Faculty of Science

Undergraduate - Unit

This unit entry is for students who completed this unit in 2013 only. For students planning to study the unit, please refer to the unit indexes in the the current edition of the Handbook. If you have any queries contact the managing faculty for your course or area of study.

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6 points, SCA Band 3, 0.125 EFTSL

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FacultyFaculty of Science
OfferedClayton First semester 2013 (Day)
Coordinator(s)Dr Kim Murphy


This unit aims to introduce students to practical aspects of laboratory immunology. The course is laboratory based with tutorials to ensure a thorough theoretical understanding of techniques. Topics include the anatomy of the immune system, production of polyclonal antisera and monoclonal antibodies, structure and properties of immunoglobulins (antibodies), electrophoresis and other techniques for detection of antigen/antibody reactions. Diagnostic immunology techniques include immunofluorescence, fluorescence microscopy, flow cytometry, enzyme linked immunosorbent assay (ELISA), radioimmunoassay (RIA) and analysis of plasma proteins (immunoglobulins, complement etc).


On completion of this unit students will be able to:

  1. Compare different methods of antibodies purified from a range of different biological sources;

  1. Validate different methods of testing purified antibody products;

  1. Demonstrate the ability to perform common laboratory calculations and use these in a practical setting;

  1. Accurately record, analyse and report experimental outcomes and demonstrate this by producing written laboratory reports;

  1. Source relevant scientific literature from a variety of sources, including (but not limited to) scientific journals, text-books and the internet for the completion of written or oral assignments.


Practical class reports: 25%
One assignment: 15%
One 3-hour practical/written examination: 60%

Chief examiner(s)

Contact hours

One 5-hour practical class per week



IMM3031 (highly recommended)