computational Biology [P], vol 9, issue 1, Imperial College Press, UK, pp. 149-178.

Alonzo III, F., Xayarath, B., Whisstock, J., Freitag, N., 2011, Functional analysis of the Listeria monocytogenes secretion chaperone PrsA2 and its multiple contributions to bacterial virulence, Molecular Microbiology [P], vol 80, issue 6, Wiley-Blackwell Publishing Ltd, UK, pp. 1530-1548.

Brennan, A., Chia, J., Browne, K., Ciccone, A., Ellis, S., Lopez, J., Susanto, O., Verschoor, S., Yagita, H., Whisstock, J., Trapani, J., Voskoboinik, I., 2011, Protection from endogenous perforin: Glycans and the C terminus regulate exocytic trafficking in cytotoxic lymphocytes, Immunity [P], vol 34, issue 6, Cell Press, USA, pp. 879-892.

Wong, W., Wijeyewickrema, L.C., Kennan, R.M., Reeve, S.B., Steer, D.L., Reboul, C., Smith, A.I., Pike, R.N., Rood, J.I., Whisstock, J.C., Porter, C.J., 2011, S1 pocket of a bacterially derived subtilisin-like protease underpins effective tissue destruction, Journal Of Biological Chemistry [P], vol 286, issue 49, American Society for Biochemistry and Molecular Biology, Inc., USA, pp. 42180-42187.

Velmourougane, G., Harbut, M.B., Dalal, S., McGowan, S., Oellig, C., Meinhardt, N., Whisstock, J.C., Klemba, M., Greenbaum, D.C., 2011, Synthesis of new (-)-bestatin-based inhibitor libraries reveals a novel binding mode in the S1 pocket of the essential malaria M1 metalloaminopeptidase, Journal of Medicinal Chemistry [P], vol 54, issue 6, American Chemical Society, USA, pp. 1655-1666.

Song, J., Tan, H., Shen, H., Mahmood, K., Boyd, S.E., Webb, G., Akutsu, T., Whisstock, J., 2010, Cascleave: towards more accurate prediction of caspase substrate cleavage sites, Bioinformatics [P], vol 26, issue 6, Oxford University Press, UK & USA, pp. 752-760.

Wong, W., Kennan, R.M., Rosado, C.J., Rood, J.I., Whisstock, J., Porter, C.J., 2010, Crystallization of the virulent and benign subtilisin-like proteases from the ovine footrot pathogen Dichelobacter nodosus, Acta Crystallographica Section F: Structural Biology..., vol 66, issue 3, Wiley-Blackwell Publishing, Inc., US, UK, pp. 289-293.

Mahmood, K., Konagurthu, A.S., Song, J., Buckle, A.M., Webb, G.I., Whisstock, J.C., 2010, EGM: Encapsulated gene-by-gene matching to identify gene orthologs and homologous segments in genomes, Bioinformatics [P], vol 26, issue 17, Oxford University Press, UK, USA, pp. 2076-2084.

Pike, R.N., Whisstock, J., 2010, Highlight: The Biology of Proteolytic Systems, Biological Chemistry [P], vol 391, issue 8, Walter de Gruyter GmbH & Co KG, Germany, p. 837.

Konagurthu, A.S., Reboul, C.F., Schmidberger, J.W., Irving, J.A., Lesk, A.M., Stuckey, P.J., Whisstock, J.C., Buckle, A.M., 2010, MUSTANG-MR structural sieving server: Applications in protein structural analysis and crystallography, PLoS ONE [P], vol 5, issue 4 (e10048), Public Library of Science, USA, pp. 1-7.

Teoh, S., Whisstock, J., Bird, P.I., 2010, Maspin (SERPINB5) is an obligate intracellular serpin, Journal Of Biological Chemistry [P], vol 285, issue 14, American Society for Biochemistry and Molecular Biology, Inc., USA, pp. 10862-10869.

Huntington, J.A., Whisstock, J., 2010, Molecular contortionism - on the physical limits of serpin 'loop-sheet' polymers, Biological Chemistry [P], vol 391, issue 8, Walter de Gruyter GmbH & Co KG, Germany, pp. 973-982.

Schmidberger, J.W., Bate, M., Reboul, C.F., Androulakis, S.G., Phan, J.M.N., Whisstock, J., Goscinski, W., Abramson, D.A., Buckle, A.M., 2010, Mrgrid: A portable grid based molecular replacement pipeline, PLoS ONE [P], vol 5, issue 4 (e10049), Public Library of Science, USA, pp. 1-6.

Voskoboinik, I., Dunstone, M.A., Baran, K., Whisstock, J., Trapani, J.A., 2010, Perforin: structure, function, and role in human immunopathology, Immunological Reviews [P], vol 235, issue 1, Wiley-Blackwell Publishing Inc, USA, pp. 35-54.

Skinner-Adams, T.S., Stack, C.M., Trenholme, K.R., Brown, C.L., Grembecka, J., Lowther, J., Mucha, A., Drag, M., Kafarski, P., McGowan, S., Whisstock, J., Gardiner, D.L., Dalton, J.P., 2010, Plasmodium falciparum neutral aminopeptidases: new targets for anti-malarials, Trends In Biochemical Sciences [P], vol 35, Elsevier Ltd, UK & Switzerland, pp. 53-61.

Silverman, G.A., Whisstock, J.C., Bottomley, S.P., Huntington, J.A., Kaiserman, D., Luke, C.J., Pak, S.C., Reichhart, J.-., Bird, P.I., 2010, Serpins flex their muscle: I. Putting the clamps on proteolysis in diverse biological systems, Journal Of Biological Chemistry [P], vol 285, issue 32, American Society for Biochemistry and Molecular Biology Inc., USA, pp. 24299-24305.

Whisstock, J.C., Silverman, G.A., Bird, P.I., Bottomley, S.P., Kaiserman, D., Luke, C.J., Pak, S.C., Reichhart, J.-., Huntington, J.A., 2010, Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functions, Journal Of Biological Chemistry [P], vol 285, issue 32, American Society for Biochemistry and Molecular Biology Inc., USA, pp. 24307-24312.

McGowan, S., Oellig, C., Birru, W.A., Caradoc-Davies, T.T., Stack, C.M., Lowther, J., Skinner-Adams, T.S., Mucha, A., Kafarski, P., Grembecka, J., Trenholme, K.R., Buckle, A.M., Gardiner, D.L., Dalton, J.P., Whisstock, J., 2010, Structure of the Plasmodium falciparum M17 aminopeptidase and significance for the design of drugs targeting the neutral exopeptidases, Proceedings Of The National Academy Of Sciences Of The United States Of America [P], vol 107, issue 6, National Academy of Sciences, USA, pp. 2449-2454.

Langendorf, C., Key, T., Fenalti, G., Kan, W., Buckle, A.M., Caradoc-Davies, T.T., Tuck, K.L., Law, R.H.P., Whisstock, J., 2010, The X-ray crystal structure of Escherichia coli succinic semialdehyde dehydrogenase; structural insights into NADP+/enzyme interactions, PLoS ONE [P], vol 5, issue 2, Pubic Library of Science, USA, pp. 1-12.

Law, R.H.P., Lukoyanova, N., Voskoboinik, I., Caradoc-Davies, T.T., Baran, K., Dunstone, M.A., D'Angelo, M., Orlova, E.V., Coulibaly, F.J., Verschoor, S., Browne, K.A., Ciccone, A., Kuiper, M.J., Bird, P.I., Trapani, J.A., Saibil, H.R., Whisstock, J.C., 2010, The structural basis for membrane binding and pore formation by lymphocyte perforin, Nature [P], vol 468, issue 7322, Nature Publishing Group, UK, pp. 447-451.

Kondos, S., Hatfaludi, T.Z., Voskoboinik, I., Trapani, J.A., Law, R.H., Whisstock, J.C., Dunstone, M.A., 2010, The structure and function of mammalian membrane-attack complex/perforin-like proteins, Tissue Antigens [P], vol 76, issue 5, Wiley-Blackwell Publishing Inc, USA, pp. 341-351.

Kennan, R.M., Wong, W., Dhungyel, O., Han, X., Wong, D.M., Parker, D., Rosado, C.J., Law, R.H., McGowan, S., Reeve, S.B., Levina, V., Powers, G.A., Pike, R.N., Bottomley, S.P., Smith, A.I., Marsh, I., Whittington, R.J., Whisstock, J., Porter, C.J., Rood, J.I., 2010, The subtilisin-like protease AprV2 is required for virulence and uses a novel disulphide-tethered exosite to bind substrates, Plos Pathogens [P], vol 6, issue 11, Public Library of Science, USA, pp. 1-12.

Newton, H.J., Pearson, J.S., Badea, L., Kelly, M., Lucas, M., Holloway, G., Wagstaff, K.M., Dunstone, M.A., Sloan, J., Whisstock, J.C., Kaper, J.B., Robins-Browne, R.M., Jans, D.A., Frankel, G., Phillips, A.D., Coulson, B.S., Hartland, E.L., 2010, The type III effectors NleE and NleB from enteropathogenic E. coli and OspZ from Shigella block nuclear translocation of NF-kappaB p65, Plos Pathogens [P], vol 6, issue 5 (e1000898), Public Library of Science, USA, pp. 1-16.

Ong, P.C., Golding, S.J., Pearce, M.C., Irving, J.A., Grigoryev, S.A., Pike, D., Langendorf, C.G., Bashtannyk-Puhalovich, T.A., Bottomley, S.P., Whisstock, J.C., Pike, R.N., McGowan, S., 2009, Conformational change in the chromatin remodelling protein MENT, PL o S One, vol 4, issue 3 (e4727), Public Library of Science, USA, pp. 1-5.

Murray-Rust, T.A., Kerr, F.K., Thomas, A.R., Wu, T.J., Yongqing, T., Ong, P.C., Quinsey, N.S., Whisstock, J.C., Wagenaar-Bos, I.C., Freeman, C., Pike, R.N., 2009, Modulation of the proteolytic activity of the complement protease C1s by polyanions: Implications for polyanion-mediated acceleration of interaction between C1s and SERPING1, Biochemical Journal [P], vol 422, issue 2, Portland Press Ltd, UK, pp. 295-303.

Song, J., Tan, H., Mahmood, K., Law, R.H.P., Buckle, A.M., Webb, G., Akutsu, T., Whisstock, J., 2009, Prodepth: Predict residue depth by support vector regression approach from protein sequences only, PLoS ONE [P], vol 4, issue 9 (e7072), Public Library of Science, USA, pp. 1-14.

McGowan, S., Porter, C.J., Lowther, J., Stack, C.M., Golding, S.J., Skinner-Adams, T.S., Trenholme, K.R., Teuscher, F., Donnelly, S.M., Grembecka, J., Mucha, A., Kafarski, P., DeGori, R., Buckle, A.M., Gardiner, D.L., Whisstock, J.C., Dalton, J.P., 2009, Structural basis for the inhibition of the essential Plasmodium falciparum M1 neutral aminopeptidase, Proceedings of the National Academy of Sciences of the United States of America, vol 106, issue 8, National Academy of Sciences, USA, pp. 2537-2542.

Arafat, Y., Fenalti, G., Whisstock, J., Mackay, I.R., Garcia De La Banda, M.J., Rowley, M.J., Buckle, A.M., 2009, Structural determinants of GAD antigenicity, Molecular Immunology [P], vol 47, issue 2-3, Pergamon, UK, pp. 493-505.

Fischer, K., Langendorf, C., Irving, J.A., Reynolds, S., Willis, C., Beckham, S.A., Law, R.H.P., Yang, S., Bashtannyk-Puhalovich, T.A., McGowan, S., Whisstock, J., Pike, R.N., Kemp, D.J., Buckle, A.M., 2009, Structural mechanisms of inactivation in scabies mite serine protease paralogues, Journal of Molecular Biology [P], vol 390, issue 4, Academic Press, UK, pp. 635-645.

Kaiserman, D., Buckle, A.M., Van Damme, P., Irving, J.A., Law, R.H.P., Matthews, A.Y., Bashtannyk-Puhalovich, T.A., Langendorf, C., Thompson, P., Vandekerckhove, J., Gevaert, K., Whisstock, J.C., Bird, P.I., 2009, Structure of granzyme C reveals an unusual mechanism of protease autoinhibition, Proceedings of the National Academy of Sciences of the United States of America, vol 106, issue 14, National Academy of Sciences, USA, pp. 5587-5592.

Chia, J., Yeo, K., Whisstock, J., Dunstone, M.A., Trapani, J.A., Voskoboinik, I., 2009, Temperature sensitivity of human perforin mutants unmasks subtotal loss of cytotoxicity, delayed FHL, and a predisposition to cancer, Proceedings Of The National Academy Of Sciences O..., vol 106, issue 24, National Academy of Sciences, USA, pp. 9809-9814.

Baran, K., Dunstone, M.A., Chia, J., Ciccone, A., Browne, K.A., Clarke, C.J.P., Lukoyanova, N., Saibil, H., Whisstock, J., Voskoboinik, I., Trapani, J.A., 2009, The molecular basis for perforin oligomerization and transmembrane pore assembly, Immunity [P], vol 30, issue 5, Cell Press, USA, pp. 684-695.

Steenbakkers, P.J., Irving, J.A., Harhangi, H.R., Swinkels, W.J., Akhmanova, A., Dijkerman, R., Jetten, M.S., Van Der Drift, C., Whisstock, J., Op Den Camp, H.J., 2008, A serpin in the cellulosome of the anaerobic fungus Piromyces sp. strain E2, Mycological Research, vol 112, issue 8, Elsevier Ltd., UK, pp. 999-1006.

Zhang, Q., Law, R.H.P., Bottomley, S.P., Whisstock, J., Buckle, A.M., 2008, A structural basis for Loop C-sheet polymerization in serpins, Journal of Molecular Biology, vol 376, issue 5, Academic Press, United Kingdom, pp. 1348-1359.

Fenalti, G., Hampe, C.S., Arafat, Y., Law, R.H.P., Banga, J.P., Mackay, I.R., Whisstock, J., Buckle, A.M., Rowley, M.J., 2008, COOH-terminal clustering of autoantibody and T-cell determinants on the structure of GAD65 provide insights into the molecular basis of autoreactivity, Diabetes, vol 57, issue 5, American Diabetes Association, United States, pp. 1293-1301.

Kerr, F.K., Thomas, A.R., Wijeyewickrema, L.C., Whisstock, J., Boyd, S.E., Kaiserman, D., Matthews, A.Y., Bird, P.I., Thielens, N.M., Rossi, V., Pike, R.N., 2008, Elucidation of the substrate specificity of the MASP-2 protease of the lectin complement pathway and identification of the enzyme as a major physiological target of the serpin, C1-inhibitor, Molecular Immunology, vol 45, Pergamon-Elsevier Science Ltd, Oxford England UK, pp. 670-677.

Androulakis, S.G., Schmidberger, J., Bate, M.A., DeGori, R., Beitz, A., Keong, C., Cameron, B., McGowan, S., Porter, C.J., Harrison, A., Hunter, J., Martin, J.L., Kobe, B., Dobson, R.C., Parker, M.W., Whisstock, J., Gray, J., Treloar, A.E., Groenewegen, D., Dickson, N., Buckle, A.M., 2008, Federated repositories of X-ray diffraction images, Acta Crystallographica. Section D: Biological Crystallography, vol 64, issue 7, Wiley-Blackwell Munksgaard, Denmark, pp. 810-814.

Whisstock, J., Bottomley, S.P., 2008, Structural biology: Serpins' mystery solved, Nature, vol 455, issue 7217, Nature Publishing Group, United Kingdom, pp. 1189-1190.

Rosado, C.J., Kondos, S., Bull, T.E., Kuiper, M., Law, R.H.P., Buckle, A.M., Voskoboinik, I., Bird, P.I., Trapani, J.A., Whisstock, J., Dunstone, M.A., 2008, The MACPF/CDC family of pore-forming toxins, Cellular Microbiology, vol 10, issue 9, Wiley-Blackwell Publishing Ltd., United Kingdom, pp. 1765-1774.

Schmidberger, J.W., Wilce, J.A., Weightman, A.J., Whisstock, J., Wilce, M.C.J., 2008, The crystal structure of DehI reveals a new alpha-haloacid dehalogenase fold and active-site mechanism, Journal of Molecular Biology, vol 378, issue 1, Academic Press, UK, pp. 284-294.

Law, R.H.P., Sofian, T., Kan, W., Horvath, A.J., Hitchen, C.R., Langendorf, C., Buckle, A.M., Whisstock, J., Coughlin, P.B., 2008, X-ray crystal structure of the fibrinolysis inhibitor alpha2-antiplasmin, Blood, vol 111, American Society of Hematology, Washington, DC, USA, pp. 2049-2052.

Rosado, C.J., Buckle, A.M., Law, R.H.P., Butcher, R.E., Kan, W., Bird, C.H., Ung, K., Browne, K.A., Baran, K., Bashtannyk-Puhalovich, T.A., Faux, N.G., Wong, W., Porter, C.J., Pike, R.N., Ellisdon, A.M., Pearce, M.C., Bottomley, S.P., Emsley, J., Smith, A.I., Rossjohn, J., Hartland, E.L., Voskoboinik, I., Trapani, J.A., Bird, P.I., Dunstone, M.A., Whisstock, J., 2007, A common fold mediates vertebrate defense and bacterial attack, Science, vol 317, issue 5844, American Association of Advancement in Science, Washington, DC, USA, pp. 1548-1551.

Cabrita, L., Irving, J.A., Pearce, M.C., Whisstock, J., Bottomley, S.P., 2007, Aeropin from the extremophile pyrobaculum aerophilum bypasses the serpin misfolding trap, Journal of Biological Chemistry, vol 282, issue 37, American Society of Biochemistry Molecular Biology Inc, Bethesda MD USA, pp. 26802-26809.

Ong, P.C., McGowan, S., Pearce, M.C., Irving, J.A., Kan, W., Grigoryev, S.A., Turk, B., Silverman, G.A., Brix, K., Bottomley, S.P., Whisstock, J., Pike, R.N., 2007, DNA accelerates the inhibition of human cathepsin V by serpins, Journal of Biological Chemistry, vol 282, issue 51, American Society of Biochemistry & Molecular Biology Inc, Bethesda MD USA, pp. 36980-36986.

Fenalti, G., Law, R.H.P., Buckle, A.M., Langendorf, C., Tuck, K.L., Rosado, C.J., Faux, N.G., Mahmood, K., Hampe, C.S., Banga, J.P., Wilce, M.C.J., Schmidberger, J., Rossjohn, J., El-Kabbani, O., Pike, R.N., Smith, A.I., Mackay, I.R., Rowley, M.J., Whisstock, J., 2007, GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop, Nature Structural & Molecular Biology, vol 14, issue 4, Nature Publishing Group, New York NY USA, pp. 280-286.

Faux, N.G., Huttley, G.A., Mahmood, K., Webb, G., Garcia De La Banda, M.J., Whisstock, J., 2007, RCPdb: An evolutionary classification and codon usage database for repeat-containing proteins, Genome Research, vol 17, issue 7, Cold Spring Harbor Lab Press, Woodbury NY USA, pp. 1118-1127.

Askew, D.J., Cataltepe, S., Kumar, V., Edwards, C., Pace, S.M., Howarth, R.N., Pak, S.C., Askew, Y.S., Bromme, D., Luke, C., Whisstock, J., Silverman, G.A., 2007, SERPINB11 is a new noninhibitory intracellular serpin: Common single nucleotide polymorphisms in the scaffold impair conformational change, The Journal of Biological Chemistry, vol 282, issue 34, The American Society of Biochemistry & Molecular Biology Inc, Bethesda MD USA, pp. 24948-24960.

Scott, F.L., Sun, J., Whisstock, J., Kato, K., Bird, P.I., 2007, SerpinB6 is an inhibitor of kallikrein-8 in keratinocytes, Journal of Biochemistry, vol 142, issue 4, Japanese Biochemical Society, Tokyo, Japan, pp. 435-442.

Porter, C.J., Schuch, R., Pelzek, A.J., Buckle, A.M., McGowan, S., Wilce, M.C.J., Rossjohn, J., Russell, R., Nelson, D., Fischetti, V.A., Whisstock, J.C., 2007, The 1.6 angstrom crystal structure of the catalytic domain of PlyB, a bacteriophage lysin active against Bacillus anthracis, Journal of Molecular Biology, vol 366, issue 2, Academic Press Ltd, Elsevier Science Ltd, London UK, pp. 540-550.

Zhang, Q., Buckle, A.M., Law, R.H.P., Pearce, M.C., Cabrita, L., Lloyd, G., Irving, J.A., Smith, A.I., Ruzyla, K., Rossjohn, J., Bottomley, S.P., Whisstock, J., 2007, The N terminus of the serpin, tengpin, functions to trap the metastable native state, EMBO Reports, vol 8, issue 7, Nature Publishing Group, UK, pp. 658-663.

Paton, A.W., Beddoe, T.C., Thorpe, C.M., Whisstock, J., Wilce, M.C.J., Rossjohn, J., Talbot, U.M., Paton, J.C., 2006, AB5 subtilase cytotoxin inactivates the endoplasmic reticulum chaperone BiP, Nature, vol 443, issue 7111, Nature Publishing Group, London UK, pp. 548-552.

Law, R.H.P., Zhang, Q., McGowan, S., Buckle, A.M., Silverman, G.A., Wong, W., Rosado, C.J., Langendorf, C., Pike, R.N., Bird, P.I., Whisstock, J., 2006, An overview of the serpin superfamily, Genome Biology, vol 7, issue 5, Biomed Central Ltd, London UK, pp. 216-1-216-11.

Marland, Z., Beddoe, T.C., Zaker-Tabrizi, L., Lucet, I., Brammananth, R., Whisstock, J.C., Wilce, M.C.J., Coppel, R.L., Crellin, P.K., Rossjohn, J., 2006, Hijacking of a substrate-binding protein scaffold for use in mycobacterial cell wall biosynthesis, Journal of Molecular Biology, vol 359, issue 4, Academic Press Ltd Elsevier Science Ltd, London UK, pp. 983-997.

Shen, Y., Cranmer, S.L., Aprico, A., Whisstock, J., Jackson, S., Berndt, M.C., Andrews, R.K., 2006, Leucine-rich repeats 2-4 (Leu60-Glu128) of platelet glycoprotein Ibalpha regulate shear-dependent cell adhesion to von Willebrand factor, The Journal of Biological Chemistry, vol 281, issue 36, American Society for Biochemistry and Molecular Biology, Inc, Bethesda USA, pp. 26419-26423.

Konagurthu, A.S., Whisstock, J.C., Stuckey, P.J., Lesk, A.M., 2006, MUSTANG: a multiple structural alignment algorithm, PROTEINS: Structure, Function, and Bioinformatics, vol 64, issue 3, Wiley-Liss, Div John Wiley & Sons Inc, Hoboken USA, pp. 559-574.

Amin, A.A., Faux, N.G., Fenalti, G., Williams, G.N., Bernadou, A., Daglish, B., Keefe, K., Middleton, S., Rae, J., Tetis, K., Law, R.H.P., Fulton, K.F., Rossjohn, J., Whisstock, J.C., Buckle, A.M., 2006, Managing and mining protein crystallization data, Proteins: Structure Function, and Bioinformatics, vol 62, issue 1, Wiley-Liss, Hoboken USA, pp. 4-7.

Kaiserman, D., Whisstock, J.C., Bird, P.I., 2006, Mechanisms of serpin dysfunction in disease, Expert Reviews in Molecular Medicine, vol 8, issue 31, Cambridge University Press, New York USA, pp. 1-19.

Whisstock, J., Bottomley, S.P., 2006, Molecular gymnastics: serpin structure, folding and misfolding, Current Opinion in Structural Biology, vol 16, issue 6, Current Biology Ltd, London UK, pp. 761-768.

Chow, M.K., Amin, A.A., Fulton, K.F., Whisstock, J.C., Buckle, A.M., Bottomley, S.P., 2006, REFOLD: an analytical database of protein refolding methods, Protein Expression and Purification, vol 46, issue 1, Academic Press Inc Elsevier Science, San Diego USA, pp. 166-171.

Chow, M.K.M., Amin, A.A., Fulton, K.F., Fernando, T., Kamau, L., Batty, C., Louca, M., Ho, S., Whisstock, J.C., Bottomley, S.P., Buckle, A.M., 2006, The REFOLD database: a tool for the optimization of protein expression and refolding., Nucleic Acids Research, vol 34, issue D-Base Issue, Oxford University Press, Oxford UK, pp. D207-D212.

Hennebry, S.C., Law, R.H.P., Richardson, S.J., Buckle, A.M., Whisstock, J.C., 2006, The crystal structure of the transthyretin-like protein from Salmonella dublin, a prokaryote 5-hydroxyisourate hydrolase, Journal of Molecular Biology, vol 359, issue 5, Academic Press Ltd Elseiver Science Ltd, London UK, pp. 1389-1399.

Kaiserman, D., Bird, C.H., Sun, J., Matthews, A.Y., Ung, K.S., Whisstock, J.C., Thompson, P.E., Trapani, J.A., Bird, P.I., 2006, The major human and mouse granzymes are structurally and functionally divergent, The Journal of Cell Biology, vol 175, issue 4, Rockefeller University Press, USA, pp. 619-630.

McGowan, S., Buckle, A.M., Irving, J.A., Ong, P.C., Bashtannyk-Puhalovich, T.A., Kan, W., Henderson, K., Bulynko, Y.A., Popova, E.Y., Smith, A.I., Bottomley, S.P., Rossjohn, J., Grigoryev, S.A., Pike, R.N., Whisstock, J.C., 2006, X-ray crystal structure of MENT: evidence for functional loop-sheet polymers in chromatin condensation, The EMBO Journal, vol 25, issue 13, Nature Publishing Group, New York USA, pp. 3144-3155.

Bird, C.H., Sun, J., Ung, K.S., Karambalis, D., Whisstock, J.C., Trapani, J.A., Bird, P.I., 2005, Cationic sites on granzyme B contribute to cytotoxicity by promoting its uptake into target cells, Molecular and Cellular Biology, vol 25, issue 17, American Society for Microbiology, Washington USA, pp. 7854-7867.

Bird, C.H., Buzza, M.S., Sun, J., Ung, K.S., Whisstock, J.C., Trapani, J.A., Bird, P.I., 2005, Contribution of cationic sites to granzyme B uptake into target cells, Tissue Antigens, vol 66, issue 5, Blackwell Publishing, Oxford England, pp. 362-363.

Kerr, F.K., O'Brien, G., Quinsey, N.S., Whisstock, J., Boyd, S.E., Garcia De La Banda, M.J., Kaiserman, D., Matthews, A.Y., Bird, P.I., Pike, R.N., 2005, Elucidation of the substrate specificity of the C1s protease of the classical complement pathway, Journal of Biological Chemistry, vol 280, issue 47, American Society for Biochemistry and Molecular Biology, Inc., Bethesda USA, pp. 39510-39514.

Faux, N.G., Bottomley, S.P., Lesk, A.M., Irving, J.A., Morrison, J.R., Garcia De La Banda, M.J., Whisstock, J.C., 2005, Functional insights from the distribution and role of homopeptide repeat-containing proteins, Genome Research, vol 15, issue 4, Cold Spring Harbor Lab Press, Woodbury USA, pp. 537-551.

Boyd, S.E., Pike, R.N., Rudy, G.B., Whisstock, J., Garcia De La Banda, M.J., 2005, PoPS: A computational tool for modeling and predicting protease specificity, Journal of Bioinformatics and Computational Biology, vol 3, issue 3, Imperial College Press, London UK, pp. 551-585.

Whisstock, J., Bottomley, S.P., Bird, P.I., Pike, R.N., Coughlin, P.B., 2005, Serpins 2005 - fun between the beta-sheets : Meeting report based upon presentations made at the 4th International Symposium on Serpin Structure, Function and Biology (Cairns, Australia), FEBS Journal, vol 272, issue 19, Blackwell Publishing, Oxford UK, pp. 4868-4873.

Tynan, F.E., Burrows, S.R., Buckle, A.M., Clements, C.S., Borg, N.A., Miles, J.J., Beddoe, T.C., Whisstock, J.C., Wilce, M.C.J., Silins, S.L., Burrows, J.M., Kjer-Nielsen, L., Kostenko, L., Purcell, A.W., McCluskey, J., Rossjohn, J., 2005, T cell receptor recognition of a 'super-bulged' major histocompatibility complex class I-bound peptide, Nature Immunology, vol 6, issue 11, Nature Publishing Group, New York USA, pp. 1114-1122.

Fulton, K.F., Buckle, A.M., Cabrita, L.D., Irving, J.A., Butcher, R.E., Smith, A.I., Reeve, S.B., Lesk, A.M., Bottomley, S.P., Rossjohn, J., Whisstock, J.C., 2005, The high resolution crystal structure of a native thermostable serpin reveals the complex mechanism underpinning the stressed to relaxed transition, Journal of Biological Chemistry, vol 280, issue 9, American Society for Biochemistry and Molecular Biology, Inc., Bethesda USA, pp. 8435-8442.

Law, R.H.P., Irving, J.A., Buckle, A.M., Ruzyla, K., Buzza, M.S., Bashtannyk-Puhalovich, T.A., Beddoe, T.C., Nguyen, K., Worrall, M.D., Bottomley, S.P., Bird, P.I., Rossjohn, J., Whisstock, J.C., 2005, The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helix, Journal of Biological Chemistry, vol 280, issue 23, American Society for Biochemistry and Molecular Biology, Inc., Bethesda USA, pp. 22356-22364.

Buckle, A.M., Devlin, G.L., Jodun, R.A., Fulton, K.F., Faux, N.G., Whisstock, J.C., Bottomley, S.P., 2005, The matrix refolded, Nature Methods, vol 2, issue 1, Nature Publishing Group, London UK, p. 3.

Horvath, A.J., Irving, J.A., Rossjohn, J., Law, R.H.P., Bottomley, S.P., Quinsey, N.S., Pike, R.N., Coughlin, P.B., Whisstock, J., 2005, The murine orthologue of human antichymotrypsin: a structural paradigm for CLADE A3 serpins, Journal of Biological Chemistry, vol 280, issue 52, American Society for Biochemistry and Molecular Biology, Inc., Bethesda USA, pp. 43168-43178.

Ivetac, I., Munday, A.D., Kisseleva, M.V., Zhang, X., Luff, S.E., Tiganis, T., Whisstock, J.C., Rowe, T., Majerus, P., Mitchell, C.A., 2005, The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membrane, Molecular Biology of the Cell, vol 16, issue 5, American Society for Cell Biology, Bethesda USA, pp. 2218-2233.

Quinsey, N.S., Greedy, A.L., Bottomley, S.P., Whisstock, J.C., Pike, R.N., 2004, Antithrombin: in control of coagulation, The International Journal of Biochemistry & Cell Biology, vol 36, issue 3, Pergamon-Elsevier Science Ltd, Kidlington England, pp. 386-389.

Fulton, K.F., Ervine, S., Faux, N.G., Forster, R., Ly, W., Robilliard, L., Sonsini, J., Whelan, D., Whisstock, J.C., Buckle, A.M., 2004, CLIMS: Crystallography Laboratory Information Management System, Acta Crystallographica Section D - Biological Crystallography, vol 60, issue 9, Blackwell Munksgaard, Copenhagen Denmark, pp. 1691-1693.

Irving, J.A., Askew, D.J., Whisstock, J.C., 2004, Computational analysis of evolution and conservation in a protein superfamily, Methods, vol 32, issue 2, Academic Press Inc. Elsevier Science, San Diego USA, pp. 73-92.

Silverman, G.A., Whisstock, J.C., Askew, D.J., Pak, S.C., Luke, C.J., Cataltepe, S., Irving, J.A., Bird, P.I., 2004, Human clade B serpins (ov-serpins) belong to a cohort of evolutionarily dispersed intracellular proteinase inhibitor clades that protect cells from promiscuous proteolysis, Cellular and Molecular Life Sciences, vol 61, issue 3, Birkhauser Verlag AG, Basel Switzerland, pp. 301-325.

Konagurthu, A., Whisstock, J., Stuckey, P., 2004, Progressive multiple alignment using sequence triplet optimizations and three-residue exchange costs, Journal of Bioinformatics and Computational Biology [P], vol 2, issue 4, Imperial College Press, London England, pp. 719-745.

Chow, M.K., Mackay, J.P., Whisstock, J.C., Scanlon, M.J., Bottomley, S.P., 2004, Structural and functional analysis of the Josephin domain of the polyglutamine protein ataxin-3, Biochemical and Biophysical Research Communications, vol 322, issue 3, Academic Press Inc. Elsevier Science, San Diego USA, pp. 387-394.

Benning, L.N., Whisstock, J.C., Sun, J., Bird, P.I., Bottomley, S.P., 2004, The human serpin proteinase inhibitor-9 self-associates at physiological temperatures, Protein Science, vol 13, issue 7, Cold Spring Harbor Laboratory Press, Woodbury USA, pp. 1859-1864.

Kjer-Nielsen, L., Clements, C.S., Purcell, A.W., Brooks, A.G., Whisstock, J.C., Burrows, S.R., McCluskey, J., Rossjohn, J., 2003, A structural basis for the selection of dominant T cell receptors in antiviral immunity, Immunity, vol 18, issue 1, Cell Press, Cambridge USA, pp. 53-64.

Ally, N., Whisstock, J.C., Sieprawskalupa, M., Potempa, J., Le Bonniec, B.F., Travis, J., Pike, R.N., 2003, Characterization of the specificity of arginine-specific gingipains from Porphyromonas gingivalis reveals active site differences between different forms of the enzymes, Biochemistry, vol 42, issue 40, American Chemical Society, Washington USA, pp. 11693-11700.

Law, R.H.P., Smooker, P.M., Irving, J.A., Piedrafita, D., Ponting, R., Kennedy, N.J., Whisstock, J.C., Pike, R.N., Spithill, T.W., 2003, Cloning and expression of the major secreted cathepsin B-Iike protein from juvenile Fasciola hepatica and analysis of immunogenicity following liver fluke infection, Infection and Immunity, vol 71, issue 12, American Society for Microbiology, Washington USA, pp. 6921-6932.

Ludeman, J.P., Pike, R.N., Bromfield, K.M., Duggan, P.J., Cianci, J., Le Bonniec, B., Whisstock, J.C., Bottomley, S.P., 2003, Determination of the P'1, P'2 and P'3 subsite-specificity of factor Xa, International Journal of Biochemistry & Cell Biology, vol 35, issue 2, Pergamon-Elsevier Science Ltd, Oxford England, pp. 221-225.

Welss, T., Sun, J., Irving, J.A., Blum, R., Smith, A.I., Whisstock, J.C., Pike, R.N., Von Mikecz, A., Ruzicka, T., Bird, P.I., Abts, H., 2003, Hurpin is a selective inhibitor of lysosomal cathepsin L and protects keratinocytes from ultraviolet-induced apoptosis, Biochemistry, vol 42, issue 24, American Chemical Society, Washington USA, pp. 7381-7389.

Gurung, R., Tan, A., Ooms, L.M., Mcgrath, M.J., Huysmans, R.D., Munday, A.D., Prescott, M., Whisstock, J.C., Mitchell, C.A., 2003, Identification of a novel domain in two mammalian inositol-polyphosphate 5-phosphatases that mediates membrane ruffle localization: the inositol 5-phosphatase SKIP localizes to the endoplasmic reticulum and translocates to membrane ruffles following, Journal of Biological Chemistry, vol 278, issue 13, American Society for Biochemistry and Molecular Biology Inc., Bethesda USA, pp. 11376-11385.

O'Brien, G., Quinsey, N.S., Whisstock, J.C., Pike, R.N., 2003, Importance of the prime subsites of the C1s protease of the classical complement pathway for recognition of substrates, Biochemistry, vol 42, issue 50, American Chemical Society, Washington USA, pp. 14939-14945.

Quinsey, N.S., Fitton, H.L., Coughlin, P.B., Whisstock, J.C., Dafforn, T.R., Carrell, R.W., Bottomley, S.P., Pike, R.N., 2003, Introduction of a mutation in the shutter region of antithrombin (Phe77 Leu) increases affinity for heparin and decreases thermal stability, Biochemistry, vol 42, issue 34, American Chemical Society, Washington USA, pp. 10169-10173.

Andrews, R.K., Gardiner, E.E., Shen, Y., Whisstock, J.C., Berndt, M.C., 2003, Molecules in focus: glycoprotein Ib-IX-V, The International Journal of Biochemistry & Cell Biology, vol 35, issue 8, Permagon-Elsevier Science Ltd, Kidlington England, pp. 1170-1174.

Springhetti, E.M., Istomina, N.E., Whisstock, J.C., Nikitina, T., Woodcock, C.L., Grigoryev, S.A., 2003, Role of the M-loop and reactive center loop domains in the folding and bridging of nucleosome arrays by MENT, Journal of BIological Chemistry, vol 278, issue 44, American Society for Biochemistry and Molecular BIology Inc., Bethesda USA, pp. 43384-43393.

Irving, J.A., Cabrita, L.D., Rossjohn, J., Pike, R.N., Bottomley, S.P., Whisstock, J.C., 2003, The 1.5 crystal structure of a prokaryote serpin: controlling conformational change in a heated environment, Structure, vol 11, issue 4, Cell Press, Cambridge USA, pp. 387-397.

Irving, J.A., Spithill, T.W., Pike, R.N., Whisstock, J.C., Smooker, P.M., 2003, The evolution of enzyme specificity in Fasciola spp., Journal of Molecular Evolution, vol 57, issue 1, Springer-Verlag, New York USA, pp. 1-15.

Irving, J., Pike, R.N., Dai, W., Bromme, D., Worrall, M.D., Silverman, G.A., Coetzer, T.H., Dennison, C., Bottomley, S., Whisstock, J.C., 2002, Evidence that serpin architecture intrinsically supports papain-like cystein protease inhibition: Engineering 1- abutrypsin to inhibit acathepsin proteases, Biochemistry, vol 41, issue 15, American Chemical Society, Washington DC USA, pp. 4998-5004.

Irving, J.A., Shushanov, S., Pike, R.N., Popova, E.Y., Bromme, D., Coetzer, T.H., Bottomley, S., Boulynko, I.A., Grigoreyev, S.A., Whisstock, J.C., 2002, Inhibitory activity of a heterchromatin-associated serpin (MENT) against papain like cystein proteinases affects chromatin structure and blocks cell proliferation, The Journal of Biological Chemistry, vol 277, issue 15, American Society Biochemistry Molecular Biology Inc, Bethesda MD USA, pp. 13192-13201.

Whisstock, J.C., Shen, Y., Lopez, J.A., Andrews, R.K., Berndt, M.C., 2002, Molecular Modeling of the Seven Tandem Leucine-Rich Repeats within the Ligand-Binding Region of Platelet Glycoprotein Ib, Thrombosis and Haemostatis, vol 87, issue 2, Schattauer GMBH-Verlag, Stuttgart Germany, pp. 329-333.

Quinsey, N.S., Whisstock, J.C., Le Bonniec, B., Louvain, V., Bottomley, S.P., Pike, R.N., 2002, Molecular determinants of the mechanisms underlying acceleration of the interaction between antithrombin and factor Xa by heparin pentasaccharide, The Journal of Biological Chemistry, vol 277, issue 18, American Society of Biochemistry and Molecular Biology, Bethesda MS USA, pp. 15971-15978.

Cabrita, L., Whisstock, J.C., Bottomley, S., 2002, Probing the role of the F-Helix in serpin stability through a single tryptophan substitution, Biochemistry, vol 41, issue 14, American Chemical Society, Washington DC USA, pp. 4575-4581.

Irving, J., Steenbakkers, P.J., Lesk, A.M., Op Den Camp, H.J., Pike, R.N., Whisstock, J.C., 2002, Serpins in Prokaryotes, Molecular Biology and Evolution, vol 19, issue 11, Society Molecular Biology Evolution, Lawrence KS USA, pp. 1881-1890.

Pike, R.N., Bottomley, S., Irving, J., Bird, P.I., Whisstock, J.C., 2002, Serpins: finely balanced conformational traps, IUBMB Life, vol 54, issue 1, Taylor & Francis, Philadelphia PA USA, pp. 1-7.

Prescott, M., Nowakowski, S., Gavin, P., Nagley, P., Whisstock, J.C., Devenish, R.J., 2002, Subunit fusion are functionally incorporated into mitochondrial F1F0 synthase arguing against a rigid cap structure at the top of F1, Journal of Biological Chemistry, vol 278, issue 1, American Society of Biochemistry and Molecular Biology Inc., Bethesda MD USA, pp. 251-256.

McGowan, S., Lucet, I.S., Cheung, J.K., Awad, M.M., Whisstock, J.C., Rood, J.I., 2002, The FxRxHrS motif: a conserved region essential for DNA binding of the VirR response regulator from Clostridium perfringens, Journal of Molecular Biology, vol 322, issue 5, Academic Press Ltd Elsevier Science Ltd, London England, pp. 997-1011.

Al-Khunaizi, M., Luke, C.J., Askew, Y.S., Pak, S.C., Cataltepe, S., Miller, D.R., Mills, D.R., Tsu, C., Bromme, D., Irving, J., Whisstock, J.C., Silverman, G.A., 2002, The serpin SQN-5 is a dual mechanistic-class inhibitor of serine and cysteine proteinases, Biochemistry, vol 41, issue 9, American Chemical Society, Washington DC USA, pp. 3189-3199.

Whisstock, J.C., Wiradjaja, F., Waters, J., Gurung, R., 2002, The structure and function of catalytic domains within inositol polyphosphate 5- phosphates, IUBMB Life, vol 53, issue 1, Taylor & Francis, Philadelphia PA USA, pp. 15-23.

Hearn, M.T.W., Quirino, J.P., Whisstock, J.C., Terabe, S., 2002, Thermal Unfolding of Proteins Studied by Coupled Reversed-Phase HPLC-Electrospray Ionizatin Mass Spectronometry Techniques Based on Isotope Exchange Effects, Analytical Chemistry, vol 74, issue 6, American Chemical Society, Washington DC USA, pp. 1467-1475.

Guerin, J., Gelfi, J., Camus, C., Delverdier, M., Whisstock, J.C., Amardeihl, M., Py, R., Bertagnole, S., Messud-Petit, F., 2001, Characterization and functional analysis of Serp3: a novel myxoma virus-encoded serpin invloved in virulence, Journal of General Virology, vol 82, Society for General Microbiology, Spencers Woodsreading England, pp. 1407-1417.

Nandurkar, H., Caldwell, K., Whisstock, J.C., Layton, M., Gaudet, E., Norris, F., Majerus, P., Mitchell, C.A., 2001, Characterization of an adapter subunit to a phosphatidylinositol (3)P 3-phosphatase: Identification of a myotubularin-related protein lacking catalytic activity, Proceedings of the National Academy of Sciences of the United States of America, vol 98, issue 17, National Academy of Sciences, Washington DC USA, pp. 9499-9504.

Sun, J., Whisstock, J.C., Harriott, P., Walker, B., Novak, A.J., Thompson, P., Smith, A., Bird, P.I., 2001, Importance of the P4' residue in human granzyme B inhibitors and substrates revealed by scanning mutagenesis of the proteinase inhibitor 9 reactive center loop, Journal of Biological Chemistry, vol 276, issue 18, American Society for Biochemistry and Molecular Biology Inc, Bethesda MD USA, pp. 15177-15184.

Matusan, A.E., Kelley, P.G., Pryor, M.J., Whisstock, J.C., Davidson, A.D., Wright, P.J., 2001, Mutagenesis of the dengue virus type 2 NS3 proteinase and the production of growth-restricted virus, Journal of General Virology, vol 82, Society for General Microbiology, UK, pp. 1647-1656.

Irving, J., Whisstock, J.C., Lesk, A.M., 2001, Protein structural alignments and functional genomics, Proteins: Structure function and genetics, vol 42, Wiley-Liss, New York NY USA, pp. 378-382.

Ludeman, J.P., Whisstock, J.C., Hopkins, P., Le Bonniec, B., Bottomley, S., 2001, Structure of a serpin-enzyme complex probed by cysteine substitutions and fluorescence spectroscopy, Biophysical Journal, vol 80, Biophysical Society, Bethesda MD USA, pp. 491-497.

Bottomley, S., Lawrenson, I.D., Tew, D.J., Dai, W., Whisstock, J.C., Pike, R.N., 2001, The role of strand 1 of the C b-sheet in the structure and function of a1- antitrypsin, Protein Science, vol 10, Cold Spring Harbor Laboratory Press, Plainview New York NY USA, pp. 2518-2524.

Silverman, G.A., Bird, P.I., Carrell, R.W., Church, F., Coughlin, P.B., Gettins, P., Irving, J., Lomas, D., Luke, C., Moyer, R., Pemberton, P., Remold-O'Donnell, E., Salvesen, G., Travis, J., Whisstock, J.C., 2001, The serpins are an expanding superfamily of structurally similar but functionally diverse proteins, Journal of Biological Chemistry, vol 276, issue 36, American Society for Biochemistry and Molecular Biology, Bethesda MD USA, pp. 33293-33296.

Wiradjaja, F., Ooms, L.M., Whisstock, J.C., Mccoll, B.K., Helfenbaum, L., Sambrook, J., Gething, M., Mitchell, C.A., 2001, The yeast inositol polyphosphate 5-phosphatase Inp54p localizes to the endoplasmic reticulum via a C-terminal hydrophobic anchoring tail, Journal of Biological Chemistry, vol 276, issue 10, American Society for Biochemsitry and Molecular Biology Inc, Bethesda MD USA, pp. 7643-7653.

Smooker, P.M., Whisstock, J.C., Irving, J., Siyaguna, S., Spithill, T.W., Pike, R.N., 2000, A single amino acid substitution affects substrate specificity in cysteine proteinases from Fasciola hepatica, Protein Science, vol 9, Cambridge University Press, New York NY USA, pp. 1-6.

Dunstone, M.A., Dai, W., Whisstock, J.C., Rossjohn, J., Pike, R.N., Feil, S.C., Le Bonniec, B., Parker, M.W., Bottomley, S.P., 2000, Cleaved antitrypsin polymers at atominc resolution, Protein Science, vol 9, Cambridge University Press, New York NY USA, pp. 417-420.

Whisstock, J.C., Skinner, R., Carrell, R.W., Lesk, A.M., 2000, Conformational changes in serpins: 1. The native and cleaved conformations of alpha1-antitrypsin, Journal of Molecular Biology, vol 296, Academic Press Ltd, London UK, pp. 685-699.

Whisstock, J.C., Pike, R.N., Jin, L., Skinner, R., Pei, X.Y., Carrell, R.W., Lesk, A.M., 2000, Conformational changes in serpins: II. The mechanism of activation of antithrombin by heparin, Journal of Molecular Biology, vol 301, Academic Press Ltd, London UK, pp. 1287-1305.

Myers, M., Davies, J.M., Tong, J.C., Whisstock, J.C., Scealy, M., Mackay, I.R., Rowley, M.J., 2000, Conformational epitopes on the diabetes autoantigen GAD65 identified by peptide phage display and molecular modeling, Journal of Immunology, vol 165 (7), American Association of Immunologists, Bethesda USA, pp. 3830-3838.

Irving, J., Pike, R.N., Lesk, A.M., Whisstock, J.C., 2000, Phylogeny of the serpin superfamily: Implications of patterns of amino acid conservation for structure and function, Genome Research, vol 10 issue 12, Cold Sprin Harbor Laboratory Press, Plainview USA, pp. 1845-1864.

Rowley, M.J., Scealy, M., Whisstock, J.C., Jois, J.A., Wijeyewickrema, L.C., Mackay, I.R., 2000, Prediction of the immunodominant epitope of the pyruvate dehydrogenase complex E2 in primary biliary cirrhosis using phage display, Journal of Immunology, vol 164 issue 1, American Association of Immunologists, Bethesda USA, pp. 3413-3419.

Shen, Y., Romo, G.M., Dong, J., Schade, A., McIntire, L.V., Kenny, D., Whisstock, J.C., Berndt, M.C., Lopez, J.A., Andrews, R.K., 2000, Requirement of leucine-rich repeats of glycoprotein (GP) Ibalpha for shear-dependent and static binding of von Willebrand factor to the platelet membrane GP Ib-IX-V complex, Blood, vol 95, pp. 903*-910.

Luke, C., Schick, C., Tsu, C., Whisstock, J.C., Irving, J., Bromme, D., Juliano, L., Shi, G., Chapman, H.A., Silverman, G.A., 2000, Simple modifications of the serpin reactive site loop convert SCCA2 into a cystein proteinase inhibitor: A critical role for the P3' proline in facilitating RSL cleavage, Biochemistry, vol 39 issue 24, American Chemical Society, Washington USA, pp. 7081-7091.

De Luca, M., Facey, D.A., Favaloro, E.J., Hertzberg, M.S., Whisstock, J.C., McNally, T., Andrews, R.K., Berndt, M.C., 2000, Structure and function of the von Willebrand factor A1 domain: analysis with monoclonal antibodies reveals distinct binding sites involved in recognition of the platelet membrane glycoprotein Ib-IX-V complex and ristocetin-dependent activation, Blood, vol 95 issue 1, American Society for Hematology, Washington USA, pp. 164-172.

Whisstock, J.C., Romero, S., Gurung, R., Nandurkar, H., Ooms, L.M., Bottomley, S.P., Mitchell, C.A., 2000, The inositol polyphosphate 5-phosphatases and the apurinic/apyrimidinic base excision repair endonucleases share a common mechanism for catalysis, Journal of Biological Chemistry, vol 275 issue 47, Amer Soc for Biochem & Molecular Biology, Bethesda USA, pp. 37055-37061.

Sun, J., Bird, C., Buzza, M.S., McKee, K.E., Whisstock, J.C., Bird, P.I., 1999, Expression and purification of recombinant human granzyme B from "Pichia pastoris", Biochemical and Biophysical Research Communications, vol 261, Academic Press, www.idealibrary.com, pp. 251-255.

Sun, J., Bird, C.H., Buzza, M.S., McKee, K.E., Whisstock, J.C., Bird, P.I., 1999, Expression and purification of recombinant human granzyme B from Pichia pastoris, Biochemical and Biophysical Research Communications, vol 261, Academic Press Inc., San Diego CA USA, pp. 251-255.

Gomme, P.T., Thompson, P.E., Whisstock, J.C., Stanton, P., Hearn, M.T., 1999, Identification of functional regions of the thyrotropin beta-subunit recognised by two monoclonal antibodies mAb<279 and mAb<299: a chimetic peptide approach, J Peptide Research, vol 54, Munksgaard International Publishers Ltd, Copenhagen Denmark, pp. 218-229.

Davies, J.M., Scealy, M., Cai, Y.P., Whisstock, J.C., Mackay, I.R., Rowley, M.J., 1999, Multiple alignment and sorting of peptides derived from phage-displayed random peptide libraries with polyclonal sera allows discrimination of relevant phagotopes, Molecular Immunology, vol . 36 issue 1, Pergamon-Elsevier Science Ltd, Kidlington Oxford UK, pp. 659-667.

James, E.L., Whisstock, J.C., Gore, M.G., Bottomley, S.P., 1999, Probing the unfolding pathway of alpha1-antitrypsin, Journal of Biological Chemistry, vol 274, no 14, The American Soc. for Biochem. and Mol. Biol., USA, pp. 9482-9488.

Whisstock, J.C., Lesk, A.M., 1999, SH3 domains in prokaryotes, Trends in Biochemical Sciences, vol 24, Elsevier Science, www.trends.com, pp. 132-133.

Whisstock, J.C., Irving, J., Bottomley, S.P., Pike, R.N., Lesk, A.M., 1999, Serpins in the "Caenorhabditis elegans" genome, Proteins-Structure Function and Genetics, vol 36, Wiley-Liss, Inc., URL:http://www.sanger.ac.uk/Projects/C_elegans/wo/, pp. 31-41.

Gomme, P.T., Thompson, P.E., Whisstock, J.C., Stanton, P., Hearn, M.T., 1999, Use of synthetic peptides to delineate discontinuous sequence regions involved in epitope sites of the thyrotropin beta-subunit, Letters in Peptide Science, vol 6, no 2-3, Kluwer Academic Publishers, Dordrecht Netherland, pp. 185-192.

De Veer, M.J., Sim, H., Whisstock, J.C., Devenish, R.J., Ralph, S.J., 1998, IFI60/ISG60/IFIT4, a new member of the human IFI54/IFIT2 family of interferon-stimulated genes, Genomics, vol 54, Academic Press, San Diego California USA, pp. 267-277.

Bottomley, S.P., Hopkins, P., Whisstock, J.C., 1998, a1 - antitrypsin polymerisation can occur by both Loop A and C sheet mechanisms, Biochemical and Biophysical Research Communications, vol 251 no 1, Academic Press, Orlando FL USA, pp. 1-5.

Conference Proceedings

Faux, N.G., Beitz, A., Bate, M., Amin, A.A., Atkinson, I.M., Enticott, C.M., Mahmood, K., Swift, M.J., Treloar, A.E., Abramson, D.A., Whisstock, J., Buckle, A.M., 2007, eResearch solutions for high throughput structural biology, Third IEEE International Conference on e-Science and Grid Computing, 10-13 December, 2007, IEEE Computer Society, India, pp. 221-227.

Tynan, F.E., Burrows, S.R., Buckle, A.M., Clements, C.S., Borg, N., Beddoe, T.C., Whisstock, J.C., Wilce, M.C.J., Silins, S.L., Burrows, J.M., Kjer-Nielsen, L., Kostenko, L., Purcell, A.W., McCluskey, J., Rossjohn, J., 2005, A super-bulged epitope and single amino acid polymorphism identify critical features for T cell recognition, Tissue Antigens, 29 November 2005 - 3rd December 2005, Blackwell Publishing, Oxford England, p. 571.

Boyd, S.E., Garcia De La Banda, M.J., Pike, R.N., Whisstock, J., Rudy, G.B., 2004, PoPS: a computational tool for modeling and predicting protease specificity, Proceedings of the 2004 IEEE Computational Systems Bioinformatics Conference, 16 August 2004 to 19 August 2004, IEEE Computer Society Press, Los Alamitos USA, pp. 372-381.

Davies, J.M., Scealy, M., Cai, Y.P., Whisstock, J.C., Mackay, I.R., Rowley, M.J., 1999, Application of multiple sequence alignment algorithm PILEUP to cluster peptides derived from screening phage-displayed libraries, Proceedings, 24th Annual Lorne Conference on Protein Structure and Function, Lorne Vic 7-11 February 1999, Lorne Protein Workshop, Inc., Melbourne Vic Australia, p. A41.

Bottomley, S.P., James, E.L., Whisstock, J.C., 1999, Biophysical and biochemical analysis of the alpha1-antitrypsin folding and misfolding pathways, Proceedings, 24th Annual Lorne Conference on Protein and Structure Function, Lorne Vic, Lorne Protein Workshop, Inc., Melbourne Vic Australia, p. B22.

Smith, S.R., Whisstock, J.C., Lawrenson, I.D., Hopkins, P., Ludeman, J., Bottomley, S.P., Pike, R.N., 1999, Engineering a novel factor Xa inhibitor, Proceedings, 24th Annual Conference on Protein Structure and Function, Lorne Vic 7-11 February 1999, Lorne Protein Workshop, Inc., Melbourne Vic Australia, p. B12.

Irving, J., Pike, R.N., Whisstock, J.C., 1999, Substitution of the reactive centre loop of antitrypsin with that of SCCA1 yields a chimeric protein capable of inhibiting cysteine proteinases of the papain superfamily, Proceedings, 24th Annual Lorne Conference on Protein Structure and Function, Lorne Vic 7-11 February 1999, Lorne Protein Workshop, Inc., Melbourne Vic Australia, p. A8.

Lawrenson, I.D., Whisstock, J.C., Pike, R.N., Bottomley, S.P., 1999, The importance of strand 1C in the inhibitory mechanism of alpha1-antitrypsin, Proceedings, 24th Annual Conference on Protein Structure and Function, Lorne Vic 7-11 February 1999, Lorne Protein Workshop, Inc., Melbourne Vic Australia, p. C38.

Whisstock, J.C., Lesk, A.M., Pike, R.N., 1999, The mechanism of anticoagulant activation of antithrombin by heparin, Proceedings, 24th Annual Lorne Conference on Protein Structure and Function, Lorne Vic 7-11 February 1999, Lorne Protein Workshop, Inc., Melbourne Vic Australia, p. C39.

Irving, J., Boyd, S., Pike, R.N., Whisstock, J.C., 1999, The phylogeny of the serpin superfamily, Proceedings, 24th Annual Lorne Conference on Protein Structure and Function, Lorne Vic 7-11 February 1999, Lorne Protein Workshop, Inc., Melbourne Vic Australia, p. C40.

Pike, R.N., Smooker, P.M., Irving, J., Whisstock, J.C., Spithill, T.W., 1998, Characterization of a recombinant Fasciola Hepatica cathepsin L, The Australian Society for Parasitology, Melbourne VIC, 27 Sept - 1 Oct 1998, Australian Society for Parasitology, Melbourne Vic Australia, p. 63.

James, E.L., Whisstock, J.C., Bottomley, S.P., 1998, The unfolding pathway of a1 - antitrypsin probed by florecence spectroscopy, Proceedings, 23rd Annual Lorne Conference on Protein Structure and Function, Lorne VIC 8-12 February 1998, Lorne Protein Workshop, Inc., Melbourne Vic Australia, p. 28.

Gomme, P.T., Thompson, P.E., Whisstock, J.C., Hearn, M.T., 1997, Homology modelling of the glycoprotein hormones: receptor recognition characteristics, 1st International Peptide Symposium, Kyoto, Japan, Nov. 30-Dec. 5, 1997, Kluwer Academic Publishers B.V., Amsterdam Netherlands, p. 122.

Other

Whisstock, J., 2010, 'Assassin' protein a killing machine to salvage our bodies from within, The Age, Fairfax, Melbourne, Australia.

Sofian, T., Horvath, A.J., Law, R.H.P., Whisstock, J., 2007, Monash Researchers Fight Deep Vein Thrombosis, Media Release 13 December 2007, Monash University, Australia.

Postgraduate Research Supervisions

Current Supervision

Program of Study:

(POSTGRADUATE QUALIFYING OR PRELIMINARY).

Supervisors:

Traore, D (Joint-co), Whisstock, J (Joint-co).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

A genetic modifier screen to identify new genes acting downstream of torso-like in Drosophila.

Supervisors:

Warr, C (Main), Whisstock, J (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Extracellular signalling mechanisms involved in patterning the Drosophila embryo.

Supervisors:

Warr, C (Main), Whisstock, J (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Investigations of the pore forming toxin, Listeriolysin O.

Supervisors:

Rosado, C (Main), Traore, D (Associate), Whisstock, J (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Structural and Biophysical studies of MACPF pore-forming toxins, pleurotolysin.

Supervisors:

Dunstone, M (Main), Law, H (Associate), Whisstock, J (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Structural and Functional Analysis of the Interactions of the Interleukin 37 Receptor Complex.

Supervisors:

Rosado, C (Main), Whisstock, J (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Structural and functional studies of plasminogen.

Supervisors:

Law, H (Joint), Whisstock, J (Joint-co).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

Structural aspects of the pore forming protein perforin.

Supervisors:

Buckle, A (Joint), Whisstock, J (Joint-co).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

The role of MACPF proteins in zebrafish development..

Supervisors:

Bird, P (Main), Verkade, H (Associate), Whisstock, J (Associate).

Program of Study:

(DOCTORATE BY RESEARCH).

Thesis Title:

The role of mammalian membrane attack complex/perforin (MACPF) proteins in Neurobiology and Neural development.

Supervisors:

Bird, P (Joint), Whisstock, J (Joint-co).

Completed Supervision

Student:

Bidargaddi Parameshwar, N.

Program of Study:

Hybrid computational models for protein sequence analysis and secondary structure prediction. (PHD) 2006.

Supervisors:

Chetty, M (Main), Kamruzzaman, J (Associate), Whisstock, J (Associate).

Student:

Boyd, S.

Program of Study:

COMPUTATIONAL MODELLING AND PREDICTION OF PROTEASE SPECIFICITY. (PHD) 2005.

Supervisors:

Garcia De La Banda, M (Main), Pike, R (Associate), Whisstock, J (Associate).

Student:

Faux, N.

Program of Study:

The role of single amino acid repeats in protein function and evolution. (PHD) 2008.

Supervisors:

Whisstock, J (Main), Garcia De La Banda, M (Associate), Pike, R (Associate).

Student:

Fenalti, G.

Program of Study:

Structural and functional studies on human glutamic acid decarboxylase. (PHD) 2007.

Supervisors:

Rowley, M (Main), Whisstock, J (Associate), El-Kabbani, O (Associate).

Student:

Irving, J.

Program of Study:

SERPINS: EVOLUTION AND CYSTEINE PROTEINASE INHIBITION. (PHD) 2002.

Supervisors:

Pike, R (Main), Spithill, T (Associate), Whisstock, J (Associate).

Student:

Mahmood, K.

Program of Study:

Computational algorithms for comparative genomics. (PHD) 2012.

Supervisors:

Webb, G (Joint), Whisstock, J (Joint-Co).

Student:

Ong, P.

Program of Study:

Investigation of the chromatin condensation mechanism of the nuclear serpin, MENT. (PHD) 2008.

Supervisors:

Pike, R (Main), Whisstock, J (Joint).

Student:

Waters, J.

Program of Study:

The phosphatidylinositol 3, 4, 5-triphosphate-dependent Rac exchanger, P-Rex1 regulates macropinocytosis and neurite differentiation. (PHD) 2005.

Supervisors:

Mitchell, C (Main), Whisstock, J (Associate).

Student:

Wong, W.

Program of Study:

The structural and biochemical basis for host tissue destruction mediated by subtilisin-like proteases from the ovine footrot pathogen, Dichelobacter nodosus. (PHD) 2012.

Supervisors:

Whisstock, J (Main), Rood, J (Associate).