Dr Aaron Irving - Researcher Profile

Biography

My scientific career began wih a BSc in microbiology/molecular cellular biology at the University of Queensland, followed by a Honours degree in microbiology working on immuno-evasion and viral infections.I continued to work at CICR/DICIMM UQ with a PhD in virology in nigel mcmillans laboratory studying the IFN evasion of chicken pox virus/VZV. Since then I have continued my research focusing on innate immunity and its cross-talk with cellular biology in Bryan Williams laboratory at MIMR / Monash University. In addition I help with the MHRP/MIMR node of Monash MIcroImaging.

Research Activities

PKR, a protein kinase involved in the cellular stress response and translation inhibition, and its role in inflammation, including TLR signalling.
PKR’s role in cellular migration, invasion, cellular machinery and phagocytosis.
Viral infection and innate immunity.

Techniques/Expertise

Mammalian cell culture (epithelial cells, bone marrow derived macrophages, macrophage lines, T cell lines, whole splenocytes)
Immuno-detection techniques (eg. ELISA, Western blotting, flow cytometry)
TLR ligand screening
Gene knockdown (RNAi)
Confocal and live-cell microscopy, fluorescence, deconvolution microscopy, FRET, FRAP, FLIM, BiFC,
Molecular Biology (cloning, mutagenesis, library screening)
Viral infection assays (SFV, Adv5, HSV1, EMCV, RSV, VZV)
Cellular movement (cellular migration, phagocytic uptake)

Keywords

Innate immunity, viral infections, herpes virus, , Live cell microscopy, confocal,, VZV, chicken pox, immunology, microscopy, , bifunctional sirnas; innate immunity; rna interference; toll-like receptors, deconvolution microscopy, FRET, FRAP, FLIM, BiFC,, genetic screening interferon signal transduction viral genes, innate immunity, innate immunity viral nucleic acids dna-dependent activator of interferon regulatory factor retin, molecular biology, cell biology, , tlr8; splice variants; phagocytosis; helicobacter pylori; innate immunity, virus infection

Qualifications

DOCTOR OF PHILOSOPHY

Institution: University of Queensland

Year awarded: 2010

PHD - RESEARCH HIGHER DEGREE STUDIES - MOLECULAR V

Institution: University of Queensland

Year awarded: 2007

BACHELOR OF SCIENCE (HONORS)

Institution: University of Queensland

Year awarded: 2002

Publications

Journal Articles

Irving, A., Wang, D., Vasilevski, O., Latchoumanin, O., Kozer, N., Clayton, A., Szczepny, A., Morimoto, H., Xu, D., Williams, B., Sadler, A., 2012, Regulation of actin dynamics by protein kinase R control of gelsolin enforces basal innate immune defense, Immunity [P], vol 36, issue 5, Cell Press, United States, pp. 795-806.

Gantier, M., McCoy, C., Rusinova, I., Saulep, D., Wang, D., Xu, D., Irving, A., Behlke, M., Hertzog, P., Mackay-Fisson, F., Williams, B., 2011, Analysis of microRNA turnover in mammalian cells following Dicer1 ablation, Nucleic Acids Research [P], vol 39, issue 13, Oxford University Press, United Kingdom, pp. 5692-5703.

Gantier, M.P.M., Irving, A.T., Liaskos, M., Xu, D., Evans, V., Cameron, P.U., Bourne, J.A., Ferrero, R.L., John, M., Behlke, M.A., Williams, B.R.G., 2010, Genetic modulation of TLR8 response following bacterial phagocytosis, Human Mutation [P], vol 31, issue 9, John Wiley & Sons, Inc., USA, pp. 1069-1079.

Gantier, M.P.M., Tong, S., Behlke, M.A., Irving, A.T., Lappas, M., Nilsson, U.W., Latz, E., McMillan, N.A.J., Williams, B.R.G., 2010, Rational design of immunostimulatory siRNAs, Molecular Therapy [P], vol 18, issue 4, Nature Publishing Group, USA, pp. 785-795.

Irving, A.T., Williams, B.R.G., 2009, Latest advances in innate antiviral defence, F1000 BIOLOGY REPORTS [E], vol 1, issue 22, Biology Reports Ltd, London UK, pp. 1-4.

Chen, J., Irving, A.T., McMillan, N.A.J., Gu, W., 2007, Future of RNAi-based therapies for human papillomavirus-associated cervical cancer, Future Virology [P], vol 2, issue 6, Future Medicine Ltd., UK, pp. 587-595.

Gu, W., Putral, L.N., Irving, A.T., McMillan, N.A.J., 2007, The development and future of oligonucleotide-based therapies for cervical cancer, Current Opinion in Molecular Therapeutics [P], vol 9, issue 2, Thomson Scientific, UK, pp. 126-131.

Clarke, D.T.W., Irving, A.T., Lambley, E.H., Payne, E., McMillan, N.A.J., 2004, A novel method for screening viral interferon-resistance genes, Journal of Interferon and Cytokine Research [P], vol 24, issue 8, Mary Ann Liebert, Inc. Publishers, USA, pp. 470-477.

Languages

• English

Citizenship

• Australian citizen