Monash Biomedical Proteomics Facility

For full technical
information visit
Monash Biomedical Proteomics Facility

Contact us

+61 3 9902 9323
Level 2, Building 77
Monash University Clayton

Key contacts

Dr David Steer
Manager
Monash Biomedical Proteomics Facility
+61 3 9902 9323
david.steer@monash.edu

Professor Ian Smith
Director
Monash Biomedical Proteomics Facility
+61 3 9902 4050
ian.smith@monash.edu

Mass spectrometry (MS) of proteins (proteomics) is one of the fundamental tools for identifying, quantifying and sequencing proteins.

Proteomics helps researchers to understand the roles of proteins in disease. It can also help to identify potential markers of disease, and targets for therapy.

Because we are a fully-automated facility, our suite of MS instrumentation operates at maximum efficiency. This means we are capable of running 24 hours a day.

We offer protein sequencing, a fail-safe technique that allows us to identify and determine the precise sequence of amino acids in any given protein.

We also offer 2D SDS gel analysis. This helps provide greater clarity into complex samples.

Our qualified staff are well-equipped to carry out the wide variety of proteomics services we offer.

Services

  • Protein purification from a variety of sources
  • High sensitivity protein identification with N terminal sequencing
  • HPLC and 2D Gels for protein separation
  • Mass Spectometry for proteomic and quantitative proteomic analyses (DIGE/mass tagging), analysis of intact proteins
  • Image Analysis of 2D gels

Core facilities

  • Applied Biosystems 4700 Proteomics Discovery System (MALDI TOF TOF) for protein identification and mass fingerprinting
  • 2D gel electrophoresis for separating complex protein mixtures
  • Capillary LC and HCT Ultra Ion Trap for protein identification and post translational analysis
  • Nano LC and Micro TOF/q and QQQ systems for high accuracy mass spectrometry and protein quantitation
  • Applied Biosystems Procise Protein Sequencer for N terminal sequencing of picomole quantities of protein or peptide

Access details

You can access this facility via:

  • Collaborative research
  • Fee for service